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The Analysis of Common Metabolites of Organophosphorus Pesticides in Urine by Gas Chromatography/Mass Spectrometry

  • 박성수;표희수;이강진;박송자;박택규
    • Bulletin of the Korean Chemical Society
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    • v.19 no.1
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    • pp.45-50
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    • 1998
  • Most organophosphorus pesticides may be metabolized to yield some common phosphates in human or in animals, and these metabolites may be used as the exposure biomarkers to pesticides. In this study, we developed the extraction method of four phosphate metabolites from the spiked human urine in high recovery by the solid phase extraction with a reverse-phase cartridge (cyclohexyl silica) followed by the elution with methanol. The extracted urinary metabolites were derivatized with hexamethyldisilazane/trimethyl-chlorosilane/pyridine (2 : 1 : 10, v/v/v) and identified by gas chromatography/mass spectrometry. Calibration curve obtained from each metabolite standard using by GC/MS/SIM has shown good linearity and detection limits of metabolites were the range of 0.05-0.1 ㎍/㎖ in urine. Phenthoate, one of the organophosphorus pesticides, was orally administrated to rats. Four metabolites were detected in the rat urine. The results of this study may be applied to development of exposure biomarkers for monitoring of environmental pollutants.

Fuel Cell Powered UAV with NaBH4 as a Hydrogen Source

  • Kim, Tae-Gyu;Shim, Hyun-Chul;Kwon, Se-Jin
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2008.03a
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    • pp.579-582
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    • 2008
  • PEM Fuel cell system was designed and constructed to use as a power source of unmanned aerial vehicles(UAV) in the present study. Sodium borohydride was selected as a hydrogen source and was decomposed by catalytic hydrolysis reaction. Fuel cell system consists of a fuel cell stack, a hydrogen generation system(HGS), and power management system(PMS). HGS was composed of a catalytic reactor, micropump, fuel cartridge, and separator. Hybrid power system between lithium-polymer battery and fuel cell was developed. The fuel cell system was integrated and packaged into a blended wing-body UAV. Energy density of the total system was 1,000 $W{\cdot}hr/kg$ and high endurance more than 5 hours was accomplished in the ground tests.

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Automated Wafer Separation from the Stacked Array of Solar Cell Silicon Wafers Using Continuous Water Jet

  • Kim, Kyoung-Jin;Kim, Dong-Joo;Kwak, Ho-Sang
    • Journal of the Semiconductor & Display Technology
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    • v.9 no.2
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    • pp.21-25
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    • 2010
  • In response to the industrial needs for automated handling of very thin solar cell wafers, this paper presents the design concept for the individual wafer separation from the stacked wafers by utilizing continuous water jet. The experimental apparatus for automated wafer separation was constructed and it includes the water jet system and the microprocessor controlled wafer stack advancing system. Through a series of tests, the performance of the proposed design is quantified into the success rate of single wafer separation and the rapidity of processing wafer stack. Also, the inclination angle of wafer equipped cartridge and the water jet flowrate are found to be important parameters to be considered for process optimization. The proposed design shows the concept for fast and efficient processing of wafer separation and can be implemented in the automated manufacturing of silicon based solar cell wafers.

Static Ejection Test for Separation Analysis of 2,000lb-Class Store (2,000lb급 장착물의 분리분석을 위한 지상투하시험)

  • Byungjoon Shin;Young-hee Jo;Min-soo Kim
    • Journal of the Korea Institute of Military Science and Technology
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    • v.26 no.4
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    • pp.344-351
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    • 2023
  • Static ejection tests were conducted using the 2,000lb-Class Store to provide ejector model for the store separation simulation. In this study, static ejection test device for 2,000lb-class store was constructed and reaction force applied to store was measured over time. In addition, the trajectories of the ejected store were obtained using photogrammetry and compared with the simulations using developed ejector model. The results of the static ejection test were analyzed to determine the cartridge-orifice combination to be used for store separation. Flight tests were performed by applying the analysis results and verified that the store was safely separated from the aircraft.

Development of Analysis Method of Caffeine and Content Survey in Commercial Foods by HPLC (HPLC를 이용한 카페인의 분석법 개발 및 시판 식품중 함유량 조사)

