• Biomedical Science Letters
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• v.28 no.3
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• pp.206-210
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• 2022

Calystegia dahurica (Herb.) Choisy is a natural product that has not been studied for efficacy or active ingredients. The purpose of this study is to investigate the activation effect of natural killer cells using a natural extract composition based on Calystegia dahurica (Herb.) Choisy extract (CDCE). We evaluated the activity of natural killer cells in natural products using PBMCs from healthy participants. All natural products were extracted with 50% ethanol. Based on the results of the cell viability assay, PBMCs of healthy participants were treated with extracts at various concentrations. Then, analysis was performed using flow cytometry to measure the cd107a surface expression of natural killer cells. As a result, treatment with a single extract of PBMCs increased the expression of cd107a in a concentration-dependent. Furthermore, it was confirmed that the treatment of the extract composition showed the highest expression of cd107a. In conclusion, it is expected that the extract composition containing CDCE according to this study can be used for prevention or treatment of cancer cells, tumor cells, and immune diseases.

• Korean Journal of Plant Taxonomy
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• v.41 no.4
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• pp.338-344
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• 2011

Molecular phylogenetic studies were conducted to evaluate evolutionary trends, relationships and species identities among four species, one variety and one outgroup of the Korean Genus Calystegia. The important characteristics of Calystegia are the shape of the lamina, the length ofthe corolla and the presence of hair. However, many variations were observed as regards the characteristics of the leaf, making true identification difficult. In molecular phylogenetic studies, C. soldanella formed one clade, and it was located mostly in the base. C. hederacea and C. sepium did not form an independent clade in their ITS regions and psbA-trnH regions, and this investigation could not confirm a relationship. Therefore, a relationship between these two species is not sufficiently supported by these markers (ITS and psbA-trnH). Consequently, this research should be achieved through many samples and markers. C. sepium var. japonica and C. dahurica are closely related.

• Journal of Plant Biology
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• v.27 no.1
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• pp.33-41
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• 1984

The relationships and character variations on 5 taxa of Calystegia were examined by sluster analysis and principal component analysis. Thirteen Calystegia population samples from the middle part of Korea were observed. Although minor differences were noted, essentially similar results were obtained from the phenograms by UPGMA, UPGMC and Ward's clustering methods, and these results were in accordance with those obtained from the ordination plots by principal component analysis. C. soldanella is distantly connected with the other taxa mainly because of its morphologically different leaf organs. Based on the difference on the first principal component, C. hederacae is kept apart from the rest 3 taxa. In the relationships among C. japonica, C. sepium var. americana and C. davurica, mivor differences were obtained from the 3 clustering methods. As to the character variations among different populations within a taxon, they are slight in C. soldanella and C. sepium var. americana, but remarkable in C. hederacae and C. davurica.

• Journal of Environmental Science International
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• v.22 no.2
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• pp.187-193
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• 2013

Dry matter allocation characteristics of Calystegia soldanella, grown in pots, was analysed to assess its plasticity in response to water-stressed conditions. As water was withheld leaf water potential between the two watering treatments was similar during the first 6 days, followed by a rapid decrease in water-stressed plants. The minimum leaf water potential was -1.50 MPa on day 15 and the maximum leaf water potential was about -0.5 MPa on day 0 in water-stressed plants. In well-watered plants leaf water potential was maintained almost consistently throughout the experiment. There was no significant difference in plant dry weight between the two watering treatments for 9 days after the start of experiment and that was remarkably increased thereafter, compared with that remained without any increase in water-stressed plants. In dry mass partitioning, however, the water-stressed plants showed a great plasticity, showing that there were 1.81, 1.35 and 0.81 times increase in root, stem and leaf, respectively. Dry mass partitioning in well-watered plants varied from 2% to 5%. The difference of dry mass partitioning between the two watering treatments was reflected in leaf mass per unit area (LMA) and root/shoot (R/S) ratio. LMA in water-stressed plants was lower than that in well-watered plants, while R/S ratio in water-stressed plants was higher in well-watered plants. This means that the water-stressed plants reduced its leaf area and increased dry mass partitioning into root and stem during the progress of soil drying. These results indicate that Calystegia soldanella inhabiting in sand dune cope with water stress with high plasticity which can adjust its dry mass partitioning according to soil water conditions.

