• Journal of Microbiology and Biotechnology
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• 제20권4호
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• pp.678-682
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• 2010

The deletion of sti from the Streptomyces plasmid pIJ101 made its derivative pHZ1358 an efficient vector for gene disruption and replacement. Here, pHZ1358 was further optimized by the construction of a derivative plasmid pJTU1278, in which a cassette carrying multiple cloning sites and a lacZ selection marker were introduced for convenient plasmid construction in E. coli. In addition, the oriT region of pJTU1278 was also deleted, generating a vector (pJTU1289) that can be used specifically for PCR-targeting. The efficient usage of these vectors was demonstrated by the deletion of the gene involved in avermectin biosynthesis in S. avermitilis.

• 자원리싸이클링
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• 제15권3호
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• pp.30-37
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• 2006

최근 실내 공기질에 대한 관심이 집중되고 있으며, 본 연구에서는 자동차용 폐 라디에이터를 재활용하여 진동형 히트 파이프 (pulsating heat pipe : PHP)타입의 환기열 회수기로 제작하고, 그 성능에 대해 평가하였다. 작동유체로는 R-134a를 사용하였으며, 충전율은 내부체적비($\varphi$) 30% 및 40%로 PHP를 제작하였다. PHP의 높은 열전달 성능으로 인해 컴팩트 열교환기로 제작가능하였으며, 기존의 환기열 회수기와 대등한 성능을 보여주었다. 또한 부하변동에 따라 유닛의 개수를 증감할 수 있는 Cassette type일 뿐 아니라, 자동차용 폐 열교환기의 재활용으로 높은 경제성과 효율성이 기대된다.

• 한국정보과학회논문지:컴퓨팅의 실제 및 레터
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• 제6권3호
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• pp.299-310
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• 2000

비디오 정보를 사용자에게 서비스하기 위해서는 기존의 범용 파일 서버와는 다른 새로운 개념의 서버가 요구된다. 본 논문에서는 실시간 재생 서비스를 제공하는 개인용 VOD(Video-On-Demand) 서버를 설계하고 이를 Windows NT환경에서 구현하였음을 보인다. 제안하는 개인용 VOD 시스템은 VCR(Video Cassette Recorder) 연산의 지연시간, 사용자 접속 승인 지연시간을 줄였으며 빠른 VCR 연산으로 VOD 시스템에 접속하는 사용자에게 좀더 빠른 데이타를 전송할 수 있도록 하였다. 또한 사용자들의 접속을 원활히 하기 위하여 대화 형태의 인터페이스를 제공한다.

• 대한방사선기술학회지:방사선기술과학
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• 제2권1호
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• pp.59-69
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• 1979

For the clearer visualization of the lung apices in the routine chest P-A view, we have analysed the films at the different positions and different directions of the central X-ray beams. The brief results are as the follows. 1. Best visualization of the lung apices are made with the following position of the patient and central ray. Central ray is directed to the median sagittal plane at the level of the 5th thoracic vertebra with the palms of the hands placing at the greater trochanteric area of the both femurs. 2. The position of the sterno-clavicular joint shows no alteration between the radiographs with the central beam to the film center and to the 5th thoracic vertebra, and position of the hands at the greater trochanters or iliac crests. 3. No relationship exists between the center of the film and the position of the hand. The central beam is projected more inferiorly when the beam is centered to the film center than to the 5th thoracic vertebra. 4. The scapulae are rotated sufficiently anteriorly and more inferiorly, with placing the palms at the greater trochanters, directing central ray to the 5th thoracic vertebra, and with close contact the lung apices to the cassette.

• Natural Product Sciences
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• 제20권1호
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• pp.1-6
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• 2014

P-glycoprotein (P-gp) is a permeability glycoprotein also known as multidrug resistance protein 1 (MDR1). P-gp is an ATP-binding cassette (ABC) transporter that pumps various types of drugs out of cells. These transporters reduce the intracellular concentrations of drugs and disturb drug absorption. The Caco-2 cell permeability assay system is an effective in vitro system that predicts the intestinal absorption of drugs and the functions of enzymes and transporters. Rhodamine-123 (R-123) and digoxin are well-known P-gp substrates that have been used to determine the function of P-gp. Efflux of P-gp substrates by P-gp has been routinely evaluated. To date, a number of herbal medicines have been tested with Caco-2 cell permeability assay system to assess bioavailability. There are growing efforts to find phytochemicals that potentially regulate P-gp function. The Caco-2 cell permeability assay system is a primary strategy to search for candidates of P-gp inhibitors. In this mini review, we have summarized the P-gp modulation by herbal extracts, decoctions or single components from natural products using Caco-2 cell permeability assays. Many natural products are known to regulate P-gp and herbal medicines could be used in combination with conventional drugs to enhance bioavailability.