  • Kim, Hee-Yun;Lee, Young-Ja;Hong, Ki-Hyoung;Lee, Chul-Won;Kim, Kil-Saeng;Ha, Sang-Chul
    • Korean Journal of Food Science and Technology
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    • v.31 no.6
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    • pp.1471-1476
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    • 1999
  • A simple and practical method for determination of caffeine in foods was developed. The analysis of caffeine was performed by reverse phase high performance liquid chromatography using a ${\mu}-Bondapak\;C_{18}$ column at isocratic condition with methanol-acetic acid-water(20 : 1 : 79) on UV detector at 280 nm. The clean-up and extraction of caffeine in samples were based on a simple pretreatment using a Sep-Pak $C_{18}$ cartridge. Recovery rates obtained with this method for cider, candy, cookie, milk, ice cream and persimmon leaf tea were 99.23%, 99.50%, 99.17%, 99.37%, 98.93% and 99.10% respectively. And the detection limit of caffeine was $0.1\;{\mu}g/mL$. With this method, the range of caffeine contents extracted from coffee, green tea, black tea, Oolong tea(tea bag), soft drinks, ice cream, milk and commercial confectionery were $3.38{\sim}37.50\;mg/g,\;16.30{\sim}26.10\;mg/g,\;10.80{\sim}16.65\;mg/g,\;11.25\;mg/g,\;0.06{\sim}0.11\;mg/g,\;0.04{\sim}0.44\;mg/g,\;0.04{\sim}0.39\;mg/g\;and\;0.10{\sim}1.80\;mg/g$, respectively. But caffeine was not detected in the other tea such as Acanthopanax sessiliflorum tea, Angelica gigas tea, Angelica tea, Arrow root tea, Duchu'ng tea, Dunggulle tea, Ganoerma lucidum tea, Ginger tea powder, Persimmon leaf tea, Ssanghwa tea and Cocoa mix powder.

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Isolation of an Invertebrate-type Lysozyme from the Body Wall of Spoon Worm, Urechis unicinctus (개불의 체벽으로부터 i-type 라이소자임의 정제)

  • Oh, Hye Young;Park, Nam Gyu
    • Journal of Life Science
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    • v.28 no.3
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    • pp.300-306
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    • 2018
  • Lysozymes are innate immune factors that play a critical role in the defense against pathogens in various invertebrate animals including spoon worms. In this study, an invertebrate-type lysozyme was isolated from the body wall of spoon worm, Urechis unicinctus. The acidified body wall extract was partially separated using a Sep-Pak C18 cartridge. Among the fractions, the materials that were eluted with 60% methanol/0.1% trifluoroacetic acid showed the most potent antimicrobial activity against Bacillus subtilis KCTC 1021. A series of high performance liquid chromatography (HPLC) steps were then utilized to isolate a single antimicrobial absorbance peak. The molecular weight of the antimicrobial peak was approximated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), which was approximately 13 to 14 kDa. The partial primary structure of this antimicrobial protein that was analyzed, using LC-MS/MS, was CTGGRPPTCEDYAK (1611.69 Da). Homology search of these fourteen residues, using the National Center for Biotechnology Information Basic Local Alignment Search Tool (NCBI BLAST), revealed that the isolated protein was similar to the invertebrate-type lysozymes described in other animals. Then, the antimicrobial and lysozyme enzymatic (muramidase) activities of this protein were assessed. The isolated protein possessed antimicrobial activity and potent muramidase activity, which were comparable to those of hen egg white lysozyme. Therefore, the isolated protein was designated as Urechis unicinctus invertebrate-type lysozyme from the body wall, Uu-iLysb.

Residual Characteristics of Insecticides Used for Oriental Tobacco Budworm Control of Paprika (파프리카 재배기간 중 담배나방 방제에 사용되는 살충제의 잔류특성)

  • Lee, Dong Yeol;Kim, Yeong Jin;Kim, Sang Gon;Kang, Kyu Young
    • Korean Journal of Environmental Agriculture
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    • v.32 no.1
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    • pp.84-93
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    • 2013
  • BACKGROUND: This study was carried out to investigate the residual characteristics of insecticides used for Oriental Tobacco Budworm control and to establish the recommended pre-harvest residue limit leading to contribution in safety of paprika production. METHODS AND RESULTS: The recommended Pre-Harvest Residue Limits (PHRLs) of insecticides during cultivation of paprika were calculated from residue analyses of insecticides in fruits 1, 3, 5, 7, 10, 12, 15, 18 and 21 days after treatment. Paprika samples were extracted with QuEChERS method and cleaned-up with amino propyl SPE cartridge and PSA, and insecticide residues were analyzed either by HPLC/DAD or GLC/ECD. The limits of detection were 0.01 mg/kg for 5 insecticides. Average recoveries were $81.3{\pm}1.62%$-$98.3{\pm}1.58%$ of 5 insecticides at fortification levels of 0.1 and 0.5 mg/kg. The biological half-lives of the insecticides were 8.5 days for bifenthrin, 11.8 days for chlorantraniliprole, 16.8 days for chlorfenapyr, 7.1 days for lamda-cyhalothrin and 31.3 days for methoxyfenozide at recommended dosage, respectively. CONCLUSION(S): The pre-harvest residue limits for 10 days before harvest were recommended 1.05 mg/kg, 1.41 mg/kg, 0.93 mg/kg, 2.06 mg/kg and 1.08 mg/kg as bifenthrin, chlorantraniliprole, chlorfenapyr, lamda-cyhalothrin and methoxyfenozide, respectively. This study can provide good practical measures to produce safe paprika fruit by prevention of products from exceeding of MRLs at pre-harvest stage.

