• Bulletin of the Korean Chemical Society
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• 제34권5호
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• pp.1401-1406
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• 2013

This study tested the feasibility of observing H/D exchange of intact protein by top-down electron capture dissociation (ECD) mass spectrometry for the investigation of protein structure. Ubiquitin is selected as a model system. Local structural information was obtained from the deuteration levels of c and $z^{\cdot}$ ions generated from ECD. Our results showed that ${\alpha}$-helix region has the lowest deuteration level and the C-terminal fraction containing a highly mobile tail has the highest deuteration level, which correlates well with previous X-Ray and HDX/NMR analyses. We studied site-specific H/D exchange kinetics by monitoring H/D exchange rate of several structural motives of ubiquitin. Two hydrogen bonded ${\beta}$-strands showed similar HDX rates. However, the outer ${\beta}$-strand always has higher deuteration level than the inner ${\beta}$-strand. The HDX rate of the turn structure (residues 8-11) is lower than that of ${\beta}$-strands (residues 1-7 and residues 12-17) it connects. Although isotopic distribution gets broader after H/D exchange which results in a limited number of backbone cleavage sites detected, our results demonstrate that this method can provide valuable detailed structural information of proteins. This approach should also be suitable for the structural investigation of other unknown proteins, protein conformational changes, as well as protein-protein interactions and dynamics.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제13권12호
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• pp.5948-5971
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• 2019

Estimating loss rates with the packet traces captured from some point in the middle of the network has received much attention within the research community. Meanwhile, existing intermediate-point methods like [1] require the capturing system to capture all the TCP traffic that crosses the border of an access network (typically Gigabit network) destined to or coming from the Internet. However, limited to the performance of current hardware and software, capturing network traffic in a Gigabit environment is still a challenging task. The uncaptured packets will affect the total number of captured packets and the estimated number of packet losses, which eventually affects the accuracy of the estimated loss rate. Therefore, to obtain more accurate loss rate, a method of strengthening packet loss measurement from the network intermediate point is proposed in this paper. Through constructing a series of heuristic rules and leveraging the binomial distribution principle, the proposed method realizes the compensation for the estimated loss rate. Also, experiment results show that although there is no increase in the proportion of accurate estimates, the compensation makes the majority of estimates closer to the accurate ones.

• Carbon letters
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• 제2권2호
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• pp.113-119
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• 2001

The advanced method for $CO_2$capture is currently one of the most important environmental issues in worldwide and it is therefore necessary to have available technologies, which minimize the discharge of $CO_2$ including Carbon-14 from nuclear facilities into the atmosphere. A key aspect of this work is to provide the technically principal data required to improve a $CO_2$ removal system for the utilization of regenerative sorbent use, specifically include suggestions regarding its modified column design (parallel dual-bed assembly), stop-restart operation and the economic feasibility of sorbent use. The removal performance of soda lime and the effects of relative humidity (RH) and packing bed-depth (BD) on $CO_2$ removal were investigated. In a single-bed, it revealed that the utilization of soda lime for $CO_2$ removal at line velocity of 13 cm/sec and bed depth of 12 cm increased with the increased relative humidity up to 85%. However, in the parallel dual-bed assembly applied with the stop-restart operation, a maximum utilization rate of soda lime for $CO_2$ removal was obtained even at 55% of RH and 8 cm of BD, specifically the utilization rate of soda lime by using this $CO_2$ removal assembly was about two-fold superior to that in a single-bed.

