• 생명과학회지
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• 제13권3호
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• pp.324-332
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• 2003

최근 Phenobarbital에 의해서 발현되는 CYP2B유전자의 발현촉진 매개인자로서 Constitutive Androstane Receptor (CAR)가 최근에 규명되었다. 사람의 간암세포인 HepG2 cell line에 CAR유전자를 transfection시켰을 경우 PBRU의 활성을 촉진시켰다. 지금까지 연구된 CAR의 역할은 주로 간세포 내에서 cytochrome P4502B 유전자의 발현을 촉진하는 Phenobarbital의 매개체로서 알려져 있다. 다세포동물에서 각각의 분화된 세포는 독특한 유전자의 발현 Pattern을 갖고 있어서 어느 특정한 세포에서 일어나는 유전자의 발현특징이나 작용기전 혹은 기능이 다른 종류의 세포에서는 일어나지 않거나 상이한 기전에 의해서 조절된다. 이러한 세포간 특이성을 이용하여 유전자 발현에 관여하는 인자들을 규명하는데 기초적 자료로 이용될 수 있다. 따라서 본 연구의 목적은 CYP2B유전자의 발현이 일어나지 않는 콩팥세포 line (COS)에서 CAR 단백질 수용체가 PBRU의 활성촉진에 영향을 미치는지의 여부를 조사하고자 하였다. Control 상태의 Hep G2와 COS 배양세포에서는 CAR 단백질의 발현이 거의 나타나지 않았다. mCAR1-GFP를 transfection 한 후 CAR antibody를 이용하여 immunoblot을 시행하였을 경우, Hep G2 세포에서는 발현이 비교적 약하게 나타났지만, COS 세포에서는 강한 mCAR1-GFP 단백질의 발현을 보였다. 한편, GFP antibody를 이용하여 immunoblot을 시행하였을 경우에는 COS 세포에서 강한 mCAR1-GFP 단백질의 발현을 나타내었을 뿐만 아니라 Hep G2 세포에서도 명백히 단백질의 발현을 관찰할 수 있었다. 또한, Hep G2와 COS세포 모두에서 endogenous RXR의 발현이 일어남을 확인하였고 RXR expression plasmid를 transfection시켰을 때 두 세포 모두에서 단백질의 발현이 현저하게 증가되었다. Constitutive Androstane Receptor (CAR)에 의한 CYP2B의 PBRU 활성효과를 다르게 분화된 세포에서 차이가 일어나는지를 비교하기 위하여 CAR에 의해 매개되는 PBRU의 transactivation효과를 Hep G2와 COS세포에서 조사하였다. Hep G2 세포에서는 transfection된 CAR의 발현에 의해 firefly luciferase 보고단백질의 활성이 약 12배 증가하였다. CAR 발현유전자를 15 ng transfection하였을 때 주어진 보고유전자의 양에 대하여 최대반응을 나타내었고 CYP2B1PBRU가 제거된 CYP2C1 promotor/firefly luciferase를 보고유전자로 사용하였을 때는 CAR에 의한 luciferase의 활성이 나타나지 않았다. Hep G2와는 달리, COS세포에서는 transfection된 CAR의 발현이 PBRU에 의한 firefly luciferase보고단백질의 발현에 영향을 주지 못하였다. 이러한 결과들은 분화된 세포의 종류에 따라서 constitutive androstane receptor의 CYP2BPBRU 활성효과가 다르게 나타날 수 있음을 제시할 뿐만 아니라, 간세포에서 Phenobarbital에 의한 PBRU의 활성유도에 영향을 주는 endogenous 매개 인자들 중 CAR와 RXR과는 다른 전사조절인자들이 필요로 하며 이러한 인자들의 발현이 콩팥 세포에서는 다르게 존재함을 시사한다.

• 대한음성학회지:말소리
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• 제48호
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• pp.141-153
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• 2003

Researches into robust recognition in noise environments, especially in car environments, are being carried out actively in speech community. In this paper we will report on three types of corpora that SiTEC (Speech Information TEchnology & industry promotion Center) has created for research into speech recognition in car noise environments. The first is the recordings of 900 Korean native speakers, distributed according to gender, age, and region, who uttered application words in car environments. The second is the collections of mixed noise in 3 car types by model while setting up various noise patterns which can be obtained with the car engine on or off, at different driving speed, and in different road conditions with windows open or closed. The third is the recordings of simulated speech by HATS (Head and Torso Simulator) in car environments with the internal and external noise factors added. These three types of recordings were all made through synchronized 8 channel microphones that are fixed in a car. The creation and applications of these corpora will be reported on in detail.

• 한국정보통신학회논문지
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• 제6권2호
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• pp.339-345
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• 2002

자동차의 번호판을 인식하는 것은 차량을 식별하는데 있어서 매우 중요하다. 어두운 조명에서나 날씨가 나쁠 경우 차량의 형상이 왜곡 될 수 있고, 번호판을 식별하는데 어려움이 있다. 본 논문은 차량의 규격을 이용하여 효율적으로 번호판을 추출하는 방법을 제안한다. 이 방법에서 색상이나 형태처럼 차량의 규격을 따르는 자동차 번호판의 특징들은 번호판의 후보영역으로 결정되고, 신경망에 의해 숫자나 문자의 패턴 갖는 영역이 번호판 영역으로 인식된다. 또한 특징패턴인식의 결과로서 번호판을 확정하였다. 70개 차량영상을 실험해 본 결과 번호판 추출률에서는 84.29 ％, 인식률에서는 80.81 ％의 결과를 나타내었다.

