• Journal of Dental Rehabilitation and Applied Science
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• v.40 no.1
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• pp.1-12
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• 2024

Purpose: To histologically evaluate the effects of three calcium silicate-based sealers on rat mandible tissue. Materials and Methods: Rats were randomly divided as follows: A group that sacrificed immediately after cavity preparation, a group that sacrificed two weeks after cavity preparation, a group that sacrificed two weeks after CeraSeal (CS), AH Plus Bioceramic (AHB), or One-Fil (OF) sealer injection, respectively. After tissue processing for all groups, the bone tissue area (%) and the number of osteoclasts in and around the cavity were measured under a microscope. The results of each group were compared and statistical analysis was performed using one-way ANOVA and Tukey's test. Results: The formation of bone tissue and the presence of osteoclasts in the cavity were observed in the group that sacrificed two weeks after cavity preparation and the group sacrificed two weeks after AHB sealer injection, and these groups showed significantly higher average bone tissue area (%) than the other groups. In the other groups, no inflammation or foreign body reaction occurred in the cavity, and no osteoclasts were observed. Conclusion: All calcium silicate-based sealers used in this study showed a favorable bone tissue response when injected into the rat mandible. In particular, higher bone formation in the cavity was observed in AHB.

• Journal of the korean academy of Pediatric Dentistry
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• v.35 no.1
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• pp.11-17
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• 2008

The purpose of this study was to investigate early histological changes and calcitonin gene-related peptide (CGRP) expression in the dental pulp of the rat after Er:YAG laser preparation. Occlusal cavities were prepared in the upper first molars using either Er:YAG laser and conventional bur. At 48 hours after cavity preparation, the teeth were processed for hematoxylin-eosin stain and CGRP immunohistochemistry. The results were as follows : 1. The cavity floor by Er:YAG laser preparation was more irregular shape compared with those by bur preparation and there are some cracks in the directions of dentinal tubules. 2. There were more inflammatory cell infiltration and disruption of odontoblast in the dental pulp by Er:YAG laser preparation in comparison with the dental pulp by bur preparation. 3. CGRP expression in the pulp tissue by both Er:YAG laser and bur preparations were increased and higher than in the normal pulp. The expression pattern of CGRP was more strong in the pulp by Er:YAG laser preparation. These results indicate that Er:YAG laser is useful in the operative dentistry such as caries removal and cavity preparation if properly applied.

• Restorative Dentistry and Endodontics
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• v.21 no.2
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• pp.451-469
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• 1996

The purpose of this study was to investigate the effect of calcium hydroxide and glass ionomer cement fillings on the levels of $LTB_4$ and $LTC_4$ in experimentally inflamed rat dental pulp. The dental pulp in the mandibular incisor of wistar rat was irritated by cutting a 5mm deep hole in the dentin with a twist drill bur of 0.5mm diameter, without cooling. The cavities were filled with calcium hydroxide(light-cured) and glass ionomer cement(light cured). The untreated pulp served as control tissue specimen. After cavity preparations, the rat with or without various treatment were sacrificed in various time by decapitation. The dental pulp tissue were carefully removed and the concentrations of $LTB_4$ and $LTC_4$ were determined by radioimmunoassay. And pulps were examined histologically to observe inflammatory feature. The result were obtained as follows : 1. The inflammatory features of pulps were observed microscopically in all experimental groups. And degree of inflammation was decreased with time. 2. The concentrations of $LTB_4$ and $LTC_4$ for all experimental groups were significantly higher than those for control group 6 hours after cavity preparation(p<0.05). 3. The group filled with calcium hydroxide was the lowest, and the group filled with glass ionomer cement, the group of irritation in that order showed increased concentrations of $LTB_4$ and $LTC_4$ 6 hours after cavity preparation. In the concentrations of $LTB_4$, significant differences among 3 groups were noted(p<0.05). 4. The group filled with calcium hydroxide was the lowest, and the group filled with glass ionomer cement, the group of irritation in that order showed increased concentrations of $LTB_4$ and $LTC_4$ 24 hours after cavity preparation. And there were statistically significant difference in concentrations of $LTB_4$ between the group of irritation and the group filled with calcium hydroxide(p<0.05). 5. The group filled with calcium hydroxide was the lowest, and the group filled with glass ionomer cement, the group of irritation in that order showed increased concentrations of $LTB_4$ and $LTC_4$ 48 hours after cavity preparation. But no statistically difference was found (p>0.05). 6. The concentrations of $LTB_4$ and $LTC_4$ in all experimental groups were highest level at 6 hour after experiment and decreased as time progresses(correlation coefficient>0.8).