• 한국수소및신에너지학회논문집
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• 제24권5호
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• pp.428-436
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• 2013

Attrition characteristics of $CO_2$ absorbents for pre-combustion $CO_2$ capture were investigated to check attrition loss of those absorbents and to determine solid circulation direction and the better $CO_2$ absorbent. The cumulative attrition losses of two absorbents increased with increasing time. However, attrition loss under a humidified condition was lower than that under a non-humidified condition case. Between two absorbents, attrition loss of PKM1-SU absorbent was higher than that of P4-600 absorbent. The average particle sizes of the attrited particles were less than $2.5{\mu}m$ for two absorbents under a non-humidified condition case, and therefore, we could conclude that the main mechanism of attrition for two absorbents is not fragmentation but abrasion. Based on the results from the test for the effect of humidity on the attrition loss, we selected solid circulation direction from SEWGS reactor to regeneration reactor because the SEWGS reactor contains more water vapor than regeneration reactor. Attrition loss and make-up rate of two absorbents were compared based on the results from $CO_2$ sorption capacity tests and attrition tests. Required make-up rate of P4-600 absorbent was lower than that of PKM1-SU absorbent. However, more detail investigation on the optimum regeneration temperature, manufacturing cost, solid circulation rate, regeneration rate, and long-term sorption capacity should be considered to select the best $CO_2$ absorbent.

• 터널과지하공간
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• 제26권1호
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• pp.12-23
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• 2016

향후 국내에서 수행될 이산화탄소 지중저장 프로젝트의 성공적인 수행을 위해서는 현장 지질조건에 최적화된 주입 조건을 설계하는 것이 중요하다. 따라서 본 연구에서는 주입량 및 주입온도 등의 주입조건이 격리저장시스템의 역학적 안정성에 미치는 영향을 열-수리-역학 연계해석기법의 하나인 TOUGH-FLAC 해석기법을 이용하여 평가하였다. 저장시스템의 역학적 안정성은 기존 균열의 전단미끄러짐 발생가능성을 활동마찰각 및 응력원을 이용하여 분석하였다. 이산화탄소의 주입온도가 저류층의 초기온도보다 낮은 저온주입의 조건에도 열응력으로 인한 인장균열은 발생하지 않는 것으로 파악되었으나, 저류층에서 전단미끄러짐이 발생하는 결과를 보였다. 단위시간당 이산화탄소 주입량을 변화시킨 시나리오 해석에서는 단계별로 주입량을 감소시키는 주입시나리오에서 기존 균열들의 전단미끄러짐 발생 가능성이 가장 낮은 것으로 평가되었다.

• Fisheries and Aquatic Sciences
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• 제15권4호
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• pp.337-343
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• 2012

The Korean bottom trawl survey was conducted using a trawl designed by the National Fisheries Research and Development Institute (NFRDI). The capture efficiency and size selectivity of this trawl for snow crab Chionoecetes opilio was investigated by experimental tows. An auxiliary sampling net (underbag) was attached beneath the trawl net to capture crabs escaping under the trawl footrope. Experimental tows were made by the same vessel speed (3.4 knots) as in the bottom trawl survey, but toing time was shortened from the standard 30 min to 10 min to reduce possible trawl distortion due to the high catch rate of mud and debris in the underbag. In averaged over 17 tows conducted between 110-383 m depth, trawl efficiency of both males and females combined increased from about 10% at 20 mm (carapace width) to about 70% at 100 mm, with a width of 50% capture equal to 78 mm.

• 한국물환경학회지
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• 제29권3호
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• pp.354-364
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• 2013

The performance of the stormwater wetlands can be significantly influenced by antecedent stormwater in storage at the commencement of a stormevent. As inflows are intermittent and stochastic in nature, the evaluation of the treatment efficiency of a stormwater wetland should be considered by runoff capture and water treatment characteristics during interevent periods. In this study, analytical probabilistic model is applied to identity runoff capture rate and treatment efficiency of the stormwater wetland. To achieve this, continuous rainfall data recorded in Busan for 31 years has been analyzed to derive the runoff capture rate, and 1st order kinetic decay constants ($k_V$, 1/d) are calculated from regression analysis to identify pollutants removal during interevent periods. The results show that about 60.9% of annual average runoff is captured through the stormwater wetland. The annual average treatment efficiencies of SS, BOD, COD, TN and TP is about 11.4, 8.9, 9.8, 4.3 and 9.6%, respectively. The analytical model has been compared with the numerical model and it shows that analytical model is valid. Performance evaluation methods developed in this study has the advantages of considering characteristics of rainfall-runoff, facility type and pollutant removal.

