• 한국균학회지
• /
• 제17권2호
• /
• pp.82-90
• /
• 1989

각종 균에 대한 침강항체 양성율 (1) 조사대상자 285명 중 104명(36.49%)이 각종 진균에 대한 침강항체를 가지고 있었다. (2) 진균종별로는 침강항체 양성자 104명 중 두 균종 이상의 항원에 대한 양성자를 포함하여 A. fumigatus에 대해 64명 (61.54%) C. albicans에 대해 49명 (47.12%)이었고 A. flavus와 A. niger 및 A. nidlans는 $1{\sim}3$명으로 극소수였다. (3) 성별양성율 빈도는 남성의 경우 A. fumigatus가 24.26% C. albicans가 12.43%인데 비해 여성의 경우 A. fumigatus가 19.83%, C. albicans가 24.14%로서 남성의 경우는 A. fumigatus에 대한 항체가가, 여성의 경우는 C. albicans에 대한 항체가가 높게 나타나 상호 유의성이 있었다(P<0.05). (4) 연령별 양성율의 분포는 $40{\sim}49$세 연령군이 49.12%로서 제일 높았고 $50{\sim}59$세 연령군(43.33%), $30{\sim}39$세 연령군(34.09%), 60세 이상 연령군(31.11%), $20{\sim}29$세 연령군(26.00%) 순이었다. 형광항체반응에 의한 항체검출 (1) 균배양시간에 따른 균체표면 항원의 감수성 간접 형광항체반응으로 조사한 결과 배양시간에 따른 차이점은 관찰할 수 없었다. (2) Aspergillosis 환자의 혈청내에 존재하는 A. fumigatus 항체는 IgG가 가장 많았으므로 간접형광항체반응을 이용한 Aspergillosis 진단시 FITC-labeled anti-lgG를 사용하여야 될 것으로 생각된다. (3) Aspergillosis 속의 균사체 표면항원은 간접 형광항체 반응을 이용하여 항체를 정출시 균종간에 교차반응이 심해서 교차반응 항체를 제거한 후 사용하여 야 될 것으로 사료된다. (4) Aspergillosis 진단에 사용되는 면역학적인방법 중 면역확산법 특이성이 높은데 비해 감수성이 낮고 간접 형광항체반응법은 감수성은 높은데 비해 특이성이 낮으므로 검사목적에 따라 2가지방법을 적절히 병용하는 것이 좋다고 생각된다.

• Radiation Oncology Journal
• /
• 제22권4호
• /
• pp.288-297
• /
• 2004

목적: 종양 내에서 산소공급 부족현상으로 발생하는 저산소증 조직에서 저온온열치료($42^{circ}C$)와 nicotinamide에 의한 perfusion limited 저산소증의 개선 효과를 마우스 종양 모델을 이용하여 종양 내 $[^18F]FMISO$ 섭취변화를 이용하여 증명할 수 있는지 알아보고자 하였다. 대상 및 방법: C3H 마우스에 $[^18F]FMISO$를 정주하고 11개 장기에서 $\%ID/g$을 구하여 biodistribution을 관찰하였다. 또한 같은 마우스에 동종 종양세포인 SCC7을 이식하여 종양모델을 만들고 저온온열치료($42^{circ}C$)와 nico-tinamide를 투여한 마우스와 대조군 마우스에서 $[^18F]FMISO$의 섭취정도 차이를 $\%ID/g$, autoradiography, PET scan을 시행하여 비교하고자 하였다. 결과: 대조군에서 종양의 FMISO의 섭취는 5.1+/-2.28 $\%ID/g$였고, 종양/근육, 종양/혈액의 섭취비는 2.2와 1.8이었다. 실험군에서는 각각 2.4+/-0.64 $\%ID/g$, 1.4와 1.2를 나타내어 대조군보다 유의하게 낮았다(p<0.021). Autoradiography에서 대조군의 종양 내부에 FMISO가 섭취됨을 확인하였고, 저온온열치료와 nico-tinamide를 투여한 실험군에서는 섭취가 감소된 것을 관찰하였다. 결론: C3H 마우스와 동종 종양세포인 SCC-VII을 이용한 종양모델에서 $[^18F]FMISO$가 종양내에 섭취가 되어 자산소증 종양모델로 적절함을 확인하였고, 저온온열치료($42^{circ}C$)와 nicotinamide에 의한 perfusion limited 저산소증 개선효과를 $[^18F]FMISO$의 종양 내 섭취가 감소하는 것을 통하여 확인할 수 있었다.

