• 자연과학논문집
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• 제11권1호
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• pp.55-63
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• 1999

효모세포의 원형질체 최적형성을 위한 stabilizer의 종류 및 농도, pH 그리고 lysis 방법을 조사하는 한편, intact cell과 protoplast사이의 효소활성도 및 poly-P 생합성율을 비교하였다. 그 결과 protoplast 형성에 있어 snail gut enzyme은 5시간, drisielase는 3시간 정도의 incubation 시간이 필요했으며, stabilizer로는 0.8 M mannitol, 6 M KCl이 좋았다. Protoplast는 intact cell에 비해 ALPase 활성은 22-27%, ACPase는 4-15% 정도 감소하였으며, poly-P 형성은 protoplast에서 유의한 증가가 일어나지 않았다.

• 산업경영시스템학회지
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• 제21권45호
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• pp.329-332
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• 1998

This Paper proposes a mathematical model to solve the cell formation problem with exceptional elements, Exceptional elements are bottleneck machines and exceptional parts that span two or more manufacturing cells. The model suggests whether it is cost-effective to eliminate an EE (by machine duplication or part subcontracting), or whether the intercellular transfer caused by the EE should remain in the cell formation. It provides an optimal solution for resolving the interaction created by EE in the initial cell formation solution. In addition, the model recognizes potentially advantageous mixed strategies ignored by previous approaches.

• 한국경영과학회지
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• 제17권2호
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• pp.25-33
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• 1992

Manufacturing cell formation is one of the most important problems faced in designing cellular manufacturing systems. The purpose of this study is to design effective manufacturing cell systems by developing a method which forms machines/parts into optimal machine cells/part families. The 0-1 data matrix structure is used to form a basis for manufacturing cell formation. In this paper, we propose a CE method to reorder the 0-1 data matrix for manufacturing cell formation. The resulting solutions are shown to demonstrate the effectiveness of the CE method.

• Asian-Australasian Journal of Animal Sciences
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• 제22권2호
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• pp.194-200
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• 2009

The aim of this study was to investigate the effects of donor cell passage, size and type on the development of nuclear transfer embryos. Porcine cumulus cells, fetal fibroblasts and oviductal epithelial cells from 1-2, 3-6 and 7-10 passages were used for the nuclear transfer. In the oocytes with the cumulus donor cells, fusion and cleavage rates of oocytes and cell numbers per blastocyst among the three different passage groups did not show any differences, but the rates of blastocyst formation from 1-2 and 3-6 passage groups were higher than those from 7-10 passage group. The rates of fusion, cleavage and blastocyst formation, and the cell numbers per blastocyst were higher in the embryos with the sizes of <20 and 20 ${\mu}m$ cumulus donor cells compared to the >20 ${\mu}m$ cumulus donor cell. In the oocytes with the fetal fibroblast donor cells, the rate of blastocyst formation from the 3-6 passage group was higher than from 1-2 and 7-10 passage groups. The embryos with the size of 20 $\mu{m}$ fetal fibroblast donor cell showed higher rate of blastocyst formation compared to those with <20 and >20 ${\mu}m$ donor cells. In the oocytes with the oviductal epithelial cells, the rates of blastocyst formation from 1-2 and 3-6 passage groups were higher compared to those from 7-10 passage group. The embryos with the sizes of <20 and 20 ${\mu}m$ oviductal epithelial donor cells had a higher rate of blastocyst formation compared to those with >20 ${\mu}m$ donor cell. Fusion and cleavage rates of oocytes, and cell numbers per blastocyst among the three different donor cell types from the 3-6 passage did not show any differences. However, the rate of blastocyst formation of somatic cell nuclear transfer (SCNT) embryos with the fetal fibroblast donor cell was higher than that of blastocyst formation of SCNT embryos with the cumulus and oviductal epithelial donor cells.

• ALGAE
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• 제20권3호
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• pp.239-249
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• 2005

Some marine coenocytic green algae could form protoplasts from the extruded protoplasm in seawater. The dissociated cell components of the coenocytic protoplasm could be reunited into live cells and, hence, the formation of new species by mixing protoplasms from different coenocytic cells has been predicted. Our results showed that an incompatibility barrier was present during protoplast formation in coenocytic algae to exclude foreign inorganic particles or alien cell components. No inorganic particles or alien cell components were incorporated into protoplast formed spontaneously in seawater. Even when the inorganic particles or alien cell and/or cell component were incorporated into protoplast in some experimental condition, they were expelled from the protoplast or degenerated within several days. A species-specific cytotoxicity was observed during protoplast hybridization between the protoplasms of Bryopsis spp. and Microdictyon umbilicatum. The cell sap of M. umbilicatum could destroy the cell components of Bryopsis spp., but had no effect on Chaetomorpha moniligera. Species C. moniligera and Bryopsis did not affect protoplast generation of either species. The wound-induced protoplast formation in vitro might have evolved in some coenocytic algae as a dispersal method, and the incompatibility barrier to alien particles or cell and/or cell component could serve as a protective mechanism for successful propagation.

