• 대한한의학원전학회지
• /
• 제29권1호
• /
• pp.99-111
• /
• 2016

Objectives : The researcher figured out the kinds of fish that were used as a treatment for various diseases, and based on the result, studied on how to enhance healthy life and facilitate disease treatment with fish-based treatment. Methods : The researcher first extracted prescriptions based on fish from Singnyochanyo and later, analyzed the extracts by main efficacies, ingredients, recipes and intake periods. Results & Conclusions : According to study, Singnyochanyo used snakeheads twice, eels eleven times, crucian carps eight times, gray mullets once, carps twelve times and yellow croakers three times. When it comes to crucian carps, Singnyochanyo utilized them for asthma-related symptoms, stomach-related diseases, diarrhea, dysentery and hemorrhoid. Donguibogam, however, used crucian carps for more specific purposes - dysentery and stomach-related diseases. In Singnyochanyo, gray mullets and yellow croakers were used to treat stomach-related diseases only one to three times. Carps were applied to treat diseases twelve times in total, five of them for pregnancy-related diseases. For pregnant women, it was recommended to make a soup of carps with side ingredients.

• Asian-Australasian Journal of Animal Sciences
• /
• 제17권9호
• /
• pp.1197-1203
• /
• 2004

This study deals with the characterization of three populations (two hatchery and one natural) of Indian major carps Catla catla and Labeo rohita from different locations in India. The genetics of Indian major carps has been completely obscure and this is the first report on comparative allozyme variations in natural and hatchery population. The total 10 biochemical genetic markers used to measure interspecific and intraspecific level of diversity. The allele frequency data indicate different level of genetic variability in three populations. The hatchery population exhibited least polymorphism, low level of heterozygosity and genetic diversity.

• Asian-Australasian Journal of Animal Sciences
• /
• 제15권5호
• /
• pp.740-744
• /
• 2002

The relationship of gonad weight and liver weight with body weight of six fish species viz; Catla catla, Labeo rohita, Cirrhina mrigala, Hypophthlamichthys molitrix, Ctenopharyngodon idella and Cyprinus carpio under the influence of artificial feed, broiler manure, buffalo manure, N:P:K (25:25:0) and a control pond were examined after a rearing period of one year. The positive relationship between gonad weight and body weight was significant which showed the dependence of gonadal development on body weight in all the six fish species. The currelation coefficients were higher in female fishes. However, the major carps had a much smaller proportional gonadal weight as compared with Chinese carps and a common carp due to their faster growth rate. The overall comparison of six fish species under different experimental treatments revealed that highest liver weight was observed for C. idella closely followed by C. carpio. The maximum correlation value was observed with H. molitrix under the broiler manure. The maximum Gonadosomatic Index (GSI) remained as 32.63 for C. carpio followed by C. idella. The maximum value for Hepatosomatic Index (HSI). remained 1.99 for C. idella followed by C. mrigala.

• BMB Reports
• /
• 제39권4호
• /
• pp.346-354
• /
• 2006

The full-length cDNA of grass carp (Ctenopharyngodon idellus) and silver carp (Hypophthalmichthys molitrix) uncoupling protein 2 (UCP2) was obtained from liver. The grass carp UCP2 cDNA was determined to be 1152 bp in length with an open reading frame that encodes 310 amino acids. Five introns (Intron 3, 4, 5, 6 and 7) in the translated region, and partial sequence of Intron 2 in the untranslated region of grass carp UCP2 gene were also obtained. Gene structure comparison between grass carp and mammalian (human and mouse) UCP2 gene shows that, the UCP2 gene structure of grass carp is much similar to that of human and mouse. Partial UCP2 cDNA sequences of bighead carp (Aristichthys nobilis) and mud carp (Cirrhinus molitorella), were further determined. Together with the common carp (Cyprinus carpio) UCP2 sequence from GenBank (AJ243486), multiple alignment result shows that the nucleotide and amino acid sequences of the UCP2 gene, were highly conserved among the five major Chinese carps that belong to four subfamilies. Using beta-actin as control, the ratio UCP2/beta-actin mRNA (%) was determined to be $149.4{\pm}15.6$ (common carp), $127.4{\pm}22.1$ (mud carp), $96.7{\pm}12.7$ (silver carp), $94.1{\pm}26.8$ (bighead carp) and $63.7{\pm}16.2$ (grass carp). The relative liver UCP2 expression of the five major Chinese carps, shows a close relationship with their food habit: benthos and detrituseating fish (common carp and mud carp) > planktivorious fish (silver carp and bighead carp) > herbivorious fish (grass carp). We suggest that liver UCP2 might be important for Chinese carps to detoxify cyanotoxins and bacteria in debris and plankton food.

