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Gene Expression Profiling in the Pituitary Gland of Laying Period and Ceased Period Huoyan Geese

  • Luan, Xinhong;Cao, Zhongzan;Xu, Wen;Gao, Ming;Wang, Laiyou;Zhang, Shuwei
    • Asian-Australasian Journal of Animal Sciences
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    • 제26권7호
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    • pp.921-929
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    • 2013
  • Huoyan goose is a Chinese local breed famous for its higher laying performance, but the problems of variety degeneration have emerged recently, especially a decrease in the number of eggs laid. In order to better understand the molecular mechanism that underlies egg laying in Huoyan geese, gene profiles in the pituitary gland of Huoyan geese taken during the laying period and ceased period were investigated using the suppression subtractive hybridization (SSH) method. Total RNA was extracted from pituitary glands of ceased period and laying period geese. The cDNA in the pituitary glands of ceased geese was subtracted from the cDNA in the pituitary glands of laying geese (forward subtraction); the reverse subtraction was also performed. After sequencing and annotation, a total of 30 and 24 up and down-regulated genes were obtained from the forward and reverse SSH libraries, respectively. These genes mostly related to biosynthetic process, cellular nitrogen compound metabolic process, transport, cell differentiation, cellular protein modification process, signal transduction, small molecule metabolic process. Furthermore, eleven genes were selected for further analyses by quantitative real-time PCR (qRT-PCR). The qRT-PCR results for the most part were consistent with the SSH results. Among these genes, Synaptotagmin-1 (SYT1) and Stathmin-2 (STMN2) were substantially over-expressed in laying period compared to ceased period. These results could serve as an important reference for elucidating the molecular mechanism of higher laying performance in Huoyan geese.

성견에서 전자력을 이용한 간헐적 교정력에 의한 치아이동시 초기 조직학적 변화에 관한 연구 (A STUDY ON THE INITIAL TISSUE CHANGES TO INTERMITTENNT FORCE IN DOGS)

  • 손흥범;박영철
    • 대한치과교정학회지
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    • 제20권2호
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    • pp.319-331
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    • 1990
  • The purpose of this study was to help examining force system, optimal treatment, and prevention of relapse. Orthodontic force was generated by electromagnets, therefore duration could be freely controlled, and applied for 4 days in dogs. Force magnitude was 200gm and 50gm. duration was contious and intermittent. Intermittent duration was divided into 2 kinds of types in on/off, 1 mininute/1 minute, 10 seconds/1 minute. The results were as follows: 1. In the intermittent group to which force was applied for 10 seconds and ceased for 1 minute, osteoclast was not observed. 2. In the intermittent group to which 200gm of force was applied for 1 minute and ceased for 1 minute, there was blood circulation in periodontal space on pressure side, but mild hyalinized zone was observed. 3. More number of total osteoclast was counted in the pressure side of continous group to which 200gm of force was applied than in any other groups. 4. More number of frontal osteoclast was counted in the pressure side of intermittent group to which 200gm was applied for 1 minute and ceased for 1 minute than in any other groups. 5. On tension side, less periodontal space widening, more new bone and secondary cementum formation were observed in the intermittent group to which force was applied for 1 minute and ceased for 1 minute than in the continous group.

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학업을 중단한 경험이 있는 청소년의 성별 우울 관련요인 (Factors related to Depression according to Gender among Adolescents Who Have Ceased Attending School)

  • 이지선;도경아
    • 한국학교보건학회지
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    • 제34권2호
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    • pp.123-132
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    • 2021
  • Purpose: Adolescents are vulnerable to depression; however, many health policies for adolescents tend to target students in schools. This study aims to identify factors related to depression according to gender among adolescents who have ceased attending school either temporarily or permanently. Methods: The data were generated from the 5th Dropout Youth Panel Survey (2017), and this study included 318 students in the survey that had dropped out of school. The data were analyzed using hierarchical multiple linear regression to identify related factors in depression among the participants. The analyses were performed by SPSS 25.0 program. Results: The depression scores of the students who had ceased attending school were: 20.28±5.47 for boys; 21.23±5.88 for girls. Their depression scores are significantly associated with self-esteem (p<.001 for boys; p=.001 for girls) and social stigma (p=.002 for boys; p=.002 for girls). Among those, peer attachment (p=.050), community integration (p=.004), and community disorder (p<.001) were significantly associated with depression only in boys. Conclusion: The findings of this study suggest that strategies for managing depression in adolescents who have dropped out of school should address the differences in contributing factors according to gender. This study also suggests a basis for approaching such a strategy.

