• 한국의학물리학회지:의학물리
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• 제28권1호
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• pp.11-15
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• 2017

Relative biological effectiveness (RBE) of particle beam needs to be evaluated at particle beam therapy centers before the clinical application of the particle beam. However, since RBE analysis is implemented manually, it is useful to have a tool that can easily and effectively handle the data of experiments to generate cell survival curve and to analyze RBE simultaneously. In this work, the development of a program for RBE analysis of particle beam therapy was presented. This RBE analysis program was developed to include two parts; fitting the cell survival curves to linear-quadratic model and calculating the RBE values at a certain endpoint using fitting results. This program was also developed to simultaneously compare and analyze the template results that stored experiment data with photon and particle beam irradiations. The results of the cell survival curve obtained by each irradiation can be analyzed by the user on a desired data after reading the template stored in the easy-to-use excel file. The analysis results include the cell survival curves with error range, which are appeared in the screen and the ${\alpha}$ and ${\beta}$ parameters of linear-quadratic model with 95% confidence intervals, RBE values, and $R^2$ values to evaluate goodness-of-fit of survival curves to model, which are stored in a text cvs file. This software can generate cell survival curve, fit to model, and calculate RBE all at once with raw experiment data, so it helps users to save time for data handling and to reduce the possibility of making error on analysis. As a coming plan, we will create a user-friendly graphical user interface to present the results more intuitively.

• 지식경영연구
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• 제24권1호
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• pp.127-145
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• 2023

본 논문은 친환경 연료 중 하나인 수소 원료를 바탕으로 미래의 에너지 문제와 공해 문제를 해결할 수 있는 수소연료전지 기술 도메인에 대해 특허 분석을 실시했다. 특허분석은 현재 기술 수준과 미래 발전 방향을 발견할 수 있어 기술로드맵 수립에 많이 활용된다. 그러나 기존의 특허분석은 정성적인 분석 및 간단한 통계분석으로 현재의 기술 환경을 반영하지 않은 분석 결과를 제안할 수 없다. 현재의 기술 환경은 기술의 융복합으로 발전을 나타내고 있고, 발전 속도는 매우 가파르게 성장하여 정성적인 분석은 시대적 분석 요건에 옳지 않다. 그래서 본 논문은 현재의 기술환경을 반영한 지식상속성(Knowledge Persistence, KP) 기반의 주경로 분석과 텍스트마이닝 방법을 활용하여, 수소연로전지 기술도메인에서 핵심특허 발견, 중요 기술 발전, 유망기술을 조망한다.

• Composites Research
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• 제37권1호
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• pp.46-52
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• 2024

운송 수단의 발전은 인간의 교통 편의 증진과 더불어 이동이 불편한 장애인들의 이동 반경 확대를 가능하게 하였다. 그러나 휠체어 탑재 차량의 경우 차량 사고 시 발생할 수 있는 안전성은 일반 승객 좌석에 비해 여전히 낮다. 특히 무방비 상태에서 발생할 수 있는 후방 추돌 사고의 경우 장애인 탑승객의 목 부상에 치명적으로 작용할 수 있다. 따라서 휠체어 탑재 차량에 적용될 headrest에는 보다 세밀한 설계안이 반영되어야 한다. 본 연구에서는 휠체어 운송 차량의 저속 후방 추돌 시 headrest의 국부적 압축 특성 분포 구현을 위해 multi-cell headrest가 제안되었다. 이후 해석을 통한 데이터셋 구축과 k-means clustering을 적용한 군집화 결과를 이용해 탑승객의 목 상해지수와 충격 에너지 흡수량 간 상관관계 분석이 수행되었다. 군집화 결과 유사한 특성을 지닌 데이터 군집이 형성된 것을 확인하였으며, 각 군집의 특성을 통한 목 상해지수와 충격 에너지 흡수량 간의 상관관계 분석이 수행되었다. 분석 결과 Mid3와 Mid6에서의 cell 압축 특성이 soft할수록 충격 에너지 흡수량이 증가하는 것을 확인하였으며, Front2, Mid3, Mid6에서의 cell 압축 특성이 hard할수록 목 상해지수 감소에 효과적임을 확인하였다.

