• Korean Journal of Animal Reproduction
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• v.14 no.2
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• pp.133-140
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• 1990

This study was conducted to develop a simple and efficient technique for fusing 2-cell mouse embryos to obtain tertraploid embryos. Various concentration of PEG and exposure times were compared in order to determine the best condition for fusion and subsequent of fused embryos. The results obtained were follows ; 1. The incidence of fusion induction treated with 40% PEG(70.8%) and 45%(62.7%) for 60 sec. exposure were higher than those of 40% and 45% PEG for 30 sec., 90 sec., or 120 sec. exposure group. Also, the highest incidence of fusion induction(76.9%) was achieved with 120 sec. exposure at 50% PEG concentration. 2. Fused embryos after PEG treatment were cleavaged 2-to 4-cell, 8-cell, morula and blastocyst at 20-24 hr., 30-34., 44-52 hr., respectively, and were not different from those obtained fleshly. 3. The high proportions of the embryos developed to blastocysts after blastomere fusion with 40% PEG for 60 sec., 45% PEG for 60 sec. and 50% for 120 sec. were 66.7%(42/63), 69.0%(29/42) and 32.0%(16/50), respectively, this trend indicated that the fusion rate was similar to the incidence of fused embryos forming blastocysts. 4. The cell number of blastocyst developed from fused embryos(18.7 2.6) was samller than that of untreated embryos(48.9 1.69)

• Journal of Plant Biotechnology
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• v.41 no.2
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• pp.65-72
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• 2014

Plant cells from which the cell walls have been enzymatically or mechanically removed are called protoplasts. The protoplasts are theoretically totipotent and can be used as sources of somatic cell fusion in practical breeding programs. Wild Solanum species have often been used as sources of important agricultural traits including diverse disease resistance. However, they cannot often be directly applied to breeding programs due to their sexual incompatibility with S. tuberosum. Somatic hybridization via protoplast fusion is one of the ideal methods to overcome this limitation and to introgress certain traits into S. tuberosum. This technique has still widely been used in potato since the first fusion was reported in 1970s. Therefore, this review highlights general perspectives of protoplast fusion and discusses the application of protoplast fusion in potato breeding.

• Journal of Microbiology and Biotechnology
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• v.2 no.1
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• pp.46-49
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• 1992

The availability of Phaffia rhodozyma as an astaxanthin sources in the aquaculture industry is limited because of the low carotenoid content of natural isolate. In this study, we have used the protoplast fusion technique to construct cell hybrids with an increased content of astaxanthin from P. rhodozyma. Cell hybrids (F307 and F406) obtained were very stable and produced considerably more astaxanthin (> 1 mg/g yeast) than the wild parent. Karyogamy was confirmed by the isolation of recombinants after mitotic segregation of parental auxotrophic genetic markers, the increased amount of chromosomal DNA/cell and the presence of single nucleus/cell.

• Korean Journal of Veterinary Research
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• v.27 no.2
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• pp.259-267
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• 1987

Enterotoxigenic E. coli (ETEC) cause an acute diarrhea (white scour) in both animals and humans. The disease process initially involves the adherence and colonization of the mucosal surface of the small intestine, followed by the elaboration of a heat-labile enterotoxin (LT) and/or heat-stable enterotoxin (ST). Intestinal adherence or colonization by ETEC is generally mediated by a specific surface-associated pilus (fimbrial) antigen that endows the bacteria with the capacity to adhere to epitherial cell surface. Fourteen monoclonal antibodies (MAbs) directed against pili antigens of ETEC were obtained by cell fusion/hybridoma technique. They were characterized by indirect immunofluorescence assay (IFA), and divided into four groups: specific to K99 antigen (group 1), cross-reactive with K99 and F41 antigens (group 2), specific to K88 antigen (group 3) and specific to 987P and K88 antigens (group 4), respectively. These MAbs demonstrated the distinct pili (K) antigens on the surface of ETEC by IFA, and could be utilized as diagnostic reagent for the identification of ETEC. When eighty-seven field isolates of E. coli from piglet with diarrhea were tested by group 3 MAb, fourty-two strains (48.3%) has K88 pilus antigen suggesting that this is one of the major pilus antigen of ETEC present in fifeld.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.9 no.2
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• pp.528-544
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• 2015

Base Station Cooperation (BSC) has been a promising technique for combating the Inter-Cell Interference (ICI) by exchanging information through a high-speed optical fiber back-haul to increase the diversity gain. In this paper, we propose a novel pilot symbol assisted data fusion scheme for distributed Uplink BSC (UBSC) based on Differential Evolution (DE) algorithm. Furthermore, the proposed scheme exploits the pre-defined pilot symbols as the sample of transmitted symbols to constitute a sub-optimal Weight Calculation (WC) model. To circumvent the non-linear programming problem of the proposed sub-optimal model, DE algorithm is employed for searching the proper fusion weights. Compared with the existing equal weights based soft combining scheme, the proposed scheme can adaptively adjust the fusion weights according to the accuracy of cooperative information, which remains the relatively low computational complexity and back-haul traffic. Performance analysis and simulation results show that, the proposed scheme can significantly improve the system performance with the pilot settings of the existing standards.