Progesterone 농도측정(濃度測定)에 의한 유우(乳牛)의 번식효율증진(繁殖效率增進)에 관한 연구(硏究) IV. 유즙(乳汁)중 progesterone 농도측정(濃度測定)에 의한 분만후(分娩後) 난소기능(卵巢機能) 회복상태(回復狀態)의 검토(檢討) (Progesterone assays as an aid for improving reproductive efficiency in dairy cattle IV. Milk progesterone profiles for monitoring postpartum ovarian activity)

  • 강병규;최한선;최상공;손창호;강현구
    • 대한수의학회지
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    • 제34권4호
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    • pp.881-890
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    • 1994
  • Skim milk progesterone($P_4$) profiles in 74 dairy cows were determind to monitor postpartum ovarian activity by radioimmunoassay. Milk samples were collected from each cow every 5 days from 10 to 90 days postpartum. Signs of estrus were observed twice daily, and status of the ovaries and uterus were examined every 10 days by rectal palpation. Results are summarized as follows: 1. Cows were categorized into five types by the change of skim milk $P_4$ profiles; Type I(normal) : Cyclic changes of skim milk $P_4$ profiles appeared within 20 days postpartum(12 cows, 16.2%), Type II(cycle delayed) : Cyclic changes of skim milk $P_4$ profiles appeared from 21 to 60 days postpartum(39 cows, 52.7%), Type III(cycle ceased with low $P_4$) : Onset of the estrous cycle within 20 days postpartum but ceased later with low levels of $P_4$ (7cows, 9.5%), Type IV(cycle ceased with high $P_4$) : Onset of the estrous cycle within 20 days postpartum but ceased later with high levels(>3.0 ng/ml) of skim milk $P_4$ (4 cows, 5.4%), Type V(acyclicity) : Skim milk $P_4$ concentration remained low(<1.0 ng/ml) until 80 days postpartum(12 cows, 16.2%). 2. Out of the 17 cows classified as the Type III and Type V by skim milk $P_4$ profiles, 13 cows had inactive ovaries and remaining 6 cows had single or multiple follicular cysts in their ovaries by rectal palpation. All 4 cows of Type IV had a persistent corpus luteum in their ovaries. 3. Approximately eighty percent of the cows had begun ovarian activity by 60 days postpartum and 90.6% by 90 days by skim milk $P_4$ profiles, but only 39.2% by 60 days and 71.7% by 90 days had shown visible estrus signs. The mean days from parturition to the first, second and third ovulations determined by skim milk $P_4$ profiles was $28.0{\pm}11.0$, $46.4{\pm}13.3$ and $66.4{\pm}11.5$ days and the visible estrus signs were 9.3%, 38.1%, and 48.6%, respectively. The mean days from parturition to the first visible estrus was $57.2{\pm}15.9$ days. These results indicated that milk $P_4$ profile of each Types by radioimmunoassay can be utilized for monitoring postpartum ovarian and would be useful for the early detection of ovarian dysfunction in dairy cow.

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치아경조직의 약물 침투에 관한 연구 (Study for Medicinal Penetration in Dental Tissue)

  • 양동규
    • 대한치과의사협회지
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    • 제11권4호
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    • pp.271-276
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    • 1973
  • This study is concerned with the effect on Penetration of varing the length of time among the applications of stains, preparations of medicine and radio-active isotope of siver in 120 carious or non carious human teeth with a vital pulp. The study revealed the following conclusions : 1) The stains and preparations penetrated through the dentinal tube and it's seem to be ceased at zone of secondary dentinal area. 2) The stains and preparations did not penetrated in enamel tissues.

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계단식 지오그리드 보강토 옹벽의 계측 (Instrumentation of A Two-Level of Soil-Reinforced Segmental Retaining Wall)

  • 유충식;정혁상
    • 한국지반공학회:학술대회논문집
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    • 한국지반공학회 2002년도 가을 학술발표회 논문집
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    • pp.697-704
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    • 2002
  • This paper presents the results of instrumentation of a two-level of soil-reinforced segmental retaining wall. Instrumentation items include the lateral wall displacements and the geogrid strains at several locations. The instrumentation is still long carried in order to examine long-term behavior. The result indicate that the upper wall has a significant effect on the behavior of the lower wall doubling the wall moved. The wall also exhibits significant post-construction movements that had ceased several months after the wall completed. The implication of the findings from this study was discussed in great detail.

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고방(古方)을 통한 급성 폐쇄성 녹내장의 한방치험 1례 (A Case Report of Acute Angle Closure Glaucoma Patient Treated with Go-bang)

  • 한기은;강은정
    • 대한한의학회지
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    • 제44권2호
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    • pp.170-180
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    • 2023
  • Objectives: This is a case report on the treatment of one patient with acute angle-closure glaucoma using traditional Korean medicine. Methode: We assessed the progression of acute angle-closure glaucoma by analyzing Optical Coherence Tomography(OCT) results. Results: Acute angle closure glaucoma did not progress to OCT after treatment. Conculsion: We obtained results from OCT examinations indicating that the progression of acute angle-closure glaucoma had ceased after Korean herbal medicine treatment.