• Journal of Plant Biology
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• v.38 no.2
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• pp.173-180
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• 1995

We investigated levels and distribution of genetic variation in Korean populations of Calystegia soldanella and C. japonica, clonally reproducing herbaceous perennials. Calystegia soldanella is one ofecologically important beach plants growing only on sand and beach dunes in Europe, East Asia, the Pacific Islands, and the west coast of North America. In contrast, C. japonica usually grows on small mounds of paddy fields, roadsides, and waste places with patchy distribution. Starch gel electrophoresis was conducted on leaves collected from 13 populations of C. soldanella and eight populations of C. japonica. The levels of genetic variation of the two species are very comparable; means of expected heterozygosity (Hep) were 0.100 and 0.099 for C. soldanella and C. japonica, respectively. These values were also very similar to those for species with similar life-history and ecological traits. However, the proportion of total genetic diversity partitioned among populations (GST) of C. soldanella (0.146) was considerably lower than that of C. japonica (0.383). In addition, means of Nei's genetic identity (Ⅰ) for C. soldanella and C. japonica were 0.985 and 0.900, respectively, which supports a restricted gene flow resulting from obligate clonal reproduction of C. japonica. Significant differences in allele frequency were detected among populations at eight and nine of nine polymorphic loci for C. soldanella and C. japonica (P<0.01), respecitvely. Considering the ecological importance of C. soldanella, the isolated beach populations coupled with present destruction of natural habitats of the species may result in erosion of genetic diversity in the near future. In this respect, conservation efforts should be focused on those populations that currently maintain the most genetic diversity such as those populations in the eastern and southeastern Korean Peninsula and Hamduck Beach, Cheju Island.

• Korean Journal of Weed Science
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• v.4 no.2
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• pp.149-153
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• 1984

The growth of Calystegia japonica Choisy as affected by pH, drying, burial, soil moisture and light intensity was determined. Germination of C. japonica rhizome was not affected by pH's ranging from 4.8 to 8.7,while the greatest growth after germination was obtained at pH 5.7. Drying longer than 14 h at $35^{\circ}C$ brought about a significant reduction in percent survival and the subsequent growth of C. japonica. A significant decrease in growth of C. japonica occurred when the rhizome was buried at 0 ㎝ and deeper than 8 cm. The greatest growth was obtained when the soil moisture content reached 40 to 60% of saturated soil. Increasing percent available light resulted in decrease in the plant height, but increase in the root length and dry weight.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.13 no.2
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• pp.24-41
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• 2010

Distributional characteristics of vascular plants and plants selection for revegetation in bare ground of lakeside was investigated in nine artificial lakes. The vascular plants were composed of 64 families, 194 genera, 268 species, 3 subspecies, 30 varieties, 3 forma, totaling 304 taxa. Dominant species of nine investigated areas were represented as Ambrosia trifida, Calystegia hederacea, Matricaria matricariodes, Xanthium italicum. Dominant species of three parts based on the location of artificial lakes were as follows:Ambrosia trifida, Bidens tripartita, Calystegia hederacea, Equisetum arvense, Setaria viridis, Spergula arvensis, Torilis japonica in northern part (Lake Paro and Soyang); Calystegia hederacea, Matricaria matricariodes, Rumex crispus, Xanthium italicum in middle part (Lake Chungju, Daecheong, Andong and Imha); and Ambrosia artemisiifolia, Bidens frondosa, Chenopodium glaucum, Miscanthus sinensis var. purpurascens, Persicaria pubescens, Setaria viridis in southern part(Lake Juam, Hapcheon and Jinyang). Among the 304 taxa, Korean endemic, endangered and rare plants were not investigated. The naturalized plants were 46 taxa, and life form was Th-$R_5-D_4$-e type. Above the results, Calystegia hederacea, Equisetum arvense, Xanthium italicum, Matricaria matricariodes, and Spergula arvensis were considered with proper species for revegetation in bare ground of lakeside in artificial lakes.

