• The Korean Journal of Physiology and Pharmacology
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• 제16권1호
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• pp.25-30
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• 2012

Under some pathological conditions as bile flow obstruction or liver diseases with the enterohepatic circulation being disrupted, regurgitation of bile acids into the systemic circulation occurs and the plasma level of bile acids increases. Bile acids in circulation may affect the nervous system. We examined this possibility by studying the effects of bile acids on gating of neuronal (N)-type $Ca^{2+}$ channel that is essential for neurotransmitter release at synapses of the peripheral and central nervous system. N-type $Ca^{2+}$ channel currents were recorded from bullfrog sympathetic neuron under a cell-attached mode using 100 mM $Ba^{2+}$ as a charge carrier. Cholic acid (CA, $10^{-6}M$) that is relatively hydrophilic thus less cytotoxic was included in the pipette solution. CA suppressed the open probability of N-type $Ca^{2+}$ channel, which appeared to be due to an increase in (no activity) sweeps. For example, the proportion of sweep in the presence of CA was ~40% at +40 mV as compared with ~8% in the control recorded without CA. Other single channel properties including slope conductance, single channel current amplitude, open and shut times were not significantly affected by CA being present. The results suggest that CA could modulate N-type $Ca^{2+}$ channel gating at a concentration as low as $10^{-6}M$. Bile acids have been shown to activate nonselective cation conductance and depolarize the cell membrane. Under pathological conditions with increased circulating bile acids, CA suppression of N-type $Ca^{2+}$ channel function may be beneficial against overexcitation of the synapses.

• 한국ITS학회 논문지
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• 제16권5호
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• pp.152-161
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• 2017

CBTC의 통신기술은 IEEE 802.11.x 표준을 사용하고 있으며 ISM밴드 대역중 하나인 2.4GHz를 사용하게 된다. 따라서 CBTC 철도 무선시스템으로서는 통신 지연, 통신 왜곡 및 정보 손실 등이 발생 할 수 있다. 이러한 문제를 극복하기 위해 ISM 대역중 908MHz 일정진폭을 갖는 직접 시퀀스 확산스펙트럼 송신기를 제안하였다. 성능 분석을 위해 주요파라미터인 아이 다이아그램과 scatter diagram을 고려하여 특성 분석을 하였다. 또한 국부발진 신호의 위상잡음 특성은 약 60dBc/Hz로서 기준신호와 비교 시 특성이 상당히 우수함을 알 수 있었다. 또한 2차 국부 발진 신호의 기준 주파수 억압이 50dB이하가 됨을 알 수 있었다. 제안한 변조방법에서 보조신호는 양과 음의 발생확률을 균일하게 함으로써 출력스펙트럼에서의 선스펙트럼성분에 의한 간섭을 제거하였으며 비선형 증폭기를 통과한 후의 스펙트럼의 측대파의 재생율이 현저히 줄어들었음을 볼 수 있었다.

• Archives of Pharmacal Research
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• 제26권4호
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• pp.338-343
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• 2003

The purpose of the present study was to investigate the bidirectional transport of 1-anilino-8-naphthalene sulfonate (ANS) using isolated rat hepatocytes. The initial uptake rate of ANS by isolated hepatocytes was determined. The uptake process of ANS was saturable, with a $K_m of 29.1\pm3.2 \mu M and V_{max} of 2.9\pm0.1$ mmol/min/mg protein. Subsequently, the initial efflux rate of ANS from isolated hepatocytes was determined by resuspending preloaded cells to 3.0% (w/v) BSA buffer. The efflux process for total ANS revealed a little saturability. The mean value of the efflux clearance was $2.2\pm0.1 \mu$ L/min/mg protein. The efflux rate of ANS from hepatocytes was markedly decreased at $4^{\circ}C$, indicating that the apparent efflux of ANS might not be attributed to the release of ANS bound to the cell surface, but to the efflux of ANS from intracellular space. The efflux clearance was furthermore corrected for the unbound intracellular ANS concentration on the basis of its binding parameters to cytosol. The relation between efflux rate and unbound ANS concentration was fitted well to the Michaelis-Menten equation with a saturable and a nonsaturable components. The $V_{max} and K_m$ values were 0.54 mmol/min/mg protein, and 10.0 $\mu$ M, respectively. Based on the comparison of the ratios of $V_{max} to K_m (V_{max}/K_m)$ corresponding to the transport clearance, the influx clearance was two times higher than the efflux clearance. Together with our preliminary studies that ATP suppression in hepatocytes substantially inhibited ANS influx rate, we concluded that the hepatic uptake of ANS is actively taken up into hepatocytes via the carrier mediated transport system.

