• Structural Engineering and Mechanics
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• v.71 no.3
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• pp.283-290
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• 2019

In global competition manufacturing companies have to produce modern, new constructions from advanced materials in order to increase competitiveness. The aim of my research was to develop a new composite cellular plate structure, which can be primarily used for structural elements of road, rail, water and air transport vehicles (e.g. vehicle bodies, ship floors). The new structure is novel and innovative, because all materials of the components of the newly developed structure are composites (laminated Carbon Fiber Reinforced Plastic (CFRP) deck plates with pultruded Glass Fiber Reinforced Plastic (GFRP) stiffeners), furthermore combines the characteristics of sandwich and cellular plate structures. The material of the structure is much more advantageous than traditional steel materials, due mainly to its low density, resulting in weight savings, causing lower fuel consumption and less environmental damage. In the study the optimal construction of a given geometry of a structural element of a road truck trailer body was defined by single- and multi-objective optimization (minimal cost and weight). During the single-objective optimization the Flexible Tolerance Optimization method, while during the multi-objective optimization the Particle Swarm Optimization method were used. Seven design constraints were considered: maximum deflection of the structure, buckling of the composite plates, buckling of the stiffeners, stress in the composite plates, stress in the stiffeners, eigenfrequency of the structure, size constraint for design variables. It was confirmed that the developed structure can be used principally as structural elements of transport vehicles and unit load devices (containers) and can be applied also in building construction.

• Journal of Surface Science and Engineering
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• v.44 no.5
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• pp.213-219
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• 2011

Recently aspheric lenses are widely used for superpricision optical instruments, such as cellular phone camera modules, digital cameras and optical communication modules. The aspherical lenses are processed using mold core under high temperature compressive forming pressure. It is imperative to develop superhard protective films for the life extension of lens forming mold core. Especially ta-C films with higher $sp^3$ fractions receive attentions for the life extension of lens forming mold and, in turn, the cost reduction of lenses due to their suprior high temperature stability, high hardness and smooth surfaces. In this study ta-C films were processed on WC mold as a function of substrate bias voltage using FVA (Filtered Vacuum Arc) method. The processed films were characterized by Raman spectroscopy and nano-indentation to investigate bonding nature and hardness, respectively. The film with maximun 87% of $sp^3$ fraction was obtained at the substrate bias voltage of -60 V, which was closest to ta-C film. ta-C films showed better high temperature stability by sustaining relatively high fraction of $sp^3$ bonding even after 2,000 glass lens forming applications.

• Journal of Periodontal and Implant Science
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• v.29 no.1
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• pp.173-192
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• 1999

The purpose of this study was to evaluate the effects of bioactive glass and natural coral on the human periodontal ligament fibroblast(HPLF) behaviors during the regeneration process of peridontium. To determine the cellular events occuring in the presence of the particles of bioactive glass and natural coral, HPLF were isolated from healthy premolar teeth extracted for orthodontic treatment. Cells were cultured in ${\alpha}$MEM at 37$^{\circ}C$, 5% $CO_2$, 95% humidity incubator. Bioactive glass and natural coral were powdered, and each particles(<40${\mu}$m) were placed on the cultured cells at the concentration of 0.3mg/ml, and 1,0mg/ml for experimental group. In control group no particles were added. And each group was evaluated by examining the cell morphology under phase-contrast micrograph at 4 day and transmission electron micrograph(TEM) and scanning electron micrograph(SEM) at 14 day, alkaline phosphatase activity at 5 and 9 day, protain synthesis at 4 day, DNA synthesis at 1, 2, 3 and 4 day, cell proliferation at 1, 3, 5,7 and 9 day and the formation of bone nodule at 30 day after culturing all groups in mineralizing supplemented mediun, No significant changes in cell morphology by adding these two matirials were found under phase contrast microscopy and TEM. HPLF phagocytocized each particles suggesting that HPLF is involved in the process of resorbing each particles and that bioactive glass were more biocompatible than natural coral. The ALPase activity of bioactive glass 0.3 mg/ml was similar with control groups and all the rests of control groups were significantly low(P<0.01) indicating a transient dedifferentiation of HPLF in the presence of bioactive glass and natural coral particles. There were no significant differences of protein synthesis between all groups. The DNA synthesis in experimental groups were significantly lower than control groups at 1, 2 and 3 day (P<0.01) but became similar to control groups at 4 day. Between control groups, the DNA synthesis in bioactive glass O.3mglml group was significantly higher than other groups(P<0.01). Cell proliferation in natural coral 1.0mg/ml and bioactive glass 1.0mglml groups were significantly lower than control group at 3 day(P<0.05) and there were no differences at 5, 7, 9 day. There were more bone nodule formation in experimental groups than in control groups. In conclusion, these results indicated that bioactive glass and natural coral have some effects of a transient dedifferentiation on HPLF and regeneration of periodontal tissues, however any significant cytotoxic effect on HPLF by these two particles were not found.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2003.05a
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• pp.35-38
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• 2003

