• Title/Summary/Keyword: Cell number

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Electrode Design for Electrode Formation and PV Module Integration Development (전극형성과 태양전지 모듈 일체화 기술 개발에 적용되는 태양전지 전극 설계 기술)

  • Park, Jinjoo;Jeon, Youngwoo;Jang, Minkyu;Kim, Minje;Lim, Donggun
    • Current Photovoltaic Research
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    • v.9 no.4
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    • pp.123-127
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    • 2021
  • This study was on electrode design for the realization of a solar cell that combines electrode formation and module integration process to overcome printing limitations. We used the passivated emitter rear contact (PERC) solar cell. Wafer size was 156.75 mm ×156.75 mm. The fabricated cell results showed that the open-circuit voltage of 649 mV, short-circuit current density of 36.15 mA/cm2, fill factor of 68.5%, and efficiency of 16.06% with electrode conditions the 24BBs with the width 190 ㎛ and 90FBs with the width 45 ㎛. For improving efficiency, the characteristics of the solar cell were checked according to the change in the number of BBs and FBs and the change in line fine width. It is confirmed that the efficiency of the solar cell will be improved by increasing the number of FBs from 90 to 120, and increasing the line width of the FBs by about 10 ㎛ compared to the manufacturing solar cells.

Histopathology of canine basal cell tumor (개에서 발생한 기저세포종양에 관한 병리조직학적 연구)

  • Bak, Eun-jung;Chae, Chan-hee
    • Korean Journal of Veterinary Research
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    • v.35 no.2
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    • pp.361-368
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    • 1995
  • Basal cell tumors from 53 dogs were examined histologically and classified as basal cell adenoma(n=44), granular basal cell adenoma(n=1), basal cell carcinoma(n=3), basosquamous cell adenoma(n=1), basosquamous carcinoma(n=4) on the basis of malignancy and squamous metaplasia. Female was twenty three and male was thirty. Affected dogs are usually 5.6 years and sex predilection have not been seen. None of tumor examined was metastasized into other organs. Distinct patterns identified in the basal cell adenoma are solid(n=15), cystic(n=13), adenoid(n=8), medusa(n=5) and ribbon(n=3). Solid basal cell adenoma is common type in benign basal cell adenoma. Only one neoplasm was granular basal cell adenoma which was characterized by eccentric nucleus and abundant granular cytoplasm. Basal cell carcinoma showed anaplastic appearance histologically and had atypical basaloid epithelial cells and multinucleated giant cells with moderate number of mitotic figures. Both basosquamous cell adenoma and carcinoma had squamous metaplasia.

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The Effect of Electrical Stimulation on Osteoblast Surrounding Dental Implant (치과용 임플란트 주위 뼈모세포에 대한 전기자극의 영향에 관한 연구)

  • Woo, Kyung-Yeup;Kwon, Kung-Rock;Choi, Boo-Byung
    • Journal of Dental Rehabilitation and Applied Science
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    • v.19 no.3
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    • pp.195-206
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    • 2003
  • Several factors can affect the formation of bone tissues surrounding implants. One of the factors is electrical stimulation. It is known to change the movement of cells, form and destroy cells, and alter concentration and chemical component of soft tissues and bones. The effect of electrical stimulation on bone formation can vary according to the intensity of electric currents, stimulating time, the method of sending electric currents, and tissues and cells currents are applied to. This study examines how various enviroments affect osteoblasts. (1) effect on osteoblast with varying intensity of currents Osteoblast-like cells were raised on four plates where implants can be placed. A constant current sink (MC3T3-E1) that can adjust the intensity and stimulating time of electric currents was used. The four plates were stimulated with $0{\mu}A$, $10{\mu}A$, $20{\mu}A$, and $40{\mu}A$, respectively. After 24 hours of stimulation, the number and distribution of cells surrounding implants were examined. (2) effect on osteoblast with varying conditions The 3 study was performed with same method. (1) The change of attached cell number 72-hour after application of various currents (2) The change of attached cell number 72-hour after application of various interval (3) The comparison of attached cell number by implant surface texture The following are the results: 1. The distribution and density of cells surrounding implant is highest under the intensity of electric currents of $20{\mu}A$. 2. The number of cells attached implants is highest under the intensity of electric currents of $20{\mu}A$. 3. The number of cells attached implants is highest under continous electric currents 4. The number of cells attached implants is not different by implant surface texture.

