• Journal of Physiology & Pathology in Korean Medicine
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• v.25 no.4
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• pp.641-649
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• 2011

This study was purposed to evaluate the effects of root of Liriope spicata (RLS) on LPS-induced COPD (chronic obstructive pulmonary disease) model. The extract of RLS was treated to A549 cells and LPS-induced COPD mice model. Then, various parameters such as cell-based cyto-protective activity and histopathological finding were analyzed. RLS showed a protective effect on LPS-induced cytotoxicity in A549 cells. This effect was correlated with analysis for caspase 3 levels, protein level of cyclin B1, Cdc2, and phospho-Erk1/2, and gene expression of TNF-${\alpha}$ and IL-$1{\beta}$ in A549 cells. RLS treatment also revealed the protective effect on LPS-induced lung injury in COPD mice model. This effect was evidenced via histopathological finding including immunofluence stains against caspase 3, and protein level of cyclin B1, Cdc2, and Erk1/2 in lung tissue. These data suggest that RLS has a pharmaceutical properties on lung injury. This study would provide an scientific evidence for the efficacy of RLS for clinical application to patients with COPD.

• Korean Management Science Review
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• v.2
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• pp.59-71
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• 1985

선형계획법은 경영과학 (Operations Research)에 있어서 가장 널리 활용되고 있는 모형중에 하나이다. 그래서 선형 계획법과 같이 깊이 연구된 모형도 없을 것이며 이 모형과 같이 다양한 전산프로그램이 있는 모형도 없을 것이다. 전산기 제조회사는 거의 모두 자체 선형계획법용 프로그램을 제공하고 있으며 예를 들면 IBM MPSX/370, CDC APEX III, UNIVAC MPS, HONEYWELL ALPS, FACOM LIPS 등이 있으며 전산기 제조회사가 아니더라도 독자적으로 선형계획법 프로그램을 개발하여 제공하는 곳도 있다.(중략)

• Computational Structural Engineering
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• v.4 no.3
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• pp.40-43
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• 1991

한국과학기술연구원(KIST)부설 시스템공학연구소(SERI)가 보유하여 일반 사용자들에게 공개하고 있는 구조해석 S/W/로는 ABAQUS, ADINA, BOPACE-3D, DYNA-3D, MSC/NASTRAN, DIS/ADLPIPE, SAP5, KISTRAS, NONSAP, MARC 등을 들 수 있다. 이들 중 DIS/ADLPIPE, SAP5, KISTRAS, NONSAP은 CDC CYBER 960-31에 설치되어 있고 MARC는 NAS AS/XLV50에 설치되어 있으며 나머지는 CRAY-25 슈퍼컴퓨터에 설치되어 있다. 본 고에서는 이들 중에서 CRAY-2S 슈퍼컴퓨터에 설치되어 있으며 다양한 비선형 구조해석기능을 가지고 있는 ABAQUS, ADINA, MSC/NASTRAN 및 DYNA-3D에 대하여 개요, 기능 및 사용방법을 간략히 소개하고저 한다.

• Proceedings of the Korean Society of Toxicology Conference
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• 2002.05a
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• pp.83-83
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• 2002

To assess the role of 8-oxoguanine glycosylase (OGG1) in the cell defense against radiation injury, the radiation-induced cytotoxicities were compared between the mutant type KG-1 featuring a loss of OGG1 activity due to a homozygous mutation of Arg 229 G1n, and the wild type U937.(omitted)

• Journal of the korean academy of Pediatric Dentistry
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• v.44 no.2
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• pp.170-179
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• 2017

The purpose of this study was to compare the cariogenicity of commercially available bovine milk and low-fat milk in a biofilm model using the CDC Biofilm Reactor. Streptococcus mutans ATCC 25175 biofilms were formed on saliva coated bovine enamel slabs in a CDC Biofilm Reactor. Biofilms were exposed three times per day to one of the following materials: commercial whole milk (fat content: 3.4%), low-fat milk (fat content: 1%), or 0.9% NaCl. Medium pH was measured at different time points. After 5 days, biofilms were separated from slabs to evaluate the CFUs. The biofilm thickness was observed by confocal laser-scanning microscopy (CLSM). Enamel slab's demineralization was assessed by measuring surface microhardness before and after the experiment. For microhardness and CFUs assessment, no significant difference was found among the three groups. All groups showed similar pattern of medium pH change and biofilm thickness. Our results showed that there was no difference in the cariogenicity between whole milk and low-fat milk. Both milks were relatively non-cariogenic compared to the control group.

• Genomics & Informatics
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• v.21 no.2
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• pp.20.1-20.10
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• 2023

Aromatase inhibitors (AI) are drugs that are widely used in treating estrogen receptor (ER)-positive breast cancer patients. Drug resistance is a major obstacle to aromatase inhibition therapy. There are diverse reasons behind acquired AI resistance. This study aims at identifying the plausible cause of acquired AI resistance in patients administered with non-steroidal AIs (anastrozole and letrozole). We used genomic, transcriptomic, epigenetic, and mutation data of breast invasive carcinoma from The Cancer Genomic Atlas database. The data was then separated into sensitive and resistant sets based on patients' responsiveness to the non-steroidal AIs. A sensitive set of 150 patients and a resistant set of 172 patients were included for the study. These data were collectively analyzed to probe into the factors that might be responsible for AI resistance. We identified 17 differentially regulated genes (DEGs) among the two groups. Then, methylation, mutation, miRNA, copy number variation, and pathway analyses were performed for these DEGs. The top mutated genes (FGFR3, CDKN2A, RNF208, MAPK4, MAPK15, HSD3B1, CRYBB2, CDC20B, TP53TG5, and MAPK8IP3) were predicted. We also identified a key miRNA - hsa-mir-1264 regulating the expression of CDC20B. Pathway analysis revealed HSD3B1 to be involved in estrogen biosynthesis. This study reveals the involvement of key genes that might be associated with the development of AI resistance in ER-positive breast cancers and hence may act as a potential prognostic and diagnostic biomarker for these patients.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.11
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• pp.6419-6425
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• 2013