• Restorative Dentistry and Endodontics
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• v.23 no.1
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• pp.296-303
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• 1998

The purpose of this study was to assess the effects of cavity designs on the marginal leakage of class 5 glass ionomer restorations. The five cavity designs were as follows ; notch shape(A group), notch shape with groove(B group), combined lesion(C group), combined lesion with groove and deep chamfer margin(D group) and combined lesion with groove and shoulder(E group), and each design had 10 cavities. After the cavities were restored with GIC, they were immersed in 0.5% basic fuchsin solution for 6 hours. The specimens were washed thoroughly and sectioned longitudinally in a buccolingual direction through the center of the restorations. The degree of marginal leakage was measured as the extent of dye penetration under the stereoscope. The results of this study were as follows 1. The enamel margins of all groups showed lesser leakage than dentin/cementum margins(p<0.05). 2. The combined lesion(C group) showed more leakage than notch shape(A group), but there was no siginificant difference(p>0.05). 3. In the notch shape, there was no influence on the marginal leakage by the groove preparation. 4. In the combined lesion, marginal leakage was decreased by the groove preparation and marginal modification.

• Restorative Dentistry and Endodontics
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• v.23 no.2
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• pp.537-549
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• 1998

The purpose of this study was to evaluate the influence of root resection and retrograde cavity preparation methods on the apical leakage in endodontic surgery. To investigate the effect of various root resection and retrograde cavity preparation methods on the apical leakage, 71 roots of extracted human maxillary anterior teeth and 44 mesiobuccal roots of extracted human maxillary first molars were used. Root canals of the all the specimens were prepared with step-back technique and filled with gutta-percha by lateral condensation method. Three millimeters of each root was resected at a 45 degree angle or perpendicular to the long axis of the tooth according to the groups. Retrograde cavities were prepared with ultrasonic instruments or a slow-speed round bur, and occlusal access cavities were filled with zinc oxide eugenol cement. Three coats of clear nail polish were placed on the lateral and coronal surfaces of the specimens except the apical cut one millimeter. All the specimens were immerged in 2% methylene blue solution for 7 days in an incubator at $37^{\circ}C$. The teeth were dissolved in 14 ml of 35% nitric acid solution and the dye present within the root canal system was returned to solution. The leakage of dye was quantitatively measured via spectrophotometric method. The obtained data were analysed statistically using two-way ANOVA and Duncans Multiple Range Test. The results were as follows: 1. No statistically significant difference was observed between ultrasonic retrograde cavity preparation method and slow-speed round bur technique, without apical bevel (p>0.05). 2. Ultrasonic retrograde preparation method showed significantly less apical leakage than slow-speed round bur technique, with bevel (p<0.0001). 3. No statistically significant difference was found between beveled resected root surface and non-beveled resected root surface, with ultrasonic technique (p>0.05). 4. Non-beveled resected root surface showed significantly less apical leakage than beveled resected root surface, with slow-speed round bur technique (p<0.0001). 5. No statistically significant difference in apical leakage was found between the group of retrograde cavity prepared parallel to the long axis of the tooth and the group of one prepared perpendicular to the long axis of the tooth (p>0.05). 6. Regarding isthmus preparation, ultrasonic retrograde preparation method showed significantly less apical leakage than slow-speed round bur technique, in the mesiobuccal root of maxillary molar, without bevel (p<0.0001).

• Restorative Dentistry and Endodontics
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• v.23 no.1
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• pp.54-67
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• 1998

The purposes of this study were to evaluate the efficiency of dentin cutting and root-end cavity preparation, and to determine the incidence of tooth crack when root-end retrograde cavity preparation was done with. ultrasonic diamond instruments. To evaluate the efficiency of dentin cutting, ultrasonic diamond and stainless steel instruments were applied to 20 exposed bovine dentin surfaces perpendicularly or parallely at the low, and medium power settings for 1 minute ($Miniendo^{TM}$, EIE, CA, U.S.A.). The resultant cavity depth was measured. To evaluate the efficiency of cavity preparation and to investigate the incidence of tooth crack, 165 mesiobuccal, distobuccal and palatal root-ends of extracted human maxillary first molars were resected by 3 mm perpendicularly to the long axis of tooth using a slow speed diamond saw after root canal preparation and filling. Retrocavities were prepared using a ultrasonic diamond instrument or a stainless steel one of the low- or medium power settings of 2 or 6. Time consumed and the number of strokes used for the cavity preparation were measured and the incidence of tooth cracks was evaluated under a stereomicroscope. The results were as follows: Both at the low and medium power settings, and both with perpendicularly- and parallely applied tips to dentin, diamond instruments showed higher dentin cutting efficiency than stainless steel ones did (p<0.01). When tips were applied to dentin perpendicularly, both diamond instrument and stainless steel one showed higher cutting efficiency with medium power setting than with low power one (p<0.01). Both at the low- and medium power settings, both diamond instrument and stainless steel one showed higher cutting efficiency when tips were applied perpendicularly to dentin surface than applied parallely (p<0.01). At the medium power setting, the number of stroke and time consumed were less with diamond instrument than with stainless steel one (p<0.05) for the retrograde cavity preparation. At the low power setting, diamond instrument induced less tooth cracks than stainless steel one did (p<0.01).

