• The Korean Journal of Pesticide Science
• /
• v.6 no.1
• /
• pp.9-15
• /
• 2002

The insecticide constituents of several Celastraceae family plants including Celastrus angulatus, Celastrus orbiculatus, Celastrus flagellaris, Euonymus verrucosides, Euonymus forunei and Euonymus phellomana were studied by bioassay-guided fractionation. All structures of sesquiterpene polyol esters and alkaloids have been elucidated by UV, IR, MS, NMR and x-ray diffraction evidences. These compounds exhibited insecticidal, antifeedant and narcotic activities against Mythimna separata.

• Natural Product Sciences
• /
• v.17 no.2
• /
• pp.135-141
• /
• 2011

Celastrus orbiculatus has been widely used as a traditional medicine for the treatment of many diseases including rheumatoid arthritis and odontalgia. In the present study, anti-metastatic activity of a methanolic extract from C. orbiculatus (MCO) was studied. A gelatin zymographic assay revealed that MCO has potent inhibitory effects on MMP-2 and MMP-9 activities in B16F10 melanoma cells. Moreover, MCO attenuated MMP expression via down-regulation of NF-${\kappa}$B translocation to the nucleus. Melanoma cell migration and invasion were also down-regulated by MCO. In addition, MCO significantly suppressed lung metastasis in an in vivo model. These results strongly suggest that MCO may possibly be used as a valuable anti-metastatic agent for cancer treatment.

• Journal of Nutrition and Health
• /
• v.40 no.6
• /
• pp.493-499
• /
• 2007

Celastrus orbiculatus (CO) has been used as a traditional herb medicine to treat fever, chill, joint pain, edema, rheumatoid arthritis and bacterial infection in China and Korea. In this study, we investigated anticarcinogenic effects of Celastrus orbiculatus (CO). CO was extracted with methanol (COM), and then further fractionated into four different types: methanol (COMM), hexane (COMH), butanol (COMB) and aqueous (COMA) partition layers. We determined the cytotoxicity of these four partitions in four kind of cancer cell lines, such as HepG2, MCF-7, HT29 and B16F10 Cells by MTT assay. Among various partition layers of CO, the COMM showed the strongest cytotoxic effects on cancer cell lines we used. We also observed quinone reductase (QR) induced effects in all partition layers of CO on HepG2 cells. The QR induced effects of COMM on HepG2 cells at 80 ${\mu}$ g/mL concentration indicated 3.28 to a control value of 1.0. The COMM showed the highest induction activity of quinone reductase on HepG2 cells among the other partition layers. Although further studies are needed, the present work suggests that CO may be a chemopreventive agent for the treatment of human cells.

• Agrochemical news magazine
• /
• v.15 no.5 s.122
• /
• pp.84-85
• /
• 1994

• The Journal of Korean Medicine
• /
• v.29 no.4
• /
• pp.94-103
• /
• 2008

Objectives: The aim of this study was to determine whether celastrol, the triterpenoid component of Celastrus orbiculatus, offers neuroprotection against Parkinson's disease (PD) in mice administered 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine(MPTP). Methods: We examined how celastrol affected MPTP-induced neuronal loss of tyrosine hydroxylase (TH)-positive dopaminergic neurons in substantia nigra pars compacta (SNpc) in the midbrain of mice. C57BL/6J mice were divided into four groups: (1) saline-saline, (2) saline-celastrol, (3) MPTP-saline, and (4) MPTP-celastrol. The mice were injected intraperitoneally (i.p.) with four administrations of MPTP (18mg/kg) at 2 h intervals and then i.p. administered celastrol (3mg/kg) two times at 12 h after last celastrol administration. Expression of TH on the SNpc of brain tissues were analyzed at 7 days after the treatments by immunohistochemistry and Western blot. Results: Immunohistochemical analysis using TH antibody showed that celastrol provided significantly protective effects against MPTP-induced loss of TH-positive dopaminergic neurons in the SNpc region of the midbrain of mice. Our Western blot study also showed that celastrol significantly inhibits the MPTP-induced neuronal damage via the up-regulation of TH protein levels in MPTP mice. Conclusions: The present results suggest that it may be possible to use celastrol for the prevention of nigral degenerative disorders including PD, caused by exposure to toxic substances.