• 한국콘텐츠학회논문지
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• 제13권8호
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• pp.308-313
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• 2013

세포선별검사에서 ASCUS로 진단된 검체 중 F-UP이 진행되지 않은 검체 179건을 대상으로 HPV high risk factor, low risk factor를 확인하기 위해서 Hybrid Capture II Test를 하였다. 시험 결과 high risk factor 양성 및 low risk factor 양성은 전체 179 cases 중 26 cases로 나타났으며, 전체 검체 중 약 15%에 해당되었다. 또한 전체 15%의 양성검체 중 high risk factor 양성은 96%에 해당되는 것을 확인하였다. 결론적으로 효과적인 검진 체계를 갖춘 나라에서도 자궁경부암의 예방이 완전히 해결되지 않고 있는 문제점이 있는 여건을 고려해 볼 때 추적 관찰이 용이하지 않은 경우를 대비해 자궁경부암으로 진행될 수 있는 precancerous lesion의 원인 인자인 HPV 존재 유무를 세포선별검사와 HPV Hybrid Capture II Test를 동시에 시행하는 것이 효과적이라고 사료된다.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2021년도 춘계학술대회
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• pp.5-7
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• 2021

자세 추정을 위한 모션 캡처 데이터 파일에는 주변 환경과 움직임의 정도에 따라 부정확한 데이터가 존재할 수 있으므로, 이를 보정하는 작업이 필요하다. 기존에는 직접 후처리 과정을 통해 부정확한 데이터를 복원하였으나, 최근에는 자동화된 방법으로 LSTM, R-CNN 등 다양한 종류의 신경망을 사용한다. 하지만 신경망 기반의 데이터 복원 방법들은 컴퓨터 자원을 많이 요구하므로, 본 논문에서는 신경망 기반의 방법보다 자원 사용량은 낮추면서 데이터 복원율은 유지하는 방법을 제안한다. 제안하는 방법은 자세 측정 데이터(c3d)를 활용하여 부정확한 자세 데이터를 자동으로 복원한다. 실험 결과, 데이터의 부정확한 정도에 따라 89%에서부터 99% 정도의 데이터 복원율을 보였다.

• 국제학술발표논문집
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• The 8th International Conference on Construction Engineering and Project Management
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• pp.257-264
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• 2020

Objective: To investigate test-retest reliability and responsiveness of Equivital Lifemonitor and photoplethysmography based wristwatch tools in assessing physiological parameters during a simulated fatigue task. Methods: Ten university students (Mean age, 30.6 ± 1.7 years) participated in this pilot study. Participants were asked to perform a 30-minute of a simulated fatigue task in an experimental setup in a lab. The physiological parameters (e.g., heart rate, heart rate variability, respiratory rate, electrodermal activity, and skin temperature) were measured at baseline and immediately after the fatigue task. An intraclass correlation coefficient (ICC2,1) was used to evaluate the test-retest reliability of each tool in assessing physiological measures. In addition, the responsiveness of each tool to measure changes from baseline to posttest was calculated using a standardized response mean. Results: The Equivital Lifemonitor has shown good to excellent test-retest reliability for the assessment of heart rate (ICC, 0.97), heart rate variability (ICC, 0.86), respiratory rate (ICC, 0.77), and local skin temperature (ICC, 0.76). However, photoplethysmography based wristwatch showed moderate to good test-retest reliability for the assessment of heart rate (ICC, 0.71), heart rate variability (ICC, 0.73), electrodermal activity (ICC, 0.80), and skin temperature (ICC, 0.72). A large standardized response mean (>0.8) indicates that both tools can capture the changes in heart rate, heart rate variability, respiratory rate, skin temperature, and electrodermal activity after a 30-minute of fatigue task. Conclusions: The Equivital Lifemonitor and photoplethysmography based wristwatch devices are reliable in measuring physiological parameters after the fatigue task. Additionally, both devices can capture the fatigue response after a simulated construction task. Future field studies with a larger sample should investigate the sensitivity and validity of these tools in measuring physiological parameters for fatigue assessment at construction sites.