• Journal of Veterinary Science
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• v.24 no.1
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• pp.6.1-6.6
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• 2023

Two Shih-Tzu dogs with atopic dermatitis presented with delayed periocular dermatitis (PD) following the instillation of dorzolamide and dorzolamide/timolol combination eyedrops; the development of dermatologic signs took 94 and 104 d in cases 1 and 2, respectively. Hypersensitivity to anti-glaucoma eyedrops was highly suspected, and treatment was discontinued. Delayed PD was significantly relieved in cases 1 and 2, at days 155 and 64 after discontinuation, respectively. In this study, the clinical characteristics and progression of delayed PD were described to inform clinicians who may encounter this rare side effect.

• Korean Journal of Ecology and Environment
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• v.35 no.3 s.99
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• pp.145-151
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• 2002

The effect of low pH on physiological changes was studied with the acidophilic green alga, Clamydomonas acidophila, UTCC 122. The growthrates (${\mu}$) were identical, $0.5{\sim}0.7\;day^{-1}$, at pH 3.7${\sim}$6.7 and no significantly different (ANOVA, p =0.134), showing cell volume reduced gradually as they were growing, whereas that at pH 2.7 was falling to zero and cell volume increased dramatically. Chlorophyll a concentration of the cultures incubated for one day was $191{\sim}255\;pg\;cell^{-1}$, after then it declined from $60{\sim}103\;pg\;cell^{-1}$ at pH 3.7${\sim}$6.7 except $210\;pg\;cell^{-1}$ at pH 2.7, which was directly related with cell volume. External carbonic anhydrase (CA) activity was varied from1.1 to$3.7{\times}10^{-4}\;E.U.\;mm^{-2}$, showing the gradualincrease during culture, except at 2.7 and pH 5.7. However there was not found any relationship among the pH gradient cultures. CA molecular mass of C. acidophila was 29 kBa, and concentration of that was identical in all cultures. The proteins of 41 kDa and 63 were not or very　faintly expressed in low pH cultures, in contrast that of 17 kDa more expressed. In this work, we found that C. acidophila could live optimally within a wide range of acidic pH, and 17 kDa of unidentified protein might be concerned with tolerating in low acid environment.

• Korean Journal of Veterinary Research
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• v.12 no.1
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• pp.37-50
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• 1972

Another comparison of alveolar gas pressures, in a continuously rebreathing dog lung, with pulmonary arterial (mixed venous) blood, again showed alveolar pressures to be the higher for $CO_2$ and usually $O_2$ (positive ${\Delta}Pco_2$, and ${\Delta}Po_2$). ${\Delta}Pco_2$ was almost invariably positive, it was independant of time and of plasma pH changes produced by acid or bicarbonate infusion, but proportional to blood $CO_2$ level. It was seemingly little affected by the distribution or magnitude of blood flow nor did it result from failure of $CO_2$ equilibrium within the blood, but it did rise with carbonic anhydrase inhibition. ${\Delta}Po_2$ was more variable and sometimes negative, particularly at low blood flow rates. It seemed in dependant of time, and plasma pH changes, apart from an inverse relationship with that resulting from $CO_2$ changes. It existed despite considerable potential for gas exchange, and was unaffected by inhibition of the $CO_2$ reactions.

• Applied Microscopy
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• v.23 no.1
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• pp.1-14
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• 1993

The present study was carried out to analyze the evidence of membrane recycling, and the regulation of cellular transport by dynamic changes in apical membrane area that functionally interacts with the number of cytoplasmic vesicles. Under scanning electron micrographs, turtle bladder mucosa contain three main type of cells; granular cells and carbonic anhydrase (CA)-rich cells, deviding into a and b type of epithelial cell. The granular cell is the majority cell type of the mucosa comprising 80% of the total cell number. The remaining 20% of the cells are characteristically rich in carbonic anhydrase. Uptake of HRP was detected in the most vacuoles or tubulovesicles in both type of CA-rich cells in the turtle bladder, indicating that the part of plasma membrane was internalized in the apical cytoplasmic vacuoles. It seems quite likely that CA-rich cells possess intracellular vesicles carrying proton pumps which are recycling back to the apical plasma membrane. In turtle bladder, the granular cells actively secrete large quantities of mucin and other proteins by an exocytotic mechanism in an apparently constitutive fashion. The possibility that bladder epithelial cells secrete mucin via a regulated secretory pathway has not been rigorously examined and much is still to be determined about these issues from this cell type.

