• 대한의생명과학회지
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• 제24권4호
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• pp.426-429
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• 2018

Chronic eosinophilic leukemia (CEL) is characterized by eosinophilia and organ damage. Imatinib is widely used for treating CEL, chronic myeloid leukemia (CML) and acute myeloid leukemia (AML). Unfortunately, the cancer cells gain resistance against the drug after prolonged molecular-targeted therapies. Imatinib-resistant EoL-1 (EoL-1-IR) cells were produced from chronic eosinophilic leukemia cells (EoL-1) after treatment with imatinib for a long duration. Two-dimensional electrophoresis (2-DE) analysis revealed numerous protein variations in the EoL-1 and EoL-1-IR sub-types. Compared to the EoL-1 cells, expression levels of TIP49, RBBP7, ${\alpha}$-enolase, adenosine deaminase, C protein, galactokinase, eukaryotic translation initiation factor, $IFN-{\gamma}$, and human protein homologous to DROER were increased, whereas core I protein, proteasome subunit p42, heterogeneous ribonuclear particle protein, chain B, and nucleoside diphosphate were decreased in the EoL-1-IR cells. Taken together, these results contribute to understanding the pathogenic mechanism of drug-resistant diseases.

• 원예과학기술지
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• 제19권4호
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• pp.505-510
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• 2001

춘파 당근 종자의 발아능 향상을 위한 적정 SMP조건 구명과 처리기간 중 종자의 생리적 변화를 관찰하기 위하여 이나리, 만산5촌, 무쌍5촌, 홍심5촌 등 4개 품종을 공시하여 실험을 실시하였다. SMP 처리에서 물질(Micro-Cel E)에 대한 물의 첨가배율이 높아지고 처리기간이 길어질수록 발아율이 높고, $T_{50}$이 단축되었지만 처리과정 중 일부 처리에서는 유근이 돌출되었다. 품종에 따른 SMP조건이 다소 달랐다. SMP의 적정 종자 : 물질 : 수분의 비율은 5 : 3: 10.5이고 적정 처리기간은 만산5촌이 3일, 그 외 3품종은 5일이었다. SMP적정처리 온도는 $20-25^{\circ}C$인 것으로 나타났다. SMP 기간 중 종자의 변화 중에서 수분흡수율은 처리 후 1시간까지 급속하게 증가하고 그 후 6기간까지 약 38%로 증가하다가, 24시간째에는 품종에 따라 43%-49%를 보였다. 그 이후부터는 처리종료시간까지 일정하게 유지되었다. 종자의 전기전도도는 6시간까지 감소하여 $100{\mu}S{\cdot}cm^{-1}$ 내외에서 거의 변화를 보이지 않았고, pH는 초기에 약간 높아지다가 처리기간이 경과할수록 원래의 pH로 되돌아오는 경향을 보였다.

• 생물환경조절학회지
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• 제13권3호
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• pp.135-142
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• 2004

본 연구는 당근과 양파에서 발아력 증진에 미치는 osmotic priming과 SMP효과를 비교하기 위해 수행되었다. Osmotic priming과 SMP 처리과정중 작물별 수분흡수율은 처리 1시간 후 급속한 수분흡수가 이루어졌고, 두 작물 모두 osmotic priming이 SMP보다 수분흡수 속도가 빨랐다. 이러한 경향은 처리 4시간까지 유지되었다. 처리최종일의 수분흡수율은 당근과 양파에서 osmotic priming이 SMP보다 2%높았다. Osmotic priming과 SMP 처리는 두 작물 모두 발아율을 향상시키지 못했지만 $T_{50}$ 및 MDG는 단축되어 조기발아 하였으며, 이러한 경향은 저온인 $15^{\circ}C$에서 더욱 현저하였다. Osmotic priming과 SMP 처리 후 초기함수율로 재건조하면 당근에서는 발아속도에 큰 차이가 없었으나, 양파에서는 표면건조에 비해 발아속도가 지연되었다. Osmotic priming과 SMP 상호처리간 발아력을 비교한 결과 두 작물 모두 SMP 처리가 osmotic priming보다 발아촉진에 효과적이었다. 당근과 양파종자를 osmotic priming과 SMP 처리하면 묘출현율도 향상되었고, 묘출현에 소요되는 일수를 단축시켜 신속한 묘출현을 유도하였다. 그러나 묘출현에 이은 유묘의 초기생육에는 통계적인 유의성은 인정되지는 않았으나 osmotic priming과 SMP 처리된 종자는 무처리에 비해 향상되었다.

