• 제목/요약/키워드: Cell Extraction

검색결과 865건 처리시간 0.025초

청각 구조를 이용한 잡음 음성의 인식 성능 향상 (Performance Improvement of Speech Recognizer in Noisy Environments Based on Auditory Modeling)

  • 정호영;김도영;은종관;이수영
    • 한국음향학회지
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    • 제14권5호
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    • pp.51-57
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    • 1995
  • 본 논문에서는 청각 모델을 기초로 잡음에 강한 음성 특징 추출을 연구하였다. 청각모델은 basilar membrane 모델, 섬모세포(hair cell) 모델과 스펙트럼 출력단으로 구성하였다. Basilar membrane 모델은 음파의 진동에 따른 전달 특성을 묘사한 것으로 대역 통과 필터의 열로 나타난다. 섬모 세포 모델은 basilar membrane의 진동에 의한 신경 물질로의 변환을 나타낸다. 이것은 입력의 상대적인 값에 크게 반응하는 adaptation 기능을 이용하게 되며, 잡음 제거에 중요한 역할을 하게 된다. 스펙트럼 출력 단은 각 채널의 평균 firing rate를 이용하여 mean rate spectrum을 형성한다. 그리고 mean rate spectrum을 이용하여 특징 벡터를 추출하였다. 실험 결과는 청각 구조에 기초한 특징 추출이 다른 특징 추출 방법에 비해 잡음에서 더 향상된 성능을 가짐을 보였다.

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어류의 추출에 관한 연구 (Studies on the Extraction of Fish Oils)

  • 이근태;김상무;김장양
    • 한국수산과학회지
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    • 제18권1호
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    • pp.23-28
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    • 1985
  • The extraction rate of lipids from the chopped whole fish was studied with various solvents. Factors which can influence on the extraction rate such as temperature, moisture content, agitation speed and solvent were also examined. In the early stage of extraction, it is considered that the rapid extraction was attributed to cell destruction which occurs in chopping the whole fish and in the later stage, the extraction rate was increased linearly with extraction time. The effect of agitation on the extraction rate had a great influence on the early stage of extraction. In agitation speed of 100, 200, 300 and 500 r.p.m. the slopes of extraction curve were -0.075, -0.075, -0.069 and -0.064, respectively. Extractability between hydrophilic and hydrophobic solvent showed a great difference. It is suggested that extractability difference between acetone and isopropyl alcohol is due to acetone property which can not extract phospholipids in polar lipids. Extractability of lipids was increased with increasing temperature and decreased with increasing moisture content.

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Effect of Extraction Time on the Rheological Properties of Sericin Solutions and Gels

  • Yoo, Young Jin;Um, In Chul
    • International Journal of Industrial Entomology and Biomaterials
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    • 제27권1호
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    • pp.180-184
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    • 2013
  • Recently, silk sericin has attracted the attention of researchers owing to its useful properties as a biomaterial including 1) good wound healing and cell activities, 2) fast gelation character, and 3) high water retention property. In the present study, silk sericin was prepared using different extraction times in hot water and the effect of extraction time on the rheological properties of sericin solutions and gels was examined. It was found that the production yield of sericin increased with extraction time. The shear viscosity of sericin solutions and gels decreased with increasing extraction time due to a decrease in sericin molecular weight. When the sericin solution transformed to a gel, the viscosity increased and the shear thinning behavior was more evident. In addition, the shear stress measurements indicated that the slip between the sericin samples and the measuring plate of the rheometer was increased by the gelation of sericin. The compression strength of sericin gel could be increased remarkably (by more than 100 fold) by preparation using the freezing and thawing method.

