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http://dx.doi.org/10.11620/IJOB.2018.43.3.141

Xylitol Mitigate Neutrophil Inflammatory Response Against Porphyromonas gingivalis Infection  

Na, Hee Sam (Department of Oral Microbiology, School of Dentistry, Pusan National University)
Song, YuRi (Department of Oral Microbiology, School of Dentistry, Pusan National University)
Choi, Yoon Hee (Department of Hematology-Oncology, Dongnam Institute of Radiological and Medical Sciences)
Chung, Jin (Department of Oral Microbiology, School of Dentistry, Pusan National University)
Publication Information
International Journal of Oral Biology / v.43, no.3, 2018 , pp. 141-146 More about this Journal
Abstract
Periodontitis is generally a chronic disorder characterized by breakdown of tooth-supporting tissues, producing dentition loss. Porphyromonas gingivalis (P. gingivalis), a Gramnegative anaerobic rod, is one of the major pathogens associated with periodontitis. Neutrophils are first line defense cells in the oral cavity that play a significant role in inflammatory response. Xylitol is a known anti-caries agent and has anti-inflammatory effects. In this study, we conducted experiments to evaluate anti-inflammatory effects of xylitol on P. gingivalis infected neutrophils for possible usage in prevention and treatment of periodontal infections. P. gingivalis was intraperitoneally injected and peritoneal lavage was collected for cytokine determination. For in vitro study, neutrophils were collected from mouse peritoneal cells after zymosan injection or bone marrow cells. Neutrophils were stimulated with live P. gingivalis and ELISA was used to determine the effect of xylitol on P. gingivalis induced cytokine production. $IL-1{\beta}$, IL-6, $TNF-{\alpha}$ concentration and neutrophil population in the peritoneal lavage was increased in P. gingivalis-infected mouse. Peritoneal cells infected with live P. gingivalis revealed significantly increased production of $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$ at multiplicity of infection of 10. Neutrophils from bone marrow and peritoneal lavage revealed increased production of $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$. Xylitol significantly mitigated P. gingivalis induced cytokine production in neutrophils. Findings indicate that xylitol is an anti-inflammatory agent in neutrophils infected with live P. gingivalis, that suggests its use in periodontitis management.
Keywords
P. gingivalis; neutrophil; xylitol; inflammation; periodontitis;
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