• Korean Journal of Microbiology
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• v.30 no.5
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• pp.333-338
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• 1992

Saccharomyces cerevisiae contains 10-nm tilament ring which lies just under the inner surface of the plasma membrane within the mother-bud neck. Although H)-nm filaments may he involved in cellular morphogenesis. their role and organization are not clear. Here we report the production of antihodies specific for the CDel2 protein hy use of gene fusion techniques. and studies on the organization and function of IO-nm filaments using these antibodies. The CDCl2 protein arc translated through the whtlle cell cycle and present in the cytosol. 'They are polymerized just before bud emergence and unpolymerized alier cytokinesis. and do not have organizational relationship with actin. Thc possible role of 10-nm filaments is the determination of bud emergence site and completion of cytokinesis.

• Korean Journal of Veterinary Research
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• v.38 no.1
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• pp.209-216
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• 1998

The purpose of present study is to improve the efficiency of fusion and the developmental rates of nuclear transplanted embryos to produce genetically identical twins from Korean native cattle. The diameter of aspirated follicles had no significant effect on the recovery rates of oocytes collected by ovum pick-up technique. The fusion rates of nuclear transplanted embryos were significantly higher in 50 and $100{\mu}s$ DC duration groups(73.3 and 72.0% ; respectively) than that in $30{\mu}s$ group(55.6% ; p<0.05). The cleavage rates of nuclear transplanted embryos appeared to be significantly higher in donor nuclei derived from in vivo (65.0%) than in those from in vitro (50.5% ; p<0.01), but the developmental rates to morulae and blastocysts were not significantly different between them(13.7 vs 10.9%, respectively).

• Journal of the Korean Society for Precision Engineering
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• v.28 no.12
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• pp.1372-1378
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• 2011

The smart air condition system is superior to conventional air condition system in the aspect of control accuracy, environmental preservation and it is foundation for intelligent vehicle such as electric vehicle, fuel cell vehicle. In this paper, failure analyses of smart air condition system will be performed and then sensor fusion technique will be proposed for fail safety of smart air condition system. A sensor fusion logic of air condition system by using CO sensor, $CO_2$ sensor and VOC, $NO_x$ sensor will be developed and simulated by fault injection simulation. The fusion technology of smart air condition system is generated in an experiment and a performance analysis is conducted with fusion algorithms. The proposed algorithm adds the error characteristic of each sensor as a conditional probability value, and ensures greater accuracy by performing the track fusion with the sensors with the most reliable performance.

• Archives of Pharmacal Research
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• v.16 no.4
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• pp.271-275
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• 1993

In order to prepare a novel human insulin analogue suhbstituted with homoserine at B$^{30}$ / position, (B$^{30}$ /-homoserine) human insulin, a synthetic gene was designed by linking directly a gene for B chain with that for A chain. This gene was constructed by enzymatic joining of 10 different synthetic oligonucleotides, and then inserted at the polylinker region of pUC19 plasmid. To achieve a high level of gene expression, the gene fusion technique region of pUC19 plasmid. To achieve a high level of gene expression, the gene fusion technique was employed using amino terminal regions of lacZ gene up to Clal or hpal, and either of them has been located under tac promoter. The chemical induction of these fused genes by isopropyl-.betha.-D-thiogalactopyranoside (IPTG) gave a satisfactory level of expression in Escherichia coli harboring the ocnstructed plasmids. It was observed that the fused gene product as a single chain insulin precusor was produced more than 30% of total cell protein of E. coli as a form of inclusion body.

• KSBB Journal
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• v.5 no.3
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• pp.255-261
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• 1990

Cell fusion between complementary mutants isolated from astaxanthin-producing yeast, Phaffia rhodozyma, was carried out to obtain astaxanthin-overproducing strains by protoplast fusion technique. The frequency of protoplast fusion was ranged from 2.3$\times$10-5 to 6.0$\times$10-5, and nuclear fusion in the cells of hybrids was demonstrated by several techniques such as isolation of recombinants after mitotic segregation of parental genetic markers, estimation of DNA content, direct observation of nuclei with nuclear staining, and comparison of survival rate to UV exposure. One of several hybrids, Fl, showed approximately 3-fold increase in astaxanthin content when compared with wild parent.