• Korean Journal of Pharmacognosy
• /
• v.28 no.4
• /
• pp.174-178
• /
• 1997

Methanol extracts from 450 plants were screened for muttidrug-resistance reversing activity using drug sensitive KB-3-1 and multidrug-resistant KB-Vl cells. Among them, the extracts of Cynanchum wilfordii, Torilis japonica, Celastrus orbiculatus, Melia toosendan and Teminialia chebula strongly potentiated vinblastine cytotoxicity in KB-Vl cells. But their cytotoxicities to both sensitive KB-3-1 and resistant KB-Vl cells were in the same order of magnitude. These results indicate that the above samples would contain the active principles which do not exert their ativity solely by cytotoxicity.

• Journal of Life Science
• /
• v.19 no.1
• /
• pp.52-57
• /
• 2009

This study was carried out to investigate the antioxidative and antimicrobial activities of Celastrus orbiculatus (CO). CO was extracted with methanol and then further fractionated with n-hexane (COMH), butanol (COMB), methanol (COMM) or aqueous (COMA) to get active fractions. The antioxidative activity of fractions from CO was investigated by measuring the scavenging activities of CO against DPPH radical, peroxynitrite ($ONOO^-$) and reactive oxygen speicies (ROS). Among the various solvent fractions, the COMB showed a marked scavenging effect against DPPH radical, peroxynitrite ($ONOO^-$) and reactive oxygen species(ROS). The antimicrobial activity was increased in proportion to its concentration by the paper disk method. Among the various solvent fractions, the COMM and COMB fractions of CO showed strong antimicrobial activities. The results suggest that The CO may be suitable for development as a food preservative and alternative antioxidant.

• Korean Journal of Medicinal Crop Science
• /
• v.15 no.4
• /
• pp.271-275
• /
• 2007

Naturally occurring substances are important biomedical resources with low toxicity and ethnopharmacology-based efficacy. Four out of 45 extracts (Celastrus orbiculatus, Cercis chinensis, Stephanadra incisa, and Weigela subsessilis) prepared from the bark of Korea Forest plants exhibited more than 50% of inhibition on $TNF-{\alpha}$ production in lipopolysaccharide (LPS)-activated RAW264.7 cells at $100\;{\mu}g/m{\ell}$. In particular, potential inhibitory components of 4 extracts showed more than 50% inhibition seemed to be concentrated in methylene chloride (MC) fraction from C. orbiculatus, in ethyl acetate (EtOAc) fraction from C. chinensis and in hexane (Hx) fraction from S. incisa, whereas inhibitory activities of W. subssilis were broadly seen in non-polar solvent fractions such as Hx, MC and EtOAc. Therefore, our results suggest that extracts from C. orbiculatus, C. chinensis, S. incisa and W. subsessilis may be developed as a therapeutic remedy against $TNF-{\alpha}-mediated$ diseases such as rheumatoid arthritis or further fractionated to isolate active components having $antiTNF-{\alpha}$ inhibitory activity.

• Journal of Haehwa Medicine
• /
• v.4 no.1
• /
• pp.445-448
• /
• 1995

Although significant progress has been made during the past four decades in the treatment of human neoplastic disease, effective chemotherapeutic treatments are nevertheless far from satisfactory to patients suffering from cancer, particularly to those with the stomach cancer which is most prevalent in Korea. To find out the effective anticancer drugs, especially human stomach cancer, we tested cytotoxic activities of several medicinal herbs against human stomach cancer cell line, SNU-1. Evodia officinalis, Melia toosendan, Sinomenium acutum, Galla Haeepensis, Celastrus orbiculatus and Taxus caespitosa showed a significant cytotoxic activities.

• Applied Biological Chemistry
• /
• v.43 no.3
• /
• pp.174-178
• /
• 2000

To search CDK4/Cyclin D1 enzyme inhibition materials, methanol extracts of native eighty seven plant species in thirty seven families were screened in vitro for their inhibiting activities against CDK4/Cyclin D1 enzyme which are control to the normal cell division cycle in human body. Extracts of Paeonia suffruticosa, Saurus chinensis, Sanguisorba officinalis and Celastrus orbiculatus among them significantly inhibited above fifty percent $(in\;5\;{\mu}g/ml)$ against CDK4/ Cyclin D1 enzyme. Especially, the extracts of P. suffruticosa and S. officinalis showed moderately strong inhibition. Also, cryptotanshinone was identified as active compound from a extracts of Salvia mitiorrhiza by spectroscopic analyses including 2D NMR experiments.