• Tuberculosis and Respiratory Diseases
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• v.63 no.1
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• pp.52-58
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• 2007

Background: Photodynamic therapy is a viable option for lung cancer treatment, and many studies have shown that it is capable of inducing cell death in lung cancer cells. However, the precise mechanism of this cell death has not been fully elucidated. To investigate the early changes in cancer cell transcription, we treated A549 cells with the photosensitizer DH-I-180-3 and then we illuminated the cells. Methods: We investigated the gene expression profiles of the the A549 lung cancer cell line, using a DEG kit, following photodynamic therapy and we evaluated the cell viability by performing flow cytometry. We identified the genes that were significantly changed following photodynamic therapy by performing DNA sequencing. Results: The FACS data showed that the cell death of the lung cancer cells was mainly caused by necrosis. We found nine genes that were significantly changed and we identified eight of these genes. We evaluated the expression of two genes, 3-phosphoglycerate dehydrogenase and ribosomal protein S29. The expressed level of carbonic anhydrase XII, clusterin, MRP3s1 protein, complement 3, membrane cofactor protein and integrin beta 1 were decreased. Conclusion: Many of the gene products are membrane-associated proteins. The main mechanism of photodynamic therapy with using the photosensitizing agent DH-I-180-3 appears to be necrosis and this may be associated with the altered production of membrane proteins.

• Korean Journal of Veterinary Research
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• v.25 no.1
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• pp.19-25
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• 1985

한국(韓國), 대만(臺灣), 일본(日本)에서 사육(飼育)하고 있는 꽃사슴(Cervus nippon) 129두(頭)와 물사슴(Cervus unicolor) 7두(頭)에서 hemoglobin, transferrin, albumin carbonic anhydrase, slow-${\alpha}_2$ 및 amylase형(型)을 전기영동(電氣泳動)에 의(依)하여 분석(分析)한 결과(結果) 다음과 같은 결론(結論)을 얻었다. 1. cellulose acetate에 의(依)한 전기영동(電氣泳動)이 starch gel에 의(依)한 전기영동(電氣泳動)보다 hemoglobin형(型) 분리(分離)에 있어서 더 간편하며 시간이 적게 걸리고, 선명(鮮明)할 뿐만 아니라 영구보존(永久保存)이 가능(可能)하다. 2. 꽃사슴의 hemoglobin형(型)은 $Hb^F$, $Hb^{FS}$, $Hb^S$형(型)으로 분리(分離)되었으나 물사슴에 있어서는 전부(全部) $Hb^F$형(型)으로 나타났다. 3. hemoglobin ${\beta}$ chain은 4가지형(型) 즉 ${\beta}$-1, ${\beta}$-2, ${\beta}$-3 및 ${\beta}$-4로 분리(分離)되었다. 4. hemoglobin ${\alpha}$ chain은 ${\alpha}_1$과 ${\alpha}_1{\alpha}_2$형(型)으로 분리(分離)되었다. 5. slow-${\alpha}_2$형(型)은 A형(型)과 AB형(型)으로 분리(分離)되었으며, 꽃사슴에 있어서는 AB형(型)이 12% 출현(出現)하였으나 물사슴에서는 전부(全部) A형(型)으로 AB형(型)은 없었다. 6. albumin형(型)에서는 F형(型)과 S형(型)으로 분리(分離)되었으며 꽃사슴은 전부(全部) F형(型)이였고, 물사슴은 전부(全部) S형(型)이였다. 7. transferrin형(型), carbonic anhydrase형(型) 및 amylase형(型)은 전부(全部) 각각(各各) 1종류(種類)의 형(型)이였다.

