• 대한한방내과학회지
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• 제30권2호
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• pp.355-364
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• 2009

Objectives : Benign prostatic hyperplasia (BPH) is one of the most common diseases among elderly men. Though medicines such as 5${\alpha}$-reductase inhibitor (finasteride) have recently been developed for treating BPH, their adverse effects and low efficacy should not be overlooked. Curcuma longa has a long history of use in traditional medicines of Asian countries. Many reports conclude the component curcumin in Curcuma lonfa, has the potential to treat various diseases including prostate cancer. In this study, we investigated the therapeutic effects and action mechanism of Curcuma longa with a BPH rat model. Methods : Sprague-Dawley rats were used with subcutaneous injection of testosterone after castration, which were histologically similar to human BPH. A total of 30 rats were equally divided into five groups: Group 1 served as control (sham-operated group): Group 2 was the model group: Group 3 and Group 4 animals were administered Curcuma longa at dose levels of 0.5g/kg and 1.0g/kg: Group 5 served as a positive control group and was treated with finasteride at a dose of 1 mg/kg. The drugs were administered orally once a day for 30 days consecutively. After 31 days, the prostates were removed, and analyzed for their prostatic weight and histological examination. Results : The oral Curcuma longa ingestion group showed statistically significant decreases in their prostatic weights compared with the BPH-induced group and the oral finasteride ingestion group (p<0.05). Curcuma longa is also very safe in liver and kidney up to a dose of lg/kg. Injected testosterone histologically led to prostatic hyperplasia in rats, but oral Curcuma longa ingestion decreased this change. Conclusions : These results suggest that Curcuma longa has a definite inhibitory effect on BPH and might be an alternative medicine for treatment and prevention of human BPH.

• Archives of Plastic Surgery
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• 제37권5호
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• pp.519-525
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• 2010

Purpose: Of various effects of relaxin, we assumed that anti-fibrotic effects, neovascularization effects and vasodilatation effects of relaxin might enhance the survival rate of skin flap. In the current study, we used adenovirus expressing relaxin genes to examine whether these genes could enhance the survival rate of a skin flap. Methods: A total of 30 Sprangue-Dawley rats were divided into three groups: RLX group (10; relaxin virus injected group), CTR group (10; no gene coded virus injection group), and PBS group (10; PBS injected group). Each group was intradermally injected with the virus ($10^7$ PFU) and PBS 48 hours before and immediately before the flap elevation. A distally based flap $3{\times}9\;cm$ in size was elevated on the dorsal aspect of each rat. Following this, a flap was placed in the original location and then sutured using a #4-0 Nylon. A surviving area of the flap was measured and then compared on postoperative days 3, 7 and 10. Using a laser Doppler, the amount of blood flow was measured. On postoperative day 10, tissues were harvested for histologic examination and the number of blood vessels was counted. Results: There was a significant increase in the area of the flap survival in the RLX group on postoperative days 3 and 7. The Doppler measurement also showed significantly increased blood flow immediately after the operation and on postoperative days 7 and 10. The number of blood vessels was significantly greater in the RLX group in the tissue harvested on postoperative day 10. The VEGF concentration was significantly higher in the RLX group than others in the tissues harvested on postoperative day 10. Conclusion: Following an analysis of the effects of relaxin-secreting adenovirus on the survival of a flap, the surviving area of the flap and the blood flow also increased. A histopathology also showed an increase in the number of blood vessels and the concentration of VEGF.