• Journal of Preventive Medicine and Public Health
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• 제55권3호
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• pp.280-288
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• 2022

Objectives: One of the most widely used pesticides today is chlorpyrifos (CPF). Cytochrome P450 (CYP)2B6, the most prominent catalyst in CPF bioactivation, is highly polymorphic. The objective of our study was to evaluate the role of CYP2B6^*6, which contains both 516G>T and 785A>G polymorphisms, in CPF toxicity, as represented by the concentration of 3,5,6-trichloro-2-pyridinol (TCPy), among vegetable farmers in Central Java, Indonesia, where CPF has been commonly used. Methods: A cross-sectional study was conducted among 132 vegetable farmers. Individual socio-demographic and occupational characteristics, as determinants of TCPy levels, were obtained using a structured interviewer-administered questionnaire and subsequently used to estimate the cumulative exposure level (CEL). TCPy levels were detected with liquid chromatography-mass spectrometry. CYP2B6^*6 gene polymorphisms were analyzed using a TaqMan^® SNP Genotyping Assay and Sanger sequencing. Linear regression analysis was performed to analyze the association between TCPy, as a biomarker of CPF exposure, and its determinants. Results: The prevalence of CYP2B6^*6 polymorphisms was 31% for ^*1/^*1, 51% for ^*1/^*6, and 18% for ^*6/^*6. TCPy concentrations were higher among participants with CYP2B6^*1/^*1 than among those with ^*1/^*6 or ^*6/^*6 genotypes. CYP2B6^*6 gene polymorphisms, smoking, CEL, body mass index, and spraying time were retained in the final linear regression model as determinants of TCPy. Conclusions: The results suggest that CYP2B6^*6 gene polymorphisms may play an important role in influencing susceptibility to CPF exposure. CYP2B6^*6 gene polymorphisms together with CEL, smoking habits, body mass index, and spraying time were the determinants of urinary TCPy concentrations, as a biomarker of CPF toxicity.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 1995년도 춘계학술대회 논문집
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• pp.154-159
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• 1995

To achieve a high aspect ration of metal contact, buried contact solar cell scribe the silicon surface using laser. The Q-switched NdLYAG laser which has 1.064$\mu\textrm{m}$ wavelength use for silicon scribing with 25~40$\mu\textrm{m}$ width and 20~200$\mu\textrm{m}$ depth capabilities. The 2~3% shading losses are very low campared to the screen printing solar cell. In this paper, we investigate the silicon scribing theory and pratice, scribing system for BCSC processing.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 1986년도 추계학술대회
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• pp.525.1-525
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• 1986

To clone ${\beta}$-glucosidase gene from Cellulomonas sp. a gene library was constructed using E. coli JM83 pUC9. Among 2,500 pseudotransformants obtained, 20 clones developed yellow color on the p-nitrophenyl- -D-glucopyranoside filter paper These 20 clones were classified into three groups based on the results of activity staining using nondenaturating polyacrylamide gel electrophoresis and restriction enzyme digestions. Among the three groups, only one group containing pCEl plasmid has specificity for cellobiose.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.356.3-357
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• 2002

Metoprolol (MT) is one kinds of adrenergic beta-blockers. Its (S)-enantiomer is known to be more active than the (R). Recently. the x-ray structure of beta-blocker. (S)-propranolol (a-naphthyl analogue), complexed in a mould fungal cellulase. Cel7A. was reported and the (R)-form did not build any complex. And in our previous study the conformation and stability of MT in carboxymethylated beta-cyclodextrin (BCD) complex was determined by NMR. HPLC, UV and electrophoresis measurement. (omitted)

• 전력전자학회:학술대회논문집
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• 전력전자학회 1999년도 전력전자학술대회 논문집
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• pp.419-422
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• 1999

This paper deals with stand-alone Photovoltaic system(SPVS) with charger/discharge controller. Main power source of SPVS are generally solar cel and battery. Therefore SPVS can be classified into variable types in accordance with connection type between battery and solar cell. SPVS with charger/discharge controller which can operate solar cell a maximum power point is suggested and designed with instantaneous controller. And system operating characteristics are verified by experiment with a laboratory prototype in this paper.

• 한국자원식물학회지
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• 제3권1호
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• pp.1-30
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• 1990

The traditional plant imprownent methods consisted of pure line selection, cross breeding, heterosis breeding, polyploid breeding, mutati-onbreeding, ect.Biotechmoiogy is divided into gene spliclng , monocle-nal antibodies , protein engineering , agricultural research, and microbiological engineering. Of these , high plants deal with agricultural research, and the importent part of which is tissue culture and celLculture , Tissue .culture and cell culture are again divided into embryoculture, test tube fertilization, anther and pollen culture, somatichybridization , transformation, recombination, recombinant DNA moleculehybrid plasmid, ect For these haploid production, protoplast culture,protoplast fusion, selection and propagation, ect. , the technical sett-lement is needed.

• 한국정보과학회:학술대회논문집
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• 한국정보과학회 2001년도 가을 학술발표논문집 Vol.28 No.2 (1)
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• pp.70-72
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• 2001

기존의 CBF 기법은 데이타의 차원이 증가함에 따라 검색 성능이 급격히 저하되는 ‘Dimensional Curse’문제를 해결하기 위해 제안되었다. 그러나, 데이타의 양이 증가하고 차원이 증가할수록 검색 성능이 선형적인 감소를 보인다. 따라서, 본 논문에서는 CBF 기법의 성능 향상을 위해 멀티 디스크 환경을 기반으로 하는 병렬 CBF 기법을 제안한다. 제안하는 병렬 CBF 기법은 멀티 디스크 환경하에서 CBF가 지니는 특성을 이용하여 시그니쳐와 특징 벡터 데이타의 수평 분할 방법을 사용한다. 이를 통해, 제안하는 기법은 디스크 개수에 비례하여 선형적인 검색성능 향상을 가져온다.