• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 춘계학술발표회
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• pp.38-38
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• 2018

The genus Carduus (Asteraceae), containing ca. 90 species, is mainly distributed in Eurasia and Africa. Carduus species are one of the most hazardous invasive species, which causes serious environmental threats and biodiversity damages in North America. Thus, the member of Carduus are targeted for classical biological control in this region. Here, we provide the complete cp genome of Carduus crispus using next-generation sequencing technology. The size of cp genomes of C. crispus is 152,342 bp. It shows a typical quadripartite structure, consisting of the large single copy (LSC; 83,254 bp), small single copy (SSC; 18,706 bp), separated by a pair of inverted repeats (IRs; 25,191 bp). It contains 115 unique genes of which 21 genes duplicated in the IR regions. The cpSSR regions of Carduus species were searched through the complete chloroplast genome sequence using a tandem repeat search tool in Geneious with the parameters set to ${\geq}7$ mononucleotide repeats, ${\geq}4$ di- and trinucleotide repeats, and ${\geq}3$ tetra-, penta-, and hexanucleotide repeats. A total of 22 repeat motifs were identified, which may be useful for molecular identification of Korean Carduus species (C. cripus), and providing a guideline for its conservation.

• Natural Product Sciences
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• 제17권4호
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• pp.273-278
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• 2011

In this study, the effects of a methanol (MeOH) extract of Carduus crispus L. (Asteraceae) on adipogenesis was investigated in 3T3-L1 cells. To differentiate preadipocytes to adipocytes, confluent 3T3-L1 preadipocytes were treated with a hormone mixture, which included isobutylmethylxanthine, dexamethasone, and insulin (MDI). The methanol extract of C. crispus significantly decreased fat accumulation by inhibiting adipogenic signal transcriptional factors in MDI-induced 3T3-L1 cells in a dose-dependent manner. In MTT assays and on PI-staining, methanol extract of C. crispus inhibited the proliferation of 3T3-L1 cells during mitotic clonal expansion (MCE). The anti-adipogenic effect of the Carduus extract seemed to be associated with the upregulation of extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (MAPK) pathways within the first 2 days after MDI treatment. These results suggest that methanol extract of C. crispus might be beneficial for the treatment of obesity.

• 한국식품조리과학회지
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• 제25권4호
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• pp.406-414
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• 2009

This study was conducted to investigate the functional composition and antioxidant activity of Cirsium and Carduus genera based on different parts. Leaves of Cirsium setidens Nakai contained 23.66% protein and seeds of Carduus crispus L contained 25.30% lipid. Extraction yields of Cirsium and Carduus genera were higher in leaves than in any other parts of the plants. Total phenolics and total flavonoid content were abundant in extracts of leaves, steam and root of C. japonicum var. ussuriense, and the flower extract of C. setidens Nakai. Silymarin was not found in extracts of Cirsium and Carduus genera. Acacetin was identified in leaf or flower extracts of C. setidens Nakai, or in leaf and steam extracts of C. pendulum Fisch ex DC. Apigenin was identified in the flower extracts of Cirsium and Carduus genera and constituted 7.16 mg/g in C. japonicum var. ussuriense. Cynarin was present at 5.55 mg/g in the seed extract of C. setidens Nakai, and narirutin represented 19.56 mg/g and, 4.18 mg/g of the seed extracts of C. pendulum Fisch ex DC and, Carduus crispus L, respectively. 2,2-Diphenyl-1-2-picrylhydrazyl (DPPH) radical scavenger activity was higher in flower extracts of C. setidens Nakai than in the other genera. Photochemiluminescence activity was 2.3 nmol in leaves and flower extracts of C. setidens Nakai and flower extract of Carduus crispus L. Flowers and seeds of C. setidens Nakai shoe potential as new functional materials.

• 식물분류학회지
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• 제38권2호
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• pp.187-196
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• 2008

우리나라 미기록 귀화식물 2종을 발견하였다. 사향엉겅퀴로 이름 붙인 Carduus natans (국화과)는 경기도 양평군 양수리 및 남양주군 진접읍에서 확인되었으며, 동속 귀화식물인 지느러미엉겅퀴(C. crispus)와 비교하여 두화는 지름이 30-60mm로 보다 크고 대게 하나씩 달리며 고개가 아래로 처지고, 총포편은 창끝모양으로 기부 바로 위가 좁아지는 특징으로 구별된다. 또한 큰키다닥냉이로 이름 붙인 Cepidium latifolium (십자화과)는 키가 1m정도로 크며 잎의 기부가 줄기를 감싸지 않고 하부 줄기의 잎이 우상으로 갈라지며 꽃차례가 짧은 산방상인 특징으로 유사종과 식별된다. 서울 난지도 월드컵 공원에서 처음 확인되었으며 수 백 개체 정도가 발견되어 확산추세에 있는 것으로 판단된다.