The purpose of this study is to investigate the manufacture of light weight concrete panel using the artificial light-weight aggregate as a part of the substitution of foamed styrene and polyurethane because of narrow allocable temperature Bone in use. The experimental parameter of this study is 40, 60 and 8$0^{\circ}C$ of curing temperature at 100% relative humidity and the type of admixture such as cement, 6mm glass fiber and St/BA emulsion. Testing item is compressive and flexural strength and strength of specimen cured at standard condition is compared to that of specimen cured at 40, 60 and 8$0^{\circ}C$ of curing temperature at 100% relative humidity. As a result or this, it was revealed that the maximum or strength is developed in 6$0^{\circ}C$ or cure temperature at 100% relative humidity in case of the most of the specimen. Specimens modified by St/BA emulsion show the highest development of strength dependent on the curing tmeperature. So, it seems to be effective that evaporation curing method shoud be considered to curing the specimen as the panel core.

• Journal of Bio-Environment Control
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• v.11 no.1
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• pp.29-34
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• 2002

An experiment was conducted to evaluate shattered industrial polyurethane (PUR) and cellular glass foam (CGF) as growth medium components. Pot chrysanthemum 'Pink Pixie Time 'was cuttured in media containing various volume ratios of PUR, CGF, peatmoss, coir, and perlite. Before plant culture, pH and EC of media were determined. Container capacity (%) was low in perlite and CGF-containing media, but it increased when incorporation ratio of peatmoss or coir was increased. pH was stable between 5.0 and 6.3, but was high in coir-contaning media as compared to other media tested. EC was very high in coir-containing media. Hight at 34 days after planting was the greatest in media containing CGF, and number. of leaves was similar among treatments. Growth in PUR-containinly media was poorer than that in the other media, with some leaf edge burning. Trends in growth measured at 97 days after planting was similar to that measured at 34 days after planting.

• Journal of Bio-Environment Control
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• v.11 no.3
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• pp.108-114
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• 2002

Cellular glass foam (CGE), the reprocessed glass, has a possibility as a component of vegetative propagation media of floricultural crops due to the its excellent air and water permeability, similar to that of perlite. An experiment was conducted to evaluate the rooting and growth thereafter of Kalanchoe blossfeldiana ‘Gold Strike’in media containing various volume ratios of granular rockwool, peat-moss, CGF and perlite. The particle size of CGF and perlite was 2.0~4.0mm and 1.2~4.0mm, respectively. Cuttings were rooted in a fog tunnel with a mean temperature of 18.2$^{\circ}C$ and RH of 66.7％ under a long day regime (14 h per day light period). Height, length of the longest root, stem diameter, no. of leaves, leaf area, percentage of rooted cuttings, shoot and root fresh weights, shoot and root dry weights, total chlorophyll concentration and physicochemical properties were measured. Cuttings rooted 100% in all treatments. Physicochemical properties in CGF and perlite-containing media showed little differences. The growth of rooted plants in the CGF-containing media was similar or rather superior to that in perlite-containing media. Consequently, CGF has a possibility as a vegetative propagation medium of Kalanchoe. To make wider commercial use of CGF, more demonstrative experiments and analyses are necessary.

