• 대한소아치과학회지
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• 제47권4호
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• pp.406-415
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• 2020

Silver diamine fluoride(SDF)는 우식의 정지를 위해 사용될 수 있는 효과적이고 경제적인 약제로, 협조가 힘든 환자나 의과적인 문제로 치료를 받기 힘든 경우 등에서 사용될 수 있다. 이 연구는 타액 생물막을 이용하여 SDF 의 항미생물효과를 평가하고자 시행되었다. 타액을 이용하여 획득피막 유사 구조를 형성한 후, 타액과 Streptococcus mutans를 첨가하여 우식원성의 타액 생물막을 형성하였다. 타액 생물막에 SDF 를 처리한 결과, 전체 미생물과 S. mutans의 수가 유의하게 감소하였다(p < 0.000). CLSM에서도 생물막 내 죽은 세포가 증가하였다. 유치 법랑질과 상아질 시편에 SDF를 처리한 후 우식원성의 생물막을 배양한 결과, SDF 를 처리할 경우 생물막 형성이 저해되는 것을 확인할 수 있었다(p = 0.029). 연구 결과 SDF의 뛰어난 항미생물효과를 확인할 수 있었으며, 이로 인한 임상에서의 항우식효과를 기대할 수 있다.

• 미생물학회지
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• 제48권1호
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• pp.52-56
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• 2012

본 연구는 치아우식증 및 치주질환 원인균들에 대한 carvacrol의 항균능을 알아보고, 사람의 구강조직 세포에서 세포의 생존율을 분석하여 세포독성 정도를 알아보기 위해 실시하였다. Carvacrol의 항균능은 뮤탄스 연쇄상구균과 5종류의 치주질환 원인균을 이용하여 MIC 및 MBC 값을 측정하여 분석하였고, 세포생존율은 정상 사람 치은섬유모세포를 이용하여 MTT 분석법으로 평가하였다. 구강질환 원인균에 대한 carvacrol의 MIC 및 MBC 값은 각각 $16-128{\mu}g/ml$과 $32-128{\mu}g/ml$이었다. 세포독성 실험 결과 carvacrol은 $128{\mu}g/ml$ 농도에서 세포생존율이 현저히 감소하였다. 따라서 carvacrol은 치아우식증과 치주질환 원인균에 대한 항균작용이 뛰어나지만, 구강조직 세포에 대해 세포독성을 가지고 있어 가글린제 및 치약 등의 구강위생용품에 활용하기 위해서는 $64{\mu}g/ml$ 이하의 농도로 사용하고 정상 사람 치은섬유모세포에 대한 독성이 없는 다른 항균물질과 혼합하여 사용하는 것이 효과적일 것으로 생각된다.

• 한국치위생학회지
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• 제9권2호
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• pp.13-24
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• 2009

The purpose of this study was to examine the relationship between the dietary habits and oral health of elderly people in an attempt to pave the way for the development of oral health promotion programs geared toward improving the quality of life of the elderly. The subjects in this study were senior citizens who were selected by convenience sampling from Seoul. The findings of the study were as follows: 1. Regarding subjective oral health state, 54.5% of the elderly people, more than the half, considered their mouth to be in good health. The number of their mean remaining permanent teeth was 13.71. 47.5 percent of the senior citizens investigated had no shaking teeth. 2. Those who found themselves to be in good oral health had meals on a regular basis(p=0.022) and ate detergent food often. The gaps between them and the others was significant(p=0.005). In contrast, the elderly people who were in a bad oral health frequently ate cariogenic food(p=0.044). 3. The elderly people who had 21 teeth or more ate detergent food often(p=0.029), and those who owned no teeth had a sweet teeth(p=0.003), ate more cariogenic food(p=0.001) and had a snack frequently(p=0.026). 4. The subjective oral health status had a positive correlation to detergent food intake(r=0.23) and had a slightly negative correlation to preference for sweets(r=-0.14), cariogenic food intake(r=-0.14) and snack intake(r=-0.06). The number of tooth was positively correlated to detergent food intake(r=0.23) and negatively to preference for sweets(r=0.32), cariogenic food intake(r=-0.30) and snack intake(r=-0.21). The presence or absence of shaking teeth had a positive correlation to snack intake(r=0.14). The above-mentioned findings suggested that the dietary habits of the elderly people had a statistically significant relationship to subjective and objective oral health state, which indicated that there was a close relationship between oral health and dietary habits. Therefore how to improve dietary habits as well as oral health should be taken into account when oral health promotion programs are developed for the sake of the elderly. That would contribute to promoting the oral health of elderly people and eventually boosting their quality of life.

