• Molecules and Cells
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• 제43권11호
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• pp.899-908
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• 2020

Intrinsically disordered proteins or regions (IDPs or IDRs) are widespread in the eukaryotic proteome. Although lacking stable three-dimensional structures in the free forms, IDRs perform critical functions in various cellular processes. Accordingly, mutations and altered expression of IDRs are associated with many pathological conditions. Hence, it is of great importance to understand at the molecular level how IDRs interact with their binding partners. In particular, discovering the unique interaction features of IDRs originating from their dynamic nature may reveal uncharted regulatory mechanisms of specific biological processes. Here we discuss the mechanisms of the macromolecular interactions mediated by IDRs and present the relevant cellular processes including transcription, cell cycle progression, signaling, and nucleocytoplasmic transport. Of special interest is the multivalent binding nature of IDRs driving assembly of multicomponent macromolecular complexes. Integrating the previous theoretical and experimental investigations, we suggest that such IDR-driven multiprotein complexes can function as versatile allosteric switches to process diverse cellular signals. Finally, we discuss the future challenges and potential medical applications of the IDR research.

• 한국ITS학회 논문지
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• 제5권2호
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• pp.29-43
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• 2006

교통정보 제공방법중의 하나로서 기 제공되고 있는 고속도로 ARS서비스는 주행 중 소통정보를 제공하는 유용한 방법이나 하나의 정보를 제공받기 위하여 여러 번의 핸드폰 조작이 요구된다. 그러나 운전 중 휴대폰의 사용은 도로교통법에 의거 위법사항 일뿐만 아니라 운전자의 집중력을 분산시킴으로써 집중력 저하구간을 발생시켜 안전문제에 심각한 영향을 주고 있다. 따라서, 본 연구는 기존의 ARS교통정보 서비스의 제공방식에 대한 문제점을 분석하여 개선된 문제점을 바탕으로 보다나은 교통정보 제공 형태를 제시하기 위하여 기 제공되고 있는 고속도로 ARS이용현황 자료와 고속도로 구간속도자료를 수집 분석하여 서비스의 문제점 도출 및 개선된 보다 나은 교통정보 제공 형태를 제시하였다. 또한, 선호도 조사기법을 통하여 분석된 두 방식 간 안전성과 편리성을 비교함으로써 제시된 제공형태의 효과를 검증하고, 비시장재의 가치 측정 방법인 조건부가치 측정법 (CVM)을 도입하여 이용자들의 지불의사 금액을 바탕으로 개선된 교통정보 제공 형태의 가치를 측정하였다.

• 한국ITS학회 논문지
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• 제17권1호
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• pp.100-113
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• 2018

네트워크 수요량 향상 방안 중 가장 주목받고 있는 Proximity Service는 주파수 사용의 효율성 증대를 위해 대부분 주파수 재사용 방식을 사용한다. 그 결과 셀 edge에서 셀룰러 사용자의 Proximity Service 사용자의 셀 간 간섭 문제가 발생한다. 본 논문에서는 eNB에 Proximity Function의 기능과 파라미터를 새롭게 정의하고, ProSe 파라미터와 ProSe user equipment에 대한 정보를 X2 인터페이스를 통해 인접 셀과 교환하게 함으로써 셀 간섭 완화 방안을 제안한다. 우선 ProSe 탐색 과정에서 주파수 센싱을 통해 셀 간 간섭 문제를 일으키지 않을 자원을 할당한다. 그리고 ProSe 통신 상황에서는 ProSe application code, ProSe application QoS, ProSe application ID의 validity timer를 기반으로 간섭을 일으키지 않을 적합한 자원을 재 할당함으로써 셀 간 간섭 문제를 해결한다.

