• 대한수의학회지
• /
• 제28권2호
• /
• pp.345-353
• /
• 1988

The present study was carried out to investigate the incidence of C jejuni and C coli in chicken. Also were examined the pathogenicity of the isolates in chick by experimental inoculation. Thermophilic Campylobacter were isolated from 34(45.9%) of the 74 specimens, and classified as 21.6% C jejuni, and 24.3% C coli. In the biotyping of 16 stranis of C jejuni isolates, 37.5% of the strains were grouped as biotype I, 62.5% as biotype II. In the case of 18 strains of C coli isolates, 49.9% of isolates were grouped as biotype I, 55.6% as biotype II. n oral inoculation with $10^4cfu$ of Campylobacter isolates into infant chicks(1 to 3 days-old), 17 days-old and 34 days-old chicks, 32.5% of the chicks developed diarrhea on day 1, 52.5% on day 3, 70.0% on day 5, and 27.5% on day 7, and the peak incidence of diarrhea was reached on day 5. The organisms were found to be discharged in feces one day afterwards. C jejuni and C coli strains were detected from the feces in 87.5% of the chicks on day 5. The organisms were multiplied from $10^4$ to $10^6cfu/gm$ in feces 5 to 7 days after inoculation. C jejuni and C coli recovered from 100% of the cecum, 64.3% of the duodenum, 50.0% of the spleen, 42.9% of the livers, and from 21.4% of gallbladders 7 days after inoculation.

• Journal of Microbiology and Biotechnology
• /
• 제27권11호
• /
• pp.1942-1951
• /
• 2017

Campylobacter jejuni and Campylobacter coli are important foodborne pathogenic bacteria, particularly in poultry meat. In this study, the presence of extracellular DNase activity was investigated for biofilm-deficient Campylobacter strains versus biofilm-forming Campylobacter strains isolated from chickens, to understand the relationship between extracellular DNase activity and biofilm formation. A biofilm-forming reference strain, C. jejuni NCTC11168, was co-incubated with biofilm non-forming strains isolated from raw chickens or their supernatants. The biofilm non-forming strains or supernatants significantly prohibited the biofilm formation of C. jejuni NCTC11168. In addition, the strains degraded pre-formed biofilms of C. jejuni NCTC11168. Degradation of C. jejuni NCTC11168 biofilm was confirmed after treatment with the supernatant of the biofilm non-forming strain 2-1 by confocal laser scanning microscopy. Quantitative analysis of the biofilm matrix revealed reduction of extracellular DNA (16%) and proteins (8.7%) after treatment. Whereas the biofilm-forming strains C. jejuni Y23-5 and C. coli 34-3 isolated from raw chickens and the C. jejuni NCTC11168 reference strain showed no extracellular DNase activity against their own genomic DNA, most biofilm non-forming strains tested, including C. jejuni 2-1, C. coli 34-1, and C. jejuni 63-1, exhibited obvious extracellular DNase activities against their own or 11168 genomic DNA, except for one biofilm non-former, C. jejuni 22-1. Our results suggest that extracellular DNase activity is a common feature suppressing biofilm formation among biofilm non-forming C. jejuni or C. coli strains of chicken origin.

