• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2010년도 추계학술대회 논문집
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• pp.729-730
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• 2010

• 대한전자공학회논문지TC
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• 제47권7호
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• pp.1-7
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• 2010

본 논문에서는 16레벨셀 낸드 플래시 메모리 채널에 최대 유사도 검출 방법을 이용하여 데이터를 검출하기 위해 트렐리스의 정답 값을 추정하는 기법에 대해 연구 하였다. 이 기법은 최대유사도 검출기를 사용할 수 있게 되어 성능향상에 도움을 준다. 플래시 메모리는 커플링 효과 때문에 메모리가 있는 채널 모델링이므로, 이미 알고 있는 데이터 열을 훈련 과정을 통해 트렐리스의 정답 값을 추정하여, 이 값을 토대로 최대 유사도 검출한다. 본 실험을 통해 문턱 전압을 이용한 데이터 검출 방법보다 제안한 기법을 이용한 최대 유사도 검출기의 성능이 좋은 것을 보였다.

• 한국동물위생학회지
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• 제25권3호
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• pp.295-297
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• 2002

This experiment was done to set up the immunohistochemical detection method for infectious hematopoietic necrosis virus(IHNV) antigens in the monolayers of CHSE-214 cell cultures inoculated with IHNV. Specific identification of IHNV antigens was detected in the cytoplasms of infected cells by the use of monoclonal antibodies to glycoproteins. The specific positive signal was observed as a distinct red color. The result showed that streptavidin alkaline phosphatase immunohistochemistry specifically identified IHNV antigens in infected cultured cells.

• 한국수소및신에너지학회논문집
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• 제23권4호
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• pp.316-322
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• 2012

A fuel cell vehicle has the hydrogen detection sensors for checking the hydrogen leakage because it use hydrogen for its fuel and can't use a odorant to protect the fuel cell stack. To verify the hydrogen safety of leakage we select the high possible leak points of fittings in hydrogen storage system and test the leaking behavior at them. The hydrogen leakage flow rate is 10, 40, 118 NL/min and the criterion for maximum hydrogen leakage is based on allowing an equivalent release of combustion energy as permitted by gasoline vehicles in FMVSS301. There are total 18EA hydrogen leakage detection sensors installed in test system. we acquire the hydrogen leakage detection time and determine the ranking. Hydrogen leakage detection time decrease when hydrogen leakage flow rate increase. The minimum hydrogen leakage detection time is about 3 seconds when the flow rate is 118NL/min. In this study, we optimize hydrogen sensor position in fuel cell vehicle and verify the hydrogen leakage safety because there is no inflow inside the vehicle.

• KSBB Journal
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• 제29권5호
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• pp.361-371
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• 2014

Mycoplasma is well recognized as one of the most prevalent and serious microbial contaminants of biologic manufacturing processes. Conventional methods for mycoplasma testing, direct culture method and indirect indicator cell culture method, are lengthy, costly and less sensitive to noncultivable species. In this report, we describe a new TaqMan probe-based real-time PCR method for rapid and quantitative detection of mycoplasma contamination during manufacture of biologics. Universal mycoplasma primers were used for mycoplasma PCR and mycoplasma DNA was quantified by use of a specific TaqMan probe. Specificity, sensitivity, and robustness of the real-time PCR method was validated according to the European Pharmacopoeia. The validation results met required criteria to justify its use as a replacement for the culture method. The established real-time PCR assay was successfully applied to the detection of mycoplasma from human keratinocyte and mesenchymal stem cell as well as Vero cell lines artificially infected with mycoplasma. The overall results indicated that this rapid, specific, sensitive, and robust assay can be reliably used for quantitative detection of mycoplasma contamination during manufacture of biologics.

• Asian Pacific Journal of Cancer Prevention
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• 제16권18호
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• pp.8491-8494
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• 2016

Purpose: To compare unsatisfactory rates and detection of abnormal cervical cytology between conventional cytology or Papanicolaou smear (CC) and liquid-based cytology (LBC). Materials and Methods: A total of 23,030 cases of cervical cytology performed at King Chulalongkorn Memorial Hospital during 2012-2013 were reviewed. The percentage unsatisfactory and detection rates of abnormal cytology were compared between CC and LBC methods. Results: There was no difference in unsatisfactory rates between CC and LBC methods (0.1% vs. 0.1%, p = 0.84). The detection rate for squamous cell abnormalities was significantly higher with the LBC method (7.7% vs. 11.5%, p < 0.001), but those for overall abnormal glandular epithelium were similar (0.4% vs. 0.6%, p = 0.13). Low grade squamous lesion (ASC-US and LSIL) were more frequently detected by the LBC method (6.1% vs. 9.5%, p < 0.001). However, there was no difference in high gradd squamous lesions (1.1% vs. 1.1%, p = 0.95). When comparing between types of glandular abnormality, there was no significant difference the groups. Conclusions: There was no difference in unsatisfactory rates between the conventional smear and LBC. However, LBC could detect low grade squamous cell abnormalities more than CC, while there were similar rates of detection of high grade squamous cell lesions and glandular cell abnormalities.

