• 한국수정란이식학회지
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• 제33권2호
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• pp.75-84
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• 2018

The cryopreservation has been extensively applied in many cells including spermatozoa (semen) during past several decades. Especially, the canine spermatozoa cryopreservation has contributed on generation of progeny of rare/genetically valuable dog breeds, genome resource banking and transportation of male germplasm at a distant place. However, severe and irreversible damages to the spermatozoa during cryopreservation procedures such as the thermal shock (cold shock), formation of intracellular ice crystals, osmotic shock, stress of cryoprotectants and generator of reactive oxygen species (ROS) have been addressed. According as a number of researches have been conducted to overcome these problems and to advance cryopreservation technique, several analytical methods have been employed to evaluate the quality of the fresh or cryopreserved canine spermatozoa in regards to the motility, morphology, integrity of membrane and DNA, mitochondrial activity, ROS generation, binding affinity to oocytes, in vitro fertilization potential and fertility potential by artificial insemination. Because the study designs with certain application of analytical methods are selective and varied depending on each experimental objective and laboratory condition, it is necessary to establish the normal reference data of the fresh or cryopreserved canine spermatozoa for each analytical method to monitor experimental procedure, to translate raw data and to discuss results. Here, we reviewed the recent articles to introduce various analytical methods for the canine spermatozoa as well as to establish the normal reference data for each analytical method in the fresh or cryopreserved canine spermatozoa, based on the results of the previous articles. We hope that this review contributes to the advancement of cryobiology in canine spermatozoa.

• Journal of Microbiology and Biotechnology
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• 제23권3호
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• pp.422-429
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• 2013

The aim of this study was to investigate the characteristics of canine uropathogenic Escherichia coli (UPEC) and the interaction between canine UPEC and human bladder epithelial cells. Ten E. coli isolates collected from dogs with cystitis were analyzed for antimicrobial resistance patterns, the presence of virulence factors, and biofilm formation. The ability of these isolates to induce cytotoxicity, invade human bladder epithelial cells, and stimulate an immune response was also determined. We observed a high rate of antimicrobial resistance among canine UPEC isolates. All virulence genes tested (including adhesins, iron acquisition, and protectin), except toxin genes, were detected among the canine UPEC isolates. We found that all isolates showed varying degrees of biofilm formation (mean, 0.26; range, 0.07 to 0.82), using a microtiter plate assay to evaluate biofilm formation by the isolates. Cytotoxicity to human bladder epithelial cells by the canine UPEC isolates increased in a time-dependent manner, with a 56.9% and 36.1% reduction in cell viability compared with the control at 6 and 9 h of incubation, respectively. We found that most canine UPEC isolates were able to invade human bladder epithelial cells. The interaction between these isolates and human bladder epithelial cells strongly induced the production of proinflammatory cytokines such as IL-6 and IL-8. We demonstrated that canine UPEC isolates can interact with human bladder epithelial cells, although the detailed mechanisms remain unknown. The results suggest that canine UPEC isolates, rather than dogspecific pathogens, have zoonotic potential.

• 한국동물위생학회지
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• 제37권4호
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• pp.297-305
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• 2014

Animals and disease frequency of the rescued dogs were investigated in Incheon Veterinary Medical Association Animal Shelter from January in 2012 to December in 2013. Three zoonoses (rabies, brucellosis, and dirofilariosis) and three infectious diseases (canine distemper, canine parvoviral enteritis, and canine influenza) were examined for stray dogs. Among 5,603 heads, 647 (11.5%) went back to their owner and 969 (17.3%) were adopted to new families. Prevalence of dirofilariosis, canine distemper and canine parvoviral enteritis were 2.2% (16/718), 6.0% (24/399) and 6.1% (24/396), respectively. Positive antibody rates against rabies, B. canis and canine influenza virus were 20.5% (41/200), 0.1% (1/718) and 2.0% (4/200), respectively. Protective antibody for canine distemper virus and canine parvovirus were shown in 47.0% (94/200). The data indicate that control measures including facility standards and disease control program are one of the important aspects of the shelter management because stray dogs are exposed to various infectious agents.