• 한국기계가공학회지
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• 제19권1호
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• pp.56-62
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• 2020

The purpose of this study is to observe the visualization of the flow field for air flow distributed in the car from the ventilation fan installed in the ceiling of the passenger elevator car through the numerical analysis using computational fluid dynamics. STAR-CCM+, which is a code used for the numerical analysis, was used to predict the airflow distribution inside the elevator car. The numerical analysis of the distribution of the air current in the elevator was carried out. As a result, the analysis results for each point and the visualization of the air current distribution and the temperature distribution in the elevator car and were obtained. It was found that heat transfer was actively occurring inside the car due to the influence of the flow field discharged from the ventilation vent installed in the ceiling in the elevator car, and especially the convection heat transfer of Model-2 was more active than that of Model-1. As a result, the temperature distribution inside the car was found to be relatively low. In addition, the temperature distribution at a cross-section of 1700mm height in the elevator car shows that Model-2 is the location of the ventilation vent which makes people feel more comfortable.

• International Journal of Automotive Technology
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• 제8권1호
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• pp.67-73
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• 2007

This study analyzes the interior noise that is generated during acceleration of a passenger car in terms of car body structure and panel contribution. According to the transfer method, interior noise is classified into structure-borne noise and air-borne noise. Structure-borne noise is generated when the engine's vibration energy, an excitation source, is transferred to the car body through the engine mount and the driving system and the panel of the car body vibrates. When structure-borne noise resonates in the acoustic cavity of the car interior, acute booming noise is generated. This study describes plans for improving the car body structure and the panel form through a cause analysis of frequency ranges where the sound pressure level of the rear seat relative to the front seat is high. To this end, an analysis of the correlation between body attachment stiffness and acoustic sensitivity as well as a panel sensitive component analysis were conducted through a structural sound field coupled analysis. Through this study, via research on improving the car body structure in terms of reducing rear seat noise, stable performance improvement and light weight design before the proto-car stage can be realized. Reduction of the development period and test car stage is also anticipated.

• 한국생산제조학회지
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• 제20권3호
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• pp.280-283
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• 2011

This study investigates the durability of car body and the safety of passenger inside car body in the case of the impact contact at passenger and car body. In case of front impact contact, maximum von Mises equivalent stress and principal stress become 3240.7MPa and 1634MPa respectively at the rear part of car body and the neck of dummy. And maximum total deformation occurred with 14.145mm at the hand of dummy. In case of side impact contact, maximum von Mises equivalent stress and principal stress become 7687.9MPa and 1690.7MPa respectively at the front part of car body and the lap of dummy. And maximum total deformation occurred with 16.414 mm at the foot of dummy. In case of rear impact contact, maximum von Mises equivalent stress and principal stress become 2366.6MPa and 1447MPa respectively at the front part of car body and the neck of dummy. And maximum total deformation occurred with 7.548mm at the rear part of car body. As the maximum von-Mises stress at side impact is shown with more than 700MPa as over two times at front or rear impact the danger of car body is increased. The great possibility of damage is shown at neck and hand of dummy with more than total displacement of 10mm.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2007년도 심포지엄 논문집 정보 및 제어부문
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• pp.369-372
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• 2007

about commercial business of car black box, mirror vision for car black box deal with analysis of Existing Car Black boxes in market to provide the objective information associated with surrounding scene of car instead of witness, we experimented on suitable structure of all direction to cover surrounding of car considering dead zone where can't see at short distance and realized simple structure of gathering scene using mirror and lens and by saving the number of camera and MUX of pre-circut

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2000년도 제15차 학술회의논문집
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• pp.217-217
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• 2000

A decentralized robust EA(eigensoucture assignment) controller is designed for an active suspension system of a vehicle based on a full car model with 7-degree of freedom. Using overlapping decomposition, the full car model is decentralized by two half car models. For each half car model, a robust eigenstructure assignment controller can be obtained by using optimization approach. The performance of the decentralized robust EA controller is compared with that of a conventional centralized EA controller through computer simulations.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2008년도 춘계학술대회 논문집
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• pp.1073-1078
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• 2008

This paper is mainly concerned with the characteristics of precision stop on the automatic operating for the train car. Recently, the automatic operation of train car and the PSD establishment inside the subway are magnified. Consequently, the importance and necessity of characteristic of train car precision position stop are augmented. It researches the characteristic of precision position stop when the train car is operated with automatically and plans the safe operation of train car and the method of maintenance which is rational.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2003년도 추계학술대회 논문집(III)
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• pp.109-113
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• 2003

Recently, freight cars for diverse purposes were developed to increase the speed of freight car in conventional line. The welded type bogies are applied to these vehicles like container car, gondola car, hopper car, etc. This study was focused on the dynamic characteristics and the stability of freight car to guarantee the running safety. We have conducted the running tests of freight cars in conventional line and described the test results in this paper.