• International Journal of Oral Biology
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• 제39권4호
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• pp.201-206
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• 2014

This study investigated the antimicrobial activity of methanol extract of mulberry leaf against 16 strains of mutans streptococci and four species of periodontopathogens: Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans. The antimicrobial activities of the crude extracts or silica gel chromatography fractions of methanol-extracted mulberry leaf were evaluated by determining minimal inhibitory concentrations using an established microdilution method. The cytotoxicity of the extracts of mulberry leaf on KB cells was tested by the methyl thiazolyl tetrazolium assay. Chromatography fraction 12 displayed the most potent antimicrobial activity against all 16 strains of mutans streptococci, P. gingivalis, and P. intermedia. No KB cell cytotoxicity was evident up to $128{\mu}g/ml$ of fraction 12. The methanol extract had no antimicrobial activity against F. nucleatum and A. actinomycetemcomitans. These results suggest chromatography fraction 12 methanol extract of mulberry leaf could be useful in the development of oral hygiene products, such as dentifrice and oral hygiene solution, for the prevention of dental caries.

• 한국치위생학회지
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• 제11권5호
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• pp.801-809
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• 2011

Objectives : The Purpose of this study is to apply an oral health education program to the high school students, to analyze their oral health knowledge and changes of behaviors, and to examine oral health education for effective, thus using all of those results as the basic data for developing materials on their oral health education. Methods : The study was conducted on the freshmen and women of M high student in Seoul City. They were in total 85 student, consisting of 77 of male student(90.6%) and 8 of female student(9.4%). Knowledge survey contained 38 questions including such as dental common knowledge, dental caries, and periodontal disease, while behaviors survey did 24 questions including such as tooth-brushing, brush selection and management, and prevention of oral disease. Results : First, oral health education had brought to improve oral health knowledge for high school students. Second, even with the improvement of oral health behaviors through the education, there was not statistically significant on behaviors such as the regular checkups and the usage of dental floss. And third, the students in general were satisfied with the oral health education. Conclusions : First, the oral health education being conducted in kindergarten and elementary school should be continued or expanded into the adolescence. Second, the oral health education should be focused efficiently on the learning objective demanding for a change of behavior through the repeated education, for which the education that is right for the high school students should be done. And third, for the effective oral health education in high school, the media that could cause interests should be developed.

• 한국치위생학회지
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• 제17권6호
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• pp.1081-1094
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• 2017

Objectives: The purpose of this study is to find out how to provide the correct information about toothbrushing and to promote oral health. Methods:A survey was conducted from December 2016 with 354 subjects who are over 20 years old in South Korea to find out the influence of the using toothbrush. The results are as follows. Statistical analysis of the collected data was performed using the SPSS WIN 21.0 statistical program. Results: After reviewing the result that the using toothbrush of oral health according to the general nature, the average score of the female $3.94{\pm}0.87$ was higher than the male, the highest average score of 50-59 age was $4.10{\pm}0.83$. As for the highest average score regarding martial status, the married group's score was $3.91{\pm}0.88$. After reviewing the factors affecting the toothbrushing, the age group of 50-59 has the highest using toothbrush and the more using toothbrush in oral health, the more using toothbrush. Conclusions: Systematic dental health education program is needed to increase the correct knowledge of time to replace the toothbrush among the public. Also it is needed to increase people's toothbrushing constantly by acquiring the information through SNS, AD, and mass media.

• 한국치위생학회지
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• 제14권2호
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• pp.163-171
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• 2014

Objectives : This study aims to improve health and the quality of life of teenagers by identifying an association between the periodontal status and oral health behaviors of Korean teenagers using 2010 Korean National Oral Health Survey data. Methods : Subjects were teenagers aged from 12 to 15 in Korean National Oral Health Survey (KNOHS). The questionnaire consisted of periodontal status, general characteristics, periodontal related factors, and oral health related behaviors. Results : Gingival bleeding was found in 56.9% of teenagers because unhealthy periodontal status and tartars were found. Those who were 15 years old had unhealthier periodontal status than those who aged 12 years old. Those who lived in rural areas tended to have worse periodontal status than those who lived in the big cities. Access to dental floss, mouth rinsing solution and regular dental checkup tended to make the healthier periodontal status. Smokers had the worse periodontal status than the nonsmokers. Conclusions : The oral health care in the teenagers aged from 12 to 15 is very important because the permanent dentition is completed in this stage. It is necessary to lay emphasis on the oral health improvement and dental caries prevention program to the teenagers.

