• The Korean Journal of Physiology and Pharmacology
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• 제5권6호
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• pp.503-510
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• 2001

Long-term treatment of cultured bovine adrenal medullary chromaffin (BAMC) cells with arachidonic acid $(100\;{\mu}M),$ angiotesnin II (100 nM), prostaglandin $E_2\;(PGE_2;\;10\;{\mu}M),$ veratridine $(2\;{\mu}M)$ or KCl (55 mM) for 24 hrs increased both norepinephrine and epinephrine levels in the supernatant. Pretreatment with staurosporine (10 nM), a protein kinase C (PKC) inhibitor, completely blocked increases of norepinephrine and epinephrine secretion induced by arachidonic acid, angiotensin II, $PGE_2,$ veratridine or KCl. In addition, K252a, another PKC inhibitor whose structure is similar to that of staurosporine, effectively attenuated both norepinephrine and epinephrine secretion induced by arachidonic acid. However, K252a did not affect the catecholamine secretion induced by angiotensin II, $PGE_2,$ veratridine or KCl. Our results suggest that staurosporine may inhibit long-term catecholamine secretion induced by various secretagogues in a mechanism other than inhibiting PKC signaling. Furthermore, long-term secretion of catecholamines induced by arachidonic acid may be dependent on PKC pathway.

• Nutrition Research and Practice
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• 제11권1호
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• pp.25-32
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• 2017

BACKGROUND/OBJECTIVES: Stress-induced cognitive impairment is related to the suppression of hippocampal neurogenesis that results from an increase of oxidative stress. Therefore, the aim of this study was to investigate the effects of administration of a blueberry drink, having a high antioxidant power, on the cognitive performance of adult rats exposed to chronic mild stress. MATERIALS/METHODS: Twelve-week-old male Sprague-Dawley rats (n = 48) were randomly divided into four groups: control (CO), stress (ST), control + 5% blueberry drink (CO + B), and stress + 5% blueberry drink (ST + B). After eight weeks, the cognitive performance was assessed using a multiple T-maze water test. Levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and ascorbic acid were measured in the brain, and catecholamine concentrations were measured in plasma. RESULTS: The brain weights of the rats from the ST and ST + B groups were significantly lower than those of the rats from the CO and CO + B groups. The cognitive performance of the ST group was impaired when compared to that of the CO group. This impairment was significantly improved by the blueberry drink supplementation (P < 0.05). The brain SOD and CAT concentrations were not influenced by the stress or by the blueberry drink. However, the brain levels of GPx and ascorbic acid were significantly lower in the ST group than those in the CO group and were increased by the blueberry drink supplementation. The plasma catecholamine concentrations were affected by chronic mild stress and by the blueberry drink. The plasma norepinephrine and dopamine concentrations were decreased by the chronic stress and improved by the blueberry drink supplementation. The plasma epinephrine level was only influenced by the stress. CONCLUSION: These findings suggest that the blueberry drink may protect against the cognitive impairment induced by chronic mild stress.

• Journal of Chest Surgery
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• 제31권2호
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• pp.198-202
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• 1998

악성 간엽종은 유아와 태생기에서 매우 드문 종양이다. 악성간엽종은 성인에서 극히 드물고 섬유조직이외의 둘 또는 그 이상의 간엽성분으로 구성된 종양으로 결체조직의 어떤 형태로도 분화할 가능성이 있는 태생기 간엽에서 기원한다고 생각된다. 환자는 61세 남자로 2개월 전 가래를 동반한 기침을 주소로 검사를 받던 중 우폐상부에 매우 큰 종괴를 보이는 암종으로 입원하였다. 환자는 우폐상부 미세침윤조직검사상 편평상피세포암 의심하에 우전폐절제술을 받았다. 수술장 소견상 종괴는 흉벽과 심하게 유착되었으며 우폐하부까지 침습된 소견을 보이는 악성간엽종으로 진단되었다. 환자는 수술후 5,000rad의 방사선 조사를 받고 수술후 6개월째인 현재까지 재발징후없이 경과 관찰중이다.