• 한국신재생에너지학회:학술대회논문집
• /
• 한국신재생에너지학회 2006년도 추계학술대회
• /
• pp.368-371
• /
• 2006

[ $La_{0.8}Sr_{0.2}Co_{1-x}Mn_xO_3$ ] cathode as a high performance cathode on YSZ electrolyte was studied by analyzing impedance spectra. It was shown that cathode property of $La_{0.8}Sr_{0.2}Co_{1-x}Mn_xO_3$ is bet ter than that of$La_{0.8}Sr_{0.2}CoO_3$. At $700^{\circ}C$ in air environment, $La_{0.8}Sr_{0.2}Co_{0.4}Mn_{0.6}O_3$ cathode on CGO- layered YSZ electrolyte showed very low area specific resistance of $0.14{\Omega}cm^2$, which is low enough for intermediate-temperature sol id oxide fuel cells. This is because material properties of ionic conductivity and thermal expansion compatibility with electrolyte were optimized. Judging from activation energy and oxygen part i al pressure dependance of cathode property, it was noted that oxygen surface exchange kinetics is dominantly influential on cathode property in higher temperature region than $700^{\circ}C$ and oxygen self-diffusion in cathode material is more influential in lower temperature region.

• 한국콘텐츠학회논문지
• /
• 제3권3호
• /
• pp.74-84
• /
• 2003

단말기인 UE의 지리적 위치를 제공해 주는 위치 서비스(Location Service： LCS)는 IMT-2000 시스템의 중요한 이슈가 되고 있다. 본 논문에서는 기존 LCS 방식인 셀 영역기반, OTDOA, GPS를 모두 수용하는 하이브리드 방식의 위치측정 방식을 제안하고 이를 위한 IMT-2000 시스템에서 새로운 위치 서비스 시스템의 구조와 동작을 설계한 후, 통신시스템 명세 및 설계언어인 SDL과 SDL개발환경을 이용하여 LCS 프로토콜을 개발한다. 본 프로토콜 설계는 LCS 기능 모델 구조와 시그널링 동작, 시스템 구조, 프리미트브와 데이터 구조 그리고 SDL을 이용한 프로세스 설계 등을 포함한다.

• Nuclear Engineering and Technology
• /
• 제48권1호
• /
• pp.200-210
• /
• 2016

The neutron radiography reactor (NRAD) is a 250 kW Mark-II Training, Research, Isotopes, General Atomics (TRIGA) reactor at Idaho National Laboratory, Idaho Falls, ID, USA. The East Radiography Station (ERS) is one of two neutron beams at the NRAD used for neutron radiography, which sits beneath a large hot cell and is primarily used for neutron radiography of highly radioactive objects. Additional fuel elements were added to the NRAD core in 2013 to increase the excess reactivity of the reactor, and may have changed some characteristics of the neutron beamline. This report discusses characterization of the neutron beamline following the addition of fuel to the NRAD. This work includes determination of the facility category according to the American Society for Testing and Materials (ASTM) standards, and also uses an array of gold foils to determine the neutron beam flux and evaluate the neutron beam profile. The NRAD ERS neutron beam is a Category I neutron radiography facility, the highest possible quality level according to the ASTM. Gold foil activation experiments show that the average neutron flux with length-to-diameter ratio (L/D) = 125 is $5.96{\times}10^6n/cm^2/s$ with a $2{\sigma}$ standard error of $2.90{\times}10^5n/cm^2/s$. The neutron beam profile can be considered flat for qualitative neutron radiographic evaluation purposes. However, the neutron beam profile should be taken into account for quantitative evaluation.

• Journal of Species Research
• /
• 제10권3호
• /
• pp.201-216
• /
• 2021

During an investigation of indigenous prokaryotic species in the Republic of Korea, a total of 38 bacterial strains belonging to the class Gammaproteobacteria were isolated from diverse environments. Samples were collected from soil, seawater, sand, sedimentary soil, rabbit feces, rat intestines, marine wetland, and tidal flats. The strains were identified to the species level using the high 16S rRNA gene sequences and showed high similarity (>98.7%) with the closest bacterial species and formed a robust clade in the neighbor-joining phylogenetic tree; it was determined that each strain belonged to independent, predefined bacteria species within the class Gammaproteobacteria. The 38 strains of Gammaproteobacteria analyzed in this study have not been reported in the Republic of Korea. Therefore, this study describes 20 genera of 13 families in 8 orders: Aeromonadales, Alteromonadales, Cellvibrionales, Enterobacterales, Lysobacterales, Oceanospirillales, Pseudomonadales, and Vibrionales. For each species, we describe Gram reaction, strain ID, isolation source, colony and cell morphology, cultural, physiological, and basic biochemical characteristics.