• 생명과학회지
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• 제20권4호
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• pp.578-583
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• 2010

세균 및 진균류의 증식을 억제하는 능력이 있는 것으로 보고된 누로와 대계의 추출물을 사용하여 Mycobacterium smegmatis 및 Mycobacterium fortuitum의 증식을 억제하는 능력이 있는지를 시험하였다. 그 결과, 누로의 추출물에서는 증식억제능을 발견할 수 없었으나, 대계의 추출물에서는 뚜렷한 증식억제능이 관찰되었다. 따라서 본 연구에 사용된 대계(엉겅퀴)에 대한 분류학적 또는 진화적 분석을 수행하기 위하여 genomic DNA를 추출한 후, ITS1, 5.8S rRNA 유전자 및 ITS2를 포함하는 부분을 PCR로 증폭시켰다. PCR 산물의 염기서열을 분석한 결과, 733-bp의 염기서열이 얻어졌고, 이것을 GenBank에 등록하였다(accession number GU188570). 이렇게 얻어진 염기서열을 사용하여 BLAST analysis를 수행한 결과, 염기서열이 일치하는 생물체는 아직까지 GenBank에 보고된 적이 없고, 가장 가까운 식물들로는 귀화식물로서 전국적으로 분포하는 Carduus crispus (지느러미엉겅퀴) 및 현재까지 국내에 자생하는 것으로 보고된 적이 없는 Carduus defloratus로서 각각 3개씩의 염기가 다른 것으로 나타났다.

• 대한본초학회지
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• 제28권3호
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• pp.75-84
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• 2013

Objectives : The origin of Breeae Herba (So-gye) and Cirsii Herba (Dae-gye) is differently prescribed in Korean and Chinese modern pharmacopoeia. Since the similar morphological characteristics and chaotic plant names, moreover, the aerial part of Carduus crispus have been used as the Cirsii Herba. To develop a reliable method for correct identification of these herbal medicines and to evaluate the genetic relationship of these closely related plant species, we analyzed sequences of DNA barcode regions. Methods : Thirty-one samples of 6 medicinal plants (B. segeta, B. setosa, C. japonicum var. maackii, C. setidens, C. chanroenicum, and C. crispus) were collected from different habitate and nucleotide sequences of DNA barcode regions (rDNA-ITS, matK, and rbcL) were analyzed after amplification using appropriate primers reported in previous studies. The nucleotides of species-specific authentic marker and phylogenetic relations were estimated based on the entire sequences of DNA barcodes by the analysis of ClastalW and UPGMA, respectively. Results : In comparative analysis of DNA barcode sequences, we obtained specific nucleotides to discriminate the medicinal plant of Breeae/Cirsii Herba in species level and evaluated the phylogenetic relationship of these species. Futhermore, we identified distinct marker nucleotides enough to authenticate respective species. These sequence differences at corresponding positions were avaliable genetic markers to determine the botanical origins of Breeae Herbal as well as Cirsii Herba. Conclusions : These marker nucleotides would be useful to identify the official herbal medicines by providing of definitive information that can identify each plant species and distinguish from unauthentic adulterants and substitutes.

• 한국자원식물학회지
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• 제28권4호
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• pp.511-525
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• 2015

The purpose of this study is to offer the flora data for conserving the biodiversity of Gyeongju National Park by objective surveying and analysing the flora distributed in Mt. Gumi District, Gyeongju National Park, South Korea. The flora of Mt. Gumi were surmmarized as 476 taxa including 97 families, 297 genera, 419 species, 3 subspecies, 46 varieties and 8 forms. The rare plants were 6 taxa such as Aristolochia contorta, Paeonia lactiflora var. trichocarpa, Potentilla discolor, Berchemia berchemiaefolia, Trigonotis icumae and Iris odaesanensis. The Korean endemic plants were 9 taxa such as Carpinus laxiflora, Pseudostellaria coreana, Philadelphus schrenkii, Indigofera koreana, Lespedeza maritima, Vicia chosenensis, Galium koreanum, Lonicera subsessilis and Weigela subsessilis. The specific plants by floristic region were 38 taxa such as Salix chaenomeloides(I), Hylomecon vernalis (I), Oxalis obtriangulata (I), Viola orientalis(II), Adoxa moschatellina (II), Cirsium chanroenicum (II), Vitex negundo var. incisa (III) and so forth. The naturalized plants were 36 taxa such as Fallopia dumetorum, Chenopodium album, Lepidium virginicum, Amorpha fruticosa, Euphorbia supina, Carduus crispus, Erigeron strigosus, Festuca myuros and so forth. The invasive aline plant was Aster pilosus. The target plants adaptable to climate change were 8 taxa such as Artemisia rubripes, Carpesium macrocephalum and so forth.

