• Journal of Periodontal and Implant Science
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• 제40권5호
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• pp.220-226
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• 2010

Purpose: This study was performed to evaluate the periodontal wound healing effect of particulate equine bone mineral on canine alveolar bone defects. Methods: Twelve adult male beagle dogs were used as study subjects. The mandibular second and fourth premolars were extracted prior to the experimental surgery, and the extraction sites were allowed to heal for 8 weeks. After periodontal probing, two-walled defects were created at the mesial and distal sides of the mandibular third premolars bilaterally, and the defects were filled with equine particulate bone with collagen membrane or bovine particulate bone with collagen membrane, or collagen membrane alone. The defects without any treatment served as negative controls. After probing depth measurement, animals were sacrificed at 10, 16, and 24 post-surgery weeks for micro-computed tomographic and histomorphometric analysis. Results: The equine particulate bone-inserted group showed significantly decreased values of probing depth and first bone contact compared to the negative control and collagen membrane alone groups at weeks 10, 16, and 24 (P<0.05). There were no significant differences in the new cementum length, newly-formed bone area, or newly-formed bone volume between equine particulate bone- and bovine particulate bone-inserted groups, both of which showed significantly increased values compared to the negative control and collagen membrane alone groups (P<0.05). Conclusions: Equine particulate bone showed significant differences in probing depth, first bone contact, new cementum length, newly formed bone area, and bone volume fraction values when compared to the negative control and collagen membrane alone groups. There were no significant differences between equine and bovine particulate bone substitutes in these parameters; therefore, we can conclude that equine particulate bone is equivalent to bovine bone for periodontal regeneration.

• Journal of Periodontal and Implant Science
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• 제35권3호
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• pp.719-730
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• 2005

The purpose of this study was to compare the effect of tetracycline HCL, Citric acid and PrefGel applied on the root surfaces that planed with periodontal curret with Roto bur. In this study, 20 extracted teeth with advanced periodontal disease were used. The teeth were root planing with periodontal curette and Roto bur. Following root planing, each agents was burnished on the prepared root surface for 3 minutes to find opened dentinal tubules. And then, each specimens were investigated using scanning electron microscope. Amount of remained cementum by loss of tooth substance index and the number of opened dentinal tubules were evaluated to each specimens The results were as follows. 1. Groups treated with periodontal curette were almost seemed no removed. Other groups treated with Roto bur showed partially opened dentinal tubule orifices. 2. Loss of tooth substance index were compared between groups. There was no statistically difference between periodontal curette groups. Between Roto bur groups was alike. But there were statistically differences between periodontal curette and Roto bur groups. 3. At comparing with various root conditioning agents, Tetracycline HCL group took statistically higher than Citric acid and PrefGel in opened dentinal tubules. On the other hand, there was no statistically difference between Citric acid group and PrefGel group. As a result of this study, groups treated with Roto bur showed more cementum removed than groups treated with periodontal curette. In a treatment for regeneration of periodontal tissue, it was regarded that Roto bur should be used and that Tetracycline HCL would be more effective as chemical root conditioning agent.

• Restorative Dentistry and Endodontics
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• 제18권1호
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• pp.205-213
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• 1993

The purpose of the study was to evaluate the degree of the marginal leakage of esthetic restorative materials on root caries restoration. 120 cavities were prepared to $90^{\circ}$ butt joint on all margins on the crown and root portion, and divided into 4 groups. The four groups of cavity were filled with Amalgam(Dongmuyung Dental Alloy Co., Ltd, KOREA), Silux$^{(R)}$(3M Co., USA)-Scotch Bond 2$^{(R)}$(3M Co., USA), Silux$^{(R)}$-All Bond$^{(R)}$(BISCO USA), and GC Fuji II$^{(R)}$(G-C Co., JAPAN) respectively. The apical margin of the preparation was finished to leave a flash of restorative material. The coronal margin of the preparation was finished not to leave a flash of restorative material. All specimens were sectioned longitudinally with Isomet Low speed saw(Buether Ltd, USA). The degree of dye penetration was evaluated as the parameter of marginal leakage under the stereoscope. The results were as follows. 1. At the enamel and dentin/cementum margins, the margin were finished to leave a flash of material showed less marginal leakage than that were finished not to leave a flash of material (P<0.001). 2. The enamel margins showed less marginal leakage than the dentin/cementum margins(P<0.001). 3. There was no significant difference in the degree of the marginal leakage between Silux$^{(R)}$-Scotch Bond 2$^{(R)}$ group and Silux$^{(R)}$-All Bond$^{(R)}$ group.

