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Isolation and Purification of an Antitumor Metabolite from Alternaria brassicicola SW-3, the Cause of Brassica Black Leaf Spot Disease.  

나여정 (서울여자대학교 식품ㆍ미생물공학과)
이방숙 (서울여자대학교 화학과)
남궁성건 (서울여자대학교 화학과)
정동선 (서울여자대학교 식품ㆍ미생물공학과)
Publication Information
Microbiology and Biotechnology Letters / v.30, no.1, 2002 , pp. 51-56 More about this Journal
Abstract
An antitumor substance was purified from the culture filtrate of phytopathogenic fungus Alternaria brassicicola SW-3 isolated from soil of a chinese cabbage patch, and its characteristics were investigated. Antitumor activity of A. brassicicola SW-3 was measured by MTT assay. The cytotoxic activity against human cancer cell line was detected in the culture filtrate of A. brassicicola SW-3, but no activity found in mycelium. Antitumor substance was isolated from the culture broth by ethyl acetate extraction and purified by silica gel column chromatography. Structure of the purified compound was analyzed by the instrumental analysis such as $^1$H-NMR, $^{13}$ C-NMR and IR spectroscopy. The purified fungal metabolite of an A. brassicicola SW-3, consists of 11 carbon chain with two hydroxyl groups and two epoxides which is identical to depudecin. The $IC_{50}$/ values of the active compound identified as depudecin were $69\mu$g/mL and $57\mu$g/mL against mouse melanoma B16BL6 cell line, and human hepatoma SK-HEP1 cell line, respectively.
Keywords
Alternaria brassicicola; Fungal metabolites; Antitumor activity; Depudecin;
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