Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
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COMPARISON OF BIOCOMPATIBILITY OF FOUR ROOT PERFORATION REPAIR MATERIALS

치근 천공 치료 재료의 생체친화성의 비교

  • Kang, Min-Kyung (Dept. of Conservative Dentistry, School of Dentistry, Chonnam National University) ;
  • Bae, In-Ho (Dept. of Pharmacology and Dental Therapeutics, School of Dentistry, Chonnam National University) ;
  • Koh, Jeong-Tae (Dept. of Pharmacology and Dental Therapeutics, School of Dentistry, Chonnam National University) ;
  • Hwang, Yun-Chan (Dept. of Conservative Dentistry, School of Dentistry, Chonnam National University) ;
  • Hwang, In-Nam (Dept. of Conservative Dentistry, School of Dentistry, Chonnam National University) ;
  • Oh, Won-Mann (Dept. of Conservative Dentistry, School of Dentistry, Chonnam National University)
  • 강민경 (전남대학교 치의학 전문대학원 보존학교실) ;
  • 배인호 (전남대학교 치의학 전문대학원 약리학 교실) ;
  • 고정태 (전남대학교 치의학 전문대학원 약리학 교실) ;
  • 황윤찬 (전남대학교 치의학 전문대학원 보존학교실) ;
  • 황인남 (전남대학교 치의학 전문대학원 보존학교실) ;
  • 오원만 (전남대학교 치의학 전문대학원 보존학교실)
  • Published : 2009.05.31
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Abstract

This study was carried out in order to determine in vitro biocompatibility of white mineral trioxide aggregate (MTA), and to compare it with that of the commonly used materials, i. e. calcium hydroxide liner (Dycal), glass ionomer cement (GIC), and Portland cement which has a similar composition of MTA. To assess the biocompatibility of each material, cytotoxicity was examined using MG-63 cells. The degree of cytotoxicity was evaluated by scanning electron microscopy (SEM) and a colorimetric method, based on reduction of the tetrazolium salt 2,3 bis {2methoxy 4nitro 5[(sulfenylamino) carbonyl] 2H tetrazolium hydroxide} (XTT) assay. The results of SEM revealed the cells in contact with GIC, MTA. and Portland cement at 1 and 3 days were apparently healthy. In contrast, cells in the presence of Dycal appeared rounded and detached. In XTT assay, the cellular activities of the cells incubated with all the test materials except Dycal were similar, which corresponded with the SEM observation. The present study supports the view that MTA is a very biocompatible root perforation repair material. It also suggests that cellular response of Portland cement and GIC are very similar to that of MTA.

이번 연구는 치근 천공의 치료 재료인 white mineral trioxide aggregate (MTA)를 흔히 사용되는 calcium hydroxide liner ($Dycal^{(R)}$), glass ionomer cement (GIC), 그리고 MTA와 유사한 성분을 가진 Portland cement와 세포독성 실험으로 생체 친화성을 평가하는 것이다. 세포독성의 정도는 MG-63 세포를 이용해 주사전자 현미경적 관찰과 수용성 tetrazolium salt를 이용한 흡광도를 측정 (XTT assay)하여 평가하였다. SEM 관찰에서, 1일과 3일째 모두에서 GIC와 MTA, Portland cement 표면에서는 잘 부착된 세포를 보여주었다. 반면에, Dycal 표면의 세포들은 둥글고 부착되지 않은 양상을 보여 주었다. XTT assay에서는 Dycal을 제외한 모든 재료에서 유사하게 높은 세포 활성도를 보여주었으며, 이는 SEM 관찰 소견과 일치하였다. 이번 연구는 MTA가 생체친화적인 재료라는 견해를 뒷받침한다. 또한 Portland cement와 GIC에서도 MTA와 유사한 세포반응을 보여주었다.

Keywords

References

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