• 아시안잔디학회지
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• 제7권1호
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• pp.13-18
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• 1993

Much attention has been given recently to solve the environmental contamination in golf courses Changing to culture practice rather than chemical practice that depends on pesticides and fertilizers is a hot issue in golf courses or grasslands. Organic soil conditioners improve soil-plant envirormental conditions rich in physical properties. In this study, measuring systems to evaluate soil conditioning effects were set up for on-site purpose. After establishing the methodology for evaluating soil conditioner effects, 2 kinds of organic conditioners were rested for examination. The systems for the methodology included a set of simulating equipment for field capacity, an impact type soil column compactor, and an infiltration-percolation system. Test results using the systems showed bulk density and infiltration rate of mixed soil were decreased at highter rates of conditioner, but total porocities were increased. Increased porocities were most capillary pore space which has a positive effect on soil water potential. The systems and methodology in this study seem to have an efficiency to measure the effects of soil conditioner on site purpose.

• 한국미생물·생명공학회지
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• 제49권2호
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• pp.264-268
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• 2021

Tuberculosis (TB) is a major infectious disease that threatens the life and health of people globally. Here, we performed a metabolomic analysis of serum samples from patients with intractable TB to identify biomarkers that might shorten the TB treatment period. Serum samples collected at the commencement of patients' treatment and healthy controls were analyzed using the capillary electrophoresis and time-of-flight mass spectrometry metabolome analysis method. The analysis identified the metabolites cystine, kynurenine, glyceric acid, and cystathionine, which might be useful markers for monitoring the TB treatment course. Furthermore, our research may provide experimental data to develop potential biomarkers in the TB treatment course.

• 공업화학
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• 제7권2호
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• pp.228-236
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• 1996

본 연구에서는 고밀집 섬유다발체 내에 수은과 금속이온을 함유한 수용액을 동시에 주입시켜 최소 공간에서 최대한의 수은전극 면적을 갖는 수직형 순환식 수은 모세관 다발체 전극 전해반응 장치가 개발되었다. 이 장치의 특성과 안정성을 평가하기 위해 수은과 수용액의 유량 변화에 따른 철(III)과 우라늄(VI)이온의 환원 voltammogram이 측정되었다. 수용액의 유속은 장치 내 전해반응에 큰 영향을 미쳤으며, 잘 발달된 한계전류를 얻기 위해서는 정밀한 유속조절이 필요하였다. 측정된 voltammogram의 한계전류는 수용액 유속에 선형적으로 비례하였으며, 한계전류를 보이는 전위에서 연속적으로 우라늄(VI)과 철(III) 이온을 100% 환원시킬 수 있었다. 고밀집 섬유집합체 내에서 수은이 모세관 연속체를 유지하는데 필요로 하는 수은유속 이상에서는 수은유속의 변화는 장치 내의 전해반응에 큰 영향을 미치지 않았다. 본 연구에서 개발된 수직형 순환식 수은 모세관 다발체 전극 전해계는 정확히 제어되는 수용액 유량조건에서 수용액 내의 금속이온의 산화수 상태 및 농도의 연속적인 분석 및 전해반응 기구 해석에 효과적으로 사용될 수 있음을 알 수 있었다.

• 생태와환경
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• 제54권3호
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• pp.247-256
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• 2021

동물플랑크톤이 식물플랑크톤을 선택적으로 섭식하는 특성에 대한 이해는 수생태계 먹이사슬 내의 물질 이동에 중요하다. 하지만 해부를 통한 위내용물 추출 방법은 소형 요각류를 대상으로 적용하기에는 적절하지 않고, 유전자가 유실되거나 위내용물이 아닌 개체 외부의 유전자로 인해 오염될 가능성이 존재한다. 본 연구에서 호 내 식물 플랑크톤 조성 및 기타 환경이 상이한 두 지점을 선정하여 모든 지점에서 지속적으로 출현하는 기수성 요각류인 Sinocalanus tenellus를 대상으로 위내용물의 유전자 분석을 수행하였다. 요각류 개체 외부의 DNA를 제거하는 데 2.5%로 희석한 시판용 표백제(차아염소산나트륨 5.4%)에 2분간 처리하여 증류수로 2회 세척한 뒤 유전자를 추출하였다. 추출된 유전자는 23S rRNA을 증폭하여 서열분석을 실시하였다. Capillary sequencing 분석 결과, 원수와 처리수 및 요각류 위내용물에서 다양한 분류군(규조강, 녹조강, 남조강, 와편모조강, 은편모조강, 황갈조강)에 속하는 식물플랑크톤이 검출되었으며, 새만금호 내 시공간 차이에 따라 상이한 경향을 보였다. 현미경을 이용하여 동정한 식물플랑크톤 군집 조성의 경우 규조강이 우점한 반면, 동일한 원수의 유전자 분석(capillary sequencing) 결과에서는 주로 녹조강, 남조강 및 와편모조강이 우점하여 다소 상반된 경향을 나타냈다. 본 연구에서 적용한 위내용물 분석에 특화된 외부 유전자 제거 전처리 방법은 농도와 처리시간 조절 등의 응용방법에 따라 다양한 동물플랑크톤 분류군에 적용이 가능할 것으로 사료된다.

