• The Plant Pathology Journal
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• 제20권1호
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• pp.46-51
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• 2004

Host resistance is usually parasite-specific and is restricted to a particular pathogen races, and commonly is expressed against specific pathogen genotypes. In contrast, resistance shown by an entire plant species to a species of pathogen is known as non-host resistance. Therefore, non-host resistance is the more common and broad form of disease resistance exhibited by plants. As a first step to understand the mechanism of non-host plant defense, expressed sequence tags (EST) were generated from a hot pepper leaf cDNA library constructed from combined leaves collected at different time points after inoculation with non-host soybean pustule pathogen (Xanthomonas axonopodis pv. Glycines; Xag). To increase gene diversity, ESTs were also generated from cDNA libraries constructed from anthers and flower buds. Among a total of 10,061 ESTs, 8,525 were of sufficient quality to analyze further. Clustering analysis revealed that 55 ％ of all ESTs (4685) occurred only once. BLASTX analysis revealed that 74％ of the ESTs had significant sequence similarity to known proteins present in the NCBI nr database. In addition, 1,265 ESTs were tentatively identified as being full-length cDNAs. Functional classification of the ESTs derived from pathogen-infected pepper leaves revealed that about 25％ were disease- or defense-related genes. Furthermore, 323 (7％) ESTs were tentatively identified as being unique to hot pepper. This study represents the first analysis of sequence data from the hot pepper plant species. Although we focused on genes related to the plant defense response, our data will be useful for future comparative studies.

• 농업생명과학연구
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• 제43권5호
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• pp.1-8
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• 2009

고추 12 품종의 SMP 처리시 carrier와 수분의 배율, 처리기간 및 처리온도에 대한 조사 결과 적정 처리기간과 carrier와 수분의 배율은 품종마다 차이는 있었지만, 처리 중 발아가 일어나지 않았고, 발아율을 높이며, $T_{50}$을 단축시킬 수 있는 처리로 왕과 마니따 품종은 carrier와 수분의 배율이 1:3에서 7일이었다. 나머지 10 품종은 1:7의 비율에서 3일이 좋았다. 종가집 품종은 다른 품종에 비해 처리기간과 수분배율에 따라 발아율과 $T_{50}$이 불규칙한 경향을 나타낸 품종으로 조사되었다. 처리온도는 $15^{\circ}C$에서 $35^{\circ}C$로 설정하여 조사한 결과, 처리 중 유근돌출의 억제와 발아성 향상에 안정적인 처리는 $20^{\circ}C$인 것으로 조사되었다.

• 한국식품영양과학회지
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• 제46권11호
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• pp.1350-1357
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• 2017

본 연구는 오이의 종류와 조리방법에 따라 일반성분 및 무기질 함량을 비교하고자 하였다. 오이는 취청오이, 백다다기오이, 노각 3종류를 생재료와 삶기, 찌기, 굽기, 로스팅, 볶기, 튀기기, 전자레인지 7가지 조리방법을 사용하였다. 이를 통해 일반성분과 무기성분의 잔존율과 각각의 함량에 대한 종류 및 조리방법의 상관성을 조사하였다. 실험에 사용된 오이는 모두 조리과정에서 중량이 감소하였다. 대부분의 일반성분과 무기질은 종류와 조리방법의 영향을 모두 받았지만 단백질은 영향을 받지 않았고, 철 함량은 조리방법에 의해 영향을 받지 않았으며, 구리 함량은 종류에 대한 영향을 받지 않는 것으로 나타났다. 일반성분 중 단백질, 지방, 회분은 튀기기 했을 때 함량이 높았고 수분은 원재료, 삶기, 찌기를 했을 때 높았다. 무기질은 로스팅과 전자레인지 조리를 한취청오이의 함량이 높았고, 백다다기오이와 노각은 튀기기를 했을 때 전반적인 함량이 높았다. 단백질, 지방, 수분, 회분의 잔존율은 각각 30~100%, 10~100%, 35~90%, 30~100% 범위로 나타났다. 무기질은 로스팅, 전자레인지, 삶기를 한 취청오이가 높은 잔존율을 보였고, 백다다기오이와 노각은 튀기기를 했을 때 높은 잔존율을 보였다. 취청오이는 찌기를 했을 때, 백다다기오이와 노각은 삶기와 찌기를 했을 때 잔존율이 낮았으며, 특히 노각의 경우 20~45%로 낮은 잔존율을 보였다. 이러한 결과들을 종합해 보았을 때 오이에 대한 조리 전후에 대한 기초 자료로 활용이 가능할 것으로 생각되며, 다양한 오이 종류에 대한 추가적인 연구가 필요할 것으로 생각된다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.149.2-150
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• 2003