• Restorative Dentistry and Endodontics
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• v.18 no.2
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• pp.395-411
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• 1993

The purpose of this study was to examine the clear concept of the designs for cavity preparations. Among the several parameters in cavity designs, profound understanding of isthmus width factor would facilitate selection of the appropriate cavity preparation for a specific clinical situation. In this study, the cavities were prepared on maxillary first premolar and filled with gold inaly. A two - dimensional model was composed of 1037 - node triangle elements. In this model, isthmus was varied in width at 1/4, 1/3 and 1/2 of intercuspal width and material properties were given for four element groups, i.e., enamel, dentin, pulp and gold. The 500N occlusal load varied in direction and it was examined using three types of load : concentrated load, divided load and distributed load. The models were also examined with empty cavities using the devided load and distributed load. These models were analyzed the displacement and strees distribution by the two - dimensional Finite Element Method. The results were as follows : 1. All experimental models which filled with gold inlay after cavity preparation were similar direction of displacement with control model under same load type. But in the models with empty cavities, as isthmus width was wider, the degree of displacement was increased at same load type. 2. Among the experimental models which were filled with gold inaly after cavity preparation, the model II showed the least stress concentration under concentrated load and divided load. But in the models with empty cavities, the model III showed the largest stress concentration and tooth fracture is expected regardless isthmus width. 3. All experimental models showed similar displacement pattern beneath restorative material under a concentrated load. In the models with empty cavities, a divided load resulted in a lingual displacement of the lingual cusp, but a distributed load resulted in a buccal displacement of the lingual cusp. In regard to the above results, the restored models were stronger than empty models in respect to the bending moment and tensile stress. The empty models are expected to fracture regardless isthmus width. The safest isthmus width was 1/3 of intercuspal distance, which showed the least stress concentration in respect to the effect of stress distribution.

• Restorative Dentistry and Endodontics
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• v.21 no.2
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• pp.619-627
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• 1996

There are increasing use of composite resin in the posterior teeth and the new indirect inlay technique was introduced for compensating much troubles faced in direct technique. Many researchers insisted that overall properties of restorative materials were enhanced by an additional curing but this technique still has a problems about using cement material. Resin inlay obtains retention force from friction and another adhesion to tooth structure. A shape of cavity preparation was noted but studies about cement thickness and bond strength with cavity divergency are rare. The purpose of this study is to assess the effect of cavity divergency on cement thickness and bond strength of resin inlay. Cavities, which divergency was $6^{\circ}$, $16^{\circ}$, and $26^{\circ}$ in each group, were prepared and their divergency was verified by Adobe Photoshop program through the image capture with stereo microscope and FlexCam. Inlays were fixed into the cavities with a resin cement, Superbond and were handled under chemical (in 75% ethanol for 24 hrs.) and thermal stress (500 cycles from $5^{\circ}$ to $55^{\circ}C$). MXT 70 (x400) was used for measuring the cement thickness and bond strength was evaluated with a universal testing machine. Following results were obtained : 1. The cement thickness in Mean (S.D.) were; 35.58 (10.31)${\mu}m$ in $6^{\circ}$ group, 35.97 (10.49)${\mu}m$ in $16^{\circ}$ group, and 41.43 (9.33)${\mu}m$ in $26^{\circ}$ group. But there was no significant difference between groups. 2. The bond strength in Mean (S.D.) were ; 33.18 (5.53)kg in $6^{\circ}$ group, 23.47 (13.40)kg in $16^{\circ}$ group, and 19.75 (10.48)kg in $26^{\circ}$ group. $6^{\circ}$ group showed significantly higher value compared to $16^{\circ}$ and $26^{\circ}$ groups (p<0.05). Although the results of this study indicate $6^{\circ}$ divergency will be good for resin inlay, cavity preparation with this type will have lots of difficulties in manufacturing, try-in, and cementation procedures, such as deformation. So it is concluded that $16^{\circ}$ divergent cavity preparation is recommended in resin inlay technique.

• The Journal of the Korean dental association
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• v.23 no.5 s.192
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• pp.367-367
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• 1985

• The Journal of the Korean dental association
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• v.23 no.4 s.191
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• pp.277-277
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• 1985