• 유기물자원화
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• 제27권3호
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• pp.27-39
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• 2019

본 연구에서는 음식물류 폐기물과 하수슬러지의 효과적인 병합 혐기성 처리를 위한 열화학적 가수분해 방법의 최적 조건 평가와, 열화학적 가수분해에 따른 병합 혐기성 소화 효율에 대해 평가 하였다. 열화학적 가수분해는 온도 (80, 100, 120, 140, 160, $180^{\circ}C$)와 NaOH (5, 20, 40, 60, 100 meq/L) 조건에 따른 solubilization COD, CST(Capillary Suction Time), TTF(Time to Filter), volatile fatty acids (VFAs) 등에 대해 평가를 하였으며, 병합 혐기성 소화 효율평가는 biochemical methane potential (BMP) test를 통해 평가하였다. 실험결과 음식물류폐기물과 하수슬러지의 열화학적 가수분해 시 온도 $140^{\circ}C$, NaOH 60 meq/L에서 solubilization COD 20 % 이상, CST와 TTF가 60초 이하, VFAs 농도가 12,000 mg-COD/L 이상으로 최적조건으로 규명되었다. 병합 혐기성 소화 결과도 열화학적 가수분해 조건과 동일한 조건에서 가스발생량이 가장 높았다. 따라서, 음식물류폐기물과 하수슬러지의 효과적인 병합혐기성소화를 위한 열화학적 가수분해 전처리 조건은 온도 $140^{\circ}C$, NaOH 주입농도 60 meq/L라 판단된다.

• 한국환경보건학회지
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• 제23권1호
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• pp.18-27
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• 1997

The isolation, morphological study and growth characteristics of the algae were investigated from Lake Chuam. The isolated algae were applied the Agal Growth Potential test. The method of isolation and purification of the algae were used to Agar plating(AP), nutrient enrichment(NE), dilution(DI) and micro capillary technique(MC). Total isolated algae were 21 species. They were composed of Cyanophyceae, Dinophyceae, Bacillariophyceae, Euglenophyceae and Chlorophyceae. The numbers of algal strain by isolation technique were highest in dilution(21 species), and those of the rests were showed in order of NE > MC > AP. The sizes of isolated Selenastrum and Scenedesmus were $1.8\pm 1.4 \mu m$, $3.3\pm 0.9 \mu m$ in diameter and $6.4\pm 2.3 \mu m$, $13.6\pm 1.9 \mu m$ in length respectively. The morphology of isolated algae and NIES-collection strain was very similar each other, but the size was smaller isolated algae than that of NIES-collection. The optimum culture condition of isolated Selenastrum and Scenedesmus was about 30$\circ$C(25$\circ$C-35$\circ$C) in temperature and the maximum growth was appeared between 7,000 lux and 8,000 lux in the light intensity. The comparison of $\mu$(specific growth rate) on the concentration of nutrients such as nitrate and phosphate, isolated Selenastrum was appeared maximum it at 1.0 mg $NO_3-N/l$ but NIES-collection strain was showed 95% of maximum it at same nitrate concentration. Maximum g of isolated algae and NIES-collection strain in Scenedesmus onto nitrate concentration were very similar with the result of selenastrum. The specific growth rates of isolated algae and NIES-collection strain on the gradient concentration of phosphate were showed 0.72/day and 0.70/day at 0.02 mg $PO_4-P/l$ in Selenastrum but those of Scenedesmus were appeared 0.61/day and 0.57/day at same concentration $PO_4-P$.