• 한국자기학회:학술대회 개요집
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• 한국자기학회 2003년도 하계학술연구발표회 및 한.일 공동심포지엄
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• pp.174-175
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• 2003

• 전자공학회논문지A
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• 제32A권12호
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• pp.9-24
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• 1995

In this paper, we proposed and analysed two kinds of priority control mechanism to archive the cell loss rate requirement and the delay requirement of each class. The service classes of our concern are the high time priority class(class 1) and the high loss priority class(class 2). Two kinds of priority control mechanism is divided by the method of storing the arriving class 2 cell in buffer on case of buffer full. The first one is the method which discarding the arriving class 2 cell, the second one is the mothod which storing the arriving class 2 cell on behalf of pushing out the class 1 cell in buffer. In the proposed priority schemes, one cell of the class 1 is transmitted whenever the maximum K cells of the class 2 is transmitted on case of transmitting the class 1 cell and the class 2 cell sequentially. In this paper, we analysed the cell loss rate and the mean cell delay for each class of the proposed priority scheme by using the Markov chain. The analytical results show that the characteristic of the mean cell delay becomes better for the class 1 cell and that of the cell loss rate becomes better for the class 2 cell by selecting properly the cell transfer ratio according to the condition of input traffic.

• 대한한방내과학회지
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• 제27권1호
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• pp.149-165
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• 2006

Objectives: This study was done to evaluate the effects of Saengkankunbi-tang on cell-viability, proliferation, cell-cycle, apoptosis and DNA replication on HepG2 cell and to find out by which molecular-biological mechanism by which Saengkankunbi-tang operates. Methods : The MTT assay, cell counting assay, [3H]-thymidine incorporation assay, flow cytometric analysis, tryphan blue exclusion assay, western blot analysis, quantative RT-PCR were taken. Results : Saengkankunbi-tang had no effect on proliferation, cell-cycle and DNA replications of HepG2 cells, while it improved cell viability and reduced apoptosis, and it activated Akt and NFKB. But, it did not produce an effect on cell viability and apoptosis when P13K/Akt pathway was blocked by LY294002 nor when $NF{\kapa}B$ activation was blocked by DN-$I{\kapa}B$. Conclusion : These results suggests that Saengkankunbi-tang improves cell viability and reduces apoptosis of HepG2 cells, by activating $NF{\kapa}B$ through PI3K/Akt pathway.

• 대한한의학회지
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• 제29권1호
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• pp.95-105
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• 2008

Objectives : Although herbal medicines containing flavonoids have been reported to exert anti-tumor activities, it has not been explored whether Hwang-Jeong (Polygonati sibirici Rhizoma, PsR) exerts anti-tumor activity in human glioma. The present study was therefore undertaken to examine the effect of PsR on cell viability and to determine its underlying mechanism in A172 human glioma cells. Methods : Cell viability was estimated by MTT assay. Reactive oxygen species generation and mitochondrial membrane potential were measured by the fluorescence dyes. The phosphorylation of kinases was evaluated by western blot analysis and caspase activity was estimated using colorimetric assay kit. Results : PsR resulted in loss of cell viability in a dose- and time-dependent manner. PsR did not increase reactive oxygen species (ROS) generation and the PsR-induced cell death was also not affected by antioxidants, suggesting that ROS generation is not involved in loss of cell viability. Western blot analysis showed that PsR treatment caused rapid reduction in phosphorylation of extracellular signal-regulated kinase (ERK) without changes in p38 and Jun-NH2-terminal kinase (JNK). U0126, an inhibitor of ERK, increased the PsR-induced cell death, but inhibitors of p38 and JNK did not affect the cell death. PsR induced depolarization of mitochondrial membrane potential. Caspase activity was not stimulated by PsR and caspase inhibitors did not prevent the PsR-induced cell death. Conclusion : Taken together, these findings suggest that PsR results in human glioma cell death through caspaseindependent mechanisms involving down-regulation of ERK.