• 한국정보통신학회논문지
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• 제5권5호
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• pp.869-876
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• 2001

현대 무선 통신에서는 제한된 주파수 자원을 효율적으로 사용하기 위해 스펙트럼 효율이 매우 중요한 문제로 대두되고 있다. 또한 시스템구조 측면에서는 저 가격, 저 전력 및 초경량에 목표를 두고 제작되고 있다. 본 논문에서는 직접 디지털 주파수합성기(DDS ; Direct Digital Synthesizer)를 이용하여 스펙트럼 효율이 우수한 BCPFSK(Binary Continuous Phase Frequency Shift Keying)를 직접 변조하였으며, 시스템 구조로는 직접 변환(Direct Conversion) 구조를 적용하여, 433 MHz대의 근거리 구내무선용 BCPFSK 모뎀을 설계하고 그 성능을 평가하였다. 측정결과, BCPFSK방식은 기존의 변조방식에 비해 스펙트럼 및 전력효율이 우수한 방식임을 알았다. 또한, 설계된 시스템의 측정으로 출력주파수의 중심주파수는 433.92MHz, 반송파억압비는 약 33dBc, LO 누설은 69dBc, ISM 대역외 스퓨리어스는 60dBc이하로 우수한 성능을 얻을 수 있었다.

• 전력전자학회논문지
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• 제19권6호
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• pp.488-493
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• 2014

This paper presents a carrier-based pulse-width modulation(PWM) method for reducing the common-mode voltage of a three-level four-leg converter. The idea of the proposed PWM method is intuitive and easy to be implemented in digital signal processor-based converter control systems. On the basis of the analysis of space-vector PWM(SVPWM) and sinusoidal PWM(SPWM) switching patterns, the fourth leg pole voltage of the three-phase converter called "f leg pole voltage" is manipulated to reduce the common-mode voltage. To synthesize f leg pole voltage for the suppression of the common-mode voltage, positive and negative pole voltage references of f leg are calculated. An offset voltage is also deduced to prevent the distortion of a, b, and c phase voltages. The feasibility of the proposed PWM method is verified by simulation and experimental results. The common-mode voltage of the proposed PWM method in peak-to-peak value is 33% in comparison with that of the conventional SVPWM method. The transition number of the common-mode voltage is also reduced to 25%.

• The Korean Journal of Physiology and Pharmacology
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• 제3권5호
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• pp.513-519
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• 1999

Direct exposure of renal tubular brush-border membranes (BBM) to free cadmium (Cd) causes a reduction in phosphate (Pi) transport capacity. Biochemical mechanism of this reduction was investigated in the present study. Renal proximal tubular brush-border membrane vesicles (BBMV) were isolated from rabbit kidney outer cortex by Mg precipitation method. Vesicles were exposed to $50{\sim}200\;{\mu}M\;CdCl_2$ for 30 min, then the phosphate transporter activity was determined. The range of Cd concentration employed in this study was comparable to that of the unbound Cd documented in renal cortical tissues of Cd-exposed animals at the time of onset of renal dysfunction. The rate of sodium-dependent phosphate transport $(Na^+-Pi\;cotransport)$ by BBMV was determined by $^{32}P-Iabeled$ inorganic phosphate uptake, and the number of $Na^+-Pi$ cotransporters in the BBM was assessed by Pi-protectable $^{14}C-labeled$ phosphonoformic acid $([^{14}C]PFA)$ binding. The exposure of BBMV to Cd decreased the $Na^+-Pi$ cotransport activity in proportion to the Cd concentration in the preincubation medium, but it showed no apparent effect on the Pi-protectable PFA binding. These results indicate that an interaction of renal BBM with free Cd induces a reduction in $Na^+-Pi$ cotransport activity without altering the carrier density in the membrane. This, in turn, suggest that the suppression of phosphate transport capacity $(V_{max})$ observed in Cd-treated renal BBM is due to a reduction in $Na^+-Pi$ translocation by existing carriers, possibly by Cd-induced fall in membrane fluidity.

• Journal of Microbiology and Biotechnology
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• 제16권10호
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• pp.1491-1498
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• 2006

The feasibility of pullulan acetate nanoparticles (PAN) with ionic strength (IS) sensitivity as a radioisotope carrier to inhibit tumor growth is demonstrated. PAN was radiolabeled with rhenium 188 (Re-188) without any chelating agents. The labeling efficiency of Re-188 into PAN (Re-188PAN) was $49.3{\pm}4.0%$ as determined by TLC. The tumor volumes of mice treated with 0.45 mCi of Re-188-PAN were measured and compared with that of free Re-188 after 5 days of intratumoral injection. For the histological evaluation of apoptotic nuclei of tumor cells, hematoxylin and eosin (H&E), and terminal deoxynucleotidyl transferase biotinylated deoxyuridine triphosphate nick end labeling (TUNEL) staining were performed. The mean tumor volume of the Re-188-PAN-treated group was decreased by 36% after 5 days, whereas that the free Re-188-treated group was decreased by only 15% (P<0.05). The mean number of TUNEL-positive cells in Re-188-PAN-treated tumors at $144.3{\pm}79.9$ cells/section was significantly greater than the control ($26.7{\pm}7.9$ cells/section, P=0.03). The numbers of leukocyte and lymphocyte were decreased in both free Re-188- and Re-188-PAN-treated mice. These results indicated that the intratumoral injection of Re-188-PAN effectively inhibits the tumor growth by prolonging Re-188 retention time in tumor site induced by the IS sensitivity.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2013년도 제45회 하계 정기학술대회 초록집
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• pp.75.2-75.2
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• 2013

