• Korean Journal of Veterinary Research
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• v.57 no.1
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• pp.9-15
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• 2017

To construct a novel vaccine candidate against bovine enterotoxigenic Escherichia coli (ETEC), FanC, the major subunit of K99 fimbriae adhesion, was inserted into secretion plasmid pYA3560 containing a ${\beta}-lactamase$ secretion system. This was then transformed into ${\Delta}asd$ ${\Delta}crp$ Salmonella (S.) Typhimurium and designated as JOL950. Secretion of recombinant fanC fimbrial antigens was confirmed by immunoblot analysis. Groups of mice were inoculated with single or double doses of JOL950. Another group was used as a negative control. Compared to control mice, all immunized mice had significantly higher levels (p < 0.05) of serum immunoglobulin (Ig)G, and secretory IgA against FanC. The IgG2a and IgG1 titer assays revealed that immunization highly induced IgG2a compared to that of IgG1, indicating that T helper-1- related cell-mediated immune responses may be elicited by JOL950. The results show that both systemic and mucosal immunities against selected fimbrial antigens of bovine ETEC expressed by a live attenuated S. Typhimurium strain are prominently produced in mice immunized with JOL950 via an oral route.

• Journal of Pharmaceutical Investigation
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• v.36 no.5
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• pp.293-297
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• 2006

Curcumin is a phytochemical compound with anticancer activity. Although curcumin has substantial pharmacological effect against various cancers, the low solubility of curcumin has hindered its development. For an organic solvent-free injectable formulation, we encapsulated curcumin in various liposomes. Due to its lipophilic property, curcumin was placed in the membrane region of liposomes. Curcumin was stably encapsulated in all formulations tested in this study. The cellular uptake of curcumin delivered in liposomal formulations or free form was measured in K562 human leukemia cell lines using a flow cytometry and MTT viability assay, respectively. Although all the liposomes could solubilize curcumin, the cellular levels and the anticancer effects of liposomal curcumin varied with the composition of liposomes. Moreover, liposomal curcumin down-regulated the expression of Notch-1, the molecule involved in the carcinogenesis, to the similar extent to free curcumin dissolved in dimethyl sulfoxide. These results warrant the development of liposomal curcumin as an injectable formulation for leukemia treatment.

• Journal of Haehwa Medicine
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• v.19 no.1
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• pp.49-54
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• 2010

Inhalational drug is an attractive modality for local therapy of pulmonary diseases as well as systemic drug delivery. Flower of Lonicera japonica (FLJ) and flower of Tussilag farfara (FTF) are medicinal herbs for respiratory disease in traditional Korean medicine. As a preliminary study for effective inhalable formulation of FLJ and FTF, this study was to provide the toxicity and anti-inflammatory effect on murine macrophages. The dried FLJ and FTF were extracted with distilled water, filtered and freeze-dried. After treatment with FLJ and FTF extract on RAW 264.7 cells, the cell viabilities were measured by MTT assay. FLJ and FTF did not show cytotoxicity on RAW 264.7 cells. LPS stimulated RAW 264.7 cells were treated with 3 and $30\;{\mu}g/ml$ of FLJ or FTF. FLJ and FTF did not inhibit TNF-a and IL-6 secretion in both concentration of treatment. We suggest that FLJ and FTF may be useful drugs for respiratory disease. Future work will focus on the physical characteristics for inhalable formulation.

• Journal of Communications and Networks
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• v.17 no.2
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• pp.133-144
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• 2015

In this paper, we introduce a system and a set of algorithms for disseminating popular content to a large group of wireless clients spread over a wide area. This area is partitioned into multiple cells and there is a base station in each cell which is able to broadcast to the clients within its radio coverage. Dissemination of information in the proposed system is hybrid in nature: Each base station broadcasts a fraction of information in the form of random linear combinations of data blocks. Then the clients cooperate by exchanging packets to obtain their desired messages while they are moving arbitrarily over the area. In this paper, fundamental trade-offs between the average information delivery completion time at the clients and different parameters of the system such as bandwidth usage by the base stations, average energy consumption by the clients and the popularity of the spread information are studied. Moreover different heuristic algorithms are proposed to control and maintain a balance over these trade-offs. Also, the more complicated case of multiple sessions where each client is interested in an arbitrary subset of sessions is considered and two variants of the basic dissemination algorithm are proposed. The performance of all the proposed algorithms is evaluated via extensive numerical experiments.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.40 no.3
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• pp.602-612
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• 2015

Current IEEE 802.11p which is suggested for vehicle to vehicle communication supports one hop communication. Thus, it has a limitation to carry out efficient data dissemination. In this thesis, we suggest LTE-D2D based vehicle network to provide efficient data dissemination in the vehicle environment. In this network architecture, we use name based message with IP packet options and we put the intermediate vehicle node called 'super vehicle node' and each normal vehicle node in the cell requests data to the super vehicle node. The super vehicle node responses data to the normal vehicle node. Performance analysis is based mathematical modeling. We compare LTE cellular network to LTE-D2D based vehicle network about throughput according to packet delivery time.

