• Journal of the Korean Chemical Society
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• v.37 no.12
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• pp.1068-1075
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• 1993

Catalase activities were assayed on the leaves and roots of Tomato (Lycopersicum esculentum) as a function of age for 1 year after germination. The enzyme activities of root tissue demonstrated to be insignificant through all stages of development. On the other hand, the catalase activities of leaf peroxisomal fractions showed remarkable changes with peak value of 76 ${\mu}mol$/ml/min at germination stage within 2 weeks growth, 7.2 ${\mu}mol$/ml/min at adult stage around 4∼5 months growth and very small activities at aged period around 11∼12 months growth. It is suggested that there may be two factors concerning such catalase activities in relation with age, firstly the glyoxisomal reactions including glyoxylate cycle and $\beta$-oxidation at germination stage and secondly photosynthesis hereafter seemed to affect age dependent changes of catalase activity by producing coincident amount of $H_2O_2$. In addition, NADPH coenzyme was found to have ability to restore catalytic acitivity of inactivated catalase (compound II) at all stages of development except old age, indicating NADPH would play a role as catalase protector against deleterious substrate,$H_2O_2$.

• Applied Biological Chemistry
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• v.31 no.2
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• pp.205-210
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• 1988

The changes in peroxidase and catalase activities, and isoperoxidase patterns in different parts of mung bean seedling caused by the treatment with plant growth substances, $GA_3$ and ABA, were examined. As germination proceeded, the activity of peroxidase in all part except hypocotyl was increased, while that of catalase decreased. The separate application of $GA_3$ and ABA increased the activity of peroxidase which was more influenced by $GA_3$ than by ABA only in cotyledon, while that of catalase was more affected by ABA than by $GA_3$. Electrophoretic study revealed that the number of isoperoxidase was increased continuously in all parts during development. A greater influence was exerted on the intensity of isozyme than the number of isozyme by the hormonal treatment.

• Journal of radiological science and technology
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• v.15 no.1
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• pp.123-130
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• 1992

This study was prepared to observe the change of enzyme activities in kidney treated with red ginseng extract in the gamma ray irradiated mice. Determine the activity of SOD, peroxidase, catalase in the kidney a period of 1 day, 2 day, 3 day, 4 day, 5 day after a saline injection or injection of red ginseng extract or gamma ray irradiatied group into four classify. The activity SOD and catalase showed a tendency to increase and recovery at the early state but pay no regard. Wherease, the activity of peroxide restored and increased pay regard. A physiological saline injection group after gamma ray irradiation showed a tendency to diminish after remakable increase of activity of SOD, peroxidase and catalase than control group. Injection group of red ginseng extract after gamma ray irradiation observed rapid recovery on activity of SOD, peroxidase, catalase than a saline injection group. Experimental result suggested that injection of red ginseng extract after irradiation have the recovery effect on the changed of activity of SOD, peroxidase and catalase against radiation injury.

• Applied Biological Chemistry
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• v.39 no.3
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• pp.222-226
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• 1996

Lipoxygenase, peroxidase and catalase activities were determined in tissues and brines of refrigerated dill pickling cucumbers in response to pickling process, storage and $H_2O_2$. Lipoxygenase was almost inactivated within 1 day exposure to dill pickling brine, and then gradually declined during storage. In contrast, peroxidase activity in cucumber tissue decreased steadily for 4 days after exposure to dill pickling brine. Catalase was present in fresh cucumber tissues, but only slight activity was observed after submerging cucumbers in pickling brine. Lipoxygenase, peroxidase and catalase activities were rapidly inactivated in cucumbers exposed to brine containing $H_2O_2$.

• Journal of Life Science
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• v.14 no.3
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• pp.406-410
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• 2004

Glyoxal or methylglyoxal was incubated with catalase in 0.24 M sodium phosphate buffer (pH 7.4) at 37$^{\circ}C$. Dicarbonyls modify and inactivate catalase. Plasmid DNA that is directly incubated with glycation propagators, glyoxal and methylglyoxal, showed different DNA mobility shift compared to nomal plasmid DNA. When plasmid DNA is added in Fenton reaction with glycated catalase, plasmid DNA was significantly strand broken and 8-hydroxydeoxyguanosine production was time dependently increased. These results suggest that glycation of antioxidant is synergistic effect to oxidative stress.

• Current Research on Agriculture and Life Sciences
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• v.15
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• pp.93-100
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• 1997

Plants are exposed to wide range of different stresses. As plants have only limited mechanism for stress avoidance, they require flexible means for adaption to changing environmental conditions. This study was carried out to reasearch the changes of antioxidant enzymes activities as caused by water stress in four lettuceUactuca sativa) lines. Four lettuce lines exposed to water stress showed premature senescence as evidenced by the consistenent reduction in the content of total soluble protein and total lipid. Water stress also caused decreased activities of superoxide dismutase, catalase, ascorbate peroxidase, but decrease rates were different. Catalase activity was decreased much more than that of ascorbate peroxidase that suggest catalase reacted with hydrogenyperoxide directly not with ascorbate peroxidase.