Occurrences of virus diseases on paprika ( Capsicum annuum var. grossum) were surveyed in Joennam province from 1999 to 2003 and the collected samples showing virus-like symptoms were tested using ELISA. Virus diseases appeared 4.5％, 17.5％, and 4.9％ in 2000, 2002, and 2003, respectively. As the results of investigation of the seasonal incidence with the growing stages of plant, virus was not occurred at seedling stage and was slightly from the planting time to the first harvesting time, but was dramatically increased at the second harvesting time. Virus diseases were more severe on the vinyl house than on the green house. Pepper mild mottle virus (PMMoV) was severely occurred in 2000 but not after that year. Comparing the virus species, Pepper mottle virus (PepMoV) was 35.9％, Broad bean wilt virus (BBWV) was 14.1％, and Cucumber mosaic virus (CMV) was 10.9％ in 2002, and 76.0％, 11.1％, and 2.4％ in 2003, respectively.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2010년도 정기총회 및 춘계학술발표회
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• pp.6-6
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• 2010

Bacteria of the Bacillus sp. are well known to possess antagonistic activity against numerous plant pathogens. In the present study, 11 Bacillus sp. were isolated from the brackish environment and assayed for antagonistic activity under in vitro and in vivo conditions. Among the 11 isolates tested, 9 isolates effectively inhibited the growth of various plant pathogens, namely Phytophthora capsici, Phytophthora citropthora, Phytophthora citricola, Phytophthora sojae, Colletotricum coccodes, Colletotricum gloeosporioides, Colletotricum acutatum, Rhizoctonia solani, Fusarium solani, Fusarium graminearum, Pyricularia sp. and Monilina sp. The effective isolates were further screened for Phytophthora blight suppression in Capsicum annuum L. under green house conditions. The isolate SB10 exhibited the maximum (72.2%) reduction in disease severity. The antifungal compounds from the isolate were isolated and characterized. The isolated compounds exhibited high thermo stability ($100^{\circ}C$ for 30 min). Matrix-Assisted Laser Desorption Ionization-Time of Flight investigation of the antifungal compounds revealed three lipopeptide complexes, the surfactins, the iturins, and the fengycins.

• The Plant Pathology Journal
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• 제20권2호
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• pp.85-91
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• 2004

A disease causing severe leaf spots in pepper plants has been observed in northern Gyeongbuk and Gangwon provinces in Korea since 1994. The current study diagnosed the disease as gray leaf spot caused by Stemphylium solani Weber and S. lycopersici (Enjoji) Yamamoto, both of which are pathogenic in pepper and tomato plants. Although the disease has been found in almost all areas where peppers are grown, it is more severe in mountain terrains where the nights are cool. Both species of pathogenic fungi were found to sporu-late profusely on V-8 juice agar in plastic or Pyrex glass Petri dishes, although not in domestically-produced glass Petri dishes, when cultured at $20^{\circ}C$ under irradi-ation from a daylight fluorescent lamp with a 12-hour light and dark alternation. The domestically-produced glass Petri dishes, which are made of window glass, were found to block near ultraviolet wavelengths, around and below 300 nm, which explained why the fungi did not sporulate. However, sporulation decreased at above $25^{\circ}C$ and most isolates failed to sporulate above $27^{\circ}C$. The worst level of disease was obtained when the inoculated plants were incubated with a $15^{\circ}C$ night and $20^{\circ}C$ day temperature regime relative to 4 night/day temperature combinations (15/20, 20/25, 25/30, and 30/35$^{\circ}C$).

• The Plant Pathology Journal
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• 제29권3호
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• pp.285-293
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• 2013

Leaf samples of Solanum lycopersicum, Capsicum annuum, Cucurbita moschata, Cucurbita pepo, Sechium edule and Erythrina spp. were collected. All samples were positive for begomoviruses using polymerase chain reaction and degenerate primers. A sequence of ~1,100 bp was obtained from the genomic component DNA-A of 14 samples. In addition, one sequence of ~580 bp corresponding to the coat protein (AV1) was obtained from a chayote (S. edule) leaf sample. The presence of Squash yellow mild mottle virus (SYMMoV) and Pepper golden mosaic virus (PepGMV) were confirmed. The host range reported for SYMMoV includes species of the Cucurbitaceae, Caricaceae and Fabaceae families. This report extends the host range of SYMMoV to include the Solanaceae family, and extends the host range of PepGMV to include C. moschata, C. pepo and the Fabaceae Erythrina spp. This is the first report of a begomovirus (PepGMV) infecting chayote in the Western Hemisphere.