• Journal of Periodontal and Implant Science
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• 제30권1호
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• pp.91-104
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• 2000

Many synthetic bone materials have been studied for their potential of regenerative effects in periodontal tissue. Safflower seeds have been traditionally used as a drug for the treatment of fracture and blood stasis in oriental medicines. The purpose of this study was to assess and compare the osseous responses in rat calvarial defects between bone substitutes such as calcium carbonate and bovine-derived hydroxyapatite and feeding of safflower seeds. The calvarial defects were made with 8 mm trephine bur in 24 Sprague-Dawley rats. Two graft materials were implanted in each experimental groups, whereas the control and safflower seed feeding groups were sutured without any other treatment. And then the rats of safflower seed feeding group were supplied with 3 g/day of safflower seeds. Each group was sacrificed at 4 weeks and 8 weeks. To study a histopathology related to bone healing and regeneration, Goldner's Masson Trichrome stain was done at each weeks. The tissue response was evaluated under light microscope. There were more osteoblastic activity, new bone formation, dense bony connective tissues in bovine-derived hydroxyapatite group compared to other groups at 8 weeks. The osseous defect area of safflower seed feeding group was filled with prominent fibrous tissues, where less inflammatory infiltration and new capillary proliferation. In the early phase of bone healing, safflower seed feeding reduces the inflammatory response and promotes the proliferation of connective tissue. These results suggest that natural bovine-derived HA and safflower seed feeding could enhance the regenerative potential in periodontal defects.

• 한국정밀공학회지
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• 제30권6호
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• pp.567-572
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• 2013

In many electro-devices, the vacuum process is used as the manufacturing process. However, the vacuum process has a problem, it is difficult to apply to a continuous process such as a R2R(roll to roll) printing process. In this paper, we propose an ESD (electro static deposition) printing process has been used to apply an organic solar cell of thin film forming. ESD is a method of liquid atomization by electrical forces, an electrostatic atomizer sprays micro-drops from the solution injected into the capillary with electrostatic force generated by electric potential of about several tens kV. The organic solar cell based on a P3HT/PCBM active layer and a PEDOT:PSS electron blocking layer prepared from ESD method shows solar-to-electrical conversion efficiency of 1.42% at AM 1.5G 1sun light illumination, while 1.86% efficiency is observed when the ESD deposition of P3HT/PCBM is performed on a spin-coated PEDOT:PSS layer.

• 대한의생명과학회지
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• 제14권2호
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• pp.97-103
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• 2008

This study was conducted to investigate the effect of an anti-inflammatory and analgesic action of the glucosamine HCl (Glucosamine) and SH-1 (Glucosamine + Oriental herbal composition combined group). Male sprague-Dawley rats $(200{\sim}250g)$ and ICR mice $(20{\sim}30g)$ were randomized and these experimental groups were divided into 4 groups. Two control group were given as negative control (saline) and positive control (Ibuprofen, 100 mg/kg) and two groups given as oral administration of Glucosamine (320 m/kg) and SH-1. Carrageenan induced paw edema test, hot plate method, croton oil induced granuloma, capillary permeability test and acetic acid writhing syndrome were also shown to be comparable in the SH-1 group to anti-inflammatory drug group such as positive control group (Ibuprofen). Although further studies should be performed to confirm the effects of SH-1, present results suggest that the combined administration of SH-1 have potential action in anti-inflammatory and analgesic action. It could be applicable for the improvement of arthritic symptoms as a new diet-supplement.

• 대한세포병리학회지
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• 제17권2호
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• pp.153-158
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• 2006

Low-grade fibromyxoid sarcoma (LGFMS) is a rare soft tissue tumor. There have been only a few prior fine-needle aspiration (FNA) cytological reports. Recognition of this tumor is important because of its potential for metastasis despite its indolent nature and its deceptively bland cytologic appearance. A 60-year-old male presented with a slowly growing mass in the left calf detected 10 years ago. The patient underwent surgical excision. FNA cytology was performed directly on the mass. The smears showed low cellularity composed of hypercellular tissue fragments, hypocellular loose aggregates, and stripped nuclei. The cytoplasm was seen as either collagenous material or very thin fibrillary collagen strands. Tumor cells had spindle, ovoid, or irregular nuclei, fine chromatin, and small nucleoli. Focally slight degree of nuclear pleomorphism is noted. There were no mitotic figures. Blood vessels were frequently seen. Immunocytochemically, tumor cells were negative for S-100 protein, desmin, smooth muscle actin, and CD34. The diagnosis of LGFMS is rarely possible by cytology alone; however, LGFMS should be included in the differential diagnosis of spindle-cell tumors consisting of hypercellular and hypocellular components with some capillary-sized vessels arising in the deep soft tissue of the lower extremities, particularly the thigh. The immunocytochemical findings are of help in the differential diagnosis.