• Tuberculosis and Respiratory Diseases
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• v.41 no.3
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• pp.222-230
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• 1994

Background: Paraquat, a widely used herbicide, is extremely toxic, causing multiple organ failure in humans. Paraquat especially leads to irreversible progressive pulmonary fibrosis, which is related to oxygen free radicals. However, its biochemical mechanism is not clear. Natural mechanisms that prevent damage from oxygen free radicals include changes in glutathione level, G6PDH, superoxide dismutase(SOD), catalase, and glutathione peroxidase. The authors think catalase is closely related to paraquat toxicity in the lungs Method: The effects of 3-amino-1,2,4-triazole(aminotriazole), a catalase inhibitor, on mice administered with paraquat were investigated. We studied the effects of aminotriazole on the survival of mice administered with paraquat, by comparing life spans between the group to which paraquat had been administered and the group to which a combination of paraquat and aminotriazole had been administered. We measured glutathion level, glucose 6-phosphate dehydrogenase(G6PDH), superoxide dismutase(SOD), catalase, and glutathione peroxidase(GPx) in the lung tissue of 4 groups of mice: the control group, group A(aminotriazole injected), group B(paraquat administered), group C(paraquat and aminotriazole administered). Results: The mortality of mice administered with paraquat which were treated with aminotriazole was significantly increased compared with those of mice not treated with aminotriazole. Glutathione level in group B was decreased by 20%, a significant decrease compared with the control group. However, this level was not changed by the administration of aminotriazole(group C). The activity of G6PDH in all groups was not significantly changed compared with the control group. The activities of SOD, catalase, and glutathione peroxidase(GPx) in the lung tissue were significantly decreased by paraquat administration(group B); catalase showed the largest decrease. Catalase and GPX were significantly decreased by aminotriazole treatment in mice administered with paraquat but change in SOD activity was not significant(group C). Conclusion: Decrease in catalase activity by paraquat suggests that paraquat toxicity in the lungs is closely related to catalase activity. Paraquat toxicity in mice is enhanced by aminotriazole administration, and its result is related to the decrease of catalase activity rather than glutathione level in the lungs. Production of hydroxyl radicals, the most reactive oxygen metabolite, is accelerated due to increased hydrogen peroxide by catalase inhibition and the lung damage probably results from nonspecific tissue injury of hydroxyl radicals.

• Journal of Life Science
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• v.24 no.3
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• pp.227-233
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• 2014

Effects of p-coumaric acid (p-CA), benzoic acid (BA), and salicylic acid (SA) on the activities of glutathione reductase and catalase were studied in in vitro grown tobacco plants. After culturing the tobacco plants in MS medium containing $10^{-5}$ mM of p-CA, BA, and SA, the increase in the activities of two enzymes, glutathione reductase and catalase, were compared from day 20 to day 50 day, with an interval of 10 days. The growth of the tobacco plants treated with p-CA, BA, and SA was the highest on day 50. Analysis of the effect of the three substances on the activity of glutathione reductase showed that BA and p-CA decreased the activity of the enzyme compared with a control, and SA increased the activity of the enzyme. All of them showed the highest activity on day 40. SA increased the activity of catalase, but BA and p-CA reduced the activity of this enzyme. In all the experimental groups, the activity was the highest on day 40. In conclusion, p-CA and BA appear to promote the growth of tobacco plants. The growth was the best on day 50, but the activity of the antioxidative enzyme was inhibited. On the contrary, SA seemed to inhibit the growth of the tobacco plants but to promote the activity of glutathione reductase and catalase. The growth of the plants treated with SA was best on day 40.

• Parasites, Hosts and Diseases
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• v.30 no.4
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• pp.355-358
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• 1992

In early maturation stages of Paragonimus westermani (metacercariae, 4-, 8-, 12-week old worms), activities of antioxidant enzymes, such as superoxide dismutase, catalase, peroxidase and glutathione peroxidase, were examined. Specific activity of catalase was the highest in metacercariae and decreasing with age. That of superoxide dismutase was higher in metacercariae and 4-week worms. Specific activity of peroxidase was at its peak in 4-week worms while that of glutathione peroxidase was in 8-week worms. Specific activities of all these antioxidant enzymes were decreased to their lowest in 12-week old adults.

• Applied Biological Chemistry
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• v.49 no.4
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• pp.287-292
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• 2006

The effects of cadmium on the catalase activity and isozyme patterns under light and dark conditions of rice(Oryza sativa L. cv. Dongjin) seedlings were examined. Cadmium treatment resulted in the notable enhancement of $H_2O_2$ contents in the seedling roots and leaves under light and dark conditions. The catalase isozyme patterns in the roots were different from those in the leaves, showing tissue-specific expression of the enzyme. Moreover, the expression patterns of catalase isozymes in the green seedling roots were different from those in the etiolated seedling roots following cadmium treatment. The increase of total catalase activity was about 16 times at 1 mM cadmium and marked inductions of the isozyme CAT1 and CAT2 contributed to this increase in the green seedling roots. On the other hand, in the etiolated seedling roots, total catalase activity was lower than that of control at 0.5 and 1 mM cadmium, even though catalase activity increased about 3 times at 0.1 mM cadmium. The 3 fold increase of total catalase activity was mainly due to the increase of CAT1, CAT3 and CAT4 at 0.1 mM cadmium. However, treatment with higher concentrations of cadmium decreased the activity of CAT2 and CAT4 in the etiolated roots. In the leaves, the catalase existed as three isozymes; one cationic isozyme CATc, one neutral isozyme CATn and one anionic isozyme CAT1 in the control. The isozyme patterns and total activities remained unaffected by cadmium under light and dark conditions in the seedling leaves. Taken together, it seems that cadmium-induced changes of catalase might be regulated by light in the roots, but not in the leaves.