• Journal of Species Research
• /
• 제10권1호
• /
• pp.12-22
• /
• 2021

During a comprehensive investigation of indigenous prokaryotic species in Korea, 25 and 10 bacterial strains assigned to the classes Alphaproteobacteria and Betaproteobacteria, respectively, were isolated from diverse environmental habitats, including soil, mud, tidal field, sea water, sand, rusted iron, and leaf. Based on their high 16S rRNA gene sequence similarities (>98.7%) and the formation of robust phylogenetic clades with type species, each strain was assigned to an independent and predefined bacterial species. Since there were no published or official reports regarding these 35 isolates in Korea, they - 25 species of 14 families in the 5 orders of Alphaproteobacteria and 10 species of 3 families in the two orders of Betaproteobacteria - have been reported as unrecorded species in Korea. In addition, Gram reaction, colony and cell morphology, basic biochemical characteristic, isolation source, and strain ID of each species are also described in the species description sections.

• 통합자연과학논문집
• /
• 제15권3호
• /
• pp.123-129
• /
• 2022

Schwann cells play a critical role for myelination in peripheral nerve system. It also plays an important role in nerve protection and regeneration. In peripheral nerve damage, regeneration is induced by the migration and proliferation of Schwann cells which were promoted by suppressing the oxidative stress. In this study, Human placental extract was prepared by homogenization and estimated its efficacy in RSC96 cells. Placental extract exhibited a protective effect against hydrogen peroxide-induced oxidative stress in RSC96 cells, confirmed by MTT assay. Furthermore, placental extract decreased intracellular ROS against oxidative stress, confirmed by DCFH-DA assay. Autophagy was visualized with Cyto-ID staining to confirm the autophagy activity of placental extracts. The activity of autophagy was confirmed by immunoblot analysis of autophagy flux-associated proteins such as LC3 conversion and SQSTM1 degradation. Thus, we confirmed the antioxidant effect of placental extract to protect RSC96 cells from oxidative stress, and observed that it activated autophagy and restored autophagy flux.

• Journal of Species Research
• /
• 제12권spc2호
• /
• pp.15-22
• /
• 2023

During a comprehensive investigation of indigenous prokaryotic species in Korea, 20 and one bacterial strains assigned to the phyla Bacillota and Verrucomicrobiota, respectively, were isolated from diverse environmental habitats, including soil, mud, tidal flat, seawater, sand, sediment, brackish water, and healthy human urine. Based on their high 16S rRNA gene sequence similarities (>98.7%) and the formation of robust phylogenetic clades with their closest related reported species, each strain was assigned to an independent and predefined bacterial species. Since there were no published or official reports regarding these 21 isolates in Korea, they - 20 species of four families in two orders of the phylum Bacillota and one species of the phylum Verrucomicrobiota - have been reported as unrecorded species in Korea. In addition, Gram staining, colony and cell morphology, basic biochemical characteristic, isolation source, and strain ID of each species are also described in the species description sections.

• Journal of Species Research
• /
• 제12권spc2호
• /
• pp.23-32
• /
• 2023

During screening for indigenous prokaryotic species in Republic of Korea in 2021, a total of 21 bacterial strains assigned to the phylum Bacteroidota were isolated from a variety of environmental habitats including pine cone, seaweed, soil, sea sediment, brackish water and moss. Based on the 16S rRNA gene sequence similarity value of more than 98.7% and formation of a robust phylogenetic clade with the type strain of the closest bacterial species, it was found that the 21 strains belong to independent and recognized bacterial species. There has been no official report that the identified 21 species have been isolated in Republic of Korea up to date. Therefore, 16 species in six genera of two families in the order Flavobacteriales, two species in two genera of two families in the order Cytophagales, one species in one genus of one family in the order Chitinophagales and two species in one genus of one family in the order Sphingobacteriales are proposed as unrecorded species of the phylum Bacteroidota isolated in Republic of Korea. Their Gram reaction, colony and cell morphology, basic phenotypic characteristics, isolation source, taxonomic status, strain ID and other information are described in the species descriptions.