• 한국동물학회지
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• 제30권3호
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• pp.248-260
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• 1987

In order to investigate the importance of the prospective mesodermal and endodermal blastomeres at 32-cell stage in the anis formation, blastomeres were deleted or transplanted into the ventrovegital site of another normal embryo. The results are as follows: When the dorsomesodermal or dorsoendodermal blastomeres were deleted, there was a substantial developmental lesion in the axis structure. However, when the ventromesodermal or ventroendodermal blastomeres were deleted, the formation of an axis structure was nearly normal. The dorsomesodermal or dorsoendodermal blastomeres which were transplanted into the ventral side of the normal 32-cell embryo caused the formation of a secondary body axis, and the capacity of the second axis induction in the dorsomesodermal blastomeres was a little higher than that in the dorsoendodermal blastomeres. These results imply that both the dorsomesodermal and dorsoendodermal blastomeres are involved in the formation of a set of dorsal body structures during early embryogenesis. As well, in order to investigate the axis inducing capacity in the early cleavage embryos, the dorsovegital blastomeres were transplanted into the ventrovegital site at 4-cell, 8-cell and 16-ceIL stage respectively. As a ruts·fIt, a second body axis was formed. Therefore, it seems that the early cleavage embryo as 4-cell stage dorsal blastomeres contain some informations necessary for the axis formation.

• 한국컴퓨터정보학회논문지
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• 제21권2호
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• pp.71-77
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• 2016

There has been unknown polynomial time algorithm for cellular formation problem (CFP) that is one of the NP-hard problem. Therefore metaheuristic method has been applied this problem to obtain approximated solution. This paper shows the existence of polynomial-time heuristic algorithm in CFP. The proposed algorithm performs coarse-grained and fine-grained cell formation process. In coarse-grained cell formation process, the cell can be formed in accordance with machine frequently used that is the number of other products use same machine with special product. As a result, the machine can be assigned to most used cell. In fine-grained process, the product and machine are moved into other cell that has a improved grouping efficiency. For 35 experimental data, this heuristic algorithm performs better grouping efficiency for 12 data than best known of meta-heuristic methods.

• 한국경영과학회지
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• 제26권1호
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• pp.71-85
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• 2001

This paper considers a cost model for a U-shaped manufacturing cell formation which incorporates a required number of machines and various material flows together under multi-part multi-cell environment. The model is required to satisfy both the specified operation sequence of each part and the total part demand volume, which are considered to derive material handling cost in U-shaped flow line cells. In the model several cost-incurring factors including set-up for batch change-over, processing time for operations of each part, and machine failures are also considered in association with processing load and capacity of each cell. Moreover, a heuristic for a good machine layout in each cell is newly proposed based on the material handling cost of each alternative sequence layout. These all are put together to present an efficient heuristic for the U-shaped independent-cell formation problem, numerical problems are solved to illustrate the algorithm.

• Journal of Preventive Medicine and Public Health
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• 제30권3호
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• pp.555-566
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• 1997

This study was carried out to evaluate the cytotoxicity of rock wool fibers(RWFs) such as cell division disturbance, chromosomal and DNA damage, and mutagenicity using cultured cells. RWFs were the man made mineral fibers. In order to find the correlation between the cytotoxicity of RWFs and the phagocytic capacity of cells, the phagocytic processes were observed using scanning electron microscope. Cell division disturbance by RWFs was evaluated by the formation of multinucleated giant cells. The chromosomal damage was evaluated by the micronucleus formation. For the evaluation of oxidative DNA damage, 8-hydroxy-2'-deoxyguanosine (8-OH-dG) formation was measured utilizing calf thymus DNA. Mutagenicity was determined by the point mutation of HGPRT and the effect of RWFs on cell transformation was also observed. 1. Compared with the results of chrysotile, RWFs were no or little effect on the cell growth according to the results done by the tests of cell proliferation inhibition and relative plating efficiency. 2. The frequency of multinucleated giant cell formation was increased by the treatment of RWFs and it was dose-dependent. However, the effect of RWFs was weaker than that of chrysotile. 3. The number of micronuclei formed in the RWFs treated cells was between those of cells treated with chrysotile and those of untreated cells. 4. The 2 fold increase in the formation of 8-OH-dG in calf thymus DNA was observed in the cells treated with RWFs in the presence of $H_2O_2$. On the other hand, chrysotile had no effect on the 8-OH-dG formation. 5. RWFs had no effect on the HGPRT point mutation and cell transformation. These results showed that RWFs could induce chromosomal damage, cell division disturbance and oxidative DNA damage in the RWFs treated cells.

• BMB Reports
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• 제45권3호
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• pp.189-193
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• 2012

Cyst formation is a major characteristic of ADPKD and is caused by the abnormal proliferation of epithelial cells. Renal cyst formation disrupts renal function and induces diverse complications. The mechanism of cyst formation is unclear. mIMCD-3 cells were established to develop simple epithelial cell cysts in 3-D culture. We confirmed previously that Mxi1 plays a role in cyst formation in Mxi1-deficient mice. Cysts in Mxi1 transfectanted cells were showed by collagen or mebiol gels in 3-D cell culture system. Causative genes of ADPKD were measured by q RT-PCR. Herein, Mxi1 transfectants rarely formed a simple epithelial cyst and induced cell death. Overexpression of Mxi1 resulted in a decrease in the PKD1, PKD2 and c-myc mRNA relating to the pathway of cyst formation. These data indicate that Mxi1 influences cyst formation of mIMCD-3 cells in 3-D culture and that Mxi1 may control the mechanism of renal cyst formation.