• 한국산업융합학회 논문집
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• 제7권1호
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• pp.107-113
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• 2004

Several transition metal complexes, [$M(L)X_2$](M=Pd(II), Ni(II); X=CI, Br) are prepared with aminophosphine ligands such as 1,2-bis{(diphenylphosphino)amino}ethane{$Ph_2PNHCH_2CH_2NHPPh_2$}($L_1$), 1,2-bis{(diphenylphosphino)amino}propane{$Ph_2PNHCH(CH_3)CH_2NHPPh_2$}($L_2$), trans-1,2-bis{(diphenylphosphino)amino}cyclohexane{$Ph_2PNHC_6H_{10}NHPPh_2$}($L_3$) and 1,2-bis{(diphenylphosphino)amino}benzene{$Ph_2PNHC_6H_4NHPPh_2$}($L_4$). The properties of these complexes are characterized by optical spectroscopic methods including UV/vis spectroscopy, CD, IR, $^1H$- and $^{31}P-NMR$ together with conductometer and elemental analysis. All complexes are stable under atmospheric environment. Catalytic reactivity for C-C coupling between [$M(L)X_2$] and Grignard reagents(RMgX; R=phenyl, propyl, buthyl) by thermolysis were investigated utilizing GC/mass, $^1H$- and $^{13}C-NMR$. When mol scale is 1:20 at [$Pd(L)Cl_2$] and Grignard reagents, the high catalytic activity for C-C coupling is apparent. The [$M(L)X_2$](X=Cl, Br) complexes which have strong bond at M-P exhibit high yields for C-C coupling reactions. When the central metal ion is Pd(II), the high catalytic activity for C-C coupling is apparent. The complex coordinated with Br shows higher catalytic activity for C-C coupling reactions compared to Cl.

• 대한화학회지
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• 제27권2호
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• pp.117-126
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• 1983

탄소-13 및 바나듐-51 핵자기공명분광법을 이용하여 아미노폴리카르본산-바나듐(v) 착물의 용액 내에서의 구조에 대해 연구하였다. 이 때 사용한 리간드는 ethylenediaminetetraacetic acid (EDTA), trans-cyclohexanediaminetetraacetic acid (CDTA), 1,2-propylenediaminetetraacetic acid (PDTA), ethylenediaminediacetic acid (EDDA), 2-hydroxyethylethylenediaminetriacetic acid (HEDTA), diethylenetriaminepentacetic acid (DPTA), nitrilotriacetic acid (NTA) 등이었다. 모든 리간드는 바나듐(Ⅴ)과 시스-VO2핵을 함유하는 정8면체 구조의 1:1 착물을 생성하였다. 바나듐(V)과 시스-$VO_2$ 핵을 함유하는 정8면체 구조의 1:1 착물을 생성하였다. 바나듐(V)에 대한 히드록시에틸기의 배위는 아세테이트기의 배위에 비해 덜 바람직함을 알 수 있었다. 그리고 EDDA 및 PDTA는 각각 2가지의 이성체착물들을 생성함을 알 수 있었다.

• 한국응용과학기술학회지
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• 제22권4호
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• pp.323-331
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• 2005