A Study on the determination of Residual Antibiotics and Synthetic Antibacterial Agents in Meas (II) - Simultaneous Gas Charomatography/Mass Spectrometry Analysis of Penicillin G, Chloramphenicol and Thiamphenicol - (식육중의 잔류 항생.항균제의 검정에 관한 연구 (II) - Penicillin G , Chloramphenicol, Thiamphenicol, Gas chromatography/Mass spectrometry 동시 분석 -)

  • 류재천;양종순;서지원;김명수;박종세
    • Journal of Food Hygiene and Safety
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    • v.8 no.1
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    • pp.9-15
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    • 1993
  • In an attempt to quantitate and qualitate residual antibiotics and antibacterial agents in meat simultaneously, we studied a gas chromatography-mass spectrometry(GC/M8) analysis. For a simultaneous analysis of penicillin G, chloramphenicol and thiamphenicol in meat, a simple and rapid clean-up procedure including extraction with 0.01 M EDTA-2Na Mcilvaine buffer (pH 4.0), defatting with n-hexane, and elution with 0.01M-methanolic oxalic acid from Bond Elute $C_{18}$ cartridge, and quantitation by selected ion monitoring (SIM) mode after derivatization was performed. The recoveries (%) of penicillin G, chloramphenicol and thiamphenicol (CV, %) at 1 ppm fortification level were 63.5 (7.6), 76.3 (8.1) and 84.7 (2.0), and the detection limits of those were 0.6, 0.085 and $0.084\;\mu\textrm{g}$ beef, respectively. This method using 81M mode allows excellent detection and quantitation of residual antibiotics and antibacterial agents in meat. Moreover, confirmation by a full scan electron impact mass spectrum is possible if residual level in the sample in above 1 ppm.

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Effect of Different Dose of 310nm W Light Irradiating the Dorsal Skin on Plasma Vitamin $D_3$ Contents of Broiler Chicks (브로일러 병아리의 등 피부에 310nm 자외선 조사시 상이한 선양이 혈장내 비타민 $D_3$함량에 미치는 영향)

  • 장윤환;원지훈;강훈석;김강수;여영수
    • Korean Journal of Poultry Science
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    • v.19 no.1
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    • pp.47-53
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    • 1992
  • This study was carried out to determine the plasma vitamin $D_3$($D_3$) of 3 weeks old broiler chicks (Hubbard line), whose dorsal skin was exposed to different dose of 310 m UV light. The irradiated doses were 0~0.79 J/$\textrm{cm}^2$(6 doses$\times$5 replica=30 chicks). The blood samples were collected 2 days after exposure. The lipid in plasma isolated was extracted with ethyl acetate. The $D_3$ was purified by $C_{18}$ cartridge and analyzed by HPLC. The average $D_3$ content in plasma of control chicks was 20 ng /$m\ell$. When 0.32 J/$\textrm{cm}^2$ of dose was treated, the highest amount of 43 ng /$m\ell$ was found among those of chicks experimented, corresponding to 2.15 times that of control. However, When more doses than 0.32 J/$\textrm{cm}^2$ were exposed to the skin, D$_3$ contents were decreased gradually. The similar to control level of $D_3$ was observed when 0.79 J/$\textrm{cm}^2$ was applied.

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Correlation of $^{137}Cs/^{60}Co$ Activity Ratio in Radwaste with Primary Coolant (원자로 냉각재와 방사성폐기물 내 $^{137}Cs/^{60}Co$ 핵종비)

  • Jee, Kwang-Yong;Park, Yeong-Jae;Pyo, Hyung-Yeol;Ahn, Hong-Joo;Kim, Won-Ho
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.5 no.1
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    • pp.9-17
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    • 2007
  • In order to compare the correlation of radioactivity ratio between the radwaste streams and the primary coolant of PWR NPPs, A RCS sampling kit was installed to primary coolant system for the collection of the radionuclides during the normal operation of NPPs. RCS samples were collected from PWR type of domestic NPPs through 2004 to 2005, and pretreated with acid microwave digestion or leaching method to assay quantitatively of several interesting radionuclides. The radioactivity ratios of $^{137}Cs\;to\;^{60}Co$ in a filter cartridge and a resin cartridge were 2.3E-2 and 7.3E-1, respectively. At a same period of the reactor operating cycle, the radioactivity ratios of $^{137}Cs\;to\;^{60}Co$ were 6.3E-1 for a evaporator bottom, 6.7E-1 for a spent resin, and 5.6E-2 for a dry active waste, so that these radwaste streams were identified as having similar characteristics with the corresponding RCS samples.

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