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Suppressive Effect of GJB-7 on Collagen-Induced Arthritis in Mice  

Min, Boo-Ki (Dep. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dae-Jeon University)
Oh, Min-Seok (Dep. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dae-Jeon University)
Publication Information
The Journal of Korean Medicine / v.31, no.4, 2010 , pp. 63-78 More about this Journal
Abstract
Objectives: This study was carried out to know the effects of Gwan-Jul-Bang-7 (hereafter referred to GJB-7) on the inhibition of arthritis induced by collagen on the mouse. Methods: To assess the effects of GJB-7 on mouse with arthritis induced by collagen II, we conducted several experiments such as analysis of cytotoxicity, hepatotoxicity, arthritis index, total cell number of draining lymph nodes and paw joints, value of immunocyte in PBMC (peripheral blood mononuclear cell), DLN (draining lymph node) and paw joint, measurement of cytokine and anti-collagen II, observation of the histological changes of joint. Results: 1. Cytotoxicity against HFC (human fibroblast cells) was not observed in any concentration and hepatotoxicity was not observed in the GJB-7 treated group. 2. The incidence of arthritis significantly decreased. 3. Total cell number of draining lymph nodes significantly increased and total cell number of paw joints significantly decreased. 4. The percentage of $CD8^+$ cells in PBMC (peripheral blood mononuclear cell) significantly increased. The percentage of $CD3^+/CD69^+$, and $CD3^+/CD49b^+$ cells in PBMC significantly decreased. 5. The percentage of $CD19^+,\;CD3^+$, and $CD4^+/CD25^+$ cells in DLN (draining lymph nodes) significantly increased. The percentage of $B220^+/CD23^+$ cells in DLN significantly decreased. 6. The percentage of $CD3^+,\;CD4^+$, and $CD11b^+/Gr-1^+$ cells in paw joints significantly decreased. 7. The production of TNF-$\alpha$, IL-6, and MCP-1 significantly decreased. 8. Anti-collagen II in serum significantly decreased. 9. With the hematoxylin and eosin stain, inflammation of joint decreased. Under Masson's trichrome stain, the cartilage destruction and synovial cell proliferation and the expression of collagen fibers decreased. Conclusions: Comparison of the results for this study showed that GJB-7 had immunomodulatory effects. So we expect that GJB-7 could be used as an effective drug for not only rheumatoid arthritis but also another auto-immune diseases.
Keywords
Gwan-Jul-Bang-7 (GJB-7); rheumatoid arthritis; collagen-induced arthritis; immune cells;
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1 Feldmann M, Maini RN. Anti-TNF alpha therapy of rheumatoid arthritis : what have we learned? Annu Rev Immunol. 2001;19:163-96.   DOI   ScienceOn
2 Cremer MA, Rosloniec EF, Kang AH.. The cartilage collagens: a review of their structure, organization, and role in the pathogenesis of experimental arthritis in animals and in human rheumatic disease. J Mol Med. 1998;76(3-4):275-88.   DOI   ScienceOn
3 Koo BK. Inhibitory Effect of SSKBT on Interleukin-6(IL-6) and Tumor Necrosis Factor-$\alpha$ (TNF-$\alpha$) Induced Inflammatory Cytokine Production in Murine Synovial Cells. Deajeon university graduate school. 2005.
4 Cheung HS. Identification of collagen subtypes in synovial fluid sediments from arthritic patients. Am J Med. 1980;68:73-9.   DOI   ScienceOn
5 Choi EKK. Autoantibodies to type II collagen occurrence in rheumatoid arthritis. autoimmune connective tissue disease and inflammatory syndromes. Ann Rhuem Dis. 1988;47:313-22.   DOI   ScienceOn
6 張仲景. 金匱要略方論. 臺北:大聯國風出版社. 1950:30-9.
7 Trentham DE, Townes AS, Kang AH. Autoimmunity to type II collagen an experimental model of arthritis. J Exp Med. 1977;146(3):857-68.   DOI   ScienceOn
8 張伯臾. 中醫內科學. 人民衛生出版社. 1988:627.
9 金賢濟外. 韓醫學辭典. Seoul:成輔社. 1983:302, 478.
10 Steffen C, Timple R. Antigenicity of collagen and its application in the serological investigation of rheumatoid arthritis area. Int Arch allergy. 1963;22:333-49.   DOI
11 Choi DY, Lee JD, Back YH, Lee SS, Yoo MC, Han JS. Recent Trends of Immunologic Studies of Herbal Medicine on Rheumatoid Arthritis. The Journal of Korean Acupuncture & Moxibustion Society. 2004;21(4):177-96.
12 巢元方. 諸病源候論. 北京. 人民衛生出版社. 1983:51.
13 中國中等醫學敎材編纂委員會. 中醫內科學. 保健新聞社. 1995:682-4.
14 Bae SC. Novel treatment of rheumatoid arthritis. Journal of Korean Academy of Family Medicine. 2001;22(1):21-6.
15 王琦外. 黃帝內經素問今釋. Seoul:成輔社. 1983:206.
