• The Plant Pathology Journal
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• 제28권1호
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• pp.107-113
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• 2012

Trichomes are specialized epidermal structure having the functions of physical and chemical block against biotic and abiotic stresses. Several studies on $Capsicum$ species revealed that virus and herbivore resistance is associated with trichome-formation. However, there is no research on the structural characterization of trichomes developed on the epidermis of $Capsicum$ spp. Thus, this study attempts to charaterize the trichome morphologies in 5 species of $Capsicum$ using a Field Emission Scanning Electron Microscopy (FESEM). Six main trichome types were identified by their morphology under FESEM. Both glandular and non-glandular types of trichomes were developed on the epidermal tissues of $Capsicum$ spp. The glandular trichome were further classified into type I, IV and VII according to their base, stalk length, and stalk. Non-glandular trichomes were also classified into type II, III, and V based on stalk cell number and norphology. Almost all the species in $C.$ $chinense$ and $C.$ $pubescens$ had glandular trichomes. To our knowledge, this is the first study on classification of trichomes in the genus $Capsicum$ and, our results could provide basic informations for understanding the structure and function of trichomes on the epidermal differentiation and association with biotic stress tolerance.

• 식물조직배양학회지
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• 제28권3호
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• pp.141-146
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• 2001

고추 C. anuumm, C. bacatuum, C chacoense의 하배축, 자엽 및 본엽을 원형질체 분리재료로 하여 실험한 결과, 원형질체 분리를 위해서 세포벽 분해효소로 1% Cellulysin과 0.25% Macerozyme 효소의 조합과 삼투조절제로 0.65 M sorbitol을 사용하였다. 항산화제인 MES를 효소용액에 첨가하여 줌으로써 원형질체 분리과정에서 발생하는 갈변을 억제시킬 수 있었다. 원형질체를 수정된 K8p 배지에 1∼5 mg/L zeatin, 0.5 mg/L IAA, 0.1∼0.5 mg/L TDZ, 1 mg/L 2,4-D를 첨가하여 배양하였을 때 5일에 분열을 시작하였으며 원형질체에서 얻어진 colony들을 agarose가 첨가된 반고체 배지에 고정하여 배양하는 방법이 액체평판배양 방법보다 더 효과적이었으며 이 방법은 세포배양시 분비되는 페놀류 물질로 인한 갈변을 방지할 수 있었다. 세 가지 다른 고추속의 원형질체 배양에서 얻어진 4000여 개의 캘러스를 식물생장조절제인 TDZ, IAA, 2ip, BAP, NAA, zeatin을 이용한 100가지 다양한 조합의 재분화 배지에 옮겼으나 캘러스가 커지거나 고사하는 현상만 관찰되었을 뿐 줄기로 재분화된 식물체는 얻을 수 없었다.

• Mycobiology
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• 제40권2호
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• pp.85-90
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• 2012

A species of Heterobasidion was encountered during a diversity study of endophytic fungi from healthy root tissues of chili pepper (Capsicum annuum L.) in Korea. The fungal species (CNU081069) was identified as Heterobasidion araucariae based on phylogenetic analyses of the internal transcribed spacer and translation elongation factor gene sequences. Morphological descriptions of the endophytic isolate matched well with the previous references and supported the molecular identification. The fungus Heterobasidion araucariae CNU081069 is new to Korea.

• 식품보건융합연구
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• 제4권4호
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• pp.10-17
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• 2018

Capsicum frutescen is known in Sudan, is one of the most commonly used pepper species in cooking and in Sudanese folk medicine. The present study was conducted to investigate antimicrobial (bacteria and fungi) and cytotoxicity (Brine Shrimp Lethality Test) of methanolic extract of Capsicum frutescen (fruits). The extract have been tested in the present study to investigate the in vitro potential effects against Gram positive, Gram negative bacteria and fungi. The selected organisms were Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia and Candida albicans using the cup plate agar diffusion method. The methanol extract of Capsicum frutescen (fruits) exhibited inhibitory effects against Escherichia coli with zone of inhibition (23 mm) and Klebsiella pneumonia with zone of inhibition (17 mm). The phytochemical screening revealed the presence of Tannins, Saponin, Alkaloids, Anthroquinoles and Terpenoids. The Cytotoxicity of methanolic extract of Capsicum frutescens was $LD_{50}$ $64.68{\mu}g/ml$. The activity and presence of compounds known to be biologically active are a validation for the use of Capsicum as a food ingredient and as a therapeutic element of traditional medicine.

• Applied Biological Chemistry
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• 제4권
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• pp.23-28
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• 1963

1. 등전씨(藤田氏)의 Ether-alkali partition extraction법(法)을 개량(改良)하여 Capsaicin을 순수(純粹)하게 분리(分離)하여 정량표준곡선(定量標準曲線)을 얻었다. 2. Soxhlet장치(裝置)를 사용(使用)하여 Ether-Acetone(3:1) 혼합용매(混合溶媒)로 Capsaicin을 추출(抽出)한 후(後) 58% Methanoldmf 전개제(展開劑)로 하는 paper partition chromatography에 의(依)하여 불순물(不純物)로부터 Capsaicin을 순수(純粹)하게 분리(分離)하였다. 3. Diazobenzenesulphonic acid를 발색제(發色劑)로 사용(使用)하는 Schulte-Kruger 변법(變法)에 약간(若干) 개량(改良)을 가(加)하여 각품종별(各品種別) 고추중(中) Capsaicin 함량(含量)을 측정(測定)하였다.

