• 제목/요약/키워드: Cell Count

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PAC 및 PAS 첨가에 따른 상업용 액상 종균제의 총균수 변화에 관한 연구 (A study on the total cell count variation of commercial liquid seed by adding PAC and PAS)

  • 박미자;박경식;김승재
    • 환경위생공학
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    • 제16권2호
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    • pp.79-90
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    • 2001
  • Commercial liquid seeds are used for supplying active microbial flora to organic wastewater treatment plants of high feed-to-microorganism ratio and to maintain optimal microbial condition during unsteady state operation of the biological wastewater treatment plant. In addition to bacterial cells, the liquid weeds contain various additives for special purposes as well as organic substrates for energy supply. The additives give physical stability for the maintenance of microbial decomposition activity and ability to control the overgrowth of seed strains. In this work, the effects of addition of two kinds of typical substrate additives, poly aluminum chloride(PAC) and poly aluminum sulfate(PAS) on the consitutional total cell counts(CFU/ml) of four kinds of reorganization liquid seeds(RLS I, RLS II, RLS III and RLS IV) were studied experimentally. The addition of PAC and PAS gave negative effect on TCC constitution for the four seeds studied.

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Optimization of Food Waste Fermentation for Probiotic Feed Production with Yeast Kluyveromyces marxianus

  • Lee, Ki-Young;Yu, Sung-Jin;Yu, Seung-Yeng
    • 한국자원리싸이클링학회:학술대회논문집
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    • 한국자원리싸이클링학회 2001년도 정기총회 특별강연 및 춘계학술연구발표회(2)
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    • pp.121-125
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    • 2001
  • For the probiotic feed production, aerobic liquid fermentation of pulverized food wastes was attempted with a yeast Kluyveromyces marxianus. After grinding finely, optimal fermentation conditions of the substrate was investigated by shaking culture. The most active growth of the yeast was shown at solid content of 10%. The proper addition of urea(0.5g/l), o-phosphate(0.4g/l), molasses(4g/l), and yeast extract (1g/1) increased cell growth rate and viable cell count. For optimizing, the nutrients were all added to substrate and fermentation was carried in 2 litre jar fermenter. For the stimulation of hydrolyzing enzyme excretion, mixed culture with Aspersillus oryzae was also conducted. In 12 hours of fermentation, viable cell count of the yeast Kluyveromyces marxianus amounted to the number of 1.4 $\times$10$^{10}$ /1 in the culture medium.

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부산지역 음용수의 세균학적 특성 (Bacteriological Characteristics of Drinking Water in Pusan Area)

  • 김용관
    • 한국환경보건학회지
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    • 제19권2호
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    • pp.34-39
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    • 1993
  • One hundred and eighty-seven water samples were collected from 23 of spring water, 2 of ground water, 1 of tap water in Pusan area and 3 of natural mineral waters. Total coliform group, fecal coliform, viable cell count and microflora were investigated to evaluate water quality of drinking water. The results were as follows: range and geometric mean value of total coliform and fecal coliform MPN's of spring water were 0~1,500/100 ml, 85/100 ml and 0~460/100 ml, 24/100 ml but coliform group was not detected in the samples of tap water and natural mineral water. Viable cell count of spring water, ground water and tap water were lower as 100 cell than the criteria for drinking water but that of natural mineral water was higher as 6.5X 10$^2$~7.4X 10$^3$ /ml. Predominant speces among the 219 strains isolated from the samples were 19.6% Aeromonas spp., 19.2% Enterobacteriaceae, 16% Acinetobacter spp. Especially, spring water and vessels were contaminated by Hafnia spp. and Providencia Spp, inhabitant of the oral cavity.

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Acute Variation of Hematological Parameters during 622 km Ultra-Marathon

  • Shin, Kyung-A;Kim, Young-Joo
    • 대한의생명과학회지
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    • 제23권3호
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    • pp.208-214
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    • 2017
  • To investigate the effects of strenuous physical exercise on commonly used hematological markers in subjects the intensive long running. Blood samples were obtained from nineteen participants in a 622 km ultra-marathon race before, 300 km and immediately after completion of the 622 km ultra-marathon. Samples were analyzed for total white cell count (WBC) and differential, total red cell count (RBC), hemoglobin, hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), red cell distribution width (RDW), platelets, mean platelets volume (MPV), platelets distribution width (PDW). Significant increases were found in WBC, neutrophil and platelets at 622 km compared to the pre-race. RBC, hemoglobin and hematocrit decreased statistically significantly the race at 300 km and 622 km compared to pre-race. A wide range of hematological perturbations occur during 622 km ultra-marathon running but it was physiological changes within a reference range. The 622 km ultra-marathon is less likely to cause clinically significant hematologic changes in athletes.