16 蔡仁植. 傷寒論譯詮. Seoul:高文社. 1984:9-141.
17 Iwai H, Kozono Y, Hirose S, Akiba H, Yagita H, Okumura K. Amelioration of collagen- induced arthritis by blocade of inducible costimulator-B7 homologous protein costimulation. J of Immunol. 2002;169:4332-9.
18 김동휘 외. 최신진단과 치료. 서울:약업신문사. 1992:634-7.
19 Dolhain, R.J., van der Heiden, A.N., ter Haar. N.T., Breedveld, F.C., Miltenburg, A.M. Shift toward T lymphocytes with a T helper 1 cytokine-secretion profile in the joints of patients with rheumatoid arthritis. Arthritis and Rheumatism. 1996;39:1961-9.   DOI   ScienceOn
20 Brahn. E, Peacock DJ, Banquerigo ML, Liu DY. Effects of tumor necrosis factor alpha (TNF-$\alpha$) on collagen arthritis. Lymphokine Cytokine Res. 1992;11:253.
21 전국 한의과 대학 본초학교수 공편저. 本草學. 서울:영림사. 1995:128, 149, 151, 167, 178, 190, 260, 261, 263, 276, 289, 302, 305, 347, 354, 355, 409, 445, 448, 531, 536, 578, 580.
22 James B Wyngaeden. Cecil Textbook of Medicine. Philadelphia. WA Sauders company. 1992:1508-15.
23 이준희, 김성일. 류마티스 관절염의 역학과 진단. Hanyang medical Reviews. 2005;25(2):4-12.
24 김기현. 痺證. 서울:정담출판사. 1993:23-9.
25 Choi JY, Heo DS, Yoon IJ, Oh MS. Study on the Effect of Gwanjulbang-5 in Rheumatoid Arthritis. Korean Journal of Oriental Physiology and Pathology. 2007;21(3):728-35.   과학기술학회마을
26 Han JS, Song YI, Yoon IJ, Oh MS. Inhibitory Effect of Kuseupbokhap-bang(Quhl fuefang) on Rheumatoid Arthritis in Human Fibroblast-like Synoviocytes. Journal of Oriental Rehabilitation Medicine. 2006;16(3):1-16.
27 Hong SS, Yoon IJ, Oh MS. Study on the Effect of Geopungjeseup-tang in Rheumatoid Arthritis. Korean Journal of Oriental Physiology and Pathology. 2006;20(5):1241-4.   과학기술학회마을
28 Bischoff SC, Krieger M, Brunner T, Dahinden CA (May 1992). Monocyte chemotactic protein 1 is a potent activator of human basophils. J. Exp. Med. 1992;175(5):1271-5.   DOI   ScienceOn
29 해리슨 번역 편찬위원회. 해리슨 내과학. 서울:정담출판사. 2006:78-99.
30 Chin N. Tran, Steven K. Lundy, David A. Fox. Synovial biology and T cell in rheumatoid arthritis. Pathophysiology. 2005;12(3):183-9.   DOI   ScienceOn
31 Hwang DS. Effects of Kagammokbanggi-tang on the Adjuvant-induced arthritis in rats. Semyung university graduate school. 2005.
32 Lee JH. Immunity effect of the Sogyughwalhyeltanggami in collagen-induced arthritis(CIA) mice. Deajeon university graduate school. 2005.
33 Kishimoto T, Akira S, Taga T. Interleukin-6 and its receptor a paradigm for cytokines. Science. Oct1992;258(5082):593-7.   DOI
34 Conti P, Boucher W, Letourneau R, Feliciani C, Reale M, Barbacane RC. Monocyte chemotactic protein-1 provokes mast cell aggregation and 〔3H〕5HT release. Immunology. 1995;86(3):434-40.
35 Kim MK, Oh MS. Inhibitory effects of Gamidaeganghwal-tang(Jiaweidaqinghuo-tang) on rheumatoid arthritis induced by type II Collagen. Journal of Oriental Rehabilitation Medicine. 2009;19(2):89-102.
36 Peter Parham. 면역학 제2판. Seoul:Lifescience. 2006:454.
37 Kim YH. A study on the biological actions and funtions of cytokines. Journal of Jeonju Kijeon University. 2000;20:271-85.
38 대한 정형외과학회. 정형외과학. 서울:최신의학사. 2001:123-31, 175-89.
39 Cho YJ. Effects of Siegesbeckiae herba on pro-inflammatory cytokines and rheumatoid arthritis. Deajeon university graduate school. 2003.
40 세포분자면역학 교재연구회 역. 세포분자면역학(6판). 서울:이퍼블릭. 2008:11-3, 31-4, 145, 147, 148, 150, 172, 173, 441, 510, 531-6.
41 Lim JS. (The) Experimental Study of Gyehyeoldeung-bokhapbang on the Collagen-Induced Arthritis. Semyung university graduate school. 2006.
42 Choi JS. Song TW. A Study on the Effects of Spatholubus suberectus Dunn on the Inhibition of Arthritis Induced by Collagen on the Mouse. Korean Journal of Herbology. 2003;18(3):79-88.   과학기술학회마을