• Journal of Pharmaceutical Investigation
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• v.28 no.1
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• pp.43-50
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• 1998

Niosomes are vesicles formed from synthetic non-ionic surfactants, offering an alternative to chemically unstable and expensive liposomes as a drug carrier. Non-ionic surfactant and cholesterol mixture film leads to the formation of vesicular system by hydration with sonication method. The formation of niosome was ascertained by negative staining of TEM. The entrapment efficiency of niosomal suspension was gradually increased with increasing the ratio of cholesterol to surfactant. It was found that the niosome with 6 : 4 (polyoxyethylene 2-cetyl ether: cholesterol) ratio was more stable than those with other ratios. The topical application of acyclovir(ACV) in the treatment of herpes simplex virus type 1(HSV-1) skin disease has a long history. There are an increasing number of reports, however, in which topical ACV therapy is not as effective as oral administration. Lack of efficacy with topical ACV has been hypothesized to reflect the inadequate delivery of drug to the skin. We investigated the permeation of niosome containing $[^{3}H]ACV$ in hairless mouse skin using Franz diffusion cell model. Permeation coefficient(P) of aqueous ACV was $6.7{\times}10^{-4}\;(cm/hr)$ and that of ACV in niosome was $23.4{\times}10^{-4}\;(cm/hr)$, suggesting about 3.5 times increase in the transdermal permeation.

• Journal of Microbiology and Biotechnology
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• v.27 no.9
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• pp.1657-1663
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• 2017

Cosmetic patches have recently been developed as skin products for personal care owing to rapid advances in the technology of delivery of active ingredients, moisture, and adhesiveness to skin. Alginate and Spirulina are typical marine resources used in cosmetic products. This research involved the development of a Spirulina extract-impregnated alginate nanofiber cosmetic patch supported by a polycaprolactone (PCL) nanofiber cover (Spi/Alg-PCL NF patch). In addition to the ability of alginate to affect moisture and adhesiveness to skin, the impregnation of Spirulina extract strengthened those abilities as well as its own bioactive effectiveness. All fabrication processing steps were undertaken in aqueous solution. The three components (alginate, Spirulina extract, and PCL) had no detected cytotoxicity in human keratinocyte cell-based examination. In addition, wetting the pre-dried patch on the skin resulted in the Spirulina extract being released within 30 min. The results indicate the excellence of the Spi/Alg-PCL NF patch as a skin-care cosmetic device.

• Biomolecules & Therapeutics
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• v.7 no.3
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• pp.263-270
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• 1999

The in vitro permeation of thyrotropin-releasing hormone (TRH) through rabbit nasal, rectal and duodenal mucosae was studied in the absence and presence of an enzyme inhibitor and permeation enhancer. TRH in the donor and receptor solutions was assayed by HPLC. When thimerosal (TM, 0.5 mM) was added to the donor cell as an inhibitor, the permeation rate of TRH (200 $\mu\textrm{g}$/ml) increased linearly as a function of time. Fluxes of TRH through the nasal, rectal and duodenal mucosae were found to be 33.3$\pm$5.9, 11.8$\pm$1.9 and 9.6$\pm$0.7 $\mu\textrm{g}$/$\textrm{Cm}^2$/hr, respectively. The addition of sodium glycocholate, glycyrrhizic acid ammonium salt, sodium taurodihydrofusidate or L-$\alpha$-lysophosphatidylcholine to the donor solution containing TM did not result in the significant increase of permeation flux except for the duodenal mucosa, comparing with that in the presence of TM alone. Consequently, it was suggested that the nasal route was advantageous for systemic delivery of TRH, and the addition of TM and/or an enhancer was necessary to maximize the transmucosal permeation of TRH.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2009.05a
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• pp.281-286
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• 2009

The proposed technique uses cyclic frame structure, where three periods such as beacon period (BP), mesh contention access period (MCAP) and slotted period (SP) are in a data frame. This paper studies on a mechanism to allow communication nodes (6lowpan) in a PAN with different logical channel for global healthcare applications monitoring technology. The proposed super framework structure system has installed 6lowpan sensor nodes to communicate with each other. The basic idea is to time share logical channels to perform 6lowpan sensor node. The concept of 6lowpan sensor node and various biomedical sensors fixed on the patient BAN (Body Area Network) for monitoring health condition. In PAN (hospital area), has fixed gateways that received biomedical data from 6lowpan (patient). Each 6lowpan sensor node (patient) has IP-addresses that would be directly connected to the internet. With the help of IP-address service provider can recognize or analyze patient data from all over the globe by the internet service provider, with specific equipments i.e. cell phone, PDA, note book. The NS-2.33 result shows the performance of data transmission delay and data delivery ratio in the case of hop count in a PAN (Personal Area Networks).

• Journal of Broadcast Engineering
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• v.26 no.5
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• pp.592-598
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• 2021

Current cellular systems rely on a CBS protocol for emergency alert broadcast services. However, the CBS protocol just specifies the delivery of a limited text message. Therefore, foreigners, who are unfamiliar with local characters, may have some difficulties in understanding the received CBS text message. The CBS protocol also reveals a distinct restriction in delivering abundant information because of a limited number of text characters. In order to overcome the weak points of the current CBS protocol, we propose a display method of image information and URL on the screens of mobile terminals for the received CBS text message in this paper. The presented approach effectively utilizes the message structure of CBS for 5G cellular systems.