• 한국자원식물학회지
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• 제17권2호
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• pp.169-182
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• 2004

본 연구의 목적은 충청북도 괴산군에 위치한 박달산(825 m)에 자생하는 관속식물상을 정확하게 파악하고자 수행되었다. 2000년 10월부터 2001년 8월에 걸쳐 조사한 결과 91과 275속 391종 62변종 5품종 등 458종류가 확인되었다. 희귀 및 멸종위기 종은 말나리 , 난장이붓꽃, 매미꽃, 태백제비꽃, 미치광이 풀, 토현삼 등 총 6종류가 조사되었고 특산식물은 가는 장구채, 진범, 매미꽃, 조팝나무, 가시딸기, 주엽 나무, 털조록싸리, 광릉갈퀴, 노랑갈퀴, 뽕잎피나무, 자란초, 오동, 토현삼, 병꽃나무, 청괴불나무, 고려 엉겅퀴, 분취 등 17종류가 관찰되었다. 또한 귀화식물은 애기수영, 소리쟁이, 닭의덩굴, 취명아주, 털비름, 아까시나무, 족제비싸리, 토끼풀, 어저귀, 수박풀, 달맞이꽃, 큰달맞이꽃, 개망초, 망초, 미국가막사리, 지느러미엉겅퀴 등 16종류가 관찰되었다.

• 생약학회지
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• 제39권3호
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• pp.213-217
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• 2008

Oxidative stress is considered to play an important role in a variety of neurodegenerative disorders of central nervous system. The immortalized mouse hippocampal cell line, HT22, phenotypically resembles neuronal precursor cells but lacks functional ionotropic glutamate receptors, thus excluding excitotoxicity as a cause for glutamate triggered cell death. Therefore, HT22 cells are a useful model for studying oxidative glutamate toxicity. In this study, we examined whether the methanol extracts of some native plants at Mt. Baekdu could protect HT22-immortalized hippocampal cells against glutamate-induced oxidative stress. Seventy-eight plants sources were collected at Mt. Baekdu, and extracted with methanol. These extracts had been screened the protective effects against glutamate-induced oxidative damage in HT22 cells at the 100 and 300 ${\mu}g/ml$. Of these, thirteen methanolic extracts, Acer mono (leaf), Artemisia stolonifera (aerial part), Carduus crispus (aerial part), Carex mongolica (whole plant), Clematis hexapetala (whole plant), Galeopsis bifida (aerial part), Galium verum (whole plant), Ganoderma lucidum (whole plant), Ixeris chinensis (whole plant), Malva verticillata (aerial part), Polygonum senticosum (whole plant), Rebes mandshricum (branch), and Taraxacum mongolicum (aerial part), showed significant protective effects against glutamate-induced oxidative damage in HT22 cells.

• Journal of Forest and Environmental Science
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• 제15권1호
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• pp.44-64
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• 1999

향로봉(香爐峰)지역을 대상으로 식물상(植物相)을 조사한 결과, 관속식물(管束植物)은 86과(科) 254속(屬) 393종(種) 2아종(亞種) 48변종(變種) 4품종(品種)으로 총 450종류(種類)가 분포하는 것으로 나타났다. 이 가운데 한국특산식물(韓國特塵植物)은 지리대사초, 키버들, 누른종덩굴, 진범, 금강제비꽃, 산오이풀, 노랑갈퀴, 금마타리, 금강초롱꽃, 흰금강초롱꽃, 각시서덜취 등 23종류(種類), 희귀(稀貴) 및 멸종위기식물(滅種危機植物)은 진부애기나리, 말나리, 연령초, 천마, 누른종덩굴, 도깨비부채, 붉은인가목, 금강제비꽃, 꽃개회나무, 금강초롱꽃 등 17종류(種類)가 분포하고 있었으며, 조사지역내의 귀화식물(歸化植物)로는 오리새, 왕포아풀, 애기수영, 대나물, 다닥냉이, 달맞이꽃, 미국쑥부쟁이, 지느러미엉겅퀴, 개망초, 서양민들레 등 14종류(種類)가 분포하는 것으로 나타났다.