Comparison of Some Tissues in Diploid and Triploid Hybrid between Mud Loach, Misgurnus mizolepis and Cyprinid Loach, M. anguillicaudatus (미꾸라지, Misgurnus mizolepis와 미꾸리, M. anguillicaudatus간 잡종 및 잡종 3배체 일부 조직에서의 세포 및 핵 크기)

  • 박인석;김동수
    • Development and Reproduction
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    • v.4 no.1
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    • pp.19-28
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    • 2000
  • Diploid and triploid hybrid between female mud loach Misgurnus mizolepis and male cyprinid loach, M. andguillicaudatus were produced, using artificial propagation. The influence of hybridization and triploidization on characteristics of the some tissues of retina optic tectum and trunk kidney in loach was determined histologically. Diploid hybrid have almost size similarities in nucleus and the cell size of some tissues to both parents. In the triploid hybrid the nucleus and/or the cell size in erythrocyte, horizontal cell in inner nuclear layer of retina ganglion cell in ganglion cell layer of retina, neuronal cell in granular layer of optic tectum, and secondary proximal tubule cell of trunk kidney, are much larger than those of the corresponding cells in the diploid hybrid individuals. However, triploid hybrid showed fewer number of cells in outer nuclear layer in retina and nuclei in secondary proximal tubule of trunk kidney than in diploid hybrid. Results of this study suggest that some characteristics in the triploid hybrid exhibiting larger cell and nucleus size with fewer number of cell than diploid hybrid can be useful criteria for the distinction between diploid hybrid and triploid hybrid, and the ploidy level in loach.

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Effects of IL-3 and SCF on Histamine Production Kinetics and Cell Phenotype in Rat Bone Marrow-derived Mast Cells

  • Lee, Haneul Nari;Kim, Chul Hwan;Song, Gwan Gyu;Cho, Sung-Weon
    • IMMUNE NETWORK
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    • v.10 no.1
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    • pp.15-25
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    • 2010
  • Background: Rat mast cells were regarded as a good model for mast cell function in immune response. Methods: Rat bone marrow mast cells (BMMC) were prepared both by recombinant rat IL-3 (rrIL-3) and by recombinant mouse stem cell factor (rmSCF), and investigated for both proliferation and differentiation in time course. Rat BMMC was induced by culture of rat bone marrow cells (BMCs) in the presence of both rrIL-3 (5 ng/ml) and rmSCF (5 ng/ml). Culture media were changed 2 times per week with the cell number condition of $5{\times}10^4/ml$ in 6 well plate. Proliferation was analyzed by cell number and cell counting kit-8 (CCK-8) and differentiation was by rat mast cell protease (RMCP) II and histamine. Results: Cell proliferation rates reached a maximum at 8 or 11 days of culture and decreased thereafter. However, both RMCP II production and histamine synthesis peaked after 11 days of culture. By real time RT-PCR, the level of histidine decarboxylase mRNA was more than 500 times higher on culture day 11 than on culture day 5. By transmission electron microscopy, the cells were heterogeneous in size and contained cytoplasmic granules. Using gated flow cytometry, we showed that cultured BMCs expressed high levels of $Fc{\varepsilon}RI$ and the mast cell antigen, ganglioside, on culture day 11. Conclusion: These results indicate that rat BMMCs were generated by culturing BMCs in the presence of rrII-3 and rmSCF and that the BMMCs have the characteristics of mucosal mast cells.

