• Journal of Korean Society of Environmental Engineers
• /
• v.32 no.9
• /
• pp.894-899
• /
• 2010

We coordinated the experiments with ozone pretreatment and two-phase anaerobic digestion using solid-liquid separation to raise the efficiency of sludge volume reduction and obtained the following results. The pre-treatment with ozone reduced the solid concentration in the average of TSS $8.3{\pm}2.0%$ TSS and $9.2{\pm}}2.8%$ VSS. Of the organic material, TCOD decreased $5.1{\pm}2.4%$, but SCOD showed $72{\pm}6.5%$ increased, which was due to destruction of the cell wall and dissolution of icell media by the powerful oxidative stress of ozone. During the two-phase anaerobic digestion process, we achieved the reduction of $21.5{\pm}3.4%$ TSS, $20.2{\pm}8.4%$ VSS, $32.1{\pm}7.9%$ TCOD and $22.1{\pm}7.2%$ SCOD in average. The maximum methane gas production were 177.6 mL per g TSS, 210.8 mL per g VSS, 127.0 mL per g TCOD and 1452.0 mL per g SCOD, respectively. Solid material reduction through the two-phase anaerobic digestion and MLE (Modified Ludzack-Ettinger) processes were 93.8% of TSS and 92.0% of VSS. We concluded that suggested two-phase anaerobic digestion and MLE process could achieve the reasonable production of biogas and a maximum reduction of the sludge volume.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.35 no.4
• /
• pp.342-347
• /
• 2002

Ciliates have the possibility of a new live food in marine finfish culture because of their wide range of body size, thin tell wall, show motility, and fast reproduction rate. In this research, six species of ciliates were isolated from south coast and salt pond in Korea. The fitness of these species as a live food was evaluated in terms of size, motility, suspensibility and cell density. As the result, Euplotes sp. (K-1) was found suitable to be a new live food which might substitute rotifers, Brachionus plintilis and B. rotundiformis in fish larvae culture. The modified $F{\emptyset}yn's$ Erdschreiberd media, MErds-2 with the addition of glycine, glucose and yeast extract increased six times higher growth rate of Euplotes sp. (K-1) than the basic F$\emptyset$yn's Erdschreiberd media. The optimum water temperature, pH and light intensity for this ciliates were $22.5^{\circ}C$, 8 and 2,000 lux, respectively, and its culture environmental range was relatively wide, On the other hand, this ciliate fed baker's yeast, Saccharomyces cererisiae grew up to 1,240 inds./mL with the inocula of 100 inds./mL within 7 days. The results of the study showed that Euplotes sp. (K-1) has a potential to be utilized as a new live food in fish larvae culture.

• Korean Journal of Food Science and Technology
• /
• v.34 no.5
• /
• pp.867-872
• /
• 2002

This study was performed to investigate the optimum process conditions for manufacturing yeast extract from waste brewer's yeast using various enzymes. Contents of IMP, GMP, free amino acids, and crude protein of yeast extracts were measured by enzymes treatment. Crude protein contents of yeast extracts subjected to cell wall digestion enzyme treatment were 21.1, 33.6, and 28.0% for the control grouup, glucanase (0.5%, 12 h), and tunicase (1%, 18 h), respectively. Crude protein contents of yeast extracts subjected to protease treatment were 22.0, 30.8, and 29.8% for control group, bromelin (1%, 3 h), and protamex (1%, 3 h), respectively. Crude protein content of yeast extract subjected to glucanase and protamex mixed treatment was 34.4%. The total contents of IMP and GMP of yeast extracts subjected to G+P+A (glucanase+phosphodiesterase+adenyldeminase) and G+Pro+P+A (glucanase+protamex+phosphodiesterase+adenyldeaminase) treatments were 1,066 and 1,047 mg/100 g, respectively. The content of free amino acids of yeast extract was the highest (2,302 mg/100 g) in G+Pro+P+A treatment. Optimum concentration and process condition of enzyme treatment to obtain yeast extract with high IMP, GMP, and free amino acid content were in the order of glucanase (0.5%, 12 h), protamex (1%, 3h), phosphodiesterase (0.1%, 3 h) and adenyldeaminase (1%, 1.5 h) treatments.