• Journal of Applied Biological Chemistry
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• 제66권
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• pp.320-327
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• 2023

Capsicum annuum belongs to the Solanaceae family, crops of which are extensively cultivated worldwide. It is a food source containing various nutrients and vitamins and also serves as a medicine for treating ailments. The burning feeling experienced while consuming Capsicum fruits is due to the presence of capsaicinoids, particularly capsaicin and dihydrocapsaicin. This study aimed to assess the content of these two compounds in 34 varieties of capsicum and paprika. High-performance liquid chromatography with a gradient elution system and a reverse-phase YMC Pack-Pro column with UV detection at 280 nm was employed. The results revealed that, among the 34 samples, only six samples (samples 1, 15, 20, 29, 32, and 34) contained capsaicin and dihydrocapsaicin, and their highest contents were found in sample 1 - variety name: Sungil-c (capsaicin: 3.42 mg/g extract, dihydrocapsaicin: 1.20 mg/g extract). These findings suggest that the content of these two compounds is attributed to the variety and is influenced by geographical location and environmental factors. Additionally, this study provides a basis for establishing a C. annuum variety with high capsaicin and dihydrocapsaicin contents.

• 원예과학기술지
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• 제16권4호
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• pp.503-507
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• 1998

본 연구는 RAPD표지를 이용하여 국내외에서 수집된 고추 유전자원들간의 유전적관계를 평가하고자 수행되었다. Random primer를 이용한 고추의 PCR반응은 $MgCl_2$ 3mM, Taq. DNA polymerase 1.5U, 주형 DNA 10ng, dNTPs $200{\mu}M$, random primer 200nM 그리고 $42^{\circ}C$의 annealing 온도조건으로 최적화하였다. 80개의 random primer로부터 높은 밴드선명도와 재현성을 보이는 16개가 선발되었으며 70%의 GC함량을 갖는 primer들이 GC함량이 60%인 것보다 DNA증폭에 있어 효과적이었다. 31개의 고추품종및 계통들에 대해 71개의 polymorphic밴드와 22개의 monomorphic밴드를 포함하는 총 93개의 DNA밴드가 선발된 16개의 random primer들로부터 형성되었다. Primer당 약 4.4개의 polymorphic밴드가 형성되었다. 이들 71개의 polymorphic밴드를 이용하여 유사도지수가 구해졌으며 이를 근거로 31고추 계통 또는 품종들을 뚜렷이 구분하는 dendrogram이 작성되었다.

• Molecules and Cells
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• 제27권2호
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• pp.205-209
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• 2009

The loci of the 5S and 45S rRNA genes were localized on chromosomes in five species of Capsicum, namely, annuum, chacoense, frutescens, baccatum, and chinense by FISH. The 5S rDNA was localized to the distal region of one chromosome in all species observed. The number of 45S rDNA loci varied among species; one in annuum, two in chacoense and frutescens, and chinense, and four in baccatum, with the exceptions that 'CM334' of annuum had three loci and 'tabasco' of frutescens gad one locus. 'CM334'-derived BAC clones, 384B09 and 365P05, were screened with 5S rDNA as a probe, and BACs 278M03 and 262A23 were screened with 25S rDNA as a probe. Both ends of these BAC clones were sequenced. FISH with these BAC probes on pachytenes from 'CM334' plant showed one 5S rDNA locus and three 45S rDNA loci, consistent with the patterns on the somatic chromosomes. The 5S rDNA probe was also applied on extended DNA fibers to reveal that its coverage measured as long as 0.439 Mb in the pepper genome. FISH techniques applied on somatic and meiotic chromosomes and fibers have been established for chili to provide valuable information about the copy number variation of 45S rDNA and the actual physical size of the 5S rDNA in chili.

• 한국잡초학회지
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• 제31권3호
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• pp.271-278
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• 2011

The allelopathic effects of Lantana camara L. (Family:Verbenaceae) on germination and seedling establishment of some agricultural crops and weed species have been identified. Aqueous extracts of dry leaves and contaminated soil where L. camara is grown were used to verify allelopathic effect on seed germination of five bioassay species; Raphanus sativas, Capsicum annum, Lycopersicum esculantem, Crotalaria juncia and Chromoleana odorata. Fifty seeds from each bioassay species were placed in a petri dish containing leaf extracts or contaminated soil, and seed germination were examined after 3 days. The plant house experiments were carried out to evaluate the impact of L. camara contaminated soil and leaf debris using L. esculantem as the indicator plant. Seed germination of L. esculentem, C. junica and Capsicum annum was significantly inhibited by L. camara contaminated soil. However, the degree of inhibition varied among the bioassay species. The aqueous extract of dry leaves of L. camara was highly phytotoxic and it significantly reduced seed germination of all bioassay species. There was a decline in plant height, leaf area and shoot dry weight of tomato only in early growth stages when grown in L. camara contaminated soils. However, incorporation of leaf debris into soil affected the vegetative growth of tomato in early stages when the leaf debris concentration was increased. Growth recovered at the latter part of the life cycle. On the basis of these results it can be concluded that the allelochemicals in L. camara contaminated soils are harmful to the seed germination of crop species. The adverse effect was present only during the early growth stages and it did not suppress the latter part of the plant growth. These responses are attributed to allelopathic effects which need confirmation under field conditions.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1998년도 한국생물과학협회 학술발표대회
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• pp.344.2-344
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• 1998

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