• 한국가시화정보학회지
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• 제14권1호
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• pp.19-26
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• 2016

Modern people spend much time at indoor space. So, People want to make better indoor air condition. But the facade of building is made of glass to be seen urbanely, the effect of solar radiation makes indoor environment worse. This study designs an open space affected by solar radiation with 4-way cassette air-conditioner. Using numerical simulation, this paper investigates thermal comfort and ventilation performance with discharge angles $30^{\circ}$ and $45^{\circ}$. To study thermal comfort, this paper studies distribution of velocity, temperature and effective draft temperature. Also, this paper introduces concept of air age to study ventilation performance. The flow influenced by solar radiation determines thermal comfort and ventilation performance in room space. This study shows that discharge angle of 45 degree has better thermal comfort and ventilation performance than that of 30 degree.

• Journal of Microbiology and Biotechnology
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• 제14권5호
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• pp.1047-1051
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• 2004

The phytopathogenic bacterium Pectobacterium chrysanthemi secretes multiple isozymes of plant cell wall disrupting enzyme such as pectate lyase and cellulase. The cel gene, existing in tandem with the pel gene, was isolated previously [10]. The role of Cel5Z and PelL1 in P. chrysanthemi PY35 pathogenicity on potato tissues was assessed by mutagenizing cloned cel gene and pel gene in tandem and recombining them with the chromosomal alleles. Strains with the Km cassette interposon in pelL1 or a double mutant showed a delay in the appearance of symptoms, suggesting that P. chrysanthemi PY35 pectate lyase PelL1 may playa minor role in soft-rot pathogenesis.

• 대한임상검사과학회지
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• 제43권2호
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• pp.68-74
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• 2011

Pseudomonas aeruginosa is an aerobic, Gram-negative, glucose-nonfermenting bacterium, which has emerged as a serious opportunistic pathogen. Recently, outbreaks of carbapenem resistant P. aeruginosa give rise to significant therapeutic challenges for treating nosocomial infections. The genes of metallo-${\beta}$-lactamase (MBL), a powerful carbapenemase, are carried as a part of the mobile gene cassettes inserted into integrons playing an important role in rapid dissemination of antibiotic resistance genes among bacterial isolates. In this study, we investigated the prevalence of integron in imipenem resistant P. aeruginosa isolates. A total of 61 consecutive, non-duplicate, and imipenem resistant P. aeruginosa strains were isolated from a university hospital in the Chungcheong province of Korea. We employed repetitive extragenic palindromic sequence-based PCR (rep-PCR) method for the selection of clonally different P. aerusinosa strains. PCR and DNA sequencing were conducted for the detection of integrons. Twenty-one clonally different P. aeruginosa strains were isolated. Only one (P28) of the strains harbored $bla_{VIM-2}$ that was found as gene cassettes in class 1 integrons. Four of 21 carbapenem resistant P. aeruginosa strains harbored class 1 integron containing aminoglycoside resistance determinant. All of the integrons detected in the study contained more than one resistance gene cassette, which can mediate resistance to multiple antibiotics. To prevent further spreading of the multi-drug resistant P. aeruginosa, conseguent monitoring and clinical polices are required.

• Journal of Microbiology and Biotechnology
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• 제14권3호
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• pp.635-638
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• 2004

An ATP-binding-cassette (ABC) transporter gene in the cephabacin biosynthetic gene cluster of Lysobacter lactamgenus was characterized. The amplified orf10 (cpbJ) gene was subcloned into pET-28a(+) vector and expressed in E. coli BL21(DE3) strain by 0.5 mM IPTG at $30^{\circ}C$. The membrane fraction of recombinant E. coli cells was separated by ultracentrifugation, and solubilized using 2.5% octyl-$\beta$-D-glucoside. Using the solubilized membrane fraction, the artificial proteoliposomes were reconstituted and analyzed for the biological activity of CpbJ protein. Upon measuring ATPase activity, the proteoliposome made from recombinant E. coli membrane proteins showed slightly higher activity than that from host E. coli membrane proteins. In the measurement of membrane transport activity, the reconstituted proteoliposome of recombinant E. coli membrane proteins exhibited higher activity when both substrates of cephalosporin C and L-Ala-L-Ser were applied, compared to the case of cephalosporin C or L-Ala-L-Ser only. It implies that the CpbJ protein is an ABC transporter secreting cephabacin antibiotics synthesized in cytoplasm.

• Asian Pacific Journal of Cancer Prevention
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• 제15권16호
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• pp.6935-6938
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• 2014

Gastric cancer (GC) is one of the most common malignancies worldwide. The ABCB1 protein, a member of the ATP-binding cassette (ABC) transporter family, encoded by the ABCB1 gene, considerably influences the distribution of drugs across cell membranes as well as multidrug resistance (MDR) of antineoplastic drugs. In contrast to the extensive knowledge on the pharmacological action of ABCB1 protein, the correlation between the clinical-pathological data and ABCB1 protein expression in patients with GC remains unclear. The aim was to investigate association between ABCB1 expression and overall survival in GC patients. Human tumor fragments from 57 GC patients were examined by immunohistochemistry assay. We observed lower survival rate of patients with GC who were positive for ABCB1 expression (p=0.030). Based on these observations, we conclude that GC patients with positive ABCB1 protein immunohistochemical expression in their tumors suffer shorter overall survival.