• 한국임상수의학회지
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• 제25권6호
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• pp.458-465
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• 2008

The purpose of this study is to characterize canine mesenchymal stem cells (MSCs) derived from bone marrow (BM) for use in research on the applications of stem cells in canine models of development, physiology, and disease. BM was harvested antemortem by aspiration from the greater tubercle of the humerus of 30 normal beagle dogs. Canine BM-derived MSCs were isolated according to methods developed for other species and were characterized based on their morphology, growth traits, cell-surface antigen profiles, differentiation repertoire, immunocytochemistry results, and neurotrophic factor expression in vitro. The canine MSCs exhibited a fibroblast-like morphology with a polygonal or spindle-shaped appearance and long processes; further, their cell-surface antigen profiles were similar to those of their counterparts in other species such as rodents and humans. The canine MSCs could differentiate into osteocytes and neurons on incubation with appropriate induction media. RT-PCR analysis revealed that these cells expressed NGF, bFGF, SDF-1, and VEGF. This study demonstrated that isolating canine MSCs from BM, stem-cell technology can be applied to a large variety of organ dysfunctions caused by degenerative diseases and injuries in dogs. Furthermore, our results indicated that canine MSCs constitutively secrete endogenous factors that enhance neurogenesis and angiogenesis. Therefore, these cells are potentially useful for treating dogs affected with various neurodegenerative diseases and spinal-cord injuries.

• Journal of Animal Science and Technology
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• 제63권6호
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• pp.1411-1422
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• 2021

Lactobacillus acidophilus is a gram-positive, microaerophilic, and acidophilic bacterial species. L. acidophilus strains in the gastrointestinal tracts of humans and other animals have been profiled, but strains found in the canine gut have not been studied yet. Our study helps in understanding the genetic features of the L. acidophilus C5 strain found in the canine gut, determining its adaptive features evolved to survive in the canine gut environment, and in elucidating its probiotic functions. To examine the canine L. acidophilus C5 genome, we isolated the C5 strain from a Korean dog and sequenced it using PacBio SMRT sequencing technology. A comparative genomic approach was used to assess genetic relationships between C5 and six other strains and study the distinguishing features related to different hosts. We found that most genes in the C5 strain were related to carbohydrate transport and metabolism. The pan-genome of seven L. acidophilus strains contained 2,254 gene families, and the core genome contained 1,726 gene families. The phylogenetic tree of the core genes in the canine L. acidophilus C5 strain was very close to that of two strains (DSM20079 and NCFM) from humans. We identified 30 evolutionarily accelerated genes in the L. acidophilus C5 strain in the ratio of non-synonymous to synonymous substitutions (dN/dS) analysis. Five of these thirty genes were associated with carbohydrate transport and metabolism. This study provides insights into genetic features and adaptations of the L. acidophilus C5 strain to survive the canine intestinal environment. It also suggests that the evolution of the L. acidophilus genome is closely related to the host's evolutionary adaptation process.

• 대한소아치과학회지
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• 제50권2호
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• pp.142-154
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• 2023

상악 견치는 기능과 외모에 있어서 중요한 역할을 하지만, 맹출 장애로 인해 문제를 일으키는 경우가 많다. 상악 견치의 매복은 비교적 흔하며 구조적 장애, 발달 이상, 공간 부족, 유전과 같은 다양한 요인에 의해 발생할 수 있다. 상악 견치의 구개측 매복은 서양인에게서 흔한 것으로 보고되지만 한국인에게는 순측 매복이 더 흔한 것으로 관찰된다. 순측으로 변위된 상악 견치의 경우, 치아 총생 및 골격성 부정교합이 보일 수 있으며, 측절치의 선천적 상실이나 과잉 측절치는 상악 견치의 구개측 매복 가능성을 높일 수 있다. 상악 견치 매복의 조기 발견 및 적절한 처치는 조화로운 치열 발달과 정상적인 치아 기능을 위해 중요하다. 본 종설을 통해 매복 상악 견치의 다양한 처치법을 제안하고 그 원리를 설명하고자 한다.