• Bulletin of the Korean Chemical Society
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• v.33 no.10
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• pp.3233-3240
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• 2012

We demonstrated the combined applications of online protein digestion using trypsin immobilized enzyme reactor (IMER) and dual tandem mass spectrometry with collisionally activated dissociation (CAD) and electron transfer dissociation (ETD) for tryptic peptides eluted through the trypsin-IMER. For the trypsin-IMER, the organic and inorganic hybrid monolithic material was used. By employing the trypsin-IMER, the long digestion time could be saved with little or no sacrifice of the digestion efficiency, which was demonstrated for standard protein samples. For three model proteins (cytochrome c, carbonic anhydrase, and bovine serum albumin), the tryptic peptides digested by the IMER were analyzed using LC-MS/MS with the dual application of CAD and ETD. As previously shown by others, the dual application of CAD and ETD increased the sequence coverage in comparison with CAD application only. In particular, ETD was very useful for the analysis of highly-protontated peptide cations, e.g., ${\geq}3+$. The combination approach provided the advantages of both trypsin-IMER and CAD/ETD dual tandem mass spectrometry applications, which are rapid digestion (i.e., 10 min), good digestion efficiency, online coupling of trypsin-IMER and liquid chromatography, and high sequence coverage.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.10
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• pp.1398-1402
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• 2002

Hu sheep was sampled randomly from Huzhou city, Zhejiang province, China. Of the 11 genetic markers from the blood examined by starch-gel and cellulose acetate electrophoresis, polymorphisms in Hu sheep were found for 10 loci, i.e. post-albumin (Po), transferring (Tf), alkaline phosphatase (Alp), leucine aminopeptidase (Lap), arylesterase (Ary-Es), hemoglobin-$\beta$ (Hb-$\beta$)、Xprotein(X-p), carbonic anhydrase (CA), catalase (Cat) and lysine (Ly). The same data except for Po locus were collected from another 14 sheep breeds from China and other countries, in order to ascertain their genetic relationships with one another and with the Hu sheep. The sheep populations from the east and south of Central Asia can be classified into three genetic groups: 'Mongolian sheep', 'South Asian sheep' and 'European sheep'. The Hu sheep belong to the 'Mongolian sheep' group.

• Molecules and Cells
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• v.25 no.3
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• pp.446-451
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• 2008

We assessed heterologous protein expression in 64 strains obtained from the Escherichia coli Reference (ECOR) collection, a collection representing diverse natural E. coli populations. A plasmid generating a glutathione S-transferase and plant carbonic anhydrase fusion protein (GST-CA) under the control of the tac promoter was introduced into the ECOR strains, and the quantity of the fusion protein was determined by SDS-PAGE. The foreign protein was generated at various levels, from very high (40 strains, high producers) to very low (six strains, low producers). Immunoblotting showed that the high producers expressed approximately 250-500 times more GST-CA protein than the low producers. The results of semi-quantitative RT-PCR showed that the low producers generated mRNA levels comparable to those of the high producers, thereby suggesting that, at least in this case, inefficient translation is a major cause of the low production. We introduced a different plasmid, which expressed a maltose binding protein and plant guanylate kinase fusion protein (MBP-GK) into the six low producers. Interestingly, five of these expressed MBP-GK at very high levels. Thus, we conclude that the production of a particular protein from an expression vector can vary considerably, depending on the host strain. Strains in the ECOR collection could function as useful alternative hosts when a desired level of protein expression is not obtained from commonly used strains, such as E. coli K12 or B derivatives.

• Journal of Microbiology and Biotechnology
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• v.20 no.10
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• pp.1424-1429
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• 2010

A poly(${\gamma}$-glutamic acid) (${\gamma}$PGA)-cholesterol conjugate was synthesized and its properties were then evaluated. The conjugate exhibited an amphiphilic nature derived from the hydrophilic ${\gamma}$PGA backbone and the hydrophobic cholesterol side chain. The conjugate spontaneously formed nanoparticles, becoming an aqueous solution when at low concentrations, and at high concentrations the result was the formation of a physical gel. By utilizing the self-aggregating properties of the conjugate in water, an artificial chaperone was developed. A complex of protein, with the nanoparticles of the conjugate, was formed and the protein was released upon the dissociation of the nanoparticles through the addition of ${\beta}$-cyclodextrin. For denatured carbonic anhydrase, the activity was recovered in the artificial chaperone of the nanoparticle conjugate.