• 한국축산식품학회지
• /
• 제24권1호
• /
• pp.92-96
• /
• 2004

사료원료로 활용이 기대되는 육계 내장 사일리지의 $25^{\circ}C$ 저장 중 설탕 첨가에 따른 pH와 가금류와 관련된 주요 병원 성균인 Campylobacter spp., Salmonella spp.와 지표균인 E. coli의 성장 변화를 조사하였다. 설탕을 0%와 1% 첨가한 시료는 저장 2일째부터 pH가 높아지기 시작하였지만 3, 4와 5% 시료는 저장 0일째에서 4일째까지 5.2에서 4.0으로 급속하게 감소하였다. 그 이후 조사된 9일째까지 pH는 일정한 수준으로 유지되었다. 시료의 Campylobacter spp.는 일반적인 미생물 성장곡선을 보여주었다. 설탕이 0% 첨가된 시료의 Campylobacter spp.의 수는 4일째에서 8.21 log CFU/mL이었던 반면 3과 5% 시료는 각각 7.56과 7.38 log CFU/mL로 나타났다. Campylobacter spp.는 설탕 농도가 증가할수록 감소하는 경향이었다. 저장 중 0% 시료의 Salmonella spp. 수는 5.8∼6.8 log CFU/mL 사이로 나타났지만 3과 5% 시료에서는 저장 후 2일까지는 6.8∼7.0에서 나타난 후 매우 급하게 감소하여 4일부터 조사한 8일 동안 균이 검출되지 않았다. 초기시료의 E. coli는 5.8 log CFU/mL이었다. 설탕이 첨가되지 않은 시료에서는 2일째까지 감소하다가 이후부터는 log 4.0∼5.0 CFU/mL 사이로 나타났지만 3과 5% 시료의 E. coli 수는 매우 급격히 감소하여 2일째 이후부터 저장기간 8일 동안 검출되지 않았다. 육계 내부장기 사일리지에 설탕을 첨가함으로써 가금류와 관련된 주요 병원성균인 Campylobacter spp., Salmonella spp.와 지표균인 E. coli 성장이 현저히 감소되어 안정성이 확인된 육계 내부장기 사일리지의 사료 원료화 가능성이 증진되었다.표면 단백질에 의해 영향을 받는 것으로 생각된다.다.y facilitated during the re-cooling period. The lipolysis of that at 30℃ was much enhanced with agitation. The lipolysis was much higher when agitated at 30℃ than when agitated at 10℃. The length of agitation time at 30℃ didn't give any effect on lipolysis. 산양유 원유의 지방분해에 미치는 온도활성화 및 교반의 영향에 대하여 검토하였다. 산양유 원유는 2회에 걸친 온도활성화 처리에서 1차 온도활성화 직후 유리지방산 함량이 가장 많이 증가하였으며, 1차 온도활성화 이후의 재 냉각기간이나 2차 온도활성화 시에는 유리지방산 증가량이 높지 않았다. 산양유 원유는 30℃에 가온 하였을 때 10℃나 40℃에 가온한 경우와 비교하여 재 냉각기간 중 지방분해가 가장 촉진되었다. 산양유의 지방분해 정도는 24시간 예냉 한 후 30℃에서 5분간 가온 처리한 처리구가 가장 높았으며, 40℃에 가온한 처리구는 예냉 기간에 관계없이 유리지방산 함량의 변화가 뚜렷하게 나타나지 않았다. 산양유 원유는 30℃에서 5분간 가온 처리한 처리구가 가장 높았으며, 40℃에 가온한 처리구는 예냉 기간에 관계없이 유리지방산 함량의 변화가 뚜렷하게 나타나지 않았다. 산양유 원유는 30℃에서 가온처리와 교반 처리를 함께 하였을 때 가온 처리만을 한 경우 보다 지방분해가 더욱 촉진되었으며, 30℃에서 교반 처리를 행한 경우가 10℃에서 교반 처리를 행한 경우 보다 지방분해가 더욱 촉진되었다. 산양유 원유는 30℃에서 교반 처리 시간이 연장되어도 지방분해는 뚜렷한 증가를 나타내지 않았다.와 표준체중군 여자에게서 가장 높게 나타났다. 5.