• Restorative Dentistry and Endodontics
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• 제21권2호
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• pp.489-504
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• 1996

The purpose of this study was to compare the adaptation to the tooth structure of five light cured glass ionomer cements (Fuji II LC. VariGlass VLC, Vitremer, Dyract and Geristore). Human, non-carious fifty extracted permanent premolars stored in normal saline were used. Class V cavity preparations were created on the buccal surfaces. The occlusal margin of each cavity was placed on the enamel and the gingival margin was placed on the cementum/dentin. The teeth were then distributed at random into five groups of 10 teeth each. Group 1: Fuji II LC, Group 2 : VariGlass VLC, Group 3 : Vitremer, Group 4: Dyract, Group 5 : Geristore. The prepared cavities were restored with one of the five light cured glass ionomer cements. The manipulation of each material was handled according to the manufacturer's instructions. All samples were placed in incubator of 100% relative humidity at $37^{\circ}C$ for 24 hours. The roots of the teeth were removed with the tapered fissure bur and the remaining crowns were sectioned buccolingually through the center of restorations. The cut interfaces were gradually hand polished on sandpapers from 300 up to 1200 grit. The adaptation at the tooth/cements interface was assessed by SEM (JSM-840A, JEOL Ltd.). The results of this study were as follows : 1. Group 2 revealed the best adaptation and groups 1, 4 and 5 revealed similar adaptation pattern to the cavity walls. Group 3 revealed the worst adaptation to the cavity walls. 2. Enamel margins showed better adaptation than dentin/cementum margins with each material except group 3. 3. The hybrid layers were observed between the glass ionomer cement and dentin in groups 2, 4 and 5.

• Journal of Periodontal and Implant Science
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• 제29권4호
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• pp.873-893
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• 1999

The ultimate goal of periodontal therapy is the regeneration of periodontal tissue and repair of function. For more than a decade there have been many efforts to develop materials and bioactive molecule(such as growth factor and differentiation factors) to promote periodontal wound healing. Among the bioactive molecules, bone morphogenetic protein(BMP) was studied for periodontal wound healing. Since Urist demonstrated that demineralized bone matrix could induce the formation of cartilage and bone in ectopic site, many studies on BMP have been reported. Among those BMPs, it was reported that rhBMP-2 enhanced the healing of bone defects in animal studies and clinical studies. However, its efficacy in periodontal regeneration, especially 1-wall intrabony defects is still unknown. The purpose of this study was to examine the effect of rhBMP-2/ACS on the epithelial migration, gingival connective tissue adhesion, cementum formation, alveolar bone regeneration in intrabony defects of dogs. Four millimeter deep and four millimeter wide 1-wall defects were surgically created in the mesial aspects of the 3rd incisors. The test group received rhBMP-2/ACS with a flap procedure and the control underwent buffer/ACS with a flap procedure. Histologic analysis after 8 weeks of healing revealed the following results: 1. The length of epithelial growth(the distance from alveolar crest to the apical end of JE) was $0.9{\pm}1.5mm$ in the control group and $1.2{\pm}1.4mm$ in the test group. There was no statistically significant difference between the two groups. 2. The length of connective tissue adhesion was $2.4{\pm}1.3mm$ in the control group and $1.2{\pm}1.1mm$ in the test group. The control group showed significantly enhanced adhesion(P<0.05). 3. The length of new cementum was $0.9{\pm}1.0mm$ in the control group and $1.7{\pm}0.8mm$ in the test group. The test group showed significantly enhanced cementum regeneration(P<0.05). 4. The length of new bone height was $1.9{\pm}0.6mm$ in the control group and $2.4{\pm}0.9mm$ in the test group. There was no statistically significant difference between the two groups. 5. The new bone area was $4.7{\pm}1.7mm^2$ in the control group and $8.0{\pm}2.0mm^2$ in the test group. The test group showed significantly enhanced bone formed area(P<0.05). 6. The new bone density was $73.0{\pm}8.6%$ in the control group and $66.6{\pm}15.3%$ in the test group. There was no statistically significant difference between the two groups. These results suggest that the use of rhBMP-2 in 1-wall intrabony defects has significant effect on new cementum and new bone formation area, but doesn't have any significant effect on the prevention of junctional epithelium migration and new bone formation height.

• Journal of Periodontal and Implant Science
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• 제23권3호
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• pp.577-594
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• 1993