• Journal of Preventive Medicine and Public Health
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• 제30권1호
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• pp.17-29
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• 1997

To estimate the prevalence of hepatitis B virus (HBV) and hepatitis C virus (HCV) infection and to determine associated risk factors, a population-based seroepidemiologic study was carried out. In 1993, a health examination survey of the population was carried out in rural area known to have a high incidence of liver cancer. The study population were those who volunteered to participate in a health survey over 10 years of age. Examinees were interviewed by specially trained staffs. Sera from 1,033 study subjects were tested for hepatitis B surface antigen (HBsAg) by .everse passive hemagglutinin (RPHA) estimation and for hepatitis C virus antibody (anti-HCV) by 2nd generation passive hemagglutinin (PHA) estimation. The age and sex standardized prevalence of HBsAg was 6.3% which was similar to national average, but that of anti-HCV was 5.1% which was 4 to 5 times higher than that of blood donors or other health examinees in Korea. In a multivariate analysis, transfusion history, surgical operative history, and acupuncture history were not associated with HBsAg positivity. In contrast, acupuncture history (adjusted odds ratio[OR]=2.2 : 95% Confidence interval[CI] 1.0-4.7) and surgical operative history(adjusted OR=2.0 : 95% CI 1.0-4.1) were associated with anti-HCV positivity. The present study suggest that there is an highly endemic area of HCV infection in Korea and probably this endemicity is associated with a parenteral source of HCV infection other than blood transfusion.

• 한국임상수의학회지
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• 제28권6호
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• pp.610-612
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• 2011

9 년령 암컷 콜리견이 복부 팽만, 식욕 부진 및 변비를 주증으로 본원에 내원하였다. X-ray, 초음파 및 CT 검사를 통하여 복강내 연부조직종괴를 확인하였으나 세포학적 검사에서 종양 세포는 관찰 할 수 없었다. 탐색적 개복술을 계획하였다. 크고 둥근 모양의 종괴가 우측 자궁각에 위치하는 것을 확인하고 난소자궁적출술을 실시하였다. 종괴의 크기는 $40{\times}25{\times}18$ cm 였으며 무게는 9.5 kg 이었다. 조직학적 검사를 통해 낭상자궁내막증식증(CEH)으로 진단하였다. CEH는 자궁 내용물이 감염되거나 자궁 축농증으로 이어짐에도 불구하고 임상 증상이 없는 경우가 많으므로 정확한 진단이 어렵다. 본 증례는 자궁 축농증을 동반하지 않은 매우 큰 크기의 국소성 낭상자궁내막증식증의 드문 예이다.

• 대한수의학회지
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• 제41권4호
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• pp.549-555
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• 2001

Indole-3-carbinol (I3C), one component of cruciferous vegetables (the Fammily of Cruciferae), has been shown to exert its chemopreventive effect in liver, colon and mammary tissue before or concurrent exposure of carcinogen, but there have been several evidences that consumption of I3C induced tumor promotion in some tissues. Our studies were investigated to examine the modifying effects of I3C in the 7,12-dimethylbenz[$\alpha$]anthracene (DMBA) induced rat mammary gland tumor model. Fifty-two female Sprague-Dawley rats were randomly divided into five groups. Animals of the group 1 were given the diet containing 100ppm I3C and animals of the groups 2 and 4 were given the diet containing 300ppm I3C from 6 weeks of age. At 7 weeks of age, the animals of the groups 1, 2 and 3 were intubated with DMBA. All amimals were killed at 20 weeks after carcinogen treatment. There were significant increases of food consumption in I3C feeding groups compared with those of basal diet feeding groups. The incidences of the mammary tumors in the group 1, 2 and 3 were 75.0% (9/12), 56.3% (9/16) and 93.8% (15/16), respectively and the average number of tumors of group 1 (DMBA+I3C 100ppm: $2.08{\pm}0.61$) and 2 (DMBA+I3C 300ppm: $1.19{\pm}0.32$) were significantly lower than that of group 3 (DMBA alone: $4.63{\pm}0.72$) at the value of P<0.05 and P<0.001, respectively. In the pathological examination of appearing tumors, most of them were adenocarcinoma. Many epithelial cells of tumors showed strong estrogen receptor (ER) $\alpha$ expression but there were slight difference of ER $\alpha$ expression among the type of tumors. We suggest that pre-initiation treatment of I3C has an inhibitory effects on mammary carcinogenesis induced by DMBA.