심해저 망간단괴 생산규칙의 잠재적 환경보호 의무 범위에 관한 연구 (The Scope of Potential Duties for Environment Protection in the Regulation on the Exploitation for Polymetalic Nodules in the Area)

  • 김정은;박성욱
    • Ocean and Polar Research
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    • 제37권1호
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    • pp.81-90
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    • 2015
  • International Seabed Authority (ISA) is currently developing rules with regard to exploitation of manganese nodules which will be contained in its first regulations governing the exploitation of deep seabed mineral resources. A stakeholder survey was conducted in the early 2014 by ISA with the aim of facilitating participation of interested entities in the development process of the rules. The stakeholders who had replied to the survey included existing contractors, sponsoring States, environmentalists, academics, and nongovernmental organizations. Opinions given by them largely reflect their own interests. This paper aims to clarify the scope of the obligations regarding the environmental protection which may be imposed on contractors under the new regulations for the exploitation of manganese nodules. To do so, it first analyses the express provisions on environmental protection applicable to deep seabed mining included in the Law of the Sea Convention, its agreement on implementation of Part XI, and the regulations on exploration for manganese nodules. Secondly, it categorizes these obligations based on the categories of international obligations suggested by Combacau and Alland. Based on the categorizations this paper concludes that, in addition to the existing duties to protect deep seabed environment within the Law of the Sea Convention system, the following new obligations could be added: conservation of exploitation sites for a limited time after the contract is ceased; taking all necessary measures for rehabilitation of destroyed ecosystems that occurredas a result of mining activities; monitoring exploitation sites for a limited period time after the contract is ceased; observing rules and standards on safety of ships and environmental protection adopted under IMO instruments; regulation on the discharge of mine tailings from the facilities used for exploitation of deep sea minerals. Lastly, this paper attempts to provide ways of reflecting national interests in terms of potential obligations which may be included in the new regulations.

아연화의 최적 합성조건 (Optimal Synthesis Conditions of Zinc White)

  • 신화우;김윤설
    • 약학회지
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    • 제40권6호
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    • pp.659-665
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    • 1996
  • Zinc white is mainly used as a mild astringent, protectant. and has weak antiseptic action. It is well known that the yield of zinc white produced is greatly affected by the syn thetic conditions such as the reactant concentration, reaction temperature, washing water temperature, mole ratio of reactants, and drying temperature, calcination temperature, etc. The purpose of this study is to investigate the optimal synthesis conditions of zinc white produced. A randomized complete block design suggested by G.E.P. Box and K.B. Wilson was applied for this purpose. Basic zinc carbonate was prepared by reacting zinc sulfate and sod. carbonate solution in this study. Zinc white comes when prepared by calcination of basic zinc carbonate. The optimum synthesis conditions of zinc white obtained from this study is as follows: 1) The reacting temperature range is: 92-100$^{\circ}C$, 2) The concentration of reactant solution is 23.6-27%, 3) The optimum mole-ratio: [ZnSO4]/[Na2CO3] is 1.74~1.96, 4) The washing water temperature is 36$^{\circ}C$, 5) The drying temperature range is 68-74$^{\circ}C$, 6) The calcination temperature is 600$^{\circ}C$. The outcome of DSC indicated a desolvation of basic zinc carbonate occurred at about 133.3$^{\circ}C$. The dehydration of the compound ceased at about 267.9$^{\circ}C$ and the decarboxylation ceased at about 379.9$^{\circ}C$. The physical and chemical properties of zinc white as medicine were studied by use of Volume Test.

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춘천지역에서 생장하는 주요 수종의 연륜형성(I) - 형성층 활동기간에 관하여 - (Annual Ring Formation of Major Wood Species Growing in Chuncheon, Korea(I) - The Period of Cambium Activity -)

  • 권성민;김남훈
    • Journal of the Korean Wood Science and Technology
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    • 제33권4호통권132호
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    • pp.1-8
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    • 2005
  • 춘천지역에서 생장하는 주요수종의 생장특성을 규명하기 위해 형성층 분열기 및 형성층의 휴지기를 조사하였다. 소나무와 잣나무는 3월 중순~하순에 형성층 활동이 시작되었다. 5월 하순에서 7월 중순 사이에 형성층 세포의 분열이 활발하였고, 이 때 잣나무의 세포 수가 소나무의 세포 수보다 크게 증가하였다. 환공재인 신갈나무와 아까시나무의 형성층 분열은 3월 중순경에 개시되었으며 동시에 도관 형성이 시작되었다. 산공재인 층층나무와 산벚나무의 형성층은 4월 초에 분열을 개시하였고 도관은 이보다 1~3주일 늦게 형성되는 것으로 관찰되었다. 형성층 활동이 멈추는 시기는 침엽수 11월 초순경, 활엽수 10월 중순경으로 관찰되었다. 따라서 형성층의 활동은 동일한 환경조건에서도 수종간에 차이가 있는 것으로 나타났다.