초음파 추출물을 이용한 치콘의 면역활성 증진 (Enhancement of Immune-Potentiation of Cichorium endivia L. by Ultrasonification Extraction Process)

  • 권민철;한재건;;안주희;이달호;이현용
    • 한국약용작물학회지
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    • 제16권1호
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    • pp.9-15
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    • 2008
  • Immune-potentiation of Chicorium endivia L. were investigated on follows extracts associated with ultrasonification process at 60 kHz and showed the highest promotion of human B and T cell growth, about $10{\sim}20%$ compared to the control. The secretion of TNF-${\alpha}$ and IL-6 was also enhanced by the addition $(0.5mg/m{\ell})$ of the extracts. NK cell activation was Improved up to 1.37 times higher than the control, through adding extracts. It was also found that extracts from C. endivia L. could yield higher nitric oxide production from macrophage than Lipopolysaccaharides (LPS). It can be concluded that, in general, the extracts treated with ultrasonification has higher immune activity than others, possibly by higher yielding immune-modulatory activity than conventional extraction process. The optimum condition for the extraction of C. endivia L. is ethanol extraction at $60{\sim}100^{\circ}C$ associated with ultrasonification.

Morphological Feature Extraction of Microorganisms Using Image Processing

  • Kim Hak-Kyeong;Jeong Nam-Su;Kim Sang-Bong;Lee Myung-Suk
    • Fisheries and Aquatic Sciences
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    • 제4권1호
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    • pp.1-9
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    • 2001
  • This paper describes a procedure extracting feature vector of a target cell more precisely in the case of identifying specified cell. The classification of object type is based on feature vector such as area, complexity, centroid, rotation angle, effective diameter, perimeter, width and height of the object So, the feature vector plays very important role in classifying objects. Because the feature vectors is affected by noises and holes, it is necessary to remove noises contaminated in original image to get feature vector extraction exactly. In this paper, we propose the following method to do to get feature vector extraction exactly. First, by Otsu's optimal threshold selection method and morphological filters such as cleaning, filling and opening filters, we separate objects from background an get rid of isolated particles. After the labeling step by 4-adjacent neighborhood, the labeled image is filtered by the area filter. From this area-filtered image, feature vector such as area, complexity, centroid, rotation angle, effective diameter, the perimeter based on chain code and the width and height based on rotation matrix are extracted. To prove the effectiveness, the proposed method is applied for yeast Zygosaccharomyces rouxn. It is also shown that the experimental results from the proposed method is more efficient in measuring feature vectors than from only Otsu's optimal threshold detection method.

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폴리하드록시부틸레이트 회수를 위한 물리적 세포 파쇄용 돌기형 탄소나노튜브 분리막 제작 (Development of Physical Cell Lysis Using a Spiked CNT Membrane for Polyhydroxybutyrate Recovery)

  • 문지원;백영빈
    • 멤브레인
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    • 제33권6호
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    • pp.390-397
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    • 2023
  • 석유기반 플라스틱의 대체제인 폴리하드록시부틸레이트(polyhydroxybutyrate, PHB)의 기존 추출방법은 분자량 감소 및 물성 변형을 일으킨다. 본 연구에서는 기능화 된 탄소나노튜브(carbon nanotube, CNT)를 부착한 돌기형 탄소나노튜브 분리막의 여과를 통해 물리적 파쇄를 발생시켜 미생물 내 축적된 PHB를 추출하고자 하였다. 돌기형 탄소나노튜브 분리막의 물리적 파쇄를 확인하기 위해 대장균 용액으로 여과 실험을 수행하여 불활성화를 관찰하였다. 또한 PHB를 축적한 미생물 용액의 여과를 수행하여 PHB가 추출되었는지 확인하였더니 가장 대표적인 추출방법인 chloroform과 비교하여도 여과로 인한 추출이 4% 높은 성능을 가진 것을 관찰하였다. 본 결과를 통해 친환경적 바이오 플라스틱 회수를 위한 돌기형 탄소나노튜브 분리막의 적용 가능성을 확인하였다.