• Design & Manufacturing
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• 제14권2호
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• pp.1-6
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• 2020

Recently, research and commercialization related to the field of cell-based therapeutic drug development has been actively conducted. In order to maintain cell viability and prevent contamination, refrigeration preservation devices, such as CRF (controlled rate freezer) or vapor type LN2 tanks have been developed. On the other hand, the storage container for liquid nitrogen tanks currently on sale minimizes the flow structure to prevent structural defects when stored in a liquid nitrogen tank having a high thermal conductivity than vapor nitrogen. If the cell-based treatment drug is stored in the gaseous LN2 tank as it is, the cell survival after thawing is greatly reduced. It was estimated that the existing storage container structure was a factor that prevented the rapid entry and circulation of gaseous nitrogen into the container. Therefore, this study intends to propose a new supercellular storage container model that can maintain the mechanical strength while maximizing the fluid flow structure. To this end, we estimated that the structural change of the storage container effects on the equivalent stress formed around the through-holes of them when exposed to a cryogenic environment using thermal-structural coupled field analysis. As a result of storage experiments in the gas phase tank of the cell-based therapeutic agent using the developed storage container, it was confirmed that the cell growth rate was improved from 66% to 77%, which satisfied the transportation standards of the FDA(Food and Drug Administration) cell-based therapeutic agent.

• Advances in Traditional Medicine
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• 제10권4호
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• pp.254-262
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• 2010

Artemisia capillaris (A. capillaries) is known to play roles in many cellular events, such as cell proliferation, differentiation, and apoptosis. We investigated the antifibrogenic efficacy of A. capillaris in the immortalized human hepatic stellate cell line LX2. Cell proliferation was determined by the MTT assay. Cell cycle was analyzed by the flow cytometry. Apoptotic cells were measured using a cell death detection ELISA. Caspase activity was detected by a colorimetric assay. The mRNA level of Bcl-2 and Bax mRNA were measured by real-time PCR. MEK and ERK protein were detected by Western blot analysis. We provide evidence that A. capillaris induces cell cycle arrest, apoptosis, and potently inhibits the mitogen-activated protein kinase pathway. A. capillaris inhibited cell proliferation of LX2 cells in a dose- and time-dependent manner, increased the apoptosis fraction at cell cycle analysis with an accompanying DNA fragmentation, and resulted in a significant decrease in Bcl-2 mRNA levels and an increase in Bax expression. Exposure of LX2 cells to A. capillaris induced caspase-3 activation, but co-treatment of A. capillaris with the pan-caspase inhibitor Z-VAD-FMK, and the caspase-3 inhibitor Z-DEVE-FMK, blocked apoptosis. A. capillaris down-regulated Mcl-1 protein levels and inhibited phosphorylation of MEK/ERK, suggesting that it mediates cell death in LX2 cells through the down-regulation of Mcl-1 protein via a MEK/ERK-independent pathway.

• 한국수소및신에너지학회논문집
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• 제22권2호
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• pp.129-138
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• 2011

Performance changes of an anode-supported tubular SOFC including current collectors are analyzed at different current collecting methods using numerical simulation. From the two dimensional numerical model of the solid oxide fuel cell with nickel felts as anodic current collectors and silver wires as cathodic ones, the performance curves and the distributions of temperature, concentration, current density are obtained. Also, the voltage loss of the cell is divided into three parts: activation loss, concentration loss and ohmic loss. The results show that the performance change of the cell is dominantly influenced by the ohmic loss. Although the temperature and concentration distributions are different, the total activation loss and concentration loss are nearly same. And the ohmic loss is divided into each parts of the cell components. The ohmic loss of the anodic current collectorreaches about 60~80% of the cell's total ohmic loss. Therefore, the reduction of the ohmic loss of the anodic current collector is very important for stack power enhancement. It is also recommended that the load should be connected to the both ends of the anodic current collector.