• 대한소아치과학회지
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• 제49권2호
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• pp.140-148
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• 2022

이 연구는 우식원성 세균막 형성과 상아질 표면 거칠기에 대한 Silver diamine fluoride와 요오드화 칼륨(KI)의 영향을 실험실 환경에서 평가하는 것이다. 인공 우식을 유발한 48개의 Bovine dentin 시편을 제작하여 대조군, SDF 도포, SDF와 KI 도포(SDFKI)의 3개 군으로 나누었다. 각 군당 10개의 시편의 상면에 Streptococcus mutans, Lactobacillus casei 및 Candida albicans를 포함하는 다종 우식원성 세균을 24시간 동안 배양 후 세균 수를 측정하였다. 나머지 시편은 원자 힘 현미경과 주사전자현미경으로 관찰하였다. SDF와 SDFKI 도포 시 대조군에 비해 세균 수가 유의하게 감소했으며, KI는 SDF의 항균 효과를 유의하게 저해하지 않았다. 현미경 관찰 시 SDF와 SDFKI 도포 후 생성되는 입자가 상아질 표면에 침착 되는 것을 관찰했으나 표면 거칠기는 세 군 간 유의한 차이가 없었다.

• 약학회지
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• 제34권2호
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• pp.106-111
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• 1990

The isolation and identification of an antibacterial component, from the leaves of Liriodendron tulipifera. K. Kotch against a cariogenic bacterium Streptococcus mutans OMZ 176, were carried out for developing of anticariogenic agents. The bioactive component was elucidated as ${\beta}-liriodenolide$, which was isolated newly from the leaves of L. tulipifera. The minimal inhibitory concentration (MIC) of ${\beta}-liriodenolide$ was $100\;{\mu}g/ml$ and the antibacterial activity was stronger than that of berberine. ${\beta}-Liriodenolide$ inhibited ${\beta}-lactamase$ activity, 50, 100 and $200\;{\mu}M$ ${\beta}-liriodenolide$ did ${\beta}-lactamase$ activity as 0.7, 3.5 and 19.7%, respectively. The toxicity of ${\beta}-liriodenolide$ was not found with the method of photohemolysis.

• 약학회지
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• 제34권4호
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• pp.277-281
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• 1990

The isolation and identification of an antibacterial component, from Polygoni Radix against a cariogenic bacterium Streptococcus mutans OMZ 176, were carried out for development of anticariogenic agents. The bioactive component was identified to be emodin. The minimal inhibitory concentration (MIC) of emodin was $100\;{\mu}g/ml$ against S. mutans OMZ 176. The bioactive component emodin weakly inhibited ${\beta}-lactamase$ activity with the inhibition ratio of 1.7, 4.3 and 7.6% at the concentration of 50, 100, and 200 uM, respectively. Emodin exhibited slight phototoxicity when analysed by the photohemolysis method.

• 대한치과의사협회지
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• 제15권2호
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• pp.145-151
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• 1977

The author performed Snyder test, estimation of salivary flow rate, salivary viscosity test, salivary buffering capacity test, M-R test, oral hygiene ability test, estimation of oral glucose clearance time and plaque reformation test in the 107 male Koreans aged form the age of 20 to 29, in order to detect and control the active cariogenic factors contributing to caries development on the individual basis. Thereafter, the data from 8 kinds of caries activity test were analysed and evaluated. The obtained results were as follows: 1. Snyder test was positive in 86.0% of total tested persons, and among those showing positive Snyder test reaction, slight caries activity was appeared in 39.1%, moderate caries activity in 23.9%, and marked caries activity in 37.0%. 2. Salivary flow rate was under the average flow amount in 53.3%. 3. Salivary buffering capacity was low in 22.4%. 5. Buffering capacity of dental plaque was high in 12.5%. 6. Oral hygiene ability was insufficient in all persons tested. 7. Oral glucose clearance time was long in 42.1%. 8. Plaque reformation rate was high in 8.4% of the 107 persons tested.

• 한국축산식품학회지
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• 제42권6호
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• pp.1020-1030
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• 2022

The aim of the study was to investigate the effect of bacteriocin-like inhibitory substance (BLIS) from Enterococcus faecium DB1 on cariogenic Streptococcus mutans biofilm. Crystal violet staining, fluorescence, and scanning electron microscopy analyses demonstrated that the BLIS from Enterococcus faecium DB1 (DB1 BLIS) inhibited S. mutans biofilm. When DB1 BLIS was co-incubated with S. mutans, biofilm formation by S. mutans was significantly reduced (p<0.05). DB1 BLIS also destroyed the preformed biofilm of S. mutans. In addition, DB1 BLIS decreased the viability of S. mutans biofilm cells during the development of biofilm formation and in the preformed biofilm. DB1 BLIS significantly decreased the growth of S. mutans planktonic cells. Furthermore, S. mutans biofilm on the surface of saliva-coated hydroxyapatite discs was reduced by DB1 BLIS. Taken together, DB1 BLIS might be useful as a preventive and therapeutic agent against dental caries caused by S. mutans.