• Restorative Dentistry and Endodontics
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• v.34 no.3
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• pp.192-198
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• 2009

This study was carried out in order to determine in vitro biocompatibility of white mineral trioxide aggregate (MTA), and to compare it with that of the commonly used materials, i. e. calcium hydroxide liner (Dycal), glass ionomer cement (GIC), and Portland cement which has a similar composition of MTA. To assess the biocompatibility of each material, cytotoxicity was examined using MG-63 cells. The degree of cytotoxicity was evaluated by scanning electron microscopy (SEM) and a colorimetric method, based on reduction of the tetrazolium salt 2,3 bis {2methoxy 4nitro 5[(sulfenylamino) carbonyl] 2H tetrazolium hydroxide} (XTT) assay. The results of SEM revealed the cells in contact with GIC, MTA. and Portland cement at 1 and 3 days were apparently healthy. In contrast, cells in the presence of Dycal appeared rounded and detached. In XTT assay, the cellular activities of the cells incubated with all the test materials except Dycal were similar, which corresponded with the SEM observation. The present study supports the view that MTA is a very biocompatible root perforation repair material. It also suggests that cellular response of Portland cement and GIC are very similar to that of MTA.

• ALGAE
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• v.28 no.2
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• pp.193-202
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• 2013

Nitrogen availability and cell density each affects growth and cellular astaxanthin content of Haematococcus pluvialis, but possible combined effects of these two factors on the content and productivity of astaxanthin, especially under outdoor culture conditions, is less understood. In this study, the effects of the initial biomass densities IBDs of 0.1, 0.5, 0.8, 1.5, 2.7, 3.5, and 5.0 g $L^{-1}$ DW and initial nitrogen concentrations of 0, 4.4, 8.8, and 17.6 mM nitrate on growth and cellular astaxanthin content of H. pluvialis Flotow K-0084 were investigated in outdoor glass column photobioreactors in a batch culture mode. A low IBD of 0.1 g $L^{-1}$ DW led to photo-bleaching of the culture within 1-2 days. When the IBD was 0.5 g $L^{-1}$ and above, the rate at which the increase in biomass density and the astaxanthin content on a per cell basis was higher at lower IBD. When the IBD was optimal (i.e., 0.8 g $L^{-1}$), the maximum astaxanthin content of 3.8% of DW was obtained in the absence of nitrogen, whereas the maximum astaxanthin productivity of 16.0 mg $L^{-1}\;d^{-1}$ was obtained in the same IBD culture containing 4.4 mM nitrogen. The strategies for achieving maximum Haematococcus biomass productivity and for maximum cellular astaxanthin content are discussed.

• Restorative Dentistry and Endodontics
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• v.35 no.5
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• pp.359-367
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• 2010

Objectives: The purpose of the present in vitro study was to evaluate the biocompatibility of mineral trioxide aggregate (MTA) mixed with glass ionomer cement (GIC), and to compare it with that of MTA, GIC, IRM and SuperEBA. Materials and Methods: Experimental groups were divided into 3 groups such as 1 : 1, 2 : 1, and 1 : 2 groups depending on the mixing ratios of MTA powder and GIC powder. Instead of distilled water, GIC liquid was mixed with the powder. This study was carried out using MG-63 cells derived from human osteosarcoma. They were incubated for 1 day on the surfaces of disc samples and examined by scanning electron microscopy. To evaluate the cytotoxicity of test materials quantitatively, XTT assay was used. The cells were exposed to the extracts and incubated. Cell viability was recorded by measuring the optical density of each test well in reference to controls. Results: The SEM revealed that elongated, dense, and almost confluent cells were observed in the cultures of MTA mixed with GIC, MTA and GIC. On the contrary, cells on the surface of IRM or SuperEBA were round in shape. In XTT assay, cell viability of MTA mixed with GIC group was similar to that of MTA or GIC at all time points. IRM and SuperEBA showed significantly lower cell viability than other groups at all time points (p < 0.05). Conclusions: In this research MTA mixed with GIC showed similar cellular responses as MTA and GIC. It suggests that MTA mixed with GIC has good biocompatibility like MTA and GIC.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2010.06a
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• pp.280-280
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• 2010

Alzheimer disease is a progressive neurodegenerative disease of the aged, characterized by memory loss and dementia. For diagnosis of Alzheimer disease we have simply modified macrophage with amyloid beta bonded with different molecules. Modified Macrophage was observed with microscope for co-localization of amyloid beta molecule. For this experiment we used fluoroscene labeling substances. The macrophage was modified also with cell staining method. For cell staining method was used avidin-biotin reaction principles. All experiments were carried out on poly-L-lysine coated and sterilized glass substrates. In the presentation we will show the further investigations and applications with modified macrophage.