• Pediatric Infection and Vaccine
• /
• 제26권1호
• /
• pp.22-31
• /
• 2019

목적: 캄필로박터 장염은 박테리아 장염의 흔한 원인 중 하나로 알려져 있다. 그러나 국내에서 소아에서의 캄필로박터 장염의 빈도와 임상 양상에 대한 연구는 드물다. 본 연구는 단일 병원 소아청소년과 환자에서 경험한 캄필로박터 장염의 빈도와 임상적 특징을 파악하고자 시행하였다. 방법: 2012년 1월부터 2017년 12월까지 을지대학교 을지병원 소아청소년과에 급성 위장관염으로 방문한 18세 이하 소아환자로부터 대변 검체를 확보하였다. 그 중에서 배양 혹은 polymerase chain reaction을 통해 캄필로박터 장염으로 진단된 환자들의 의무기록을 후향적으로 검토하였다. 결과: 총 123명의 환자가 캄필로박터 장염을 진단받았으며 환자의 나이 중앙값은 12 세(사분위수, 8-16세)이었다. 캄필로박터 장염은 일년 내내 발생했지만 주로 6월과 9월 사이에 86명(69.9%)으로 집중적으로 발생하였다. 증상은 설사(97.6%), 발열(96.7%), 복통(94.3%), 구토(37.4%)와두통(34.1%) 순으로발생하였다. 복부컴퓨터단층촬영은 25.2%의사례에서시행되었다. 다른 치료군과 비교했을 때, azithromycin 3일 요법으로 치료하는 것이 더 짧은 입원 기간과 관련이 있었다 (P<0.05). 결론: 캄필로박터 장염은 청소년기와 여름철에 흔하다. 설사 증상을 보이기 전에 심한 복통과 열이 발생하여 병원에 내원하게 된다. 캄필로박터 장염에 대한 올바른 이해를 수반한다면 적절한 진단과 항생제 사용을 통해 장염의 이환시간을 줄일 수 있을 것으로 보인다.

• 대한수의학회지
• /
• 제27권2호
• /
• pp.245-251
• /
• 1987

A total of 145 strains of thermophilic Campylobacter spp. isolated from the fecal specimens of 108 cattle, 120 pigs and 104 chickens. The isolation rates of Campylobacter jejuni from cattle, pigs and chickens were 36.1%, 38.3% and 28.8%, respectively. In the biotyping of 115 strains of C. jejuni, 49.6% were belonged to biotype I, 33.9% biotype II, 10.4% biotype IV and 6.1 % biotype III. Twenty-eight strains of C. coli were 78.6% of biotype I, 21.4% biotype II. Two strains of C. laridis belonged to biotype I and II. One hundred of 105 C. jejuni cultures were typable serologically and represented 13 serogroups Serotype 4, 5, 26, 27 and 36 were encountered most frequently. Eighteen of 23 C. coli cultures were typable serologically and represented 6 serogroups. Serotype 8, 20, 21 and 31 were encountered most frequently. In the comparison of frequency of serotype between animal species, serotypes 4, 30, 5, 26 and 27 were encountered relatively common in the cattle source isolates, serotypes 26 and 36 in the pigs, and 36 and 17 in the chickens. The serotypes of C. coli encountered most frequently were serotype 8 and 31.

• 한국동물위생학회지
• /
• 제36권1호
• /
• pp.57-60
• /
• 2013

This study was carried out to investigate the prevalence and antimicrobial resistance patterns of Campylobacter jejuni isolated from duck feces. In total, 112 (32.9%) isolates of C. jejuni were identified from 430 duck feces samples from September to December, 2010. All isolates were susceptible to telithromycin, whereas majority of the C. jejuni isolates were resistant to azithromycin (18.8%), ciprofloxacin (86.6%), erythromycin (0.9%), gentamicin (15.2%), tetracycline (80.4%), florfenicol (3.6%), nalidixic acid (87.5%), clindamycin (7.1%). As a result, appropriate protocols for antimicrobial agents and strategies to reduce antimicrobial resistance will be needed in the future.

• 한국식생활문화학회지
• /
• 제30권4호
• /
• pp.475-480
• /
• 2015

Jeju citrus, which contains an abundance of calcium and vitamin, was used to develop fermented citrus peel extract. A total of seven probiotic strains were applied to tangerine dermis to select the best growing bacteria in citrus peel extracts. B. longum, B. bifidum, and L. mesenteroides were found to grow best in citrus peel extract culture containing glucose, yeast extracts, peptone, and potassium phosphate. Citrus peel extract culture consisting of 1% yeast extract, 5% peptone, and 0.1% phosphate was the best environment for growth of probiotics. The pH, acidity, and viable cell numbers of these fermented extracts were measured. The initial pH level of fermented extracts with nutrients was 5.25 and dropped rapidly to 3.39 after 72 hours of fermentation. The acidity of fermented extracts increased to 4.08 % after 72 hours of fermentation, and the viable cell number in fermented extracts after refrigeration for 2 weeks was $1.3{\times}10^{10}CFU/mL$. The antimicrobial activity of citrus peel fermented extracts against Campylobacter jejuni was determined, and concentrations more than 25,000 ppm showed antimicrobial activity.