Atomic layer deposition (ALD), utilizing self-limiting surface reactions, could offer promising perspectives for future efficient energy conversion devices. The capabilities of ALD for surface/interface modification and construction of novel architectures with sub-nanometer precision and exceptional conformality over high aspect ratio make it more valuable than any other deposition methods in nanoscale science and technology. In the context, a variety of researches on fabrication of active materials for energy conversion applications by ALD are emerging. Among those materials, one-dimensional nanotubular titanium dioxide, providing not only high specific surface area but also efficient carrier transport pathway, is a class of the most intensively explored materials for energy conversion systems, such as photovoltaic cells and photo/electrochemical devices. The monodisperse, stoichiometric, anatase, TiO2 nanotubes with smooth surface morphology and controlled wall thickness were fabricated via low-temperature template-directed ALD followed by subsequent annealing. The ALD-grown, anatase, TiO2 nanotubes in alumina template show unusual crystal growth behavior which allows to form remarkably large grains along axial direction over certain wall thickness. We also fabricated dye-sensitized solar cells (DSCs) introducing our anatase TiO2 nanotubes as photoanodes, and studied the effect of blocking layer, TiO2 thin films formed by ALD, on overall device efficiency. The photon convertsion efficiency ~7% were measured for our TiO2 nanotubebased DSCs with blocking layers, which is ~1% higher than ones without blocking layer. We also performed open circuit voltage decay measurement to estimate recombination rate in our cells, which is 3 times longer than conventional nanoparticulate photoanodes. The high efficiency of our ALD-grown, anatase, TiO2 nanotube-based DSCs may be attributed to both enhanced charge transport property of our TiO2 nanotubes photoanode and the suppression of recombination at the interface between transparent conducting electrode and iodine electrolytes by blocking layer.

• 한국항해항만학회지
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• 제30권10호
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• pp.847-853
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• 2006

본 논문에서는 부성저항 특성을 갖는 발진기 이론을 적용하여 직렬 궤환형 유전체 공진 발진기를 구성하고 바랙터 다이오드를 삽입하여 전압 제어 유전체 공진 발진기를 제작한 후, 샘플링 위상 비교기와 루프 필터를 결합한 PLL 방식을 도입하여 고안정 주파수 발생기인 위상고정 유전체 공진형 발진기를 설계 및 제작하였다. 설계 제작한 PLDRO는 주파수 12.05 GHz에서 13.54 dBm의 출력 전력을 얻었으며, 이때의 주파수 가변 동조 범위는 중심 주파수에서 약 ${\pm}7.6 MHz$ 이며, 전력 평탄도는 0.2 dBm으로서 매우 우수한 선형 특성 결과를 얻었다. 또한 데이터 전송시 오율특성에 상당한 영향을 미치는 위상 잡음은 반송파로부터 100 kHz 떨어진 offset 지점에서 -114.5 dBc/Hz을 얻었다. 고조파 억압 특성은 2 차 고조파에서 -41.49 dBc 이하의 특성을 나타내었다. 이러한 특성은 위상고정을 하기 전의 전압 제어 발진기보다 더욱 향상된 특성을 보였으며, 종전의 PLDRO보다 위상 잡음과 전력 평탄도면을 개선시킬 수가 있었다.

• 한국전자파학회논문지
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• 제18권7호
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• pp.839-846
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• 2007

본 논문에서는 새로운 구조의 이중 대역 feedforward 선형 전력 증폭기(FFW LPA)의 설계 방법을 제안하였다. 현재까지 이중 대역 FFW LPA 설계의 기술적인 어려움은 단일 대역 FFW LPA에서 사용되는 신호 상쇄기를 이중 대역 동작으로 확장시키기가 쉽지 않았다는 점이다. 따라서 본 논문에서는 이중 대역 동일 군속도 지연 주 신호 상쇄기와 혼변조 왜곡 신호(Intermodulation Distortion: IMD) 상쇄기의 설계 방법, 그리고 이를 이용하여 단일 대역 FFW 기법을 확장한 이중 대역 FFW LPA의 설계 방법을 제안하였다. 제안하는 설계 방식의 효용성을 검증하기 위하여, 동작 대역의 중심 주파수를 기준으로 1.26 GHz 이격된 디지털 셀룰러 대역($f_0=880$ MHz)과 IMT-2000($f_0=2.14$ GHz) 대역 기지국용 이중 대역 feedforward 선형화 시스템을 구현하였다. 각 대역별로 CDMA IS-95A 4FA 신호와 WCDMA 4FA 신호를 인가하여 인접 채널 누설비(Adjacent Channel Leakage Ratio: ACLR)의 개선 정도를 측정한 결과, 디지털 셀룰러 대역에서 평균 출력 전력 41.5 dBm일 때 16.52 dB, IMT-2000 대역에서 평균 출력 전력 40 dBm일 때 18.59 dB의 개선 효과를 동시에 얻을 수 있었다.