• Korean Journal of Plant Taxonomy
• /
• v.40 no.4
• /
• pp.226-233
• /
• 2010

An intensive study of the embryology of Gymnospermium microrrhynchum was conducted to provide information regarding a discussion of the phylogenetic relationships of the genus, which is yet unstudied. Our results indicated that Gymnospermium is similar to other genera of Berberidaceae in terms of its embryological features. Nevertheless, newly reported and unique features are the well-developed endothelium and the undifferentiated seed coat type. Until the study of Gymnospermium, it may have been considered to be closer to Caulophyllum and Leontice in the tribe Leonticeae. These three genera share many morphological features as well as molecular similarities, by which they are kept in the same tribe, Leonticeae. However, very little detailed embryological data regarding these genera have been published thus far. Gymnospermium was characterized according to the basic type of anther wall formation as well as its glandular tapetum, successive cytokinesis in the microspore mother cell, two-celled mature pollen grains, anatropous and crassinucellate ovules with a nucellar cap, well-developed endothelium, its Polygonum type of embryo sac formation, its nuclear type of endosperm formation, and its undifferentiated seed coat type. In comparison with Nandina, there are many differences, such as the dehiscence of the anther, the cytokinesis in the microspore mother cells, the shape of the megaspore dyad, and the seed characteristics. Although we had no available detailed embryological information regarding Caulophyllum and Leontice, which are genera that are more closely related to Gymnospermium, we could deduce from the phylogenetic relationship that Gymnospermium, Caulophyllum, and Leontice are more closely related to each other than other genera of Berberidaceae on the basis of the seed characteristics.

• Journal of Haehwa Medicine
• /
• v.4 no.2
• /
• pp.335-336
• /
• 1996

Artemisinin, a new antimalarial to treat patients infected with strains of Plasmodium jalciparum, derived from the plant Artemisia annua Linn, has immunopharmacologic actions such as enhence the PHA -induced lymphocyte transformation rate, increased the weight of spleen but reduced the weight of thymus, reduced phagocytic function of peritoneal macrophage, remarkably reduced the level of serum IgG and hemolysin fonning capacity (sentitized with SRBC), inhibited the activity of Ts cells of donor mice by supraoptimal immunuization(SOI), but enhenced activity of Ts cells of recipient mice by SOI. These results suggested that Ts cells may be the target cells of artemisinin. To the serum complement C3 level of plasmodium berghei-infeted mice, artemisinin (i. m,) could remarkly increase it. The artemisinin also obviously reduced the prostaglandin E(PGE) in the mouse hind paw swelling induced by carrageenin. Numerous studies have demonstrated that pharmacologic doses of PGE attenuate the development of immunocomplex nephritis. Some autologous immune mechanisms may be invoolved In the pathogensis of some types of glomurulonephritis. Glomerular abnormalities can be induced in animals by variety of immunological manipulations. The resulting disorder has many clinical and pathogical similarities to the disease in human. Our purpose was therefore to test the ability of the artemisinin to lessen the severity of rabbit IgG accelerated nephrotoxic serum glomerulonephritis in mice model. Mice which had treated with rabbit IgG and NTS, administrated with saline, showed Significant inceases of urinary protein, cholesterol level, and decrease of serum albumin in NS group. On the contrary, By i.g. adminstration of artemisinin at dose of 12.5, 25 and 50 mg/kg for 14 days after NTS injection, shown that artemisinin inhibited the nephritic changes in some parameters by means of urinary protein(p<0.05, p<0.01) and serum choleterol(p<0.05, p<0.01) and albumin (p<0.05, p<0.01), blood urea nitrogen (p<0.05, p<0.01), serum albumin(p<0.05, p<0.01); Cyclophosphamide(i.p. 10mg/kg for 14d) had almost same effect as the artemisinin had. Morphological studies shown that The picture of kidney from the mouse with NTS-nephritis accerated with rabbit IgG, treated with i.g. saline as the control, the mesangiocapillary were enlarged and proliferated; There were inflammatory cells infiltrating around the glomeruli; The ethelial cell were proliferated in the wall of Bowman's capsule. Histopatholological picture of kidney from the NTS-nephritis accerated with rabbit IgG mouse treated with i.p. 10mg/kg cyclophosphamide as the positive control. No siginicant histopathological evidence were found. Treaded with i.p. 12.5mg/kg artemisinine, the picture shown that mesangiocapillary were lightly proliferated; There were inflammatory cells infiltrating around the glomeruli; Treaded with i.p. 25mg/kg artemisinine, The picture shown that the mesangiocapillary were lightly proliferated; Treaded with i.p. 50mg/kg artemisinine, The picture shown that both the mesangiocapillary proliferated and the inflammatory cells infiltrating around the glomeruli are less than treated with saline, 12.5 and 25 mg/kg artemisinine. On the basis of these studies we conclude that the artemisinin can relieve pathological change caused by NTS-nephritis aacerated with rabbit IgG.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.34 no.2
• /
• pp.306-310
• /
• 2005

The comparison in release rate constant and properties of extracts from extruded raw ginseng and extruded white ginseng was conducted to apply extrusion process for manufacturing of released ginseng tea bag. Dry raw ginseng and white ginseng powder were extruded at 20∼30% moisture content and 200∼300 rpm by using an experimental twin-screw extruder. Browness and redness (both indicated the releasing of saponin and ginsenosides) were increased with the increase in the screw speed and the decrease of moisture content. Crude saponin and water solubility index (WSI) of both ginseng also share the same behaviour against the level of screw speed and moisture content, as well as browness and redness. The particle size effects of extruded raw ginseng at 20% and 28% moisture content on absorbance of released extract at 260 up to 560 nm, WSI, and water absorption index were determined. While particle size decreased from 800∼1000 nm to 200∼500 nm, absorbance and WSI are decreased. Absorbance and WSI shown increasing level while moisture content was decreased. In conclusion, the formation of pores by expansion and disruption of cell wall in extrusion cooking were obviously responsible to increase the amount of released extract of extruded ginseng and its WSI as well. The extrusion process turns out be the efficient process for manufacturing of commercial ginseng tea product than those of other thermal processes.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.29 no.3
• /
• pp.371-375
• /
• 2002