Fabrication and Chracteristics of Cutting Cell with Various Laser Conditions (다양한 레이저 조건에 따른 컷팅셀 제작 및 특성 분석)

  • Park, Jeong Eun;Kim, Dong Sik;Choi, Won Seok;Jang, Jae Joon;Lim, Dong gun
    • Journal of the Korean Solar Energy Society
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    • v.39 no.3
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    • pp.9-17
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    • 2019
  • Laser cutting cell of solar cells can achieve high voltage and efficiency through more array than conventional 6 inch cell compared to same area. In this study, we fabricated c-Si cutting cell with various lasers and laser conditions such as power, speed, and number of times. In the case of picosecond laser, excellent surface characteristics were obtained due to small surface defects and low thermal damage at the output of 20W and the speed of 100 mm/s. However, it is not possible to fabricate a cutting cell having good characteristics due to nonuniform cutting inside the wafer when the processing for forming a cutting cell is not sufficiently performed. For nanosecond lasers, the best wafer characteristics were obtained for fabrication of excellent cutting cells at a frequency of 500 kHz and a laser speed of 100 mm/s. However, the nanosecond laser has not been processed sufficiently in the condition of a number of times. As a result, it was confirmed that the wafer thickness was cut by $63{\mu}m$ of the cell thickness of $170{\mu}m$ in the condition of five times of laser process. It was found that more than 30% of the wafer thickness had to be processed to fabricate the cutting cell. After cutting the 6-inch cell having the voltage of 0.65 V, we obtained the voltage of about 0.63 V.

Intergeneric Somatic Hybrids by Electrofusion of Protoplasts Between Nicotiana tabacum and Petunia inflata (Nicotiana tabacum과 Petunia inflata의 전기적 원형질체융합에 의한 속간 체세포 잡종의 생성)

  • 김준철
    • Journal of Plant Biology
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    • v.30 no.1
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    • pp.1-9
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    • 1987
  • Leaf mesophyll protoplasts of Nicotiana tabacum (nitrate reductase deficient mutant) were fused with cell suspension protoplasts of albino Petunia inflata in an electric field. Hybrid cell colonies were selected for nitrate reductase proficiency and chlorophyll synthesis. Five hybrid plant lines, regenerated from the selected calli lines, were analysed by electrophoresis, number of chromosomes and morphological characters. Somtic hybrid plants showed both parent patterns in the isozymesof isoleucine aminopeptidase and esterase. The hybrids had the expected chromosome number of 62 and exhibited an intermediate floral morphology when compared with the parents, but plant height and leaf arrangement were similar to N. tabacum.

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Effects of Compression Stimulation Application on Cell Proliferation in the Hippocampal Dentate Gyrus of the Sprague-Dawley Rats (흰쥐 해마의 치상회에서 압박자극 적용이 뇌 신경세포 증식에 미치는 영향)

  • Yu, Byong-Kyu;Kim, Kyeong-Mi;Kim, Chang-Ju
    • The Journal of Korean Academy of Sensory Integration
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    • v.2 no.1
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    • pp.21-32
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    • 2004
  • Objective : Effect of treadmill exercise on hippocampal neural cell proliferation under normal conditions and alcohol intoxication conditions has been recently studied; however, this effect under sensory stimulation application has not clarified yet. In the present study, the effect of compression stimulation application on hippocampal neural cell proliferation in the dentate gyrus in normal and alcohol intoxicated rats was investigated. Methods : Experimental design: comparative investigation on number of 5-Bromo-2'-deoxyuridine(BrdU)B-positive cells in dentate gyrus 5 days after commencement. Setting: animal laboratory. Participants: male Sprague-Dawley rats of 3weeks old in age weighing $80{\pm}10gm$. Intervention: animals were randomly assigned into 4 groups; control-rest group(n=8), control-compression group(n=8), alcohol intoxication-rest group(n=8) and alcohol intoxication-compression group(n=8). Animals of the alcohol intoxicated groups were injected intraperitoneally with alcohol(2g/kg) twice per day for 3 days. All animals were injected BrdU(50mg/kg) intraperitoneally, and rats compression stimulation application groups were compressed using sphygmomanometer cuff times per day, for 5 days following alcohol administration. Measures: mean number of BrdU-positive cells in dentate gyrus was observed via immunohistochemistry. Results : Compression stimulation application significantly increased the number of BrdU-positive cells in the dentate gyrus. Also, treatment with alcohol for 3 days inhibited cell proliferation, and compression stimulation application alleviated alcohol-induced inhibition of new cell formation. Conclusion : These results suggest the possibility that compression stimulation application may help in improvement following alcohol-induced brain damaged.