Fatty acid compositions of the seed oils of P. schinseng, A. continentalis and A. sessiliflorus, were analyzed by gas chromatography (GC) equipped with a capillary column. A large unusual peak was observed just before the peak corresponding to oleic acid $(cis-9-C_{18:1})$. This unknown fatty acid was isolated by silver ion chromatography and then derivatized into the picolinyl ester. The mass spectrum of the picolinyl ester showed molecular ion at m/z=373 with other diagnostic ions such as m/z=178, 218, 232, 246, 274, 288, 302 and 344. Characteristic absorption peaks at $720\;cm^{-1}$, $1640\;cm^{-1}$ and $3010\;cm^{-1}$ in IR spectrum indicated the presence of cis-configurational double bond in the molecule. The $^1H-NMR$ spectrum of this acid gave two quintets centered at ${\delta}1.638$ (2H, C-3) and ${\delta}1.377$ (2H, C-4), and two multiplets centered at ${\delta}2.022{\sim}2.047$ (2H, C-5) and ${\delta}2.000{\sim}2.022$ (2H, C-8), and multiplet signals of olefinic protons centered at ${\delta}5.3015{\sim}5.3426$ (C-6, J=9.5 Hz) and ${\delta}\;5.3465{\sim}5.3877$ (C-7, J=9.5 Hz). The $^13C-NMR$ spectrum showed 18 carbon resonance signals including an overlapped signal at ${\delta}29.7002$ for C-12 and ${\delta}29.6520$ for C-13 (or they can be reversed), and other highly resolved signals at ${\delta}33.950$, ${\delta}24.558$, ${\delta}26.773$ and ${\delta}27.205$ due to C-2, C-3, C-5 and C-8 of a ${\Delta}^6-octadecenoic$ acid, respectively. From analysis results this unknown fatty acid could be identified as cis-6-octadecenoic acid. The seed oils of P. schinseng and A. sessiliflorus contained petroselinic acid (59.7%, 56.0%), oleic acid (18.3%, 6.1%) and linoleic acid (16.2%, 30.4%) with small amount of palmitic acid (3.0%, 3.1%) while the seed oil of A. continentalis comprised mainly oleic acid (30.2%), petroselinic acid (29.0%), linoleic acid (24.1%) and palmitic acid (13.1%).

• 한국미생물·생명공학회지
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• 제30권1호
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• pp.51-56
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• 2002

국내 토양에서 분리한 식물성 병원균인 Alternaria brassicicola SW-3리 항암활성 물질 생산능을 조사하고, 활성물질을 분리 정제하여 구조를 확인하였다. A. brassicicola SW-3는potato dextrose broth를 이용하여 15$^{\circ}C$에서 2주간 진탕 배양한 다음, MTT assay를 실시하여 항암활성을 확인하였으며, 배양여액 중의 항암물질은 ethyl acetate로 추출하고, silica gel column chromatography로 정제하여 무색의 oily product를 얻었다(수율 22mg/m1). 분리된 물질은 물이나 hexane에는 녹지 않고, chloroform, ethyl acetate, ethanol 등에는 잘 녹는 특징을 보였으며, , $IR^{1}$H-NMR, $^{13}$C-NMR 등을 통해 구조를 분석한 결과, 최근에 일본에서 분리되어 항암효과가 있는 것으로 알려진 depudecin과 동일한 물질로 추정되었다. 본 실험에서 분리된 depudecin은 인체간암세포와 mouse 피부암세포에 대한 세포독성을 나타내었으며, 각각의$ IC_50$ 는 $57\mu$g/ml, $69\mu$g/ml로 나타났다. Alternaria brassicicola SW-3에 의해 생산된 물질이 기지의 물질이지만, depudecin은 아직 작용 기작이나 적용범위 등이 밝혀지지 않은 초기 연구 단계에 있는 물질로서, 새로운 항암제로서의 가능성이 매우 높아 이의 유도체를 합성하거나, 다른 항암제와의 혼용에 의해 부작용이 적은 강력한 항암제를 개발하기 위한 선도물질로 활용될 수 있을 것이다.

