• Bulletin of the Korean Chemical Society
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• v.30 no.2
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• pp.297-301
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• 2009

We have developed the yttrium oxide (YNP) or ytterbium oxide (YbNP) nanoparticle/polymer matrices for the size-dependent separation of DNA ranging from 100 bp to 9,000 bp. High separation efficiency (> $10^6$ plates/m) and the baseline resolution for various DNA standards (100 bp, 500 bp, and 1 kbp DNA ladder) were obtained in 10 min with these matrices. The effects of concentrations of both polyethylene oxide (PEO) and nanoparticles were investigated and the highest performance was obtained at 0.02% PEO with 0.02% YNP or YbNP. Similar sieving power for both YNP and YbNP matrices was observed probably due to the similar sizes of nanoparticles, resulting in the formation of comparable sieving networks for DNA separation. For the reduction of electrosmotic flow, either dynamic or permanent coating of the capillary inner wall was compared and it turned out that PEO was superior to polyvinylpyrrolidone (PVP) or polyacrylamide (PAA) for better separation efficiency.

• Bulletin of the Korean Chemical Society
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• v.32 no.4
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• pp.1361-1364
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• 2011

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.622-622
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• 2005

• Proceedings of the Korean Society of Applied Pharmacology
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• 2007.11a
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• pp.32-37
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• 2007

• Proceedings of the Korean Radioactive Waste Society Conference
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• 2017.10a
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• pp.339-340
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• 2017

• Proceedings of the PSK Conference
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• 2001.04a
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• pp.218.2-218.2
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• 2001

• Proceedings of the PSK Conference
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• 2000.10a
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• pp.248.2-248.2
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• 2000

• Proceedings of the PSK Conference
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• 2002.04a
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• pp.220.1-220.1
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• 2002

• Analytical Science and Technology
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• v.18 no.1
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• pp.35-42
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• 2005

This study reports the effect of $Mg^{2+}$ ions on the behavior of the anionic surfactant in electrophoresis. Intractions of $Mg^{2+}$ ions with carboxylates and sulfonates resulted large changes in their apparent mobilities. A comprehensive analysis of the electroosmotic and electrophoretic mobilities reveals that major cause for the variation of the apparent mobilities of anions arises from the electroosmotic mobilities.

• KSBB Journal
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• v.27 no.4
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• pp.199-206
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• 2012

Chiral crown ethers are synthetic macrocyclic polyethers that bind protonated chiral primary amines with high selectivity and affinity. They have been widely used to separate or distinguish the enantiomers of chiral compounds containing a primary amino moiety by high-performance liquid chromatography, capillary electrophoresis, and NMR spectroscopy. In this paper, two important chiral crown ethers including chiral binaphthyl unit and (18-crown-6)-2,3,11,12-tetracarboxylic acid as chiral selectors are focused. And several chiral resolution techniques and their applications in chirotechnology using these chiral crown ethers with related chiral recognition mechanism studies are reviewed. Especially, it was shown that the commercially available HPLC columns based on (18-crown-6)-2,3,11,12-tetracarboxylic acid have been developed and successfully applied for the resolution of various primary amino compounds including amino acids.