• Journal of Chest Surgery
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• 제35권8호
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• pp.611-615
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• 2002

진폐증은 광물성 분진의 흡입에 의하여 야기되는 폐의 섬유성 변화에 의한 질환이다. 진폐증은 폐에 분진의 축적과 그에 의한 조직의 반응에 의하여 야기되는 것으로 정의되며, 이러한 분진은 대기중의 고형이며 비유기성의 입자라고 생각되어 지고 있다. 상기 질환은 특히 개발도상국의 직업병중 가장 흔하고 중요한 질환이다. 상기 질환의 임상적인 진단하려면 세 가지 조건이 충족되어야 한다: 1) 이학적 검사가 바탕이 되며 임상적으로 증명되는 직업적인 과거력 ; 2) 예전 단순흉부방사선과의 비교 ; 그리고 3) 상기 질환의 진행에 소요되는 시간적 추이에 대한 정확한 이해. 대부분의 진폐증은 매우 느리게 진행하고 임상양상이 나타나기까지는 적어도 수 개월에서 수 년이 걸리기도 한다. 대개의 진폐증은 단순흉부방사선에서 1cm 미만의 다발성의 작고 둥근 결절로 나타나게 된다. 58세의 여자 환자에서 진폐증이 특징적인 직업력이나 분진에 노출된 과거력이 없는 상태에서 우상엽에 $5{\times}4{\times}3$cm크기의 고립성 폐결절로 나타난 진폐증을 우상엽 절제술로 치료하였기에, 본 교실에서는 상기 임상례를 간략한 문헌 고찰과 함께 보고하는 바이다.

• Journal of Nutrition and Health
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• 제45권6호
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• pp.552-561
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• 2012

Metabolic syndrome is a cluster of metabolic risk factors associated with increased risk of cardiovascular diseases. Recently, the prevalence of metabolic syndrome has increased in Korea. The aim of this study was to develop a Healthy Han-sik Nutrition Education Program (HHNEP) based on commonly recognized Korean foods in order to decrease metabolic syndrome risks. The target population of the HHNEP is adults with metabolic risk factors. The initial version of the HHNEP was developed based on a review of literature and various dietary guidelines by expert committees as well as the results of a survey on the perception of common Korean dishes and foods as Han-sik. The focus group discussion conducted of nine participants from the target population. After minor modification, the final version was developed. The contents included five sections: 1) aims, 2) introduction of Korean Food Guidance System, 3) cholesterol contents in food, 4) menu planning method using Han-sik, and 5) examples of daily Han-sik menu. The Han-sik list was selected based on the results from a Han-sik perception answered by 35 Korean adults aged 30-60 years using 517 frequently consumed Korean foods from the fourth Korea National Health and Nutrition Examination Survey. The nutrition education program was developed based on scientific evidence for the prevention of metabolic syndrome and focuses on tailoring education to an individual's dietary problems. Educational method was developed by a group of expert committees based in planned behavior theory and related research results. The education method consisted of assessment of current diet, four face-to-face nutrition education sessions over 8 weeks, and evaluation after 8 and 16 weeks. Initial version of nutrition education materials and methods was tested for feasibility by a select group of nine Korean healthy adults. Successful implementation of the program would include application by nutritional professionals at the health promotion center of the hospitals, public health center, and work sites. Intervention studies are needed to evaluate the feasibility and effectiveness of this program before large-scale applications.

• Archives of Plastic Surgery
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• 제38권6호
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• pp.883-885
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• 2011