Design and Optimization of Full Comparator Based on Quantum-Dot Cellular Automata

  • Hayati, Mohsen;Rezaei, Abbas
    • ETRI Journal
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    • 제34권2호
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    • pp.284-287
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    • 2012
  • Quantum-dot cellular automata (QCA) is one of the few alternative computing platforms that has the potential to be a promising technology because of higher speed, smaller size, and lower power consumption in comparison with CMOS technology. This letter proposes an optimized full comparator for implementation in QCA. The proposed design is compared with previous works in terms of complexity, area, and delay. In comparison with the best previous full comparator, our design has 64% and 85% improvement in cell count and area, respectively. Also, it is implemented with only one clock cycle. The obtained results show that our full comparator is more efficient in terms of cell count, complexity, area, and delay compared to the previous designs. Therefore, this structure can be simply used in designing QCA-based circuits.

Nodal tumor response according to the count of peripheral blood lymphocyte subpopulations during preoperative chemoradiotherapy in locally advanced rectal cancer

  • Heo, Jaesung;Oh, Young-Taek;Noh, O Kyu;Chun, Mison;Park, Jun-Eun;Cho, Sung-Ran
    • Radiation Oncology Journal
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    • 제34권4호
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    • pp.305-312
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    • 2016
  • Purpose: The objective of this prospective study was to evaluate the relationship between the circulating lymphocyte subpopulation counts during preoperative chemoradiotherapy (CRT) and tumor response in locally advanced rectal cancer. Materials and Methods: From August 2015 to June 2016, 10 patients treated with preoperative CRT followed by surgery were enrolled. Patients received conventional fractionated radiotherapy (50.4 Gy) with fluorouracil-based chemotherapy. Surgical resection was performed at 4 to 8 weeks after the completion of preoperative CRT. The absolute blood lymphocyte subpopulation was obtained prior to and after 4 weeks of CRT. We analyzed the association between a tumor response and change in the lymphocyte subpopulation during CRT. Results: Among 10 patients, 2 (20%) had evidence of pathologic complete response. In 8 patients with clinically node positive, 4 (50%) had nodal tumor response. All lymphocyte subpopulation counts at 4 weeks after CRT were significantly lower than those observed during pretreatment (p < 0.01). A high decrease in natural killer (NK) cell, count during CRT (baseline cell count - cell count at 4 weeks) was associated with node down staging (p = 0.034). Conclusion: Our results suggest that the change of lymphocyte subset to preoperative CRT may be a predictive factor for tumor response in rectal cancer.

고동아말감과 Glass ionomer-silver cement의 생물학적 평가에 관한 연구 (A BIOLOGICAL EVALUATION OF HIGH COPPER AMALGAM AND GLASS IONOMER-SILVER CEMENT)

  • 오병원;최호영;민병순;박상진
    • Restorative Dentistry and Endodontics
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    • 제15권2호
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    • pp.1-16
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    • 1990
  • This study was to evaluate the cytotoxic effect in vitro and the tissue response within the rat peritoneal cavity to high copper amalgam and glass ionomer-silver cement, suggested for use as a retrograde endodontic filling material. In the cytotoxicity experiment, the radioactively ($^{51}Cr$) labeled L929 mouse fibroblasts were employed to determine the relative cytotoxicity of two experimental materials. Those materials were evaluated immediately after set and after one and seven days setting. In the tissue response experiment, two experimental materials were to evaluate mean peritoneal cellular count, differential cell count and the content of silver and copper in pooled packed cells and eluate samples taken by peritoneal lavage technique, and compared with surgical control after one day. two, four and six weeks of implantation. The results were as following: 1. High copper amalgam exhibited significant cytotoxicity immediately after set but showed no sign of toxicity after one day and seven days setting materials. 2. Glass ionomer-silver cement showed no sign of toxicity immediately after set and after one day and seven days setting. 3. High copper amalgam and glass ionomer-silver cement groups produce no significant difference in the mean peritoneal cell count when compared with the surgical control group after one day, two and four weeks of implantation. Surgical control group exhibited significantly a greater cell count when compared with the High copper amalgam group after six weeks. 4. High copper amalgam group increased significantly in the percentage macrophages after four and six weeks of implantation when compared with surgical control group. 5. The trace metal analysis involved an increased silver content in the elutes and an increased copper content in the packed cells of high copper amalgam group, and an increased silver content in the packed cells and elutes of glass ionomer-silver cement group.