• 한국균학회지
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• 제23권2호통권73호
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• pp.114-120
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• 1995

병든 고추에서 수집한 종자에서 Colletotrichum dematium, C. gloeosporioides 및 Glomerella cingulata 등이 습지법에 의해서 검출되었다. C. gloeosporioides는 공시된 종자시료에서 우점종이었으며 그 다음이 C. dematium, G. cingulata의 순이었다. 탄저병균의 종자내 감염부위를 조사한 결과, 종피, 배유 및 자엽에서 C. dematium, C. gloeosporioides 및 G. cingulata가 검출되었으며 3종의 탄저병균의 검출율은 종피에서 더 많았고 배유와 자엽에서는 적었다. C. dematium, C. gloeosporioides와 G. cingulata의 종자감염은 종자부패, 모잘록 및 모마름병을 일으켰다. 접종시험결과 C. gloeosporioides가 가장 병원성이 강하였다. C. dematium은 상처가 있을 때 약한 병원성을 나타냈으며 G. cingulata는 유상 또는 성숙과에 대해서 약한 병원성이 있었다. 탄저병에 대한 종자소독 결과 베노람 수화제와 지오람 수화제의 효과가 우수하였다.

• Molecules and Cells
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• 제26권3호
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• pp.250-257
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• 2008

Microsatellites or simple sequence repeats (SSR) are widely distributed in eukaryotic genomes and are informative genetic markers. Despite many advantages of SSR markers such as a high degree of allelic polymorphisms, co-dominant inheritance, multi-allelism, and genome-wide coverage in various plant species, they also have shortcomings such as low polymorphic rates between genetically close lines, especially in Capsicum annuum. We developed an alternative technique to SSR by normalizing and alternating anchored primers in random amplified microsatellite polymorphisms (RAMP). This technique, designated reverse random amplified microsatellite polymorphism (rRAMP), allows the detection of nucleotide variation in the 3' region flanking an SSR using normalized anchored and random primer combinations. The reproducibility and frequency of polymorphic loci in rRAMP was vigorously enhanced by translocation of the 5' anchor of repeat sequences to the 3' end position and selective use of moderate arbitrary primers. In our study, the PCR banding pattern of rRAMP was highly dependent on the frequency of repeat motifs and primer combinations with random primers. Linkage analysis showed that rRAMP markers were well scattered on an intra-specific pepper map. Based on these results, we suggest that this technique is useful for studying genetic diversity, molecular fingerprinting, and rapidly constructing molecular maps for diverse plant species.

• 한국토양비료학회지
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• 제32권1호
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• pp.68-75
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• 1999

인산축적 시설재배지에서 Arbuscular mycorrhizal fungj의 이용을 위한 기초자료를 얻고자 비교적 내생균근균의 의존도가 높은 고추를 대상으로한 AMF의 전종방법 및 인간시용량에 따른 AMF의 접종효과를 검토하였다. 두 균주의 접종효율을 증진하기 위하여 접종시기, 접종밀도 및 배합토내 인산수준에 따른 고추의 생장반응을 본 결과, 배지내 인산수준별로는 $200mg\;P\;kg.soil^{-1}$이하의 수준에서 AMF접종시 유의적인 생체중 증가를 보였다. 인산시비량에 따른 고추의 AMF 감염율은 두 종 모두 인산수준이 낮을수록 높은 경향이었고 고추의 생체중은 무접종에 비하여 5~34%정도 높았다. 또한 AMF의 접종시 고추의 무기성분은 $P_2O_5$, $K_2O$의 함량이 뚜렷이 증가하였으며 특히 인산과 칼륨은 지상부에 비해 지하부에서의 증가가 더욱 뚜렷하였다. 이상의 결과로 보아 고추의 육묘시 AMF를 접종하면 지하부의 발달로 인한 고추이식시 근부손상의 감소와 이식직후 묘의 활착회복 및 균일성의 증가로 생육촉진효과가 증대될 것으로 기대되었다.