• 한국미생물·생명공학회지
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• 제18권3호
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• pp.273-279
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• 1990

PHB 생산을 위하여 메탄올을 기질로 한 선별배지에서 토양, 하천수, 퇴비 등으로부터 분홍색 색소를 가지는 PHB 축적 facultative methylotroph를 분리하여, 균주의 특성을 검토하였다. 분리균주의 최적 생육조건과 PHB 축적을 위한 배양조건을 조사한 결과 균체의 생육은 메탄올 농도 1.0(v/v), 질소원인$ NH_4C$ 농도 1.0g/l, 즉 C/N ratio 13.2 일때 그리고 pH 7.0과'$30^{\circ}C$에서 가장 좋았으며, PHB는 C/N ratio가 50.8, 즉 메탄올 농도 1.0(v/v )$NH_4CL$ 0.26g/l 일때, 그리고 pH 6.0일 때 건조중량의 약 40까지 축적되었다. 고농도 메탄올에 의한 생육저해를 극복하기 위하여 기질을 간헐적으로 계속 공급해주는 fed-batch 배양을 시도한 결과 균체량은 14g/l, PHB 축척량은 5.5g/l까지 증가시킬 수 있었다. 생산된 PHB를 분리.정제하여 IR과 $^I H-NMR$로 구조를 분석한 결과 3-hydroxybutyric acid 의 homopolymer임이 확인되었다. 또한 균주의 pink-pigment를 추출하여 absorption spectrum를 조사하여 그 특성을 규명하였다.

• 한국식품영양학회지
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• 제11권5호
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• pp.499-505
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• 1998

The purpose of this study was to ivestigate the structural characteristics of waste soulble browning reaction prodcuts (WS-BRPs) isolated from Korean red ginseng. They all showed the maximum absorbances at near 280 nm. Their IR spectra suggested the presence of hydroxyl, amide carbonyl and aliphatic methine groups. From sugar analysis it was identified that L and S-1 contained two kinds of suggar, glucose and xylose, and S-2, a fructose together with glucose and xylose. Thirteen different amino acids were identified in L. Ten amino acids from S-1 and seven amino acids from S-2 were identified using Auto Amino Acid Analyzer. Glycine, serine and glutamic acid in S-1 and one unknown amino acid and glycine in S-2 were detected as the major amino acids, respectively. From the 1H-and 13C-NMR spectra, it was identified that a number of sugar moieties, carbonyl and carbon double bonds (only in S-2) were contained in the three WS-BRP components. Approximate number of sugar moiety of L, S-1 and S-2 was determined to be 8∼10, 9∼11 and 4∼5, respectively. Contents of nitrogen, carbon and hydrogen showed L>S-1>S-2.

• Carbon letters
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• 제12권4호
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• pp.229-235
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• 2011

Isotactic polyacrylonitrile (PAN) with triad isotacticity of 0.53, which was determined by $^{13}C$ NMR, using dialkylmagnesium as an initiator, was successfully synthesized. Isothermal treatment of iso-PAN was conducted in air at 200, 220, 250 and $280^{\circ}C$. Structural evolutions and chemical changes were studied with Fourier transformation infrared and wide-angle X-ray diffraction during stabilization. A new parameter $CNF={I_{2240cm}}^{-1}/ ({I_{1595cm}}^{-1}+f^*{I_{1595cm}}^{-1})$ was defined to evaluate residual nitrile groups. Crystallinity and crystal size were calculated with X-ray diffraction dates. The results indicated that the nitrile groups had partly converted into a ladder structure as stabilization proceeded. The rate of reaction increased with treatment temperature; crystallinity and crystal size decreased proportionally to pyrolysis temperature. The iso-conversional method coupled with the Kissinger and Flynn-Wall-Ozawa methods were used to determine kinetic parameters via differential scanning calorimetry analysis with different heating rates. The active energy of the reaction was 171.1 and 169.1 kJ/mol, calculated with the two methods respectively and implied the sensitivity of the reaction with temperature.