• Korean Journal of Medicinal Crop Science
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• v.14 no.6
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• pp.347-353
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• 2006

This study was conducted to evaluate the vegetation structure and ordination of the Peucedanum japonicum Thunb. community by the Phytosociological method, floristic composition table on east coast of South Korea. The vegetation of Peucedanum japonicum Thunb. community was classified into 2 communities such as the Aster Spathulifolius community and the calystegia soldanella-Artemisia princeps community. Vegetation of the Peucedanum japonicum Thunb. community with the Aster spathulifolius community was shown southward, whereas vegetation of Peucedanum japonicum Thunb. community with the calystegia soldanella-Artemisia princeps community was shown northward from the base line of Pohang at North latitude of 36" 05'. Accordingly, the Peucedanum japonicum Thunb. community was grown commonly with the Aster spathulifolius community southward of the base line of Pohang, whereas it was grown commonly with the calystegia soldanella-Artemisia princeps community northward on the east coast. All environmental conditions of habitat taken together, the optimum habitat of Peucedanum japonicum Thunb. was at Ulsan geographically and the middle region of sea cliff topography, and was suitable for alkali. sandyloam.

• Biomedical Science Letters
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• v.15 no.4
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• pp.335-342
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• 2009

This study was investigated to evaluate the contents of polyphenols and flavonoids, and physiological activities of various extracts from Calystegia japonica roots for making good use of their functional materials. The roots of C. japonica were extracted with water (WE), ethanol (EE) and hot water (HWE) by different methods. Among these extracts, the highest extracting yield was 30.30% of HWE, while the highest contents of total polyphenols and flavonoids were 40.85% and 6.40% of WE, respectively. The nitrite scavenging abilities were ranged from 31.31% (HWE) to 37.46% (EE) at pH 1.2 and 1.0 mg/ml concentration. In the measurements of electron donating abilities, EE showed the highest effect as 91.83% at 0.3 mg/ml assay concentration, and the electron donating ability was decreased as the extract concentration was increased. In the results of superoxide dismutase (SOD)-like activity, HWE showed the highest effect as 7.15% at 1.0 mg/ml. The tyrosinase inhibition activities of WE and EE were 15.28% and 14.97%, respectively. The xanthine oxidase inhibitory effects were ranged from 97.50 to 99.28% at 1.0 mg/ml. These results indicate that C. japonica extract has a good antioxidant effects and could be useful for developing functional products.

• Journal of Ecology and Environment
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• v.30 no.2
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• pp.135-142
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• 2007

We used the Braun-Blanquet method to study coastal dune vegetation of South Korea. Coastal vegetation was monitored in thirty sites from April 2004 to September 2005. Seventeen plant communities were classified into five groups as follows: A. Two associations of herbaceous sand dune plants in Salsoletea komarovii Ohba, Miyawaki et Tx. 1973 (Calystegia soldanella community, Lathyrus japonicus-Calystegia soldanella community), B. Twelve associations of herbaceous perennial sand dune plants in Glehnietea littoralis Ohba, Miyawaki et Tx. 1973 (Carex pumila community, Elymus mollis community, Vitex rotundifolia-Elymus mollis community, Ixeris repens community, Elymus mollis-Ixeris repens community, Lathyrus japonicus community, Messershmidia sibirica community, Glehnia littoralis community, Carex kobomugi community, Calystegia solda-nella-Carex kobomugi community, Ishaemum anthephoroides community, Zoysia macrostachya community), C. One association of shrubby perennial sand dune plant in Viticetea rotundifoliae Ohba, Miyawaki et Tx. 1973 (Vitex rotundifolia community), D. One association of shrubby perennial sand dune plant in Rosetea multiflorae Ohba, Miyawaki et Tx. 1973 (Rosa rugosa community), E. The naturalized community (Diodia teres community).