• 한국축산식품학회:학술대회논문집
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• 한국축산식품학회 2004년도 정기총회 및 제33차 춘계 학술대회
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• pp.318-321
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• 2004

분리동정한 유산균의 cell wall은 murine macrophage를 활성화 시켜 NO와 cytokine을 생성함을 확인하였다. 그 결과 NO 생성량에 있어서는 L. casei SGU 0020에서 가장 높은 분비를 유도하였으며, TNF-a의 분비량은 S. thermophilus SGU 0021의 균주에서 가장 높았다. 그러나 cytokine인 일종인 $IL-1{\beta}$, IL-2, IL-6에 있어서는 L. casei SGU 0020에서 가장 많은 IL의 분비를 유도하였다.

• Journal of Microbiology
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• 제33권4호
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• pp.278-282
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• 1995

S. epidermidis cell was fractionated into cell wall, cell membrane and cytoplasm. The cell membrane adsorbed the most abundant $\textrm{Pb}_{2+}$ per unit dry weight of the three fractions tested. Adsorption behavior of $\textrm{Pb}_{2+}$ in lipid and protein, which are the main components of the cell membrane, indicated that phosphatidylethanolamine and phosphatidylinositol having phosphoryl group and gangliosides containing carboxyl groups adsorbed much more $\textrm{Pb}_{2+}$ than triglycerides lacking any chargeable functional groups. Protein purified from cell membrane adsorbed larger amount of $\textrm{Pb}_{2+}$ than total native cell membrane or cell membrane lipid.

• The Plant Pathology Journal
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• 제32권4호
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• pp.311-320
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• 2016

Xanthomonas axonopodis pv. glycines (Xag) is a necrotrophic bacterial pathogen of the soybean that causes bacterial pustules and is a nonhost pathogen of the chili pepper. In the current study, chili pepper fruit wound inoculated in planta with Xag 8ra formed necrotic lesions on the fruit surface and induced several structural and chemical barriers systemically in the fruit tissue. The initial defense response included programmed cell death of necrotizing and necrotized cells, which was characterized by nuclear DNA cleavage, as detected by TUNEL-confocal laser scanning microscopy (CLSM), and phosphatidylserine exposure on cell walls distal to the infection site, as detected by Annexin V FLUOS-CLSM. These two responses may facilitate cell killing and enhance transportation of cell wall materials used for cell wall thickening, respectively. The cells beneath the necrotic tissue were enlarged and divided to form periclinal cell walls, resulting in extensive formation of several parallel boundary layers at the later stages of infection, accompanying the deposition of wall fortification materials for strengthening structural defenses. These results suggest that nonhost resistance of chili pepper fruit against the nonhost necrotrophic pathogen Xag 8ra is activated systematically from the initial infection until termination of the infection cycle, resulting in complete inhibition of bacterial pathogenesis by utilizing organspecific in situ physiological events governed by the expression of genes in the plant fruit organ.

• 한국환경과학회지
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• 제9권4호
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• pp.331-337
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• 2000

The variation of microorganisms (activated slude, Saccharomyces cerevisiae, Aureobasidium pullulans) caused by the biosorption of $Pb^{2+}$ was observed by TEM and microscope. By the TEM observation of S. cerevisiae, the plasmolysis and lysis of cell wall or cell membrane were occurred by the penetration of $Pb^{2+}$ into the inner cellular region. However, in the case of A. pullulans, the plasmolysis and lysis of cell wall or cell membrane were not occurred because of the prevention of $Pb^{2+}$ penetration by the extracelluar polymeric substances (EPS). A flocculation of microorganisms, in the case of A. pullulans, was observed by the $Pb^{2+}$ accumulation after 3~4 h and the color was changed from white to black after 1 day. The flocculation of activated sludge was improved by the accumulation $Pb^{2+}$ after 1 h, however, the floc was broken up and the settling efficiency decreased after 1 day.

• 한국미생물·생명공학회지
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• 제24권2호
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• pp.143-148
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• 1996

Thousand actinomycetes and 50 soil samples were used for the isolation of microorganisms producing yeast cell wall lytic enzymes. Among 493 strains producing large clear zones on autolysed washed yeast (AWY), 117 strains were selected on living yeast cell agar plates. With the method of lytic activity, one strain (St-1702) was selected, which was temporarily identified as Streptomyces eurythermus. The optimal condition for enzyme production of this strain was partially determined as follows: incubation of the strain for 3 days at 30$\circ$C in the medium containing 2% freeze dried yeast cell, 1% glucose, 1% K$_{2}$HPO$_{4}$, 0.01% MgSO$_{4}$'7H$_{2}$O, 0.5% peptone, and 0.2% (NH$_{4}$)$_{2}$CO$_{3}$ with pH 7.0. The protoplast formation of yeast by using the enzyme produced by this strain was compared with commercial enzymes.

• The Plant Pathology Journal
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• 제20권1호
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• pp.18-21
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• 2004

The root hair provides a major entering spot for the symbiotic legume rhizobia. It is obvious that dynamic cell wall modification occurs in the plant root hair during the early microbe invasion. Expansins are nondestructive cell wall-modifying proteins that are involved in cell growth and differentiation. Among about 40 expansin genes in Arabidopsis, two expansin genes are expressed specifically in the root hair cell. Orthologous genes of this Arabidopsis root hair expansins have been found in other Brassica members, rice, and Medicago truncatula (a legume). In this review, I discuss the probable function of expansins during the early symbiotic process between the root hair and microbes and the regulation of root hair expansin genes in a comparative approach.