• Carbon letters
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• 제10권4호
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• pp.325-328
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• 2009

This cycloaddition of [70]fullerene with methyl azidoacetate in benzene under ultrasonic irradiated condition afforded the closed [5,6]-bridged aziridino[70]fullerene derivative, which was unusual product of cycloaddition to the 5,6-junction of fullerene. Its structure was determined by FAB-MS, UV-vis, $^1H-$ and $^{13}C$-NMR spectral data. The closed [5,6]-bridged aziridino[70]fullerene-functionalized gold nanoparticle films were self-assembled using the layer-by-layer method on the reactive of glass slides functionalized with 3-mercaptopropyl trimethoxysilane. The functionalized glass slides were alternately soaked in the solution containing closed the [5,6]-bridged aziridino[70]fullerene and 4-aminothiophenoxide/hexanethiolate-protected gold nanoparticles. The closed [5,6]-bridged aziridino[70]fullerene-functionalized gold nanoparticle films have grown up to 5 layers depending on the immersion time. The self-assembled nanoparticle multilayer films were characterized using UV-vis spectroscopy showed that the surface plasmon band of gold at 527 nm gradually became more evident as successive layers were added to the films.

• KSBB Journal
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• 제16권4호
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• pp.409-414
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• 2001

본 연구에서는 Alcaligenes faecalis에 의해 생산되는 세포 외 다당류인 $\beta$-glucan의 생합성에서 탄소원, 질소원의 영향과 생합성된 curdlan의 구조를 살펴보았다. 먼저 $\beta$-glucan의 생합성을 확인하기 위해 Aniline Blue 배지에서 청색 colony들을 선별하고 Cellufluor 배지에서 형광성을 보임으로 $\beta$-1,3 glucan의 생합성을 확인하였다. Flask 배양에서는 탄소원이 glucose이고 질소원이 ($NH_4$)$_2$$SO_4$일 때 가장 많은 $\beta$-glucan을 생산하였다. 탄소원을 glucose로 질소원을 (NH$_4$)$_2$$SO_4$로 사용한 발효조 배양에서는 flask 배양에 비해 생산량이 7.2%가 증가되었다. 질소원을 제한한 발효에서는 최종 생산수율이 38.82%였고 질소원을 제한하지 않은 발효보다 3.02%가 증가 되었다. 분리 정제된 curdlan의 FT-IR의 분석에서는 지문영역인 890$cm^{-1}$ /에서 $\beta$-form이 관찰되었고 $^{13}C$-NMR 분석에서는 $C_1$-103.5 ppm, $C_2$-74.3 ppm, $C_3$-89.8 ppm, $C_4$-68.8 ppm, $^{5}C$-75.5 ppm, $C_2$-61.8 ppm에서 검출되어 homogeneous $\beta$-1,3 glucan으로 확인되었다. curdlan의 triple-helix coil 형태에서 random coil 형태로의 전이는 0.1에서 0.25 N NaOH의 농도에서 일어남이 확인되어 중성 상태의 curdlan은 triple-helix coil 형태를 가지지만 알칼리 상태에서는 그 구조가 약해져 random-coil로 전이 되어짐을 알 수 있었다. 이는 본 연구에서 분리 정제된 curdlan이 정확한 $\beta$-결합을 가진 glucan임이 확인되었으며 생합성된 curdlan의 구조와 분자적인 특성을 확인하였다. 본 연구를 통하여 $\beta$-결합을 가지는 glucan의 신속한 선별법을 구축할 수 있었고 이는 새로운 기능을 가지는 $\beta$-glucan 생합성 효소나 분해효소의 탐색에 이용될 것으로 기대된다.

• 펄프종이기술
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• 제47권4호
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• pp.144-150
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• 2015

Stems of Pinus thunbergii Parl. grown with high salinity were analyzed for chemical characteristics. Stem of 2 years was rich in soluble compounds and stem of 3 years reduced the amount of the soluble compound. But, the lignin content have not seen a large change. Also, Klason lignins of stem of 2 and 3 years has not changed in nitrogen and hydrogen content. In Klason process, it was significantly increased the carbon concentration due to the hydrolysis of the carbohydrate. In addition, the accumulation of xylan from Pinus thunbergii Parl. with salinity treatment were increased noticeably. Finally, functional group of Pinus thunbergii Parl. with salinity treatment were not changed.