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Suppressive Effect of GJB-7 on Collagen-Induced Arthritis in Mice (관절7호방이 Type II collagen으로 유발된 흰쥐의 관절염에 미치는 영향)

  • Min, Boo-Ki;Oh, Min-Seok
    • The Journal of Korean Medicine
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    • v.31 no.4
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    • pp.63-78
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    • 2010
  • Objectives: This study was carried out to know the effects of Gwan-Jul-Bang-7 (hereafter referred to GJB-7) on the inhibition of arthritis induced by collagen on the mouse. Methods: To assess the effects of GJB-7 on mouse with arthritis induced by collagen II, we conducted several experiments such as analysis of cytotoxicity, hepatotoxicity, arthritis index, total cell number of draining lymph nodes and paw joints, value of immunocyte in PBMC (peripheral blood mononuclear cell), DLN (draining lymph node) and paw joint, measurement of cytokine and anti-collagen II, observation of the histological changes of joint. Results: 1. Cytotoxicity against HFC (human fibroblast cells) was not observed in any concentration and hepatotoxicity was not observed in the GJB-7 treated group. 2. The incidence of arthritis significantly decreased. 3. Total cell number of draining lymph nodes significantly increased and total cell number of paw joints significantly decreased. 4. The percentage of $CD8^+$ cells in PBMC (peripheral blood mononuclear cell) significantly increased. The percentage of $CD3^+/CD69^+$, and $CD3^+/CD49b^+$ cells in PBMC significantly decreased. 5. The percentage of $CD19^+,\;CD3^+$, and $CD4^+/CD25^+$ cells in DLN (draining lymph nodes) significantly increased. The percentage of $B220^+/CD23^+$ cells in DLN significantly decreased. 6. The percentage of $CD3^+,\;CD4^+$, and $CD11b^+/Gr-1^+$ cells in paw joints significantly decreased. 7. The production of TNF-$\alpha$, IL-6, and MCP-1 significantly decreased. 8. Anti-collagen II in serum significantly decreased. 9. With the hematoxylin and eosin stain, inflammation of joint decreased. Under Masson's trichrome stain, the cartilage destruction and synovial cell proliferation and the expression of collagen fibers decreased. Conclusions: Comparison of the results for this study showed that GJB-7 had immunomodulatory effects. So we expect that GJB-7 could be used as an effective drug for not only rheumatoid arthritis but also another auto-immune diseases.

Measurement of Viable Cell Number in Mixed Culture Based on Microbial Respiration Rate (미생물 호흡속도에 기초한 혼합배양중의 생균수 측정)

  • Veljkoic, V.B;;C.R.Engler
    • Microbiology and Biotechnology Letters
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    • v.20 no.6
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    • pp.687-692
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    • 1992
  • A simple method to determine viable cell numbers of each species in mixed culture was developed. The oxygen uptake rate (OUR) equals to the product of the specific OUR and the size of the microbial population. In a mixed culture, the OUR is a result of the respiration activities of each sub-population. The OUR was determined from the slope of the linear relationship between time and the decrease of dissolved oxygen concentration when aeration was stopped. The specific OUR was calculated from the slope of the viable cell number versus OUR curve. These values for C. lusitaniae at 20 and $30^{\circ}C$ were $1.36{\times}10^{-9}$ and $3.90{\times}10^{-9}$ and those for P tannoPhilus at 20 and $30^{\circ}C$ were $0.59{\times}10^{-9}$ and $1.86{\times}10^{-9}$ [(%/s)/(cells/ml)J. respectively. Using these values, viable cell numbers were calculated after the OURs of mixed culture at two temperatures were measured. A good agreement between the viable cell numbers determined by this method and by plate count was obtained.

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