The calcifying odontogenic cyst(COC) showed diverse terminology or classification, clinicopathologic features as well as in its biologic behavior, although it was recognized as a distinct clinicopathologic entity. The epithelial lining of a COC appears to have the ability to induce the formation of dental tissues in the adjacent connective tissue wall, and that other odontogenic tumors may sometimes be associated with it. This case is a COC associated with a complex odontoma involving an impacted left maxillary lateral incisor in a 5-year-old female child. Radiographic examination revealed a well-demarcated radiolucent lesion partially occupied by a radiopaque mass, involving the left lateral incisor crown. The histologic sections showed a cystic cavity lined with ameloblastic epithelium containing ghost cell masses with admixed with complex odontoma components. The presence of mixed radiolucent-radiopaque lesion in children as observed in this case, the possibility of COC must be considered. In this case, there was no recurrence 1 year after enucleation and the space control is ongoing now.

• Journal of the East Asian Society of Dietary Life
• /
• v.17 no.3
• /
• pp.402-408
• /
• 2007

This study was conducted in order to evaluate the antioxidant activity of extruded ginseng in different extracted fractions. Each of the fractions obtained from extruded ginseng and ginseng (control) were extracted with 80% ethanol, and then the lipophilic components were removed with ether while the hydrophilic components were separated with water-saturated butanol. Each of the 80% ethanol/butanol/water layers were collected and evaporated to acquire samples for tests of saponin content and antioxidant activity. The antioxidant activity of extruded ginseng fractions and ginseng fractions were determined via the oxygen radical absorbance capacity (ORAC) assay. Overall, the extruded ginseng samples harbored saponin contents of 2.2 (Rg1), 2.3 (Re), 1.2 (Rc), 1.3 (Rb2), and 2.2 (Rd) times that measured in the ginseng prior to extrusion. Antioxidant capacity was also higher, not only in the 80% ethanol/butanol which harbor a significant quantity of saponin, but also in the water fractions, which harbor relatively low quantities of saponin as compared to the control samples. All three of the fractions extracted from extruded ginseng evidence significantly higher antioxidant capacity than the controls (0.05

• Korean Chemical Engineering Research
• /
• v.56 no.2
• /
• pp.229-239
• /
• 2018

The purpose of this study was to effectively produce the biosugar from cell wall of lipid extracted microalgae (LEA) by using microwave-assisted pretreatment without enzymatic hydrolysis process. Response surface methodology (RSM) was applied to optimization of microwave-assisted pretreatment conditions for the production of biosugar based on enzyme-free process from LEA. Microwave power (198~702 W), extraction time (39~241 sec), and sulfuric acid (0~1.0 mol) were used as independent variables for central composite design (CCD) in order to predict optimum pretreatment conditions. It was noted that the pretreatment variables that affect the production of glucose (C6) and xylose (C5) significantly have been identified as the microwave power and extraction time. Additionally, the increase in microwave power and time had led to an increase in biosugar production. The superimposed contour plot for maximizing dependent variables showed the maximum C6 (hexose) and C5 (pentose) yields of 92.7 and 74.5% were estimated by the predicted model under pretreatment condition of 700 w, 185.7 sec, and 0.48 mol, and the yields of C6 and C5 were confirmed as 94.2 and 71.8% by experimental validation, respectively. This study showed that microwave-assisted pretreatment under low temperature below $100^{\circ}C$ with short pretreatment time was verified to be an effective enzyme free pretreatment process for the production of biosugar from LEA compared to conventional pretreatment methods.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.38 no.4
• /
• pp.486-489
• /
• 2009

This study was performed to evaluate contents and estimated availabilities of major minerals (Ca, P, K, Mg) in soybean sprouts. Two different sampling parts (cotyledon and hypocotyl), and three culture periods (1, 3, and 5 days) were evaluated. Results indicated that Mg (99.11%) from cotyledon for 5 days culture period was significantly higher than Ca (98.34%), P (97.67%), and K (98.70%). Percent average Mg estimated availability of 5 days cultured hypocotyl was also significantly higher than that of Ca (77.79%), P (91.91%), and K (96.80%). Therefore, the current results indicate that decreased Ca estimated availability in hypocotyl was time dependent and the cotyledon may be the better source for the estimated availability of major minerals than the hypocotyl of soybean sprouts because more than 90% of the major minerals was estimated availability.