• 대한수의학회지
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• 제38권3호
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• pp.600-605
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• 1998

춘천지역에서 포획한 한국 야생랫드(시궁쥐)에서 Capillaria hepatica에 자연감염된 예를 보고한다. Capillaria hepatica의 충란이 포획된 68마리의 랫드중 17마리에서 발견되었다. Capillaria에 감염된 랫드의 간장은 충란과 함께 림프구, 단핵구, 호산구의 침윤이 관찰되었고 심한 괴사소견을 보였다. 또한 섬유화, 출혈 hemosiderin의 침착이 뚜렷하게 관찰되었다. 최근 국내에서 자연에서 채집된 랫드의 Capillaria hepatica의 감염을 보고한 자료가 없는 것으로 미루어 Capillaria hepatica의 숙주감염을 파악하는 자료로 활용될 수 있을 것이다.

• 대한수의학회지
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• 제38권1호
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• pp.119-128
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• 1998

This study was performed to investigate the pathogenesis of hepatic granuloma and hepatic fibrosis induced in mice infected with Capillaria(C) hepatica and treated cyclophosphamide. The results were as grossly well-defined yellowish white spots and small nodules at the surface of the liver were scattered. Histopathologically, there were numerous granulomas composed of eggs and fragments of C hepatica surrounded by heavy infiltration of inflammatory cells. Severe fibrosis was observed around granulomas. Pathological lesions of group infected with C. hepatica and then injected with cyclophosphamide were most severe than those of other groups. Therefore this study suggested that hepatic fibrosis induced by C hepatica in mice would be useful for animal model of hepatic fibrosis in human.

• 한국수의병리학회지
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• 제1권1호
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• pp.77-79
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• 1997

Capillaria hepatica a primary trichuroid nematode parasite in rodents, has been reported in wide range of secondary hosts including humans. We report a rare case of Capillariasis in a 4-year old raccoon dog(Nyctereutes procyonoides) submitted to the Veterinary Diagnostic Laboratory Chonbuk National University for necropsy on July 5, 1996. Grossly the thoracic cavity contained approximately 200 mls of blood-tinged exudate. Edematous lesion was present around aorta and vena cava. liver was slightly swollen and mottled and contained multifocal yellowish-white lesions ranging from 1 to 3 mm in diameter. Histologically liver lesions were localized around central veins and were characterized by cell swelling and necrosis with mixed inflammatory cells surrounding parasite eggs. These eggs were barrel-shaped with polar caps consistent with Capillaria hepatica.

• Applied Microscopy
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• 제43권4호
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• pp.146-154
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• 2013

Capillaria hepatica (syn. Calodium hepatica) is a parasite found mainly in rodent liver. But, it has also been found in a wide variety of mammals, including humans. This worm is unique as it is the only nematode parasite that is embedded in the liver parenchyma of the host even during the adult stage of the life cycle. They produce eggs that elicit a marked granulomatous reaction that eventually destroys the worms. Fibrosis and lymphoplasmacytic inflammatory infiltration are often observed around adult nematodes embedded in the liver parenchyma of the host. For this reason, complete isolation of this slender worm and observation of the intact ultrastructure is very difficult. In this study, 10 intact whole worms (C. hepatica) were isolated from the liver of 3-week-old mouse after inoculation of artificially embryonated eggs collected from house rats (Rattus norvegicus). Their external structure of was observed with light and scanning electron microscopy. The length of the isolated female and male C. hepatica was approximately 69.60 mm and 36.92 mm, respectively. More detailed ultrastructure, including bacillary band, eggs and vulva in female and spicule and spicule sheath in male C. hepatica was also described.

• 한국동물위생학회지
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• 제20권3호
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• pp.289-295
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• 1997

This study was performed to induced in mice infected with Capillaria hepatica and treated cyclophosphamide. The typical changes of mice were observed hematologically. The results were as follows ; 1. The liver/body weight ratios of mice were Increased to thee peak at the 6th week and then gradually decreased. 2. Hematologically, eosinophils were increased to the peak at the 6th week and then gradually decreased.

• 한국임상수의학회지
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• 제21권3호
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• pp.243-247
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• 2004

This studies was carried out to investigate a process of formation for the granulomatous lesions in the liver and the haematological variation with the lapse of time after infection of Capillaria hepatica in dogs. Twelve crossbred puppies, about 3 months of age and 2-3 kg of body weight, were administered with 2,000 Capillaria hepatica infective eggs. Every four puppies was sacrificed on 1 week, 3 weeks and 5 weeks after infection, respectively. Although no marked clinical sign was noticed, total leukocyte values were increased peak on 1 week, and then reduced gradually on 3 weeks and 5 weeks after infection. Absolute differential counts of neutrophils and lymphocytes were significantly increased on 1, 3 and 5 weeks after infection. Absolute differential counts of monocytes and eosinophils were trend to increase during the experimental periods. On grossly findings, liver congestions were observed in all infected puppies, and a few white specks were scattered under liver capsule in one puppy on 3 weeks and two puppies on 5 weeks after infection. On microscopic findings, many fresh larvae were observed in the liver tissues in one puppy on 1 week after infection. A worm was decayed and only a portion of cuticle was shown in one puppy on 3 weeks after infection. Around the central necrotic material, the layers of thick macrophages with a few giant cells and lymphocytes with fibrous connective tissues were consisted the granulomatous lesions on 5 weeks after infection.