• 한국환경농학회지
• /
• 제3권1호
• /
• pp.36-44
• /
• 1984

낙동강(洛東江) 류역중(流域中) 8개(個) 지점(地點)에서 1982년(年) 8월(月)부터 1983년(年) 6월(月)까지 2개월(個月) 간격으로 채취(菜取)한 수질(水質) 48점(點), 저이토(底泥土) 48점(點) 및 붕어 29점(點)에 대하여 유기린계(有機燐系) 기류농약(幾留農藥)을 FPD부착(附着) GLC로 분석(分析)한 결과(結果)를 요약(要約)하면 다음과 같다. 1) 수질(水質), 저이토(底泥土) 석시(析時) 원소황(元素黃)의 간사(干沙)은 copper powder 또는 raney nickel로 제거(除去)하였다. 2) 낙동강(洛東江) 환경시료(環境試料)중 수질(水質)과 붕어에서 유기린계(有機燐系) 잔유농약(殘留農藥)이 검출(檢出)되었으나 저니토(底泥土)에서는 검출(檢出)되지 않았다. 3) 농약(農藥) 성수기(盛需期)인 8월(月)에 채취(採取)한 수질(水質)과 붕어시료(試料)에서는 IBP, diazinon, phenthoate, parathion, malathion 및 fenitrothion 등(等) 6종(種)의 유기린계(有機燐系) 농약(農藥)이 검출(檢出)되었다. 4) 수질(水質) 및 붕어중(中) 유기린계(有機燐系) 농약(農藥)의 잔유량(殘留量)은 계절적(季節的) 변이(變異)가 인정되었으며, 수질(水質) 및 붕어중(中) diazinon과 IBP의 천유량(淺留量)이 가장 많았고, phenthoate, parathion, malathion 및 fenitrothion은 검출빈도(檢出頻度) 및 잔유수준(殘留水準)이 낮았다.

• Animal cells and systems
• /
• 제5권4호
• /
• pp.333-339
• /
• 2001

Common carp (Cyprinus carpio) and its aquaculture breed Israeli carp samples were obtained from two separate aquaculture facilities under the similar raising conditions during two years in the Kunsan National University, Korea. Genomic DNA was isolated from the common carp and Israeli carp for identification of genetic characteristics and genomic polymorphisms by polymerase chain reaction amplification of DNA using arbitrary primers. The arbitrary primer No.21 (ACTTCGCCAC) yielded the highest number of fragments with the average of 15.0 among the primers used in Israeli carp. A tota1 of 294 polymorphic products in common carp and 336 in Israeli carp were observed by random primers. The average number of polymorphic products generated by random RAPD primer No. 2 (GTAGAC-CCGT) showed 8.0 in Israeli carp. On average, each random RAPD primer produced 5.4 amplified polymorphic products in common carp and 6.2 in Israeli carp. An average genetic similarity (BS value) was 0.44$\pm$0.05 within the common carp and 0.32$\pm$0.04 within the Israeli carp. The degree of similarity frequency (BS) between two carps was 0.67 as generated by the primer No. 19 (GACGGATCAG). The average level of bandsharing was 0.57$\pm$0.03 between the two carps. Accordingly, the two carp populations were genetically a little distant. The electrophoretic analysis of PCR-RAPD products showed middle levels of variation between the two carp populations. This result implies that the genetic diversity among intra-population may be higher when compared with that between the two carps. The RAPD polymorphism generated by these random primers might be used as a genetic marker for populations or lines identification in important aquacultural carp.