Purpose: Nevus sebaceus is a congenital hamartomatous lesion, typically involving head and neck. Various benign and malignant neoplasms can develop in association with nevus sebaceus. We report a case of simultaneous occurrence of squamous cell carcinoma and basal cell carcinoma in nevus sebaceus. Methods: A 73-year-old man presented with erythematous to black verrucous nodules on the right subauricular area. The upper part was accompanied with inflammation and ulceration, and no specific findings suspicious for malignant degeneration were found in the lower part preoperatively. The mass was totally excised and the defect was directly closed. Lymph nodes were not involved on concomitant neck dissection. Results: Histopathologic examination confirmed the presence of squamous cell carcinoma in the upper part and basal cell carcinoma in the lower part of a nevus sebaceus. Negative margins were achieved on resection. No clinical problems were found during the 3-month follow-up period. Conclusion: We experienced a rare case of simultaneous occurrence of squamous cell carcinoma and basal cell carcinoma within the same nevus sebaceus. Because patients with nevus sebaceus have risk of malignant changes, surgical excision and work-up for recurrence and metastasis should be considered in suspicious cases. And even in a totally asymptomatic case, the possibility of occult secondary cancer should be informed before surgery in aged patients.

• 대한한방내과학회지
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• 제32권3호
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• pp.397-410
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• 2011

Objectives : This study was designed to investigate the effect of Injinsammul-tang (IJS) on hepatocellular carcinogenesis in rats. Methods : Sprague Dawley (SD) rats of control and treatment groups received intraperitoneal injection of 50 mg/kg/day diethylnitrosamine (DENA) weekly for 8 weeks. Experimental rats were classified into 3 groups; normal group (Nor), hepatic cancer induced control group (Con), and IJS extract 250 mg/kg administered group (IST) after being injected with DENA. Thereafter the changes of body weight, liver weight and weight of liver/100g body weight, the activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), and superoxide dismutase (SOD) were measured. Gross anatomy and optical microscopy were also observed. Results : The body weight decreased in Con and IST compared with the Nor. The weight of liver and the weight of liver/100g body weight increased significantly in Con and IST compared with the Nor. The activities of AST, ALT, ALP, LDH increased in the Con compared with Nor, but decreased in IST compared with Con. The activities of SOD increased in the Con and IST compared with Nor. Upon naked eye and light microscopic examination, IJS improved the morphological and histopathological changes of liver caused by DENA-induced hepatic neoplasm. The number of hepatic p53 positive cells decreased in the IST compared with Con. Conclusions : Most of the results did not show a significant effect, but some of the results showed a significant effect. It can be estimated that IJS has some effects on hepatocellular carcinogenesis induced by DENA in rats, and further studies will be needed.

• 농약과학회지
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• 제8권2호
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• pp.95-102
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• 2004

현재 국내 사용중이며 내분비계 장애추정농약으로 분류된 benomyl, carbaryl, endosulfan등 17종 농약에 대한 estrogen성 영향을 검색하기 위하여 인체난소암세포(BG1Luc4E2)를 이용한 luciferase assay를 수행하였으며, luciferase assay에서 Eeq를 산출한 후 내분비계 장애추정 농약의 에스트로겐성 영향에 대한 식이섭취 위험도 평가를 실시하였다. Estrogen 수용체 결합시험에서 cypermethrin, dicofol, endosulfan, esfenvalerate 및 fenvalerate가 $10^{-5}$ M에서 최고 영향이 관찰되었고, mancozeb 등 8종 농약은 약한 영향이 관찰되었으며, benomyl 등 나머지 4종 농약은 영향이 없었다. 이들 중 활성이 비교적 강한 dicofol 및 endoeulfan의 1 nmol 17 $\beta$-estradiol에 대한 RLP 와 RLU는 dicofol의 경우 $10^{-5}$ 및 56%이었구, endosulfan은 $10^{-5}$ 및 72%이었다. MRL을 이용한 식이섭취 위험도 평가 결과 농약들의 추청 1일 최대농약섭취량은 cypermethrin 0.667, dicofol 0.1462, endosulfan 0.2066 및 lenvalerate/esfenvalerate 0.2098 mg/person으로 총 추정 1일 최대 농약섭취량이 1.2298 mg/person이었고, 남성 혈중 에스트로겐 증가 농도는 3.075 ng/L로 정상농도에 비해 15%정도 증가하였으나, 국내 모니터링 성적을 기준으로 평가한 결과 남성혈중 에스트로겐 증가 농도는 0.01938 ng/L로 정상농도에 비해 0.09693%정도 증가하였다.