• Journal of Yeungnam Medical Science
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• 제40권4호
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• pp.435-441
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• 2023

Pheochromocytomas and paragangliomas (PPGLs) may secrete hormones or bioactive neuropeptides such as interleukin-6 (IL-6), which can mask the clinical manifestations of catecholamine hypersecretion. We report the case of a patient with delayed diagnosis of paraganglioma due to the development of IL-6-mediated systemic inflammatory response syndrome (SIRS). A 58-year-old woman presented with dyspnea and flank pain accompanied by SIRS and acute cardiac, kidney, and liver injuries. A left paravertebral mass was incidentally observed on abdominal computed tomography (CT). Biochemical tests revealed increased 24-hour urinary metanephrine (2.12 mg/day), plasma norepinephrine (1,588 pg/mL), plasma normetanephrine (2.27 nmol/L), and IL-6 (16.5 pg/mL) levels. ¹⁸F-fluorodeoxyglucose (FDG) positron emission tomography/CT showed increased uptake of FDG in the left paravertebral mass without metastases. The patient was finally diagnosed with functional paraganglioma crisis. The precipitating factor was unclear, but phendimetrazine tartrate, a norepinephrine-dopamine release drug that the patient regularly took, might have stimulated the paraganglioma. The patient's body temperature and blood pressure were well controlled after alpha-blocker administration, and the retroperitoneal mass was surgically resected successfully. After surgery, the patient's inflammatory, cardiac, renal, and hepatic biomarkers and catecholamine levels improved. In conclusion, our report emphasizes the importance of IL-6-producing PPGLs in the differential diagnosis of SIRS.

• Journal of Nutrition and Health
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• 제18권1호
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• pp.23-28
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• 1985

분만전후 Sprague Darwley 암컷 쥐에서 양적으로 식이를 계한하여 임의로 먹인 Control group의 일일평균섭취량이 1/2의 시중사료를 식이제한 group에게 주었다. Control group은 실험기간 동안 임의로 먹게 두었고, Deficient I group은 임신중에는 임의로 먹였고 분만후부터 이유시까지는 1일 20g의 사료를 주었다. Deficient II group은 임신 15일부터 분만시까지 1일 15g의 사료를 주었고 분만후부터 이유시까지는 1일 20g의 사료를 주었다. 각 group에서 새끼쥐가 출생한 날을 0일로 해서, 0, 3, 7, 10, 14, 21일에 새끼쥐의 serotonin, ca-techolamine 및 tryosine의 함량을 측정했다. 새끼쥐 뇌의serotonin함량은 control group에 비해 식이제한 group에서 출생 7일부터 유의적으로 낮아졌으며, 두 식이제한 group사이에서는 출생 14일과 21일에 유의적인 차를 보였다. 뇌에서 norepinephrine과 dopamine같은 catecholamine은 Control group보다 식이제한 group에서 유의적으로 낮았지만, 두 식이제한 group사이에서는 유의적 차를 보이지 않았다. Catecholamine의 전구체인 tyrosine은 식이제한 group의 뇌에서보다 Control group에서 출생 3일에 유의적으로 낮았으며, 출생 14일에는 Deficient I group의 tyrosine함량이 Control group과 Deficient II group의 뇌에서 보다 유의적으로 낮았다. 임신 마지막 주와 수유시의 어미쥐의 식이제한은 새끼쥐 뇌에서 정상적인 catecholamine합성을 지연시켜 주는 것으로 추측되며, 만일 식이제한을 시키는 기간을 이유 후로 연장시켜 주면, 뇌에서 유의적으로 낮은 catecholamine의 함량이 정상으로 회복될 수 있으리라 기대된다. 본 연구결과를 통하여 볼 때, 분란전후의 어미쥐에게 영양부족을 시키면, 새끼쥐 뇌성장발육과 조성이 정상적으로 될 수 없음을 알 수 있다.

• 한국임상약학회지
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• 제7권2호
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• pp.81-85
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• 1997

Berberine, a protoberberine isoquinoline alkaloid, showed inhibitory effects on dopamine content in PC12 cells $(53.8\%\;inhibition\;at\;20\;{\mu}M)$. Tyrosine hydroxylase, the rate-limiting enzyme in the catecholamine biosynthetic pathway, was inhibited at $20\;{\mu}M)$ of berberine by $21.8\%$ relative to control. Thus, we hypothesized that the inhibition of tyrosine hydroxylase by berberine might be partially contributed to the decrease in dopamine content in PC12 cells. Furthermore, we investigated the effects of berberine on catecholamine content of serum after immobilization and cold stress in mice. Adult male mice were either subjected to 30 min of restraint or to 2 hr of cold chamber at $4-6^{\circ}C$. Serum norepinephrine, 16.8 pmol/ml, in control mice was increased to 28.8 pmol/ml by immobilization and the stress-induced rise in serum norepinephrine was partially blocked by the treatment of berberine (10 mg/kg, i.p.) once a day for 6 days. Berberine (10 mg/kg/day for 3 days, i.p.) also inhibited the increase in serum norepinephrine by cold stress in mice. These results suggest that berberine may be developed as the promising antistress agent.

