• Journal of Bio-Environment Control
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• v.21 no.1
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• pp.50-56
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• 2012

This study was conducted to extend the postharvest life of eggplant ($Solanum$ $melongena$ L.) by modified atmosphere packaging using a OPP bag with 1-MCP to evaluate the alleviation effects of that treatment on external chilling injury during at low temperature. 1-MCP treatment at the level of $1.0{\mu}L{\cdot}L^{-1}$ were very effective in delaying the progress of surface bronzing of eggplant during 10 days of storage at $8^{\circ}C$, but not significant at $1^{\circ}C$. Also, the treatment of 1-MCP at the levels of $0.1{\sim}1.0{\mu}L{\cdot}L^{-1}$ are beneficial to complement low temperature storage and maintain quality indices such as higher firmness and lower weight loss than untreated control even at a critical low temperature 4 to $8^{\circ}C$. The treatment of carbon dioxide using dry ice (DI, 5 g/3 fruits) significantly promoted chilling injury of eggplant stored at $4^{\circ}C$, symptoms were appeared from 3 days after treatment and combination treatment of 1-MCP did not block the development of chilling injury symptom. Meanwhile, single treatment of $CO_2$ absorber (CA, 5 g/3 fruits) or combination treatment with 1-MCP at the level of $1.0{\mu}L{\cdot}L^{-1}$ prevented effectively the external and internal chilling injury at $4^{\circ}C$ during 6 days and 15 days of MAP storage, respectively. Results suggest that 1-MCP and carbon dioxide absorber treatments delay fruit deterioration and are beneficial to broaden storage temperature of eggplant fruits.

• Proceedings of the Korean Society of Life Science Conference
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• 2001.11a
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• pp.3-8
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• 2001

Isopentenyl diphosphate (IPP) is the common, five-carbon building block in the biosynthesis of all carotenoids, IPP in Escherichia coli is synthesized through the non-mevalonate pathway. The first reaction of IPP biosynthesis in E. coli is the formation of 1-deoxy-D-xylulose-5-phosphate (DXP), catalyzed by DXP synthase and encoded by dxs. The second reaction in the pathway is the reduction of DXP to 2-C-methyl-D-erythritol-4-phosphate, catalyzed by DXP reductoisomerase and encoded by dxr. To determine if one or more of the reactions in the non-mevalonate pathway controlled flux to IPP, dxs and dxr were placed on several expression vectors under the control of three different promoters and transformed into three E. coli strains (DH5(, XL1-Blue, and JM101) that had been engineered to produce lycopene. Lycopene production was improved significantly in strains transformed with the dxs expression vectors. When the dxs gene was expressed from the arabinose-inducible araBAD promoter (PBAD) on a medium-copy plasmid, lycopene production was 2-fold higher than when dxs was expressed from the IPTG-inducible trc and lac promoters (Ptrc and Plac, respectively) on medium-copy and high-copy plasmids, Given the low final densities of cells expressing dxs from IPTG-inducible promoters, the low lycopene production was probably due to the metabolic burden of plasmid maintenance and an excessive drain of central metabolic intermediates. At arabinose concentrations between 0 and 1.33 mM, cells expressing both dxs and dxr from PBAD on a medium-copy plasmid produced 1.4 - 2.0 times more lycopene than cells expressing dxs only. However, at higher arabinose concentrations lycopene production in cells expressing both dxs and dxr was lower than in cells expressing dxs only. A comparison of the three E. coli strains transformed with the arabinose-inducible dxs on a medium-copy plamid revealed that lycopene production was highest in XL1-Blue.

• Journal of Microbiology and Biotechnology
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• v.16 no.7
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• pp.1032-1040
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• 2006

Some Pseudomonas species can grow on styrene as a sole carbon and energy source. From the new isolate Pseudomonas putida SN1, the genes for styrene catabolism were cloned and sequenced. They were composed of four structural genes for styrene monooxygenase (styA and styB), styrene oxide isomerase (styC), and phenylacetaldehyde dehydrogenase (styD), along with two genes for the regulatory system (styS and styR). All the genes showed high DNA sequence (91% to 99%) and amino acid sequence (94% to 100%) similarities with the corresponding genes of the previously reported styrene-degrading Pseudomonas strains. A recombinant Escherichia coli to contain the styrene monooxygenase from the SN1 was constructed under the control of the T7 promoter for the production of enantiopure (S)-styrene oxide, which is an important chiral building block in organic synthesis. The recombinant E. coli could convert styrene into an enantiopure (S)-styrene oxide (ee >99%) when induced by IPTG The maximum activity was observed as 140 U/g cell, when induced with 1 mM IPTG at $15^{\circ}C$.