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中脘(중완)(CV12) 및 천추(天樞)(ST25) 산양산삼약침 처치가 흰쥐의 혈액성상 및 싸이토카인에 미치는 영향 (Effects of cultivated wild ginseng pharmacopuncture at CV12 and ST25 on blood cell composition and cytokine levels in rats)

  • 이종욱;이향숙;심성윤;이준무
    • Korean Journal of Acupuncture
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    • 제23권3호
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    • pp.99-109
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    • 2006
  • Objectives : To investigate whether cultivated wild ginseng pharmacopuncture at CV12 and/or ST25 has any immune-enhancing effect in normal rats. Methods : Effects of cultivated wild ginseng pharmacopuncture at CV12 and/or ST25 on blood cell counts, cell composition, cytokine and plasma protein levels were investigated in normal rats. Results : Red blood cell count, hematocrit and hemoglobin levels significantly increased in cultivated wild ginseng pharmacopuncture groups compared with those of the normal group. There was, however, no significant difference in white blood cell (WBC) count and WBC differential count between the normal group and the pharmacopuncture groups. Cultivated wild ginseng pharmacopuncture groups had significantly higher levels of tumor necrosis $factor-{\alpha}\;(TNF-{\alpha})$ and interleukin-6 (IL-6) than the normal group while no significant difference between groups was found in interleukin-$1{\beta}\;(IL-1{\beta})$ level. Total protein and albumin levels were not different between groups. Conclusions : The results suggest that cultivated wild ginseng pharmacopuncture at CV12 and/or ST25 may have no immune-enhancing effect in normal rats.

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시유의 유통기간 결정에 관한 학문적 고찰 (Scientific Consideration in Determining Shelf Life of Market Milk)

  • 최석호
    • Journal of Dairy Science and Biotechnology
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    • 제22권1호
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    • pp.27-35
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    • 2004
  • The shelf lift of market milk should be determined based on the flavor which is influenced by environmental and sanitary conditions of dairy farm, milk processing plant, and storage and transportation facility as well as compositional quality, such as protein and fat, of the milk itself. The legal shelf life of market milk is often limited by microbiological quality, e.g. total bacterial count, coliform count, and food poisoning bacteria. The bacteria involved with milk spoilage and poisoning are originated from bacteria contaminating milk after pasteurization or spores surviving the heat treatment of pasteurization. The important factors which influence the shelf life of market milk are microbiological quality of raw milk, pasteurization condition, post-pasteurization contamination, and temperature during storage and transportation. The organoleptic quality and shelf life of market milk should be further improved by satisfying the consumer's taste, which depends on somatic cell count and bacterial count of milk, feed quality, foreign substance in milk, and physical treatment during processing and transportation.

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An Analysis of Panel Count Data from Multiple random processes

  • 박유성;김희영
    • 한국통계학회:학술대회논문집
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    • 한국통계학회 2002년도 추계 학술발표회 논문집
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    • pp.265-272
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    • 2002
  • An Integer-valued autoregressive integrated (INARI) model is introduced to eliminate stochastic trend and seasonality from time series of count data. This INARI extends the previous integer-valued ARMA model. We show that it is stationary and ergodic to establish asymptotic normality for conditional least squares estimator. Optimal estimating equations are used to reflect categorical and serial correlations arising from panel count data and variations arising from three random processes for obtaining observation into estimation. Under regularity conditions for martingale sequence, we show asymptotic normality for estimators from the estimating equations. Using cancer mortality data provided by the U.S. National Center for Health Statistics (NCHS), we apply our results to estimate the probability of cells classified by 4 causes of death and 6 age groups and to forecast death count of each cell. We also investigate impact of three random processes on estimation.

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