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http://dx.doi.org/10.4014/jmb.0910.10031

Two pHZ1358 Derivative Vectors for Efficient Gene Knockout in Streptomyces

He, Yunlong (Laboratory of Microbial Metabolism, and School of Life Sciences and Biotechnology, Shanghai Jiaotong University)
Wang, Zhijun (Laboratory of Microbial Metabolism, and School of Life Sciences and Biotechnology, Shanghai Jiaotong University)
Bai, Linquan (Laboratory of Microbial Metabolism, and School of Life Sciences and Biotechnology, Shanghai Jiaotong University)
Liang, Jingdan (Laboratory of Microbial Metabolism, and School of Life Sciences and Biotechnology, Shanghai Jiaotong University)
Zhou, Xiufen (Laboratory of Microbial Metabolism, and School of Life Sciences and Biotechnology, Shanghai Jiaotong University)
Deng, Zixin (Laboratory of Microbial Metabolism, and School of Life Sciences and Biotechnology, Shanghai Jiaotong University)

Publication Information

Journal of Microbiology and Biotechnology / v.20, no.4, 2010 , pp. 678-682 More about this Journal

Abstract

The deletion of sti from the Streptomyces plasmid pIJ101 made its derivative pHZ1358 an efficient vector for gene disruption and replacement. Here, pHZ1358 was further optimized by the construction of a derivative plasmid pJTU1278, in which a cassette carrying multiple cloning sites and a lacZ selection marker were introduced for convenient plasmid construction in E. coli. In addition, the oriT region of pJTU1278 was also deleted, generating a vector (pJTU1289) that can be used specifically for PCR-targeting. The efficient usage of these vectors was demonstrated by the deletion of the gene involved in avermectin biosynthesis in S. avermitilis.

Keywords

Shuttle vector; pHZ1358; pJTU1278; pJTU1289; conjugation; PCR-targeting;

Citations & Related Records

Times Cited By Web Of Science : 7 (Related Records In Web of Science)

Reference

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