• 한국ITS학회 논문지
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• 제8권3호
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• pp.131-138
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• 2009

본 연구의 목적은 CA(Cellular Automata)이론을 기반으로 행위자기반(agent-based) 보행시뮬레이션 모델을 개발하고, 개발된 모델을 보행시설 설치 실험에 적용하여보는 것이다. 이를 위하여 본 연구에서는 보행교통류에 CA이론이 적용된 모델을 검토하고, Fence라는 보행시설이 보행자의 통행속도에 미치는 영향을 분석하기 위해 실험을 계획하고 CA보행자 모델을 구축하였다. 구축된 CA보행자 모델은 초기화, 보행자 발생 유입, 측방이동, 전방이동의 하위모델로 구성되어 있다. 실험결과 Fence를 설치했을 때의 보행속도가 Fence를 설치하지 않았을 때의 보행속도보다 높게 나타나, 통행 방향이 다른 보행자가 혼재되어 있을 경우 Fence를 설치하지 않았을 때의 보행속도보다 높게 나타나, 통행 방향이 다른 보행자가 혼재되어 있을 경우 Fence를 설치하는 것이 효과적인 것으로 나타났다. 또한, Fence의 설치는 매우 혼잡한 상황에서보다 적당한 혼잡상태에서 더 큰 것으로 분석되었다.

• 한국ITS학회 논문지
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• 제16권6호
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• pp.195-207
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• 2017

처음 스마트폰이 출시된 이후 다양한 모바일 디바이스들의 발전으로 모바일 트래픽의 사용이 폭발적으로 증가하고 있다. 특히 개인 방송, TV 콘텐츠를 지원하는 OTT(On-The-Top) 서비스가 발전하고 이를 이용하는 사용자가 많아질수록 높은 전송률을 요구하는 영상 트래픽이 증가하게 되어 망의 과부화가 발생할 수밖에 없다. 본 논문에서는 D2D(Device-to-Device) 통신과 위치기반 서비스를 이용하여, OTT 서비스 트래픽에서 발생하는 망 과부화를 해결할 수 있는 방안을 제시하였다. 무선통신망의 OTT 서비스 트래픽을 효과적으로 처리하기 위하여 기존 셀룰러 네트워크에 위치기반 서비스와 D2D 단말 관리를 수행할 수 있는 D2DS(D2D Server)와 LCS(Location Server) 기능블록을 추가하였다. D2DS와 LCS를 통해 OTT 서비스에서 콘텐츠 제공자나 수신자의 역할을 하는 사용자에게 위치기반 D2D를 제공할 수 있는 절차를 추가로 제안하였다.

• 미생물학회지
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• 제6권2호
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• pp.54-62
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• 1968

The effect of gamma-ray on yeast cells Sacch. cerevisiae, and the leakage of cellular constituents such as carbohydrates, ribose, amino acids, inorganic phosphates and organic phosphates have been studied. The samples of yeast cells washed throughly and starved intensively, radiation effects were compared with those of control (un-starved), the irradiation dose rates are in the range from 24 Kr. up. to 480, Kr. The loss of 260m$\mu$. absorbing material, are also observed. Mechanisms of membrane damage by gamma-irradiation are discussed corelating to permeability changes and loss of substances, then active and passive transport process are also under considerations in discussion. The experimental results are as follows, 1. Carbohydrates of yeast cell leak out by gamma-irradiation, and amounts of loss increase proportionally as the increasing of radiation dose, curve of carbohydrates loss in starved cells is parallel with those of non-starved cells. 2. Ribose leak out less than that of carbohydrate from irradiated cell, the dose response curve of loss is straight and proportional to the increasing of radiation doses, slope of the curve is much lower than of carbohydrates. 3. Amino acids also leak out and the curve of losses to radiation is not proportional, it is revealed that there are little losses from yeast at lower doses of irradiation. 4. The losses of inorganic phosphates increase unproportionally to the increasing of irradiation doses, there are little leakage at the lower doses of irradiation. The losses of organic phosphates increase proportionally to the increasing of irradiation doses, and the amount of losses are much more than that of inorganic phosphate at lower doses of irradiation. 5. Leakage from irradiated yeast cells was shown to be due to passive transport process not an energy requiring process of ion transport. 6. Loss of 260 m$\mu$. absorbing material is little more than that of control yeast by the gamma-irradiation dose of 120K.r. and 240K.r.