Background: Neoadjuvant chemotherapy (NACT) is a treatment modality whereby chemotherapy is used as the initial treatment of HNSCC in patients presenting with advanced cancer that cannot be treated by other means. It leads to shrinkage of tumours to an operable size without significant compromise to essential oro-facial organs of the patients. The molecular mechanisms behind shrinkage due to NACT is not well elucidated. Materials and Methods: Eleven pairs of primary HNSCCs and adjacent normal epithelium, before and after chemotherapy were screened for cell proliferation and apoptosis. This was followed by immunohistochemical analysis of some cell cycle (LIMD1, RBSP3, CDC25A, CCND1, cMYC, RB, pRB), DNA repair (MLH1, p53) and apoptosis (BAX, BCL2) associated proteins in the same set of samples. Results: Significant decrease in proliferation index and increase in apoptotic index was observed in post-therapy tumors compared to pre-therapy. Increase in the RB/pRB ratio, along with higher expression of RBSP3 and LIMD1 and lower expression of cMYC were observed in post-therapy tumours, while CCND1 and CDC25A remained unchanged. While MLH1 remained unchanged, p53 showed higher expression in post-therapy tumors, indicating inhibition of cell proliferation and induction of apoptosis. Increase in the BAX/BCL2 ratio was observed in post-therapy tumours, indicating up-regulation of apoptosis in response to therapy. Conclusions: Thus, modulation of the G1/S cell cycle regulatory proteins and apoptosis associated proteins might play an important role in tumour shrinkage due to NACT.

• The Korean Journal of Mycology
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• v.26 no.3 s.86
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• pp.314-320
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• 1998

A 0.4 kb cDNA fragment was isolated from mRNA of UV or mechanical wound damaged Pleurotus sajor-caju by the differential display method. Expression of the gene corresponding to this cDNA fragment was highly induced by mechanical wound damage or UV treatment. This gene showed only basal level expression in mycelia, stipe, and cap under normal growth conditions. Sequencing analysis showed that this cDNA fragment contains partial open reading frame. Homology search using genbank database revealed that although this gene do not have homology with already reported wound induced genes, it has a significant sequence homology in defined region with the cdc2-related protein kinase gene which is known to be involved in negative regulation of meiotic maturation in Xenopus oocytes.

• Journal of the Korean Society of School Health
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• v.21 no.2
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• pp.119-134
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• 2008

Purpose: The purpose of this study is to understand a variety of intervention studies to prevent smoking by adolescents in U.S. and find out implications for Korea. Methods: This study reviewed articles found in the internet and analysed the data of US DHHS and CDC. Results: The School Health Policies and Programs Study (SHPPS) is a national survey periodically conducted to assess school health policies and programs of U.S. The main components of SHPPS are health education and physical education, health services, mental health and social services, school policies, and school environments. The CDC guidelines for school health programs to prevent tobacco use and addiction are composed of policy, instruction, curriculum, training, family involvement, tobacco-use cessation efforts, and evaluation. School-based interventions to prevent smoking can be classified into the categories of information-giving curricula, social competence curricula, social influence approaches, combined methods draw on social competence and social influence approaches and multi-modal programmes and Youth Empowerment study. The key programs for adolescent smoking prevention are ALERT Project, HSPP, TNT Project, MPP, NC YES. Conclusions: As smoking is often the first step of unhealthy behaviour such as alcohol drinking, illegal drugs, and violence, smoking prevention programmes for adolescents in U.S. have been comprehensive school-based health programs. In smoking prevention programs for adolescents, CDC plays a critical role by supporting survey, research, policy, and funds. The effectiveness of the programs was high when it was based on school and involved parents, community, and mass media. As the effect of each programme is not expected to last for a long time, consistent repetition of these interventions is essential. Current smoking prevention programs for adolescents are exploring the empowerment approach focused on the active involvement of participants rather than traditional approaches using order and discipline.

• Parasites, Hosts and Diseases
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• v.57 no.2
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• pp.185-189
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• 2019

To identify the component(s) involved in cell cycle control in the protozoan Giardia lamblia, cells arrested at the G1/S- or G2-phase by treatment with nocodazole and aphidicolin were prepared from the synchronized cell cultures. RNA-sequencing analysis of the 2 stages of Giardia cell cycle identified several cell cycle genes that were up-regulated at the G2-phase. Transcriptome analysis of cells in 2 distinct cell cycle stages of G. lamblia confirmed previously reported components of cell cycle (PcnA, cyclin B, and CDK) and identified additional cell cycle components (NEKs, Mad2, spindle pole protein, and CDC14A). This result indicates that the cell cycle machinery operates in this protozoan, one of the earliest diverging eukaryotic lineages.