• Journal of Ocean Engineering and Technology
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• v.24 no.6
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• pp.71-75
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• 2010

Although Graphite Compacted Vermicular (GCV) was first observed in 1948, the narrow range for stable foundry production precluded the high volume application of GCV to complex components such as cylinder blocks and heads until advanced process control technologies became available. This, in turn, had to await the advent of modern measurement electronics and computer processors. Following the development of foundry techniques and manufacturing solutions, primarily initiated in Europe during the 1990s, the first series production of GCV cylinder blocks began during 1999. Today, more than 40,000 GCV cylinder blocks are produced each month for OEMs, including Audi, DAF, Ford, Hundai, MAN, Mercedes, PSA, Volkswagen, and Volvo. Given that new engine programs are typically intended to support three to four vehicle generations, the chosen engine materials must satisfy current design criteria and also provide the potential for future performance upgrades without changing the overall block architecture. With at least a 75% increase in the ultimate tensile strength, a 40% increase in the elastic modulus, and approximately double the fatigue strength of either iron or aluminum, GCV is ideally suited to meet current and future of engine design and performance requirements.

• Journal of Ocean Engineering and Technology
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• v.24 no.6
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• pp.76-80
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• 2010

Oil hydraulic piston pumps are being extensively used around the world, because of their simple design, light weight, effective cost, etc. An oil hydraulic pump is likely to have the serious problems of high leakage, friction, and low energy efficiency after a long period of use. In an oil hydraulic piston pump, the clearance between the valve block and piston plays an important role for volumetric and overall efficiency. In this study, the wear property of the SACM645 material with DLC coating used for a hydraulic piston pump was determined by experimentation with variable heat treatment. To investigate the effect according to the piston surface condition, five different types of specimens were prepared. The maximum tensile strengths of the QT and QT Nitration specimens had similar values of about 800 MPa, but the strains indicated a big difference. In a wear test, the wear characteristic of the DLC coating specimen was shown to be excellent. The QT, QT + IH, QT + Nitration, and matirx specimen showed similar wear characteristics. In the case of a dry condition without oil, the DLC coating specimen had good wear resistance, with no wear shown.

• Applied Chemistry for Engineering
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• v.24 no.4
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• pp.382-390
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• 2013

Foaming process of waste soda lime glasses by just chemical composition control of vitreous feed materials was investigated to find a novel and efficient recycling process. For the chemical composition control of feed materials, 10 wt. parts of $SiO_2$, 0.5 wt. parts of $Na_2SO_4$, 3.0 wt. parts of $B_2O_3$, and 0.3 wt. parts of carbon black as the foaming agent were mixed with 100 wt. parts of soda-lime vitreous feed powder. Proper conditions for foaming process in tunnel kiln are the foaming temperature of $830{\sim}850^{\circ}C$, the foaming time of 30~35 min, and the vitreous feed powder particle size of -325 mesh. Properties of foamed glass blocks obtained under these foaming conditions showed the density of $0.17{\sim}0.21g/cm^3$, thermal conductivity of $0.06{\pm}0.005kcal/h{\cdot}m{\cdot}^{\circ}C$, moisture absorption of 1.1~1.5%, and compressive strength of $20{\sim}30kgf/mm^2$.

• Journal of the Korean Ceramic Society
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• v.35 no.2
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• pp.172-179
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• 1998

Valve lifter namely tappet is supported by lifter hole which is located upper side of camshaft in cylinder block transforms rotatic mvement of camshaft into linear movement and helps to open and shut the en-gine valve as an engine parts. The face of valve lifter which is continuously contacting with camshaft brings about abnormal wears such as unfair wear and early wear because it is severely loaded in the valve train system. These wears act as a defect like over-clearance and cause imperfect combustion of fuel during the valve lifting in the combustion chamber. Consequently this imperfect combustion makes the engine out-put decrease and has cause on air pollution. To prevent these wears therefore The valve lifter cast in me-tal developed into SiC ceramics valve lifter which has an excellence in wear and impact resistance As a results the optimum process conditions like injection condition mixture ratio and debonding process could be established. After sintering fine-sinered dual microstructure in which prior ${\alpha}$-SiC matches well with new SiC(${\beta}$-SiC) produced by reaction among the ${\alpha}$-SiC carbon and silicon was obtained. Based on the study it is verified that mechanical properties of SiC valve lifter are excellent in Vickers hardness 1100-1200 bending strength (300-350 Pa) fracture toughness(1.5-1.7 Mpa$.$m1/2) Through engine dynamo test-ing SiC valve lifter and metal valve lifter are examined and compared into abnormal phenomena such as early fracture unfair and early wear. It is hoped that this research will serve as an important springboard for the future study of heavy duty diesel engine parts developed by ceramics which has a good wear resis-tance relaibility and lightability.