파삼의 항암활성 증진이 가능한 고압 추출 공정 (High Pressure Extraction Process of Low Quality Fresh Ginseng for Enhancing Anticancer Activities)

  • 하지혜;김영;김승섭;정명훈;정헌상;정재헌;유광원;이현용
    • 한국약용작물학회지
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    • 제17권6호
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    • pp.397-406
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    • 2009
  • The low quality fresh ginseng was extracted by water at $80^{\circ}C$ and 240 bar for 20 min (HPE, High pressure extraction process). The cytotoxicity on human normal kidney cell (HEK293) and human normal lung cell (HEL299) of the extracts from HPE showed 28.43% and 21.78% lower than that from conventional water extraction at $100^{\circ}C$ in adding the maximum concentration of $1.0\;mg/m{\ell}$. The human breast carcinoma cell and lung adenocarcinoma cell growth were inhibited up to about 86%, in adding $1.0\;mg/m{\ell}$ of extracts from HPE. This values were 9-12% higher than those from conventional water extraction. On in vivo experiment using ICR mice, the variation of body weight of mice group treated fresh ginseng extracts from HPE of 100 mg/kg/day concentration was very lower than control and other group. The extracts from HPE was showed longer survival times as 35.65% than that of the control group, and showed the highest tumor inhibition activities compared with other group, which were 70.64% on Sarcoma-180 solid tumor cells. On the high performance liquid chromatogram (HPLC), amount of ginsenoside-$Rg_2$, $Rg_3$, $Rh_1$ and $Rh_2$ on fresh ginseng were increased up to 43-183% by HPE, compared with conventional water extracts. These data indicate that HPE definitely plays an important role in effectively extracting ginsenoside, which could result in improving anticancer activities. It can be concluded that low quality fresh ginseng associated with this process has more biologically compound and better anticancer activities than that from normal extraction process.

청각신경 시냅스의 적응 효과를 이용한 인공와우 어음처리 알고리즘의 개선에 대한 시뮬레이션 연구 (A Simulation Study on Improvements of Speech Processing Strategy of Cochlear Implants Using Adaptation Effect of Inner Hair Cell and Auditory Nerve Synapse)

  • 김진호;김경환
    • 대한의용생체공학회:의공학회지
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    • 제28권2호
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    • pp.205-211
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    • 2007
  • A novel envelope extraction algorithm for speech processor of cochlear implants, called adaptation algorithm, was developed which is based on a adaptation effect of the inner hair cell(IHC)/auditory nerve(AN) synapse. We achieved acoustic simulation and hearing experiments with 12 normal hearing persons to compare this adaptation algorithm with existent standard envelope extraction method. The results shows that speech processing strategy using adaptation algorithm showed significant improvements in speech recognition rate under most channel/noise condition, compared to conventional strategy We verified that the proposed adaptation algorithm may yield better speech perception under considerable amount of noise, compared to the conventional speech processing strategy.

Biological Image Edge Extraction Based on Adaptive Beamlet Transform

  • Nguyen, Van Hau;Woo, Kyung-Haeng;Choi, Won-Ho
    • 융합신호처리학회논문지
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    • 제12권2호
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    • pp.83-90
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    • 2011
  • In cell biology area, microscopy enables detecting objects inside cells that are stained or fluorescently tagged. It is disadvantageous for observing these objects because of the noisy characteristics of their environmental surrounding. In this paper, a framework is proposed to increase the throughput and reliability for analysis of these images. First, we apply adaptive beamlet transform to extract edges meaningfully followed by orientation, location, and length in different scales. Then, a post-process is implemented to extend and map them onto original image. Our proposed scheme is compared with Canny edge detector and conventional beamlet transform from four evaluation aspects. It produces better results when experiments are conducted on real images. Much better results for observing internal parts make this framework competitive for analysis of cell images.

JTAG을 이용한 휴대폰 포렌식 데이터 수집 (Forensic Data Acquisition on Cell Phone using JTAG Interface)

  • 김건우;류재철
    • 대한전자공학회:학술대회논문집
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    • 대한전자공학회 2008년도 하계종합학술대회
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    • pp.333-334
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    • 2008
  • With the role of cell phones in today's society as a digital personal assistant as well as the primary tool for personal communication, it is possible to imagine the involvement of cell phones in almost any type of crime. The progression of a criminal investigation can hinge on vital clues obtained from a cell phone. This paper will be concentrated on CDMA system phones and focus on the data extraction for cell phone forensics. Especially, the data acquisition method of JTAG interface access to memory chip will be covered.

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