Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Characterization of Styrene Catabolic Genes of Pseudomonas putida SN1 and Construction of a Recombinant Escherichia coli Containing Styrene Monooxygenase Gene for the Production of (S)-Styrene Oxide

  • Park Mi-So (Department of Chemical and Biochemical Engineering,Pusan National University) ;
  • Bae Jong-Won (Department of Chemical and Biochemical Engineering,Pusan National University) ;
  • Han Ju-Hee (Department of Chemical and Biochemical Engineering,Pusan National University) ;
  • Lee Eun-Yeol (Institute for Environmental Technology and Industry, Pusan National University) ;
  • Lee Sun-Gu (Department of Chemical and Biochemical Engineering,Pusan National University) ;
  • Park Sung-Hoon (Department of Chemical and Biochemical Engineering,Pusan National University)
  • Published : 2006.07.01
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Abstract

Some Pseudomonas species can grow on styrene as a sole carbon and energy source. From the new isolate Pseudomonas putida SN1, the genes for styrene catabolism were cloned and sequenced. They were composed of four structural genes for styrene monooxygenase (styA and styB), styrene oxide isomerase (styC), and phenylacetaldehyde dehydrogenase (styD), along with two genes for the regulatory system (styS and styR). All the genes showed high DNA sequence (91% to 99%) and amino acid sequence (94% to 100%) similarities with the corresponding genes of the previously reported styrene-degrading Pseudomonas strains. A recombinant Escherichia coli to contain the styrene monooxygenase from the SN1 was constructed under the control of the T7 promoter for the production of enantiopure (S)-styrene oxide, which is an important chiral building block in organic synthesis. The recombinant E. coli could convert styrene into an enantiopure (S)-styrene oxide (ee >99%) when induced by IPTG The maximum activity was observed as 140 U/g cell, when induced with 1 mM IPTG at $15^{\circ}C$.

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References

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