• 한국식품과학회지
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• 제18권2호
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• pp.158-162
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• 1986

감의 연화에 따른 세포벽다당류의 조성과 함량 및 gel여과를 통한 개략적인 분자량의 변화를 조사하였다. 조세포벽의 함량은 연화된 감에서 현저히 감소되었는데 구체적으로 ionically associated pectin (IAP )은 59%, covalently bounded pectin (CBP)은 60%, $0{\sim}3N$ KOH soluble hemicellulosic fraction($HF_2$)은 74%가 감소된 반면 4N KOH soluble hemicellulosic fraction($HF_2$)과 cellulosic fraction(CF)은 큰 변화가 없었다. 신선한 감과 연화된 감의 IAP와 CBP 구성다당류의 조성은 uronic acid외에 hemicellulose에 유래한 Pentose와 hexose가 함유되어 있었고 연화에 따라 IAP에서는 pentose가 CBP에서는 hexose가 감소되었다. 개략적인 분자량은 IAP의 400만과 25만의 Peak이 130만과 6만으로, CBP의 경우는 400만이상의 Peak이 60만과 20만으로 CBP에서의 저분자화 정도가 현저하였다. $HF_1$과 $HF_2$ 역시 polyuronide의 함유율이 높았는데, $HF_2$가 현저하였다. 또 연화에 따라 $HF_1$은 pentose가 $HF_2는$ uronic acid와 hexose의 손실율이 높았다. $HF_1$의 연화에 따른 재략적인 분자량은 200만의 peak이 2개로 분리 되었고 $HF_2$는 수백만 단위의 거대분자가 만단위까지 저분자화 하였다. 이상의 결과에서 감의 연화는 CBP에 인결된 hemiceldlose중 특히 $HF_2$의 저분자화와 CBP자체의 분해 내지가용화 현상과 밀접한 관련이 있는 것으로 판단되었다.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 2011년 춘계학술대회논문집
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• pp.2-5
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• 2011

This paper presents a numerical study on multiphase flows induced by wall adhesion The CSF(Continuum Surface Force} model is used for the calculation of the surface tension force and implemented in an in-house solution code(PowerCFD). The present method(code) employs an unstructured cell-centered method based on a conservative pressure-based finite-volume method with volume capturing method(CICSAM) in a volume of fluid(VOF) scheme for phase interface capturing As an application of the present method, the effects of wall adhesion are numerically simulated with the CSF model for a shallow pool of water located at the bottom of a cylindrical tank. Two different cases are computed, one in which the water wets the wall and one in which the water does not wet the wall. It is found that the present method simulates efficiently and accurately surface tension-dominant multiphase flows induced by wall adhesion.

• 대한기관식도과학회지
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• 제4권1호
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• pp.105-111
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• 1998

Double primary cancer is a rare disease in which two cancers occur in an individual independently. As prolonged survival of patients with malignant tumors is expected in the future due to advances in methods of treatment, the chance of double primary cancer will be increased. We experienced one case of double primary cancer which was developed in esophagus and chest wall. A 72 year-old male visited our hospital complaining of epigastric discomfort and right chest wall mass. We studied esophagus, chest wall, and other organs including gastrointestinal tract by various methods to exclude the cancer of other sites and could diagnose squamous cell carcinoma of mid-esophagus and adenocarcinoma of chest wall. The patient underwent esophagogastrostomy following esophagectomy and wide-resection of chest wall tumor. The postoperative course was uneventful.

• 한국미생물·생명공학회지
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• 제17권4호
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• pp.313-320
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• 1989

세포내와 세포벽에 invertase가 존재하며 catabolite inhibition을 받지않고 세포외로 구성적으로 invertase를 생산하는 Rh. glutinis K-24를 공시균주로 선택하여 세포내와 세포벽 invertase를 정제한 후 정제효소의 효소화학적 성질을 밝혔다. 세포내 invertase는 DEAE-Sephadex A-50에 의한 Batchment, DEAE-Sephadex A-50 column chromatography, gel filtration on Sephadex G-200, isoelectric focusing 등의 조작에 의해 Disc 전기영동상 단일한 효소단백질까지 정제되었다. 세포벽 invertase는 B. pumilis에 의해 생산되는 세포벽 용해효소를 작용시켜 invertase를 용출 시킨 다음 DEAE-Sephadex A-50 column chromatography, gel filtration on Sephadex G-100의 조작에 의해 부분 정제하였다. 세포내와 세포벽 Invertase의 분자량은 각각 약 310,000과 67,000이었고 세포내 경우는 subunit 분자량이 약 70,000이었다. 세포내와 세포벽 효소의 반응 최적 pH와 온도는 모두 4.0와 6$0^{\circ}C$였으며 온도 안정성도 7$0^{\circ}C$까지 거의 비슷하게 나타났다. 그러나 Km값은 세포내 효소의 경우는 4.3x$10^{-3}$M이었고 세포벽 효소의 경우는 2.1x$10^{-2}$M이었다. 그외 Inhibitor에 대한 효소의 저해양식도 양효소에 비슷한 결과를 나타내었다.