• 동의신경정신과학회지
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• 제7권1호
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• pp.39-48
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• 1996

This study was performed to investigate the effects of Qigong training after physical training stress in untrained college students For this study, 6 voluntary subjects(male 4, female 2) were chosen in untrained students of K University. they were trained by teachers during 6 weeks and tested just before Qingong training and after 6 weeks. Each subject was performed a treadmill exercise(model Q65, Quinton Co, U.S.A.) to the all-out state. During exercise stress test, electrocardiogram, heart rate were checked by stress test monitor(model Q4500, Quinton Co, U.S.A) and also oxygen uptake, maximal oxygen uptake analyzed continuously by automatic gas analysis(model QMC, Quinton Co, U.S.A). During physical training the serum were collected 3 times, pre-experimental rest time, and serum catecholamine were measured by HPLC.T-test of statistical analysis system was used in every experiment for statistical assessment. The results of T-test on these data were summarized as follow:1.Heart rate change during exercise stress test after Qigong training was shown more decreasing tendency than before training. Especially, heart rate change after Qigong training during resting periods was decreased significantly than before training.2. Oxygen uptake change during exercise stress test after Qigong training was shown more increasing tendency than before training, And also maximal oxygen uptake after Qigong training was shown more increasing tendency than before 6 weeks.3. Epinephrine level of after Qigong training was more decreased significantly than before training in all-out state. And norepinephrine level of after Qigong training was shown more decreasing tendency than before training in all-out state and after 30 minutes rest time. Above results indicate that Qigong training for 6 weeks could be effective to elevate the cardiopulmonary functions and diminish the stress responses of the physical stress.

• 대한한방내과학회지
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• 제25권4호
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• pp.52-64
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• 2004

Objectives : This study was done to investigate the effect of Gamigehyuldeung-tang on hypertension. Methods : After administering Gamigehyuldeung-tang extract to SHR(Spontaneous Hypertensive Rats) for 5 weeks, changes in blood pressure, pulse rate, aldosterone and catecholamine levels in plasma were examined, and immunohistochemical changes and scanning electron microscopic changes were observed. Results : The following results were obtained; blood presure decreased significantly as well as levels of aldosterone, dopamine and epinephrine in SHR. But levels of norepinephrine were unaffected. No capillary vessel dilation was observed. A decrease in cell damage was seen in microscope investigation. Conclusions : These results support a role for Gamigehyuldeung-tang might be usefully applied in treatment of hypertension.

• 동의생리병리학회지
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• 제20권3호
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• pp.663-669
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• 2006

This study was done to investigate the effect of Ganyangsanghang-bang(GYSHB) on hypertension. After administering GYSHB extract to SHR for 5 weeks, changes in blood pressure, pulse rate, aldosterone and catecholamine levels in plasma were examined, and immunohistochemical changes were observed, and liver function test was done. The following results were obtained; blood presure decreased significantly as well as levels of aldosterone and norepinephrine in SHR. But levels of dopamine were unaffected. No capillary vessel dilation in adrenal cortex was observed. Safety against hepatic toxicity was showed. These results support a role for GYSHB might be usefully applied in treatment of hypertension.

• 한국동물학회지
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• 제35권1호
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• pp.23-28
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• 1992

The effects of an antimetabolite, 6-aminonicotinamide (6-AU) on the levels of glucose, glycogen, catechoamines and mucleotides in mice brain were investigated. The level of glucose in the blood starts increasing from 3 h after administration of 6-AU while those in the brain tissue start increasing from 9 h after administration of 6-AN. The concentration of brain glvcogen remained unchanged at all time points except 11h. The level of epinephrine in the brain was found to reach maximum value at initial 3 h following 6-AU administration, after urhich it started dec$\ulcorner$easing si역서cantle. The Brvel of brain norepinephrine remained virtually unchanged before 24 h time point at which it starts decreasing significantly. ATP, CTP, UMP and UTP levels were significantly reduced but AMP and CMP levels urere not affected.