Polytetrafluoroethylene membrane 및 millipore filter를 섬유소와 병용사용하여 성견 치주조직 재생에 미치는 영향에 관해 알아보기 위하여 생후 9개월 이상된 잡종성견7마리에 치은박리소파수술후 PTFE membrane를 삽입한 군을 대조군으로, PTFE membrane에 섬유소를 첨가했는 군을 실험1군으로, 수술시 millipore filter를 봉합에 의해 고정시킨군을 실험2군으로, 섬유소로 millipore filter를 고정시킨 군을 실험3군으로 하였다. 술후 각 1, 2, 4, 8, 12 주에 관류고정과 아울러 희생시켜 악골을 채취하고 통법에 의해 후고정, 탈회, 탈수과정을 거쳐 파라핀으로 포매한 후 $4-6{\mu}m$두께의 협, 설측 박편을 제작한 후 H & E 및 Masson’s trichrome염색 후 광학현미경으로 치유결과를 조직학적으로 비교 관찰하여 다음과 같은 결과를 얻었다. 1 주때 대조군 및 실험각군 모두 치은상피조직의 근단이동이 억제된 양상을 나타내었으며, millipore filter를 삽입한 군에서는 섬유소보다 봉합에 의해 막을 고정시킨 경우 술후 1주때 부터 치은퇴축으로 인한 막의 노출이 관찰되었고 술후 4주때 막의 내, 외면에 긴접합상피조직의 근단이동을 관찰할 수 있었으며, PTFE membrane 경우는 섬유소유무에 따른 차이는 관찰되지 않았다. 대조군 및 실험각군 모두 술후 1주때 골양조직을 관찰할 수 있었고 4주때 성숙된 치조골 양상을 보였으며 특히 PTFE membrane인 경우 증가된 협설 측 폭경을 관찰할 수 있었으며, 술후 12주때 실험각군 모두 치조골이 더욱 성숙된 양상을 관찰할 수 있었다. 술후 4주때 대조군, 실험각군 모두 활택된 치근면상에 미약한 신생백악질 및 불규칙적인 교원섬유의 배열을 관찰할 수 있었고 8주때 뚜렷한 백악질의 증가와 12주때에는 신생백악질과 신생치조골 사이에 기능적으로 배열된 교원 섬유를 관찰할 수 있었다. 결론적으로, 섬유소로 막을 고정시킬 경우 치근면과의 긴밀한 접촉을 유지시킬수 있으며, 차단막을 근단쪽으로 위치시켜 막의 노출 위험을 감소시킬수 있으며, 특히 PTFE membrane인 경우 봉합대체물로의 연이은 연구가 필요할 것으로 사료된다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제12권1호
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• pp.148-157
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• 1990

The cementomas are derived from the periodontal ligament and a diversified group of nonrelated lesions producing cementum-like material. The diagnostic term, Cementoma, has encompassed several unrelated lesions : periapical cemental dysplasia, benign (true) cementoblastoma, cementifying fibroma and familial multifle (gigantiform) cementoma. The authors treated one case of benign cementoblastoma & the other of cementifying fibroma by conservative enucleation & curettage. By follow up check of the patient, We obtained of good result without any signs of recurrence of the lesions.

• 대한치과의사협회지
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• 제21권2호통권165호
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• pp.167-172
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• 1983

This study was to investigate the effects of mynol cement on the periapical tissues of 40 molars in ten dogs. Root canal fillings were placed with mynol cement and the animals were killed at different post operative periods ranging from 1 to 5 weeks. The teeth examined on the basis of microscipic findings. The following results may be drawn; 1. 1 week after root canal fillings, the necrosis of cementum and dentin including alveolar bone was to be seen. There were severe inflammatory changes in the periodontal ligament. 2. 2 weeks after root canal fillings, edematous changes and fibrosis in the periodontal ligament were revealed. 3. After 3-4 weeks, fibrosis was more prominent than 2 weeks. 4. 5 weeks after root canal fillings, the osteoblastic activity was found abundantly surrounding the alveolar bone.

• Imaging Science in Dentistry
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• 제50권1호
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• pp.65-71
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• 2020

Ossifying fibroma is a slow-growing benign neoplasm that occurs most often in the jaws, especially the mandible. The tumor is composed of bone that develops within fibrous connective tissue. Some ossifying fibromas consist of cementum-like calcifications, while others contain only bony material; however, a mixture of these calcification types is commonly seen in a single lesion. Of the craniofacial bones, the mandible is the most commonly involved site, with the lesion typically inferior to the premolars and molars. Ossifying fibroma of the jaw shows a female predominance. Some reports of ossifying fibroma have been published in the literature; however, this report continues the research on this topic by detailing 3 types of ossifying fibroma findings on panoramic radiographs and cone-beam computed tomographic images of 4 patients. The radiographs of the presented cases could help clinicians understand the variations in the radiographic appearance of this lesion.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제38권6호
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• pp.321-325
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• 2012

Collagen is widely used for regenerative therapy and pharmaceutical applications as one of the most useful scaffolds. Collagen is the most abundant protein in vertebrates and the natural substrate of various types of animal cells. Bone and dentin are mineralized tissues and almost similar in chemical components. They consist of collagen (18%), non-collagenous proteins (2%), hydroxyapatite (70%) and body fluid (10%) in weight volume. Pepsin-digested, type I collagen (atelocollagen) and heat-denatured collagen (gelatin) are basic collagenous materials for medical use. Demineralized dentin matrix (DDM) and demineralized bone matrix (DBM) belong to acid-insoluble group, and vital tooth-derived DDM is a unique dentin material including cementum and growth factors. In this review, collagen-based materials will be introduced and discussed for bone regenerative surgery.