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Risk assessment for estrogenic effect of the suspected endocrine disrupting pesticides  

Lee, Je-Bong (National Institute of Agricultural Science and Technology, RDA)
Shin, Jin-Sup (National Institute of Agricultural Science and Technology, RDA)
Lee, Hee-Dong (National Institute of Agricultural Science and Technology, RDA)
Jeong, Mi-Hye (National Institute of Agricultural Science and Technology, RDA)
You, Are-Sun (National Institute of Agricultural Science and Technology, RDA)
Kang, Kyu-Young (Department of Envrion. Biotechnology, Gyeongsang National University)
Publication Information
The Korean Journal of Pesticide Science / v.8, no.2, 2004 , pp. 95-102 More about this Journal
Abstract
The present study was conducted to test and evaluate estrogenic effect of 17 pesticides including benomy1 and carbaryl, being suspected as endocrine disrupting chemicals. For estrogenic effect examination, luciferase assay were achieved with human ovarian cancer cell, BG1Luc4E2. Estrogenic effects of cypermethrin, dicofol, endosulfan, esfenvalerate, and fenvalerate were observed at the concentration of $10^{-5}$ M by estrogen receptor binding assay. Relative luciferase potency and relative luciferase effects compared with $10^{-10}$ M 17 $\beta$-estradiol were $10^{-5}$, 56% for dicofol, and $10^{-5}$, 72% for endosulfan, respectively. Estimated maximum daily intake for pesticides was calculated from maximum residue limit of agricultural commodity and food consumption was 1.2298 mg/person. Theoretically calculated blood estrogen level from dietary intake for pesticides based on MRL in Korea, 3.075 ng/L was equivalent to 15% of estrogen concentration in normal blood, but practical monitoring data, 0.01938 ng/L was equal to 0.09693% of estrogen concentration in normal blood.
Keywords
Suspected endocrine disrupting pesticides; luciferase activity; risk assessment;
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