• 융합신호처리학회논문지
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• 제13권4호
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• pp.180-186
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• 2012

본 연구에서는 육각형상을 포함하는 잡음이 많은 저 대비 영상으로부터 에지를 검출하는 방법을 제안한다. 이 방법은 라플라시안-가우시안 필터의 조합과 형상에 의존하는 필터의 아이디어에 기초하고 있다. 먼저, 모퉁이에서 특히 육각형의 에지를 검출하기 위한 검출기로서 6 개의 마스크를 갖는 알고리즘을 사용한다. 여기에서 두 개의 삼각화살 모양의 필터는 육각형의 삼각화살 모양의 접속부를 검출하기 위해 사용되고, 나머지 네 개의 마스크는 육각형 에지의 선성분을 강조하기 위해 사용된다. 자연영상으로서 보통 규칙적인 육각형상의 각막 내피 세포를 선택하며, 이 각막 내피 세포내 육각형상의 에지 검출은 임상 진단에 있어서 중요하다. 그 다음, 에지 검출법의 유효성을 평가하기 위해 제안 알고리즘과 기존 방법을 잡음을 포함하는 육각형 영상에 적용한 결과 본 제안 방법이 다른 방법에 비해 신호 대 잡음비와 에지의 일치율 및 검출 정확도에서 잡음에 대한 강인성과 양호한 검출 능력을 나타낸다.

• Journal of Information Processing Systems
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• 제15권6호
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• pp.1392-1405
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• 2019

The cathode voltage of aluminum electrolytic cell is relatively stable under normal conditions and fluctuates greatly when it has an anomaly. In order to detect the abnormal range of cathode voltage, an anomaly detection algorithm based on sliding window was proposed. The algorithm combines the time series segmentation linear representation method and the k-nearest neighbor local anomaly detection algorithm, which is more efficient than the direct detection of the original sequence. The algorithm first segments the cathode voltage time series, then calculates the length, the slope, and the mean of each line segment pattern, and maps them into a set of spatial objects. And then the local anomaly detection algorithm is used to detect abnormal patterns according to the local anomaly factor and the pattern length. The experimental results showed that the algorithm can effectively detect the abnormal range of cathode voltage.

• Bulletin of the Korean Chemical Society
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• 제33권6호
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• pp.1983-1988
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• 2012

Stem cell transplantation is emerging as a possible new treatment for liver cirrhosis, and recent animal studies have documented the benefits of stem cell therapy in a hepatic fibrosis model. However, the underlying mechanism of stem cell therapy is still unclear. Among the proposed mechanisms, the cell replacement mechanism is the oldest and most important, in which permanently damaged tissue can be replaced by normal tissue to restore function. In the present study, Cy5.5-labeled superparamagnetic iron oxide (SPIO) was used to label human mesenchymal stem cells. The uptake of fluorescently labeled nanoparticles enabled the detection and monitoring of the transplanted stem cells; therefore, we confirmed the direct incorporation and differentiation of SPIO into the hepatocyte-like transplanted stem cells by detecting human tyrosine aminotransferase (TAT), well-known enzymatic marker for hepatocyte-specific differentiation.

• BMB Reports
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• 제55권3호
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• pp.154-159
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• 2022

Protein S-glutathionylation is a reversible post-translational modification on cysteine residues forming a mixed disulfide with glutathione. S-glutathionylation, not only protects proteins from oxidation but also regulates the functions of proteins involved in various cellular signaling pathways. In this study, we developed a method for the detection of S-glutathionylated proteins (ProSSG) using eosin-glutathione (E-GSH) and mouse glutaredoxin 1 (mGrx1). ProSSG was efficiently and specifically labeled with E-GSH to form ProSSG-E via thiol-disulfide exchange. ProSSG-E was readily luminescent allowing the detection of ProSSG with semi-quantitative determination. In addition, a deglutathionylation enzyme mGrx1 specifically released E-GSH from ProSSG-E, which increased fluorescence allowing a sensitive determination of ProSSG levels. Application of the method to the adipocyte differentiation of 3T3-L1 cells showed specific detection of ProSSG and its increase upon differentiation induction, which was consistent with the result obtained by conventional immunoblot analysis, but with greater specificity and sensitivity.