• Journal of Microbiology and Biotechnology
• /
• 제34권5호
• /
• pp.987-993
• /
• 2024

Campylobacteriosis is a significant foodborne illness caused by Campylobacter bacteria. It is one of the most common bacterial causes of gastroenteritis worldwide, with poultry being a major reservoir and source of infection in humans. In poultry farms, Campylobacters colonize the intestinal tract of chickens and contaminate meat during processing. Vaccines under development against Campylobacters in poultry showed partial or no protection against their cecal colonization. Therefore, this review will elaborate on campylobacteriosis and emphasize the control strategies and recent vaccine trials against Campylobacters in poultry farms. The epidemiology, diagnosis, and treatment of Campylobacter infection, along with specific mention of poultry Campylobacter contamination events in Malaysia, will also be discussed.

• 대한수의학회지
• /
• 제27권2호
• /
• pp.227-233
• /
• 1987

This study was conducted to determine the epidemiological characteristics of Campylobacter enteritis. A total of 187 fecal specimens of Korean native goat were examined for the presence of C. jejuni and C. coli by direct plating. Fifty strains isolated were examined for biochemical and serological properties and susceptibility to 19 chemotherapeutic agents. A total of 29(15.5%) C. jejuni and 21 (11.2%) C. coli were isolated from the fecal specimen of 187 Korean native goats. Of the 50 isolates of C. jejuni and C. coli, 29 isolates of C. jejuni grouped as 7 biotypes (1,2,3,4,6,7 and 8) and biotypes 1(34.5%), 2(17.2%) and 3(20.7%) were encountered most frequently. Twenty-one C. coli strains were differentated into biotype I (61.9% of the isolates) and biotype II (38.1%). Of the 29 C. jejuni strains examined, 24(83.0%) were typable by the Lior serotyping scheme and five isolates were non typable. C. jejuni grouped as 8 serotypes, serotype 4(24.1%) and 26(20.7%) were encountered most frequently. In the case of 21 strains of C. coli grouped as 6 serotypes, the most frequent serotypes were 21(28.6%) and 25(23.8%). Total of 50 strains of isolated were all susceptible to amikacin, clindamycin and tobramycine. Overall 85% of isolates were sensitive to erythromycin, doxycycline, chloramphenicol, flume-quine, kanamycin, gentamicin, nalidixic acid, polymyxin B, colistin, tetracycline and ampicillin, but about 65% of isolates were resistant to cefamandole and ethyl hydrocuprein hydrochloride.

• 대한수의학회지
• /
• 제29권3호
• /
• pp.303-313
• /
• 1989

To investigate the epidemiological trait of intestinal diseases of animals caused by thermophilic Campyllobacter spp., isolation of etiological agent was carried out and the profiles of plasmids and the transfer of resistance plasmid in the isolated Campylobacter spp. were examined. The results were as follows. 1. A total of 110 isolates of C jejuni and C coli were subjected to the test for the presence of plasmid DNA. Of the isolates examined, 60% of the isolates were noted to harbor plasmid DNA. Plasmid occurrencer ate from pigs, chickens and cattle were 76.2%, 61.7% and 37.7%, respectively. The plasmids of a large molecular weight, ranging from 36 Md to 86Md, were identified with the strains of tetracycline resistant. 2. Transfer frequency of tetracycline resistant plasmids was higher in the case of the filter mating method than in the broth mating method by the factor of 10~1,000. 3. Tetracycline resistant plasmids of C jejuni were transferrable to C jejuni and C coli by conjugation. In a low frequency, the transfer of tetracycline plasmid was also possible to Vibrio parahemolyticus. However, it was impossible to transfer to Streptococcus fecalis, E coli and Vibrio cholerae. 4. Tetracycline resistant plasmids of C jejuni were impossible to transfer to Campylobacter spp. and related bacteria by transformation.