• 한국환경과학회지
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• 제32권12호
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• pp.995-998
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• 2023

This study aimed to determine the effect of feeding Hermetia illucens (H.illucens) pellets with canine diets on fecal properties and digestibility. The canine breeds used in this experiment were nine dogs without discriminating between genders: 3 Maltese (average weight 2.0 kg), 3 Chihuahua (average weight 1.0 kg), and 3 Poodles (average weight 2.5 kg). As canine diets, 70 g of regular diets were mixed with 30 g of H.illucens pellets, and 100 g was weighed on a scale. Dog were fed the experimental diets once a day. No significant changes were observed in the fecal properties in the three types of canines fed a mixture of pellets and canine diets at Days 0 and 7 (p>0.05). At Day 0, the fecal properties of all canines appeared in a less solid state, with scores ranging from 2.00 to 2.22; however, at Day 7, the fecal properties improved to normal (3.00 to 3.33). In addition, the results for nutrient digestibility showed that dry matter and crude fat digestibility were significant (p<0.05); however, crude protein and crude ash digestibility were not affected (p>0.05). In conclusion, these results suggest that feeding a mixture of H.illucens pellets and canine diets to companion canines has beneficial effects on fecal properties and digestibility without adverse effect.

• 한국동물생명공학회지
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• 제38권4호
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• pp.199-208
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• 2023

Background: Canine induced pluripotent stem cells (iPSCs) are an attractive source for veterinary regenerative medicine, disease modeling, and drug development. Here we used vitamin C (Vc) to improve the reprogramming efficiency of canine iPSCs, and its functions in the reprogramming process were elucidated. Methods: Retroviral transduction of Oct4, Sox2, Klf4, c-Myc (OSKM), and GFP was employed to induce reprogramming in canine fetal fibroblasts. Following transduction, the culture medium was subsequently replaced with ESC medium containing Vc to determine the effect on reprogramming activity. Results: The number of AP-positive iPSC colonies dramatically increased in culture conditions supplemented with Vc. Vc enhanced the efficacy of retrovirus transduction, which appears to be correlated with enhanced cell proliferation capacity. To confirm the characteristics of the Vc-treated iPSCs, the cells were cultured to passage 5, and pluripotency markers including Oct4, Sox2, Nanog, and Tra-1-60 were observed by immunocytochemistry. The expression of endogenous pluripotent genes (Oct4, Nanog, Rex1, and telomerase) were also verified by PCR. The complete silencing of exogenously transduced human OSKM factors was observed exclusively in canine iPSCs treated with Vc. Canine iPSCs treated with Vc are capable of forming embryoid bodies in vitro and have spontaneously differentiated into three germ layers. Conclusions: Our findings emphasize a straightforward method for enhancing the efficiency of canine iPSC generation and provide insight into the Vc effect on the reprogramming process.

• 대한수의사회지
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• 제6권1호
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• pp.26-35
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• 1962

A Study on the Treatment of Canine Demodicidosis with Low Toxic Organ Phosphates In this experiment, the treatment of canine demodicidosis with Dipterex(50$\%$ Diethyl trichlorohydroxethyl phosphanate), a kind of low toxic organic phosphates, h

• 대한치과보철학회지
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• 제22권1호
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• pp.79-84
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• 1984

The stability of teeth is an important measure of the periodontal health. This study was designed to determine if there was a relation between the patterns of disclusion and a tooth mobility. An evaluation was made on 117 persons with Angle's class I occlusion who were free from histories of orthodontics, removable or fixed prosthesis, and anterior crowding or open contacts. The results were as follows: 1. In this study, 17.52% of the subjects were exhibited canine protected occlusion. 2. In protrusive movements, 75.21% of the subjects were exhibited incisor guided occlusion and the subjects of canine guided occlusion was the smallest. 3. The canine tooth of mouths having canine-protected occlusions had slightly lower mean tooth mobility scores than the canine of mouths having group function occlsion, but there was no significances. 4. The tooth mobility score of central incisor had higher than that of lateral incisor.