• Parasites, Hosts and Diseases
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• 제52권5호
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• pp.527-529
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• 2014

This study reports the first case of Capillaria hepatica infection in a nutria in Korea. Ten nutrias, captured near the Nakdong River, were submitted to our laboratory for necropsy. White-yellowish nodules were found in the liver of 1 of the nutrias at necropsy. Histologically, the lesions were granulomatous, and infiltrations of lipid-laden macrophages, eosinophils, and several multinucleated giant cells were observed. The lesions consisted of numerous eggs and necrotic hepatocytes. The eggs were lemon-shaped and had polar plugs at the ends of both long sides. The eggs were morphologically identified as those of C. hepatica. Worldwide, C. hepatica infection in nutrias is very rare. Nutrias are a kind of livestock, as well as wildlife; therefore, an epidemiological study for parasitic infections needs to be conducted.

• Applied Microscopy
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• 제39권2호
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• pp.167-173
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• 2009

간모세선충(Capillaria hepatica)은 설치류와 인간을 포함한 포유류의 간에 모세선충증(capillariasis)을 일으키는 기생선충이다. 성충은 모세관과 같이 대단히 가늘고 길며, 충체의 앞부분에는 stichosome (염주체) 및 bacillary band 등의 구조가 있다. 간모세선충은 설치류를 주숙주로 하며, 여러 지역에서 채집된 야생 설치류에 거의 100% 감염률이 보고되었다. 자연계에 존재하는 감염형 자충포장란은 물과 음식에 포함되어 포유류에 감염된다. 감염된 성충은 충란을 배출한 뒤 간 조직 내에서 사멸하고, 죽은 충체와 충란은 간 조직에서 숙주 면역반응을 유발시킨다. 본 연구에서는 집쥐로부터 충란을 수집하여 마우스에 감염형 자충포장란을 감염시키고. 감염 7주 후에 간 조직에 포함되어 있는 성충을 손상되지 않게 분리하였다. 분리된 간모세선충은 주사전자현미경과 투과전자현미경을 이용하여 충체 표피의 미세구조를 관찰하였다. 분리된 간모세선충은 길이가 약 99mm로 확인되었으며, 충체의 표피에는 cuticle, bacillary band 등의 구조물이 관찰되었다. Bacillary band에는 여러 형태의 pore가 분포하였고, pore는 cuticle을 가로질러 존재하며, cap material의 존재 유무에 따라 bacillary pore의 형태적 차이가 나타났다. 간모세선충은 간 조직내에서 성장하는 특성을 가지고 있으므로 손상되지 않도록 성충을 분리해 내기 어렵고, 이에 따라 충체 외부형태에 대한 연구가 용이하지 않았던 것으로 생각한다. 이러한 간모세선충의 성충을 손상없이 분리하고, 표피의 미세구조를 확인함으로써 아직까지 연구가 이루어지지 않은 간모세선충을 포함한 선충류의 형태에 관한 연구에 중요한 기초자료가 될 것으로 생각한다.

• Parasites, Hosts and Diseases
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• 제45권2호
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• pp.95-102
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• 2007

The mRNA expression of several cytokines was evaluated in splenocytes and mesenteric lymph node (MLN) cells of rats infected with Capillaria hepatica by reverse-transcription (RT)-PCR until week 12 after infection. IgG1 and IgG2a, which are associated with Th1 and Th2 response, respectively, were also assessed by ELISA. The results indicated that the majority of cytokines, including the Th1 (IL-2 and $IFN-{\gamma}$ and Th2 cytokines (IL-4, IL-5 and IL-10) were expressed at maximal levels during the early stage of infection (after week 1-2), and the ELISA data also evidenced a similar pattern of changes in IgG1 arid IgG2a. Th1 and Th2 cytokines responded in a similar fashion in this rat model. The expression of cytokines in splenocytes was significantly higher than that in MLN cells, thereby indicating that cytokine production is controlled more by spleen than by MLN. In addition, the observation that $IFN-{\gamma}$ expression increased unexpectedly at the time of maximal egg production (6 weeks after infection) indicated that $IFN-{\gamma}$ is a cytokine reacting against egg production. However, increased IL-5 expression occurring in tandem with worm activity indicated that the activity of C. hepatica might be controlled by IL-5 expression.

• Parasites, Hosts and Diseases
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• 제33권3호
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• pp.235-238
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• 1995

1994년 4월 부터 10월까지 춘천시내 한 동물농장에서 시궁쥐를 잡아 간파 장에서 윤충의 감염상 태를 조사하였다. 모두 43마리 가운데서 간모세선충 11예 축소조충 14예 고양이조충의 조충애벌레(metacestode)가 28예에서 발견되었다. 이 시궁쥐들은 조사 지역의 인수공통감염증의 원천이 될 수 있다.