• 생태와환경
• /
• 제34권2호통권94호
• /
• pp.98-105
• /
• 2001

2개월된 어린 백연어의 성장에 있어서 7종의 배양조류와 동물플랑크톤의 먹이 기여도를 조사하기 위하여 10L의 원통형 수조에 8일동안 각각 배양하였다. 동물플랑크톤 Moina는 대부분의 배양조류를 효과적으로 섭취하였는데, Cryptomonas의 경우, 60분동안에 거의 대부분의 조류를 제거한 반면 남조류 Oscillatoria와 Microcystis는 강하게 Moina개체군을 감소시켰다. 어린 백련어는 52시간동안에 배양조류의 약 50% 정도 섭취하였으나, 남조 Microcystis의 경우 60% 이상이 여전히 수조에 남았으며, 동시에 어류체중의 5${\sim}$12%를 감소시켰다. 실험에 사용된 동일한 밀도로 계속적으로 투입할 경우, 어린 백련어는 11${\sim}$65일만에 사멸하였다. 동물플랑크톤 Moina는 투입된 어류밀도에 따라 각 개체의 성장특성이 다소 차이가 있으나 단일배양조류보다 어류성장을 유도하는 가장 직접적인 영양원으로 사료되었다.

• 농약과학회지
• /
• 제10권4호
• /
• pp.289-295
• /
• 2006

살충제 imidacloprid(1-(6-chloro-3-pyridylmethyl)-N-nitroimidazolidin-2-ylideneamine)의 붕어(Carassius auratus L.) 체내 행적과 장기에 미치는 영향을 구명하기 위하여 imidacloprid를 환경추정농도인 0.064 mg $kg^{-1}$로 처리한 시험수에 붕어를 4일간 경시적으로 노출시켰다. 수중의 imidacloprid는 붕어체내에 흡수되어 노출 2일차에 최대 흡수량을 나타내었으며, 다른 장기에 비해 쓸개에서 농도가 가장 높았다. 붕어 사육수에서 미확인 주대사산물과 imidacloprid urea가 대사산물로 검출되었다. 또한 붕어체내에 흡수된 imidacloprid의 주요 배설경로는 담도이었으며, 장기에 대한 조직학적 이상증상은 발견되지 않았다.

• 한국환경독성학회:학술대회논문집
• /
• 한국환경독성학회 2002년도 춘계학술대회(Genomics, Proteomics, and Risk Assesment):환경독성 및 위해성 평가 국제 심포지움
• /
• pp.68-68
• /
• 2002

• Environmental Analysis Health and Toxicology
• /
• 제12권3_4호
• /
• pp.55-59
• /
• 1997

Furfural, an organic solvent, is widely used as synthetic component material in producing chemical products. However, furfural has been reported that it shows strong toxicities to human being showing intense stimulus to skin, eyes, mucous membrane and nerve system. It is also known to cause anemia, liver cirrhosis, kidney failure and genetic toxicity in the human being working in the exposed area. LD$_{50}$ of furfural for peritoneal injected mouse has been known around 20mg/kg, but the acute toxicity on aquatic organisms such as fish, daphnid or algae are not well known, compared to those on rodents. In this experiment, we studied on the fish toxicity of furfural using Japanese Medaka (Orvzias latipes) and Common Carp (Cvprinus carpio). We also observed histological changes in the fish organs. The LC$_{50}$ were 12. Smg/L in Japanese Medaka and 21.8 mg/L in Common Carp, respectively. When Common Carps were exposed to 120mg/L of furfural concentration for 30 minutes, blood congestion in gills and lysis of secondary lamella were shown. Though the muscle of caudal fin was not completely eroded, its epidermic cells were shown to be necrotic in various parts. Tissue atrophy and cell necrosis were also shown in the liver of Common Carps exposed to furfural. From these results, furfural seems to cause histological damages on liver, an internal organ as well as on external organs such as gills and fins eventhough the fish were exposed for a short-term.