• Restorative Dentistry and Endodontics
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• 제18권2호
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• pp.341-356
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• 1993

In the prevention of root surface caries, antimicrobial therapy for the control of subgingival and supragingival plaque is seriously considered as a long term suppression of pathogenic microflora. Recently, varnishes containing antimicrobial agents have been developed to control the supragingival microflora. The purpose of this study was to determine the antimicrobial effects of 20% chlorhexidine varnish and 2.6% silane fluoride varnish with sealant. In clinical experiments, 12 subjects were selected from the periodontally treated patient and divided into 3 groups. After a dental prophylaxis, the subjects were treated with single application of placebo varnish (group I), 20% chlorhexidine varnish (group II), and 2.6% silane fluoride varnish (group III). Root surface plaque samples were taken before (baseline) and one, two, four, and 8 weeks after the treatments. Microbiological examinations of root surface plaque were performed with culture study and indirect immunoflorescence (I.I.F.) study, and immunological examination of gingival crevicular fluid antibody titers was performed with ELISA study. The results were as follows: 1. Pathogenic microflora on the root surface including S. mutans, S. sanguis, S. mitis, A. naeslundii, A. viscosus were 24 - 37% on I.I.F. study. 2. S. mutans, S. sanguis, S. mitis, A. naeslundii, A. viscosus of the root surface plaque was significantly reduced from 1 week to 8 weeks after antimicrobial varnish treatment, but showed generally increasing tendency in control group. 3. Gingival crevicular fluid antibody titers were significantly reduced from 1 or 2 weeks to 4 weeks after antimicrobial varnish treatment.

• 한국치위생학회지
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• 제16권1호
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• pp.129-136
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• 2016

Objectives: The purpose of the study is to investigate the relationship between oral health status and body mass index (BMI) in adults over 65 years old. Methods: The study subjects were 4,550 adults over 65 years old from the 5th Korea National Health and Nutrition Examination Survey(KNHANES V) in 2010-2012. Mastication-related oral health status included the number of remaining teeth, and mean number of decayed, missing, and filled permanent teeth(DMFT). Body mass index(BMI, $kg/m^2$) was categorized as underweight(<18.5), normal weight (18.5-22.9), overweight(23.0-24.9), and obese(${\geq}25.0$). Multinomial logistic regression analysis was performed to examine the association of BMI categories with the number of remaining teeth and DMFT. Results: The mean number of DMFT was highest($13.0{\pm}0.7$) in the underweight group and lowest($8.8{\pm}0.3$) in the obese group. Those having less favorable masticatory ability, and fewer number of remaining teeth and no prosthesis, tended to be underweight. Those having a higher number of remaining teeth and prosthetic teeth tended to be overweight or obese. In the multinomial logistic regression analysis, compared with those having 20 or more remaining teeth, including prosthetic teeth, those having less than 20 remaining teeth and no prosthesis had 4.48 times higher odds ratio of being underweight. DMFT was positively associated with underweight, while negatively associated with overweight or obesity. Conclusions: The masticatory ability and dental caries prevention maintained the healthy body weight in adults of old age.

• 한국치위생학회지
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• 제13권3호
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• pp.501-507
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• 2013

Objectives : The purpose of this study was to evaluate the effect of the family dentist system on oral health status of children and adolescents of Community Children's Centers in Busan, Korea. Methods : The subjects of this study were 81 children and adolescents, 6 to 14 years old, using Community Children's Centers. The oral health survey was conducted on the subjects from the starting stage of family dentist program in 2009 to the evaluation stage in 2010. Dental health status was examined by a trained dentist according to the guideline proposed by the World Health Organization. In addition, the information on the oral health knowledge, belief, and process of dental care were obtained using questionnaires. Data were analyzed using the paired samples t-test. Results : Percentages of subjects with fissure sealants on permanent teeth and filling rate among DMF teeth of 2010 year were higher than those of 2009 year (P<0.05). However, the rate of decayed teeth among DMF teeth of 2010 year was lower than those of 2009 year (p=0.049). Conclusions : These findings showed that the family dentist system brought a positive effect on caries prevention and proper dental care of children and adolescents of the Community Children's Centers.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제32권2호
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• pp.168-173
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• 2006

