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Anti-metastatic Effects of Celastrus orbiculatus Extract in B16F10 Melanoma Cells  

Jeon, Hoon (College of Pharmacy, Woosuk University)
Publication Information
Natural Product Sciences / v.17, no.2, 2011 , pp. 135-141 More about this Journal
Abstract
Celastrus orbiculatus has been widely used as a traditional medicine for the treatment of many diseases including rheumatoid arthritis and odontalgia. In the present study, anti-metastatic activity of a methanolic extract from C. orbiculatus (MCO) was studied. A gelatin zymographic assay revealed that MCO has potent inhibitory effects on MMP-2 and MMP-9 activities in B16F10 melanoma cells. Moreover, MCO attenuated MMP expression via down-regulation of NF-${\kappa}$B translocation to the nucleus. Melanoma cell migration and invasion were also down-regulated by MCO. In addition, MCO significantly suppressed lung metastasis in an in vivo model. These results strongly suggest that MCO may possibly be used as a valuable anti-metastatic agent for cancer treatment.
Keywords
Celastrus orbiculatus; metastasis; invasion; MMP;
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