• Molecules and Cells
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• 제45권11호
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• pp.855-867
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• 2022

For proper function of proteins, their subcellular localization needs to be monitored and regulated in response to the changes in cellular demands. In this regard, dysregulation in the nucleocytoplasmic transport (NCT) of proteins is closely associated with the pathogenesis of various neurodegenerative diseases. However, it remains unclear whether there exists an intrinsic regulatory pathway(s) that controls NCT of proteins either in a commonly shared manner or in a target-selectively different manner. To dissect between these possibilities, in the current study, we investigated the molecular mechanism regulating NCT of truncated ataxin-3 (ATXN3) proteins of which genetic mutation leads to a type of polyglutamine (polyQ) diseases, in comparison with that of TDP-43. In Drosophila dendritic arborization (da) neurons, we observed dynamic changes in the subcellular localization of truncated ATXN3 proteins between the nucleus and the cytosol during development. Moreover, ectopic neuronal toxicity was induced by truncated ATXN3 proteins upon their nuclear accumulation. Consistent with a previous study showing intracellular calcium-dependent NCT of TDP-43, NCT of ATXN3 was also regulated by intracellular calcium level and involves Importin α3 (Imp α3). Interestingly, NCT of ATXN3, but not TDP-43, was primarily mediated by CBP. We further showed that acetyltransferase activity of CBP is important for NCT of ATXN3, which may acetylate Imp α3 to regulate NCT of ATXN3. These findings demonstrate that CBP-dependent acetylation of Imp α3 is crucial for intracellular calcium-dependent NCT of ATXN3 proteins, different from that of TDP-43, in Drosophila neurons.

• Journal of Nutrition and Health
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• 제33권6호
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• pp.660-667
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• 2000

Previous studies on the effect of age on intestinal taurine transport in animals have invariably shown a decline in the activity of the transport system with increasing age. In the present study changes in taurine transporter activity were observed during cell differentiation in the human colon carcinoma cell line HT-29 This cell line exhibits various enterocytic characteristics when differentiated and therefore has frequently been used to study the characteristcs and regulation of nutrient and drug absorption in the small intestine at the cellular level. Pre-treatment of the cells with $\beta$-alanine(10mM) reduced the taurine transport activity to 33% of the value for the control cells(p<0.05) which implies that taurine and $\beta$-alanine share a common $\beta$-amino acid transport system for their celluar uptake in the HI-29 was continued until 21 days post seeding. Kinetic studies of the taurine transporter were conducted in the HT-29 cell line with varying taurine concentration(5-60$\mu$M) in the uptake medium Both Vmax and the Michaelis-Menten constant(Km) of taurine transporter were decreased as differentiation of the HT-29 cell line was progressed ; Vmax of the taurine transporter in cells incubated for 4, 14 and 21 days post seeding was 2.79$\pm$3.4m 16.89$\pm$1.74, and 0.85$\pm$0.08 and 0.32$\pm$0.01nmol.mg protein-1 .30min-1 respectively(p<0.001) and Km was 42.3$\pm$3.4, 16.89$\pm$1.74, and 11.2$\pm$3.0$\mu$M respectively (p<0.01) These results indicate that the activity of sodium dependent active taurine transport system in the HT-29 cell line is decreased as confluent cells are differentiated. This phenomenon in cell culture system corresponds well with the earlier observation of lower intestinal taurine transport activity in suckling rats compared to that in adult animals although direct relationship of cell differentiation with in vivo aging process needs further verification.

• 한국시뮬레이션학회논문지
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• 제13권1호
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• pp.1-9
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• 2004

In this paper some models for obtaining the distribution of a sojourn time in CDMA cellular systems are proposed. Knowledge on this is essential for reliable modeling of the soft handoff and for solving other related problems in the analysis of cellular system. The proposed model is based on random walk and can be adopted to different conditions. Analytical results can be obtained that lead to a quite complicated numerical scheme so simulation models are used for Monte Carlo experiments. Main assumptions include different kinds of mobile carriers (pedestrians and transport passengers) and round shape of a cell. The scheme for simulation experiments is presented along with the discussion of simulation results.

• Mycobiology
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• 제43권3호
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• pp.179-183
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• 2015

Zinc is an essential micronutrient required for many enzymes that play essential roles in a cell. It was estimated that approximately 3% of the total cellular proteins are required for zinc for their functions. Zinc has long been considered as one of the key players in host-pathogen interactions. The host sequesters intracellular zinc by utilizing multiple cellular zinc importers and exporters as a means of nutritional immunity. To overcome extreme zinc limitation within the host environment, pathogenic microbes have successfully evolved a number of mechanisms to secure sufficient concentrations of zinc for their survival and pathogenesis. In this review, we briefly discuss the zinc uptake systems and their regulation in the model fungus Saccharomyces cerevisiae and in major human pathogenic fungi such as Aspergillus fumigatus, Candida albicans, and Cryptococcus gattii.