The side effects of head and neck radiation therapy include mucositis, xerostomia, loss of taste, radiation caries, oral infection, osteoradionecrosis and trismus. When a patient is arranged to begin head & neck radiotherapy, oral pathologic lesions are examined and managed for the prevention of oral complications. The advanced odontogenic infection should be especially controlled before the radiotherapy and the patient must be instructed for proper oral prophylaxis. Generally the more conservative treatments, such as, scaling, restoration, endodontic treatment, are the care of choice and dental extraction is performed in advanced periapical and periodontal pathologic conditions. If the dental extraction should be done, the radiotherapy consequently will be delayed until there is epithelium covering the extraction socket, leaving no exposed bone. The cancer patient with severe emotional stress pray for the early radiation therapy, in spite of possibility of the recurrent odontogenic infectious lesions. So, the authors attempted to do the early radiation therapy by the conservative endodontic drainage and surgical incision & drainage without extraction of the infected teeth, and resulted in relatively good prognosis without the severe side effects of head and neck radiotherapy.

• International Journal of Oral Biology
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• 제35권4호
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• pp.191-195
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• 2010

Oleanolic acid is a natural triterpenoid that exists widely in foods and some medicinal herbs. The purpose of this study was to determine the antimicrobial activity of oleanolic acid against Streptococcus mutans strains isolated from a Korean population. Antimicrobial activity against these bacteria was evaluated by minimal inhibitory concentration (MIC) and time kill curves. The tolerance of human gingival fibroblasts and human periodontal ligaments to oleanolic acid was tested using a methyl thiazolyl tetrazolium (MTT) assay. The $MIC_{90}$ value of oleanolic acid for both S. mutans and S. sobrinus isolated from Koreans was 8 ${\mu}g/ml$. Oleanolic acid showed bactericidal effects against S. mutans ATCC $25175^T$ and S. sobrinus ATCC $33478^T$ at $1\;{\times}\;MIC$ ($8{\mu}g/ml$) and had no cytotoxic effects against KB cells at this dose. The results suggest that oleanolic acid could be useful in the future development of oral hygiene products for the prevention of dental caries.

• International Journal of Oral Biology
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• 제43권3호
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• pp.141-146
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• 2018

Periodontitis is generally a chronic disorder characterized by breakdown of tooth-supporting tissues, producing dentition loss. Porphyromonas gingivalis (P. gingivalis), a Gramnegative anaerobic rod, is one of the major pathogens associated with periodontitis. Neutrophils are first line defense cells in the oral cavity that play a significant role in inflammatory response. Xylitol is a known anti-caries agent and has anti-inflammatory effects. In this study, we conducted experiments to evaluate anti-inflammatory effects of xylitol on P. gingivalis infected neutrophils for possible usage in prevention and treatment of periodontal infections. P. gingivalis was intraperitoneally injected and peritoneal lavage was collected for cytokine determination. For in vitro study, neutrophils were collected from mouse peritoneal cells after zymosan injection or bone marrow cells. Neutrophils were stimulated with live P. gingivalis and ELISA was used to determine the effect of xylitol on P. gingivalis induced cytokine production. $IL-1{\beta}$, IL-6, $TNF-{\alpha}$ concentration and neutrophil population in the peritoneal lavage was increased in P. gingivalis-infected mouse. Peritoneal cells infected with live P. gingivalis revealed significantly increased production of $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$ at multiplicity of infection of 10. Neutrophils from bone marrow and peritoneal lavage revealed increased production of $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$. Xylitol significantly mitigated P. gingivalis induced cytokine production in neutrophils. Findings indicate that xylitol is an anti-inflammatory agent in neutrophils infected with live P. gingivalis, that suggests its use in periodontitis management.