• Steel and Composite Structures
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• v.7 no.6
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• pp.457-468
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• 2007

Analytical and experimental investigation on dynamic properties of extra lightweight concrete sandwich beams reinforced with various lay ups of carbon reinforced epoxy polymer composites (CFRP) are discussed. The lightweight concrete used in the core of the sandwich beams was made up of extra lightweight aggregate, Lica. The density of concrete was half of that of the ordinary concrete and its compressive strength was about $100Kg/cm^2$. Two extra lightweight unreinforced (control) beams and six extra lightweight sandwich beams with various lay ups of CFRP were clamped in one end and tested under an impact load. The dimension of the beams without considering any reinforcement was 20 cm ${\times}$ 10 cm ${\times}$ 1.4 m. These were selected to ensure that the effect of shear during the bending test would be minimized. Three other beams, made up of ordinary concrete reinforced with steel bars, were tested in the same conditions. For measuring the damping capacity of sandwich beams three methods, Logarithmic Decrement Analysis (LDA), Hilbert Transform Analysis (HTA) and Moving Block Analysis (MBA) were applied. The first two methods are in time domain and the last one is in frequency domain. A comparison between the damping capacity of the beams obtained from all three methods, shows that the damping capacity of the extra lightweight concrete decreases by adding the composite reinforced layers to the upper and lower sides of the beams, and becomes most similar to the damping of the ordinary beams. Also the results show that the stiffness of the extra lightweight concrete beams increases by adding the composite reinforced layer to their both sides and become similar to the ordinary beams.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2001.06a
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• pp.141-145
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• 2001

Isopentenyl diphosphate (IPP) is the common, five-carbon building block in the biosynthesis of all carotenoids. IPP in Escherichia coli is synthesized through the non-mevalonate pathway. The first reaction of IPP biosynthesis in E. coli is the formation of l-deoxy-D-xylulose-5-phosphate (DXP), catalyzed by DXP synthase and encoded by dxs. The second reaction in the pathway is the reduction of DXP to 2-C-methyl-D-erythritol-4-phosphate, catalyzed by DXP reductoisomerase and encoded by dxr. To determine if one or more of the reactions in the non-mevalonate pathway controlled flux to IPP, dxs and dxr were placed on several expression vectors under the control of three different promoters and transformed into three E. coli strains (DH5$\alpha$, XL1-Blue, and JMl0l) that had been engineered to produce lycopene. Lycopene production was improved significantly in strains transformed with the dxs expression vectors. When the dxs gene was expressed from the arabinose-inducible araBAD promoter ( $P_{BAD}$) on a medium-copy plasmid, lycopene production was 2-fold higher than when dxs was expressed from the IPTG-inducible trc and lac promoters ( $P_{trc}$ and $P_{lac}$, respectively) on medium-copy and high-copy plasmids. Given the low final densities of cells expressing dxs from IPTG-inducible promoters, the low lycopene production was probably due to the metabolic burden of plasmid maintenance and an excessive drain of central metabolic intermediates. At arabinose concentrations between 0 and 1.33 roM, cells expressing both dxs and dxr from $P_{BAD}$ on a medium-copy plasmid produced 1.4 - 2.0 times more lycopene than cells expressing dxs only. However, at higher arabinose concentrations lycopene . production in cells expressing both dxs and dxr was lower than in cells expressing dxs only. A comparison of the three E. coli strains transformed with the arabinose-inducible dxs on a medium-copy plasmid revealed that lycopene production was highest in XLI-Blue.LI-Blue.

• 한국신재생에너지학회:학술대회논문집
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• 2011.11a
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• pp.114.2-114.2
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• 2011

Furfural is a versatile derivative. It can be utilized for a building-block of furfuryl alcohol production and a component of fuels or liquid alkanes. But in bio-process, furfural is a critical compound because it inhibits cell growth and metabolism. Furfural could be converted from xylose and usually produced from biomass in which hemicellulose is abundant. In this study, furfural production from miscanthus was performed and utilization of miscanthus residue was consequently conducted. At first, hydrolysis for investigation of miscanthus composition and furfural production was performed using sulfuric acid. Previously, we optimized dilute acid pretreatment condition for miscanthus pretreatment and the condition was found to be about 15 min of reaction time, 1.5% of acid concentration and about $140^{\circ}C$ of temperature and 60% (about 7 g/L) of xylose was solubilized from miscanthus. Using the xylose, furfural production was conducted as second step. Approximately $160{\sim}200^{\circ}C$ of temperature was accompanied with the hydrolysis for pyrolysis of biomass. When the investigated condition; $180^{\circ}C$ of temperature, 20 min of reaction time and 2% of acid concentration was operated for furfural production, furfural productivity was reached to be 77% of theoretical maximum. After reaction, residue of miscanthus was utilized as feedstock of ethanol fermentation. Residue was well washed using water and saccharified using hydrolysis enzymes. Hydrolysate (glucose) from saccharification was utilized for the carbon source of Saccharomyces cervisiae K35.