• 대한소아치과학회지
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• 제35권1호
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• pp.39-46
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• 2008

유아기우식증의 식이요인에 관한 연구의 일환으로, 국내외에서 시판되고 있는 4종의 유아용 과자의 우식유발능을 완충능, 산생성능, 탈회능 등의 요인으로 구분하여 생체외 실험을 통해 연구하였다. 국내에서 시판되는 4종의 유아용 과자를 실험군으로 하고, 10% 자당용액과 우유를 대조군으로 하였다. 시료용액 50ml의 pH를 4.0까지 낮추는 데 소모된 0.1N 유산용액의 양으로 완충능을 측정하였고, Streptococcus mutans ATCC 25175의 배양액을 접종하여 48시간 배양하기 전과 후에 각각 시료용액의 pH, 시료용액 속에 담근 유치 법랑질 시편의 표면미세경도를 측정하여 산 생성능과 탈회능을 평가하여 다음과 같은 결과를 얻었다. 유아용 과자의 완충능은 자당용액보다 크고 우유보다 작았으며 유아용 과자간에 유의한 차이가 있었다(p<0.05). 48시간 배양 후 유아용 과자의 평균 pH는 $3.93{\pm}0.26$으로서 자당용액의 $3.93{\pm}0.12$와 유사하였고 우유와는 유의한 차이가 있었으며(p<0.05), 유아용 과자 상호간에도 유의한 차이가 있었다(p<0.05). 배양 전후의 유치 법랑질 표면미세경도의 감소율 차이는 유아용 과자군이 평균 23.02%로 자당용액군의 25.09%와 비슷하였고 우유군의 10.34%보다 유의하게 컸다(p<0.05). 국내외 시판 유아용 과자의 우식유발능은 생체외 연구에서 유치의 치아우식증을 유발할 수 있는 기본 조건을 갖추고 있는 것으로 나타났으며, 유아용 과자 상호간에 부분적으로 유의한 차이가 있었다. 유아기우식증의 복합적 발생요인 중에서 식이조절을 조절하기 위하여, 유아용 과자의 우식유발능을 더욱 낮추기 위한 학계와 산업계의 공동 노력이 필요하다고 사료되었다.

• 한국학교보건학회지
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• 제14권1호
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• pp.73-81
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• 2001

The purpose of this study was to collect basic data for the development of effective oral health education program in elementary school. The subjects in this study Were 604 fourth-year, fifth-year and sixth-year students of an elementary school in Seoul, Oral health awareness and behaviors were surveyed and analyzed. The findings of this study were as follows: 1. The most common time for toothbrushing was after dinner(62.0%) and before bedtime(62.3%). By school year, the largest number who brushed their teeth after dinner were fourth and fifth graders while the greatest number who did so after breakfast were sixth graders. Seventy-four percent of respondents brushed the teeth twice or more a day. By school year, the fifth-year students brushed their teeth the least while girls outperformed the boys in daily toothbrushing frequency(p<0.05). Regarding the intake of cariogenic food, 62.1% took that kind of food once or more a day. By school year, the fifth graders took the most (p<0.05), and girls took more cariogenic food than the boys(p<0.05). 2. Ninety percent of the respondents had visited a dental clinic. By grade, the sixth-year students had visited a dental clinic more frequently and girls more frequently than boys. Eighty percent of the respondents visited a dental clinic to receive treatment but just 12.3% visited a dental clinic to prevent oral diseases. Concerning the fear of dental treatment, only 14.4% expressed fear. School year made no difference, yet the percentage of girls who experienced fear was higher than that of boys(p<0.05). Regarding preventive treatment, 39.7% experienced pit and fissure sealing and 24.2% experienced the application of fluorides. All the students hadn't much experience in preventive treatment regardless of school year -- gender didn't make a difference. 3. Fourty-six percent of respondents had a preference for the fluoride mouth rinsing program; 38.4% wanted that program to continue. As to the reason for preference, 38.2% preferred it because of the prevention of dental caries, 43.0%, the largest percentage, didn't favor it because they found it too much trouble to do(some of the respondents gave two different answers). 4. Despite an increase in the frequency of toothbrushing at the right time, they still took cariogenic food frequently. Their visit to a dental clinics also aimed at treatment more than at prevention, and they had very narrow experience of preventive treatment Furthermore, the fluoride mouth